FastQCFastQC Report
Wed 25 May 2016
SRR1780507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287347
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA250.002345428835.2133941
TTAATAC401.8366347E-532.943613
CGACAAT353.1719828E-431.49545717
ATGCGAC353.1784907E-431.48445914
GTACATT300.00570763829.3649838
TGGATTC300.00576630929.30361428
CGCATTT300.00577613429.2934141
CCACGAC300.00578597229.2832139
CGAAACT554.9694227E-627.98137525
CGTTACC406.946436E-427.54889914
CGTTTAC406.946436E-427.54889914
CCGTTTA406.9535326E-427.54408813
ATCCCGT407.0033787E-427.5104641
ATGCTAT508.298159E-526.4100461
GTTAATA701.1424399E-625.1042652
TGCGACA450.001377029924.50074815
TCGTTAC450.00138264924.48363513
GTCGTTA450.001385465424.47508812
GCCGTTT450.001385465424.47508812
TATAGGT450.001403888224.41967630
Produced by FastQC (version 0.11.5)