FastQCFastQC Report
Wed 25 May 2016
SRR1780506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61949
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG150.00221555469.99515518
AGAGTGT150.00221555469.99515565
ATATAGA150.00221555469.9951556
TACGAGT150.00221555469.9951554
GTACTGG150.00221555469.99515538
GCACTTG150.00221555469.99515550
AGTCGTA150.00221555469.99515520
CACTGCT252.3741579E-455.99612442
ATATTAG200.00679115752.752031
TTGGCGG200.006923054352.4963658
GCGTTGG200.006923054352.4963657
GTTACTA200.006923054352.49636519
AACATGG200.006923054352.49636526
ACCAGAG200.006923054352.49636562
TCGTAAC200.006923054352.49636522
ACAAGTC200.006923054352.49636517
CACTTGG200.006923054352.49636551
TGGTAAT200.006923054352.4963659
AAGTCGT200.006923054352.49636519
GTGTACT305.8381533E-446.66343736
Produced by FastQC (version 0.11.5)