##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780506_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61949 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.072995528579963 33.0 23.0 34.0 12.0 34.0 2 29.036643045085473 34.0 23.0 34.0 12.0 34.0 3 29.266186701964518 34.0 27.0 34.0 11.0 34.0 4 29.792781158695057 34.0 31.0 34.0 11.0 34.0 5 29.825404768438553 34.0 31.0 34.0 11.0 34.0 6 31.96442234741481 37.0 31.0 38.0 11.0 38.0 7 30.946036255629632 36.0 27.0 38.0 11.0 38.0 8 30.250786937642253 36.0 26.0 38.0 11.0 38.0 9 31.307285024778448 37.0 27.0 38.0 12.0 38.0 10-11 31.51979854396358 37.0 27.5 38.0 11.0 38.0 12-13 31.841014382798754 37.0 29.0 38.0 11.0 38.0 14-15 31.464067216581384 37.0 27.0 38.0 11.0 38.0 16-17 31.321845388948972 37.0 27.0 38.0 11.0 38.0 18-19 31.353468175434635 37.0 26.5 38.0 11.0 38.0 20-21 31.470306219632278 37.0 27.0 38.0 11.0 38.0 22-23 31.37731843936141 37.0 27.0 38.0 11.0 38.0 24-25 31.77205443187138 37.0 29.5 38.0 11.0 38.0 26-27 31.775121470887342 37.0 27.5 38.0 11.0 38.0 28-29 31.807398020952718 37.0 29.5 38.0 11.0 38.0 30-31 31.65326316808988 37.0 27.0 38.0 11.0 38.0 32-33 31.51532712392452 37.0 27.0 38.0 11.0 38.0 34-35 31.421524156967827 37.0 27.0 38.0 11.0 38.0 36-37 31.366809795154076 37.0 27.0 38.0 11.0 38.0 38-39 31.731795509209185 37.0 27.0 38.0 11.0 38.0 40-41 31.311183392790845 37.0 27.0 38.0 11.0 38.0 42-43 31.65294839303298 37.0 27.0 38.0 11.0 38.0 44-45 30.817688743966812 37.0 26.0 38.0 11.0 38.0 46-47 31.4769003535166 37.0 27.0 38.0 11.0 38.0 48-49 31.323152916108413 37.0 27.0 38.0 11.0 38.0 50-51 31.277429821304622 37.0 26.5 38.0 11.0 38.0 52-53 31.251868472453147 37.0 27.0 38.0 11.0 38.0 54-55 30.991315436891636 37.0 26.0 38.0 11.0 38.0 56-57 30.997853072688827 37.0 26.5 38.0 11.0 38.0 58-59 30.850885405736975 36.5 26.0 38.0 11.0 38.0 60-61 30.96698897480185 37.0 26.0 38.0 11.0 38.0 62-63 30.964131785823824 37.0 26.0 38.0 11.0 38.0 64-65 31.02049266331983 37.0 26.0 38.0 11.0 38.0 66-67 30.981153852362425 37.0 26.0 38.0 11.0 38.0 68-69 30.469862306090494 36.0 24.5 38.0 11.0 38.0 70-71 30.30513809746727 36.0 24.5 38.0 11.0 38.0 72-73 30.30166750068605 36.0 24.5 38.0 11.0 38.0 74-75 30.099420491049088 36.0 24.0 38.0 11.0 38.0 76 29.386301635216064 34.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.1269021227402263 1101 2 -2.1618754187149136 1101 3 -2.1613873299509088 1101 4 -2.983996154273388 1101 5 -2.6469953659951564 1101 6 -4.114789437884017 1101 7 -1.9827897578417826 1101 8 -1.43970439240368 1101 9 -1.9114225597854926 1101 10-11 -2.504008616295309 1101 12-13 -2.632718119706066 1101 14-15 -1.1857018052881045 1101 16-17 -2.5360741644869513 1101 18-19 -1.703903427062798 1101 20-21 -2.720605422055396 1101 22-23 -1.8880371618601721 1101 24-25 -2.733777037127094 1101 26-27 -2.805661356124375 1101 28-29 -2.497609843499145 1101 30-31 -2.450522757311955 1101 32-33 -2.230794080340555 1101 34-35 -2.3301471188173224 1101 36-37 -1.9941277637667483 1101 38-39 -1.937819705058331 1101 40-41 -1.969786021200914 1101 42-43 -2.6966597833246304 1101 44-45 -1.7042581656162952 1101 46-47 -2.1623237399056165 1101 48-49 -3.4311694981821574 1101 50-51 -2.8823527147335994 1101 52-53 -1.8379404092641352 1101 54-55 -2.6131969811015665 1101 56-57 -3.0237375381314457 1101 58-59 -2.6860431892145655 1101 60-61 -2.0079846519119755 1101 62-63 -3.4967557377733094 1101 64-65 -2.930948906181303 1101 66-67 -2.721187248662787 1101 68-69 -2.220090437008839 1101 70-71 -2.0206094361373523 1101 72-73 -2.66048362310411 1101 74-75 -3.094955621236206 1101 76 -2.6433413989645764 1102 1 0.8792948212326088 1102 2 0.6659259395193828 1102 3 1.019172941695949 1102 4 1.33531623961456 1102 5 1.482206671356284 1102 6 1.7393277098239608 1102 7 1.9138571012414118 1102 8 2.376721753606507 1102 9 2.6839085098240183 1102 10-11 0.8037587860816018 1102 12-13 -3.639891294578735 1102 14-15 -0.5806933163237566 1102 16-17 -2.511222721363012 1102 18-19 0.0401967427164891 1102 20-21 1.0914913521381528 1102 22-23 -0.09317298528971563 1102 24-25 1.447929244706522 1102 26-27 -4.289192765292459 1102 28-29 0.21841307670458932 1102 30-31 0.5778728284265888 1102 32-33 -5.040259275586735 1102 34-35 -0.4277277639786128 1102 36-37 0.9226167184064273 1102 38-39 0.6950113645511706 1102 40-41 0.9749720433152156 1102 42-43 1.612678077456355 1102 44-45 -4.093158844733441 1102 46-47 0.8050149697718041 1102 48-49 1.072289754788983 1102 50-51 -4.759135397246336 1102 52-53 -0.7942859101130324 1102 54-55 1.2605933414790798 1102 56-57 -0.6321628352452002 1102 58-59 0.6784941622285565 1102 60-61 0.9337598302612022 1102 62-63 -3.6829823931213532 1102 64-65 0.004492519964706787 1102 66-67 0.15005638461395066 1102 68-69 -6.2614104709646945 1102 70-71 -0.2062206415702903 1102 72-73 0.461884797948521 1102 74-75 -5.1542934820171915 1102 76 -0.7385026892871558 1103 1 0.35874485080091034 1103 2 0.9130430299983878 1103 3 0.6902255732748976 1103 4 0.940505476752346 1103 5 0.8367306216815358 1103 6 -1.3207698655752083 1103 7 1.532795599171628 1103 8 0.848262262181315 1103 9 1.0075335837447703 1103 10-11 1.3097463673944318 1103 12-13 -0.5960562857082401 1103 14-15 0.05192052164194649 1103 16-17 0.29092837266419025 1103 18-19 -0.06883637378262364 1103 20-21 0.46368236337410806 1103 22-23 0.5420280791691816 1103 24-25 0.7170747207561945 1103 26-27 -0.36104374104644776 1103 28-29 0.7257681920209684 1103 30-31 0.5686327338080197 1103 32-33 -3.052160517455455 1103 34-35 -0.358907539259512 1103 36-37 0.5209682855264752 1103 38-39 1.5361926182472132 1103 40-41 1.038876537697238 1103 42-43 1.2575627610755156 1103 44-45 -3.6872230079504753 1103 46-47 0.31835766640102037 1103 48-49 0.49936692805923144 1103 50-51 1.1321271692823522 1103 52-53 0.41465110940204397 1103 54-55 0.7767450268723373 1103 56-57 1.2198108489653272 1103 58-59 0.7916298807383129 1103 60-61 1.1936282513138323 1103 62-63 0.6774921781382552 1103 64-65 1.0844008582260898 1103 66-67 0.4270005005217037 1103 68-69 -2.025418454406445 1103 70-71 0.7750774069217279 1103 72-73 1.6535613207161148 1103 74-75 -0.8047961431644417 1103 76 -0.24692965557929014 1104 1 -3.4351334909271003 1104 2 -0.6038109025858809 1104 3 -0.30977442672510236 1104 4 0.0903827852890906 1104 5 -0.9288641090429905 1104 6 -1.0509777953182038 1104 7 0.38595328998913914 1104 8 0.4993019108962855 1104 9 -0.5748634200005469 1104 10-11 -0.7124995321277154 1104 12-13 0.15313653361243595 1104 14-15 -0.4745227174126825 1104 16-17 -1.2808461333412353 1104 18-19 0.16111325814298638 1104 20-21 -0.2605201421147214 1104 22-23 -0.5433393494760459 1104 24-25 0.32988629249841495 1104 26-27 0.19038981184003134 1104 28-29 -1.0404722832470128 1104 30-31 -0.9294623197887795 1104 32-33 -1.5224885556835304 1104 34-35 -0.5642220168521774 1104 36-37 -0.13217003295473617 1104 38-39 -0.5759732089515452 1104 40-41 -0.1564934739261652 1104 42-43 0.5612564198640975 1104 44-45 -2.574669734025637 1104 46-47 -0.44139307620520896 1104 48-49 0.6435616846135375 1104 50-51 0.44647561303799677 1104 52-53 -0.45494078004648486 1104 54-55 -1.1438606815094268 1104 56-57 0.15084533172394643 1104 58-59 0.4705011192763493 1104 60-61 -0.13970508201950338 1104 62-63 -0.5325362346216558 1104 64-65 -0.1657282906826012 1104 66-67 -0.007876162270495257 1104 68-69 -2.5719443487626386 1104 70-71 -1.3576876712133625 1104 72-73 -0.19956408287422533 1104 74-75 -0.7522971117812567 1104 76 0.5689685750806603 1105 1 -1.8226010474714087 1105 2 -0.8134883219407207 1105 3 -0.5732152869401546 1105 4 -0.7700176596497315 1105 5 -0.7577480541672017 1105 6 -0.6911335239055205 1105 7 -0.8698865320353306 1105 8 -0.7569086934789482 1105 9 -0.21464836623710326 1105 10-11 0.3836257923068409 1105 12-13 -1.9843310229318725 1105 14-15 -0.6539813751805745 1105 16-17 1.048872072072193 1105 18-19 0.21330087401247155 1105 20-21 -0.329207572593031 1105 22-23 -0.2982522156236165 1105 24-25 -0.7117340263744083 1105 26-27 -0.11587640988781445 1105 28-29 -1.0368571553271018 1105 30-31 -0.5908453379571164 1105 32-33 0.2606037691218077 1105 34-35 0.6892077198923552 1105 36-37 1.1381303007493777 1105 38-39 0.24605126268360067 1105 40-41 0.06300967772381583 1105 42-43 -0.18160601988376968 1105 44-45 0.887139683846069 1105 46-47 -0.117700083991636 1105 48-49 -0.47471788527893466 1105 50-51 -0.7033204566690863 1105 52-53 -1.3615963232426296 1105 54-55 -1.0841647230370484 1105 56-57 0.0907785909008112 1105 58-59 -0.631742113945748 1105 60-61 -0.5214255121270313 1105 62-63 0.21023350953852216 1105 64-65 0.3857177604853632 1105 66-67 -0.21903671102837663 1105 68-69 0.658404186647072 1105 70-71 0.11487443483038717 1105 72-73 -0.15887071987830126 1105 74-75 -1.502482502956635 1105 76 -0.9132338720828557 1106 1 -0.5257291022123667 1106 2 0.32649212771714886 1106 3 0.8659831490324734 1106 4 1.0011455857070644 1106 5 0.521919590016573 1106 6 0.7675175024874434 1106 7 1.30831483648862 1106 8 1.1340981491407973 1106 9 1.494833549696427 1106 10-11 1.0327460269109494 1106 12-13 0.5701166799029203 1106 14-15 1.1332620265789544 1106 16-17 1.3108466566274188 1106 18-19 0.38290009199878483 1106 20-21 0.5877034733502775 1106 22-23 1.4418748616002368 1106 24-25 1.1840137742121044 1106 26-27 1.9993239811190229 1106 28-29 0.1451076540570604 1106 30-31 0.347131201632326 1106 32-33 1.8093818727386264 1106 34-35 1.7593934481425997 1106 36-37 1.7241518061257288 1106 38-39 0.556315192302371 1106 40-41 1.0806538614970371 1106 42-43 1.272646179529719 1106 44-45 1.8939823035919154 1106 46-47 1.6955452728021072 1106 48-49 1.1420465330983873 1106 50-51 1.347363805305502 1106 52-53 0.9805346640496495 1106 54-55 1.902638796024533 1106 56-57 1.5789017580562366 1106 58-59 1.047094640697452 1106 60-61 1.3375676452532232 1106 62-63 1.1235766180429216 1106 64-65 1.6400188357541765 1106 66-67 1.984286847133891 1106 68-69 1.8643670409970206 1106 70-71 0.9049037603435863 1106 72-73 0.7576981950777082 1106 74-75 -0.433958553787523 1106 76 0.09937609859163032 1107 1 -0.8853250618083273 1107 2 -0.9166763996619025 1107 3 0.526482883216417 1107 4 -0.1507948714965508 1107 5 0.4617388350989735 1107 6 0.025146423274257756 1107 7 -0.009388512793680803 1107 8 0.9783299407410091 1107 9 -0.7473780398835856 1107 10-11 -0.9952178221055306 1107 12-13 -0.07629480335065963 1107 14-15 -0.10847109410153166 1107 16-17 -0.1548630722402038 1107 18-19 0.650576859675553 1107 20-21 0.8846200071089108 1107 22-23 -0.6055998858745113 1107 24-25 -0.17999473190166526 1107 26-27 0.003204895526256024 1107 28-29 -1.5363383852837167 1107 30-31 -0.4038523177722446 1107 32-33 0.6739220109629684 1107 34-35 0.07592720678162124 1107 36-37 0.06040911606724464 1107 38-39 0.7326575633815864 1107 40-41 0.2589632713853902 1107 42-43 0.12729796049729103 1107 44-45 -0.4794892540901685 1107 46-47 -0.2063154395849196 1107 48-49 -0.3140406545677479 1107 50-51 0.009139456554837722 1107 52-53 0.38298017175831234 1107 54-55 -0.06945051817004355 1107 56-57 0.3236120185559699 1107 58-59 -0.11499998981822657 1107 60-61 -0.25520215804289137 1107 62-63 -0.5174122176827574 1107 64-65 0.10025275388283461 1107 66-67 0.4574394255496266 1107 68-69 0.791586605058697 1107 70-71 -0.060752805312979774 1107 72-73 -0.09440175175907939 1107 74-75 -0.018328569736489442 1107 76 -1.1961050284684411 1108 1 0.07122366569012328 1108 2 0.019094125347081103 1108 3 -0.71759565577538 1108 4 -0.3096043719702699 1108 5 0.49764330763973064 1108 6 0.2784706089712756 1108 7 1.020994707827704 1108 8 1.0699369844209734 1108 9 -0.18473306618304264 1108 10-11 0.5002083597364049 1108 12-13 0.4774638950713701 1108 14-15 0.4892125937027032 1108 16-17 -0.6574113423568377 1108 18-19 -0.5185727368483448 1108 20-21 0.15364219012697689 1108 22-23 -1.1229803948751318 1108 24-25 -0.13296608019791734 1108 26-27 -0.35329155976467064 1108 28-29 0.2443797041670983 1108 30-31 0.39847706247539705 1108 32-33 0.5418856817787443 1108 34-35 -0.08624731705123878 1108 36-37 0.15525050258754902 1108 38-39 0.8860764924547269 1108 40-41 0.6345390824213588 1108 42-43 0.509179106565437 1108 44-45 0.5438045483862268 1108 46-47 0.7048054725650985 1108 48-49 0.34980004587983515 1108 50-51 -0.36465583241246335 1108 52-53 -0.5227665040559906 1108 54-55 -0.624532356844945 1108 56-57 0.6206488377921424 1108 58-59 -0.9920622917555662 1108 60-61 0.7704438937719331 1108 62-63 -0.10875790032512 1108 64-65 0.31487917094970896 1108 66-67 0.7317051643846106 1108 68-69 0.8036255478532972 1108 70-71 2.0112521605467357 1108 72-73 1.5305778294098573 1108 74-75 1.006468058706126 1108 76 0.9528380928357478 1109 1 -1.1592714219615843 1109 2 -1.1670292933904776 1109 3 -1.315521553161883 1109 4 -0.24869767448102564 1109 5 0.08837727026735465 1109 6 -0.5164950366975134 1109 7 -1.2404834916200542 1109 8 -0.5294337212876243 1109 9 -0.310495603908592 1109 10-11 0.3535924218952715 1109 12-13 -0.7778979491461868 1109 14-15 0.28122440902409807 1109 16-17 -1.0538346390883575 1109 18-19 -0.07739248898344897 1109 20-21 0.03545686937953363 1109 22-23 0.7526376620182127 1109 24-25 0.8155184764064671 1109 26-27 0.6444128003457799 1109 28-29 0.1692978316955127 1109 30-31 0.5159399790617947 1109 32-33 0.7275114443164696 1109 34-35 -0.31134845363378716 1109 36-37 -0.39079072260991055 1109 38-39 0.2889693197840799 1109 40-41 0.01304675595889293 1109 42-43 0.6618311325077748 1109 44-45 -0.5804168604624174 1109 46-47 -0.38392181183738927 1109 48-49 -0.046093487800252575 1109 50-51 0.6160158429230549 1109 52-53 0.7864385302983408 1109 54-55 0.6492427667664344 1109 56-57 0.02440855011475307 1109 58-59 -0.08122301865468629 1109 60-61 0.024088021428774198 1109 62-63 -0.6963293380699334 1109 64-65 -0.11113058953317534 1109 66-67 0.01798590669502076 1109 68-69 0.0774809385936841 1109 70-71 0.03311692648778575 1109 72-73 -0.8288744277018125 1109 74-75 0.7994270261497824 1109 76 -0.33907740193083313 1110 1 1.4162622397789768 1110 2 1.312855764080787 1110 3 0.43427319232251804 1110 4 0.24822350778498503 1110 5 0.49129188438886473 1110 6 0.47303698300692076 1110 7 0.21210271527649738 1110 8 1.1054184954611443 1110 9 -1.0927205628576893 1110 10-11 -0.22850938434446277 1110 12-13 0.2485798833661299 1110 14-15 0.19922523004215975 1110 16-17 0.749428907709671 1110 18-19 1.532521304120003 1110 20-21 0.853396114042912 1110 22-23 0.9192017014270739 1110 24-25 0.5121523023506285 1110 26-27 -0.5329271011320564 1110 28-29 0.5810383899877927 1110 30-31 1.1554104224115527 1110 32-33 1.6571091454658955 1110 34-35 0.700248426497577 1110 36-37 0.4086756156495355 1110 38-39 1.2695727680599518 1110 40-41 0.7725910909342666 1110 42-43 1.3296266990102374 1110 44-45 2.2619476715572837 1110 46-47 1.0847768745337092 1110 48-49 1.5334426369944936 1110 50-51 1.044279389721087 1110 52-53 0.6347554432704285 1110 54-55 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-0.4878771870909411 2116 48-49 -0.8450004068897279 2116 50-51 -0.1417033367028182 2116 52-53 -0.45172915056711105 2116 54-55 -0.32314848858628054 2116 56-57 -0.23280353011964294 2116 58-59 -1.2801186688687949 2116 60-61 -2.311273709470484 2116 62-63 0.21323793711600203 2116 64-65 -0.048870053134709934 2116 66-67 -1.181191465403245 2116 68-69 -0.37164008912774804 2116 70-71 -2.7960469229600413 2116 72-73 -0.7232287211433253 2116 74-75 -1.176739302450624 2116 76 -1.2989602056270257 2117 1 0.5259930886402593 2117 2 -0.10381090258588088 2117 3 0.5225237099208613 2117 4 -0.11487173031025222 2117 5 -1.648718467578007 2117 6 -1.2989609465997063 2117 7 -3.6509925228187434 2117 8 -2.6148195997769506 2117 9 -0.04173712600468704 2117 10-11 -0.8149483077399076 2117 12-13 0.18827967632679687 2117 14-15 -1.0299414366245045 2117 16-17 0.0722808745833774 2117 18-19 -0.17102424453424447 2117 20-21 -0.8595956043835855 2117 22-23 0.16079072834218877 2117 24-25 0.45418128785787815 2117 26-27 0.5234911183296482 2117 28-29 0.3367319717683408 2117 30-31 -0.0848846086443551 2117 32-33 0.036767530559064454 2117 34-35 0.07770701863008256 2117 36-37 -1.02326020215585 2117 38-39 -0.002295761960755982 2117 40-41 -1.2164099442624234 2117 42-43 -1.7931735080291027 2117 44-45 -0.3363960137429238 2117 46-47 -0.3180440364393746 2117 48-49 -0.6519869696307659 2117 50-51 0.5464791826817468 2117 52-53 0.17279892153129595 2117 54-55 -1.3283041740488706 2117 56-57 -0.6637295237054808 2117 58-59 -0.5172193062251864 2117 60-61 1.1237524749163157 2117 62-63 0.07226850586524236 2117 64-65 -0.6786147046184894 2117 66-67 -1.3755851649120352 2117 68-69 -0.6513867704089584 2117 70-71 0.7739607902701806 2117 72-73 1.1270940166474404 2117 74-75 -0.05123692758763809 2117 76 -0.2835337451877784 2118 1 0.32618765572848574 2118 2 0.7576348805466502 2118 3 -0.394111776122692 2118 4 0.1889148445606601 2118 5 -0.5466455797534167 2118 6 0.7264076083654984 2118 7 0.43248424138091224 2118 8 -3.1225648665583634 2118 9 -0.7214497653820722 2118 10-11 -0.43791844917521416 2118 12-13 0.18152595336039568 2118 14-15 -0.2752082180358677 2118 16-17 0.13751697426159382 2118 18-19 -0.5957417480888374 2118 20-21 -1.9009785273799196 2118 22-23 -0.25352491934171084 2118 24-25 0.1350725547889553 2118 26-27 -0.40412618317451177 2118 28-29 0.3433734444915295 2118 30-31 -0.11814809738190846 2118 32-33 -0.3413848304377183 2118 34-35 0.7906457299972907 2118 36-37 0.7653164647602644 2118 38-39 -0.5092926937874438 2118 40-41 -0.6869255470462292 2118 42-43 -0.29347284201098844 2118 44-45 -0.36642295380129397 2118 46-47 -0.17013563263783738 2118 48-49 -0.5652736567519199 2118 50-51 0.31640360085132 2118 52-53 -0.30780049476744153 2118 54-55 0.9631535824429349 2118 56-57 0.8131843429412307 2118 58-59 0.884224980237434 2118 60-61 -1.7973356041078539 2118 62-63 0.4357160850016619 2118 64-65 -0.015402850992806805 2118 66-67 -1.5037562342826867 2118 68-69 0.7288796368349217 2118 70-71 -0.9797621761295794 2118 72-73 -2.143616153224592 2118 74-75 -0.06440762279081014 2118 76 -0.5547677495281214 2119 1 1.4786596125211737 2119 2 1.4823959939658415 2119 3 0.9936738491369681 2119 4 1.2398080726454133 2119 5 1.4366531323363212 2119 6 2.177757836865702 2119 7 0.41009122102362383 2119 8 2.3970030603215697 2119 9 0.9504239363212967 2119 10-11 2.005891272469981 2119 12-13 1.6675043497043838 2119 14-15 1.085822110173524 2119 16-17 0.6863952459691092 2119 18-19 0.8145615340050583 2119 20-21 -0.18178450993081086 2119 22-23 2.4917181217883204 2119 24-25 1.6735644534179528 2119 26-27 1.8472863635641694 2119 28-29 2.271021969626549 2119 30-31 2.0866296342342103 2119 32-33 0.9234884558107197 2119 34-35 1.923134304986906 2119 36-37 1.1919678980797492 2119 38-39 1.0929923082898299 2119 40-41 0.7038513536600455 2119 42-43 0.6348196382548998 2119 44-45 0.2977440590778109 2119 46-47 1.6407908318407678 2119 48-49 0.992412259326187 2119 50-51 2.2217629693598404 2119 52-53 -0.1037913547591316 2119 54-55 0.892920927685978 2119 56-57 1.4629142972411877 2119 58-59 2.669351693988993 2119 60-61 1.6643179064862466 2119 62-63 0.7042453745737483 2119 64-65 2.119329180581765 2119 66-67 1.6932732630168594 2119 68-69 1.1906993293982815 2119 70-71 0.31587554717744126 2119 72-73 -1.1053112093110045 2119 74-75 1.0080477158049526 2119 76 1.587359379678361 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 17.0 13 106.0 14 448.0 15 944.0 16 1416.0 17 1937.0 18 2026.0 19 1844.0 20 1609.0 21 1373.0 22 1023.0 23 853.0 24 794.0 25 762.0 26 875.0 27 991.0 28 1135.0 29 1419.0 30 1640.0 31 1993.0 32 2494.0 33 3317.0 34 4451.0 35 6593.0 36 10799.0 37 11089.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.46542721905041 21.75864929488118 9.839132061354283 33.93679142471413 2 17.20608887956222 21.545141971621817 31.740625353113046 29.508143795702917 3 15.373936625288545 24.340990169332837 27.889070041485738 32.39600316389288 4 11.356115514374727 24.38941710116386 34.255597346204134 29.998870038257273 5 10.661996158130075 40.184668033382295 31.824565368286816 17.32877044020081 6 22.03748244523721 36.97396245298552 16.624965697589953 24.363589404187316 7 21.80341894138727 30.518652439910248 20.332854444785227 27.345074173917254 8 19.35301619073754 34.891604384251565 21.259423073818787 24.49595635119211 9 20.87362185023164 19.72913202795848 20.56691794863517 38.83032817317471 10-11 20.714620090719784 26.36604303540009 27.381394372790517 25.537942501089606 12-13 20.815509532034415 24.428965762159198 25.748599654554553 29.006925051251837 14-15 20.37078887471953 25.832539669728327 23.23443477699398 30.56223667855817 16-17 21.236016723433792 28.17478893928877 24.50644885308883 26.082745484188607 18-19 19.659720092334016 26.930216791231498 25.191689938497795 28.21837317793669 20-21 19.101196145216225 27.738139437279052 25.28289399344622 27.8777704240585 22-23 21.713022001969364 27.174772796978157 24.966504705483544 26.145700495568935 24-25 20.38289560767728 29.148170269092315 24.482235387173322 25.98669873605708 26-27 19.03016997853073 29.495229947214646 25.981856042873975 25.492744031380653 28-29 20.64359392403429 27.872927730875396 27.352338213691908 24.13114013139841 30-31 20.845372806663544 27.394308221278795 27.339424365203634 24.420894606854024 32-33 19.038241133835896 29.78256307607871 26.06176048039516 25.11743530969023 34-35 19.665369901047637 28.90038580122359 26.24094012816995 25.193304169558832 36-37 20.266670971282828 27.54039613230238 26.642883662367435 25.55004923404736 38-39 20.025343427658235 27.048055658686987 27.723611357729744 25.202989555925036 40-41 19.608871813911442 26.31842321909958 27.714733086894057 26.357971880094915 42-43 18.221440216952654 26.216726662254437 28.634037676152964 26.927795444639948 44-45 18.128621930943194 26.720366753297064 28.889086183796348 26.261925131963388 46-47 18.763821853460104 27.244991848133143 28.24016529726065 25.7510210011461 48-49 18.08100211464269 28.223215871119795 27.397536683400865 26.298245330836657 50-51 17.918771893008763 27.658234999757862 26.742158872621026 27.680834234612345 52-53 17.286800432613923 27.10213239923163 27.938304088847275 27.672763079307174 54-55 17.36751198566563 27.688098274386995 27.173965681447644 27.77042405849973 56-57 17.86308092140309 27.569452291400992 26.90842467190754 27.659042115288386 58-59 18.422411984051397 27.178001259100228 26.500024213465917 27.899562543382462 60-61 18.006747485835124 26.290174175531483 26.71068136693086 28.992396971702526 62-63 18.07293095933752 27.14087394469644 26.22883339521219 28.557361700753848 64-65 18.662125296614956 27.377358795137933 24.940677008507 29.019838899740108 66-67 18.455503720802596 28.15864662867843 24.743740819060843 28.642108831458135 68-69 18.744451080727696 28.05130026311966 24.753426205427047 28.4508224507256 70-71 19.876027054512583 28.214337600284107 24.491920773539526 27.417714571663787 72-73 20.37805291449418 27.756703094480944 24.40313806518265 27.462105925842224 74-75 19.940596296953945 28.386253208284234 23.955188945745697 27.717961549016124 76 19.742045876446753 27.50972574214273 25.140034544544704 27.60819383686581 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 1.0 12 2.0 13 3.0 14 4.5 15 4.0 16 7.0 17 11.0 18 10.5 19 15.5 20 19.0 21 17.0 22 32.0 23 53.0 24 62.0 25 65.0 26 119.5 27 207.5 28 263.5 29 286.0 30 367.5 31 510.0 32 678.5 33 786.0 34 900.0 35 1199.0 36 1517.5 37 1651.0 38 1899.0 39 2492.0 40 3117.5 41 3534.5 42 3671.0 43 3841.0 44 4178.0 45 4458.5 46 4572.0 47 4572.0 48 4465.5 49 4160.0 50 3961.0 51 3739.0 52 3248.5 53 2673.5 54 2367.0 55 2230.0 56 1873.5 57 1459.0 58 1264.0 59 1100.0 60 805.5 61 604.0 62 533.0 63 442.5 64 294.5 65 203.0 66 148.5 67 128.0 68 119.5 69 79.5 70 43.5 71 39.0 72 33.0 73 27.0 74 23.0 75 19.0 76 17.5 77 15.5 78 12.0 79 9.0 80 8.5 81 7.5 82 6.0 83 5.0 84 4.5 85 4.5 86 4.5 87 4.0 88 3.0 89 2.5 90 4.5 91 4.5 92 3.0 93 2.0 94 0.5 95 0.5 96 1.0 97 1.0 98 2.0 99 4.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7586885986860159 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 61949.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.74769568516037 #Duplication Level Percentage of deduplicated Percentage of total 1 82.47778189224218 57.52635232207138 2 8.570172190335123 11.95499523801837 3 3.635900759118682 7.607870990653602 4 1.8144788002221812 5.062228607402864 5 1.1849657470838735 4.132431516247236 6 0.7521755230512868 3.147750569016449 7 0.4860211072023699 2.3729196597200923 8 0.31475652656915387 1.756283394405075 9 0.20598037400481392 1.292999079888295 >10 0.5577670801703388 5.146168622576636 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006456924244136306 0.0 2 0.0 0.0 0.0 0.006456924244136306 0.0 3 0.0 0.0 0.0 0.006456924244136306 0.0 4 0.0 0.0 0.0 0.006456924244136306 0.0 5 0.0 0.0 0.0 0.006456924244136306 0.0 6 0.0 0.0 0.0 0.006456924244136306 0.0 7 0.0 0.0 0.0 0.006456924244136306 0.0 8 0.0 0.0 0.0 0.006456924244136306 0.0 9 0.0 0.0 0.0 0.006456924244136306 0.0 10 0.0 0.0 0.0 0.011299617427238534 0.0 11 0.0 0.0 0.0 0.012913848488272612 0.0 12 0.0 0.0 0.0 0.012913848488272612 0.0 13 0.0 0.0 0.0 0.014528079549306688 0.0 14 0.0 0.0 0.0 0.017756541671374842 0.0 15 0.0 0.0 0.0 0.017756541671374842 0.0 16 0.0 0.0 0.0 0.019370772732408916 0.0 17 0.0 0.0 0.0 0.022599234854477068 0.0 18 0.0 0.0 0.0 0.025827696976545224 0.0 19 0.0 0.0 0.0 0.029056159098613376 0.0 20 0.0 0.0 0.0 0.029056159098613376 0.0 21 0.0 0.0 0.0 0.03874154546481783 0.0 22 0.0 0.0 0.0 0.045198469708954137 0.0 23 0.0 0.0 0.0 0.045198469708954137 0.0 24 0.0 0.0 0.0 0.05165539395309045 0.0 25 0.0 0.0 0.0 0.054883856075158596 0.0 26 0.0 0.0 0.0 0.05649808713619268 0.0 27 0.0 0.0 0.0 0.07264039774653344 0.0 28 0.0 0.0 0.0 0.08555424623480605 0.0 29 0.0 0.0 0.0 0.10976771215031719 0.0 30 0.0 0.0 0.0 0.1291384848827261 0.0 31 0.0 0.0 0.0 0.14689502655410094 0.0 32 0.0 0.0 0.0 0.19532195838512326 0.0 33 0.0 0.0 0.0 0.22922081066683886 0.0 34 0.0 0.0 0.0 0.29217582204716785 0.0 35 0.0 0.0 0.0 0.3486739091833605 0.0 36 0.0 0.0 0.0 0.4794266251271207 0.0 37 0.0 0.0 0.0 0.6666774282070735 0.0 38 0.0 0.0 0.0 0.9749955608645822 0.0 39 0.0 0.0 0.0 1.349497167024488 0.0 40 0.0 0.0 0.0 1.7207703110623254 0.0 41 0.0 0.0 0.0 2.1840546255791056 0.0 42 0.0 0.0 0.0 2.6957658719269078 0.0 43 0.0 0.0 0.0 3.150979031138517 0.0 44 0.0 0.0 0.0 3.6691472017304556 0.0 45 0.0 0.0 0.0 4.1889296033834285 0.0 46 0.0 0.0 0.0 4.634457376228833 0.0 47 0.0 0.0 0.0 5.213966327140067 0.0 48 0.0 0.0 0.0 5.772490274257858 0.0 49 0.0 0.0 0.0 6.229317664530501 0.0 50 0.0 0.0 0.0 6.715201213901758 0.0 51 0.0 0.0 0.0 7.26242554359231 0.0 52 0.0 0.0 0.0 7.787050638428385 0.0 53 0.0 0.0 0.0 8.36333112721755 0.0 54 0.0 0.0 0.0 8.84598621446674 0.0 55 0.0 0.0 0.0 9.339940919143165 0.0 56 0.0 0.0 0.0 9.814524851087185 0.0 57 0.0 0.0 0.0 10.230996464833977 0.0 58 0.0 0.0 0.0 10.650696540702835 0.0 59 0.0 0.0 0.0 11.075239309754798 0.0 60 0.0 0.0 0.0 11.53529516214951 0.0 61 0.0 0.0 0.0 11.921096385736654 0.0 62 0.0 0.0 0.0 12.321425688873106 0.0 63 0.0 0.0 0.0 12.816994624610567 0.0 64 0.0 0.0 0.0 13.254451242150802 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGTCG 15 0.002215554 69.995155 18 AGAGTGT 15 0.002215554 69.995155 65 ATATAGA 15 0.002215554 69.995155 6 TACGAGT 15 0.002215554 69.995155 4 GTACTGG 15 0.002215554 69.995155 38 GCACTTG 15 0.002215554 69.995155 50 AGTCGTA 15 0.002215554 69.995155 20 CACTGCT 25 2.3741579E-4 55.996124 42 ATATTAG 20 0.006791157 52.75203 1 TTGGCGG 20 0.0069230543 52.496365 8 GCGTTGG 20 0.0069230543 52.496365 7 GTTACTA 20 0.0069230543 52.496365 19 AACATGG 20 0.0069230543 52.496365 26 ACCAGAG 20 0.0069230543 52.496365 62 TCGTAAC 20 0.0069230543 52.496365 22 ACAAGTC 20 0.0069230543 52.496365 17 CACTTGG 20 0.0069230543 52.496365 51 TGGTAAT 20 0.0069230543 52.496365 9 AAGTCGT 20 0.0069230543 52.496365 19 GTGTACT 30 5.8381533E-4 46.663437 36 >>END_MODULE