FastQCFastQC Report
Wed 25 May 2016
SRR1780507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287347
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT235838.207150239953783No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT187466.523819632708886TruSeq Adapter, Index 18 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC56381.96208765012337No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC33391.1620096955945252No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG29731.0346375636425647No Hit
TATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT16540.5756106728102259No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC15020.5227129568083189No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC10310.3587996394603041No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG8460.2944175509053514No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC6850.2383877332980682No Hit
CTTAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC6050.21054683013916972No Hit
CTTATAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG5250.18270592698027124No Hit
TTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC4480.15590905768983146No Hit
CTTATTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG3230.11240764650405259No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG3190.11101560134610766No Hit
CTTATCACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC2890.10057526266152074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAC207.838052E-444.0097513
TTGTACT207.838052E-444.0097514
CCGTCTT19900.042.45427744
TTTACAC6650.038.0469172
GCCGTCT22500.037.54844743
CTTTACA6800.037.227091
CTATACA3850.037.164031
GGTTATC301.294318E-436.6875651
AGCGTTA301.296974E-436.6747910
CGCTTCT301.2983035E-436.66840735
GACACAT301.2996343E-436.66202526
GGGACCA250.002349270535.201679
TCTTTAT1450.033.3983351
GCCCAGT401.8168434E-532.99582319
AGACCGG401.8168434E-532.9958237
TTACACA7700.032.8587043
CTTATAC34100.031.5018181
GTTTGCA353.2076339E-431.43553511
GCGTTAT353.2076339E-431.43553511
CGTTATG353.2076339E-431.43553512
Produced by FastQC (version 0.11.5)