FastQCFastQC Report
Wed 25 May 2016
SRR1780506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61949
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT28164.5456746678719595No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT25844.171173061712054No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC9531.5383622011654747No Hit
CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC4050.653763579718801No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG2570.4148573826857576No Hit
TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT2100.3389885228171561No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT1490.2405204280940774No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1360.21953542430063439No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT1220.19693618944615732No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1190.1920934962630551No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT1100.1775654167137484No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG1100.1775654167137484No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT1060.1711084924696121No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG960.15496618185927133No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT880.14205233337099873No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTCTT860.13882387124893059No Hit
CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC770.12429579169962388No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC650.10492501896721496No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCT150.002214945670.0000135
CTATGCG150.002214945670.0000141
GATGTAT150.002214945670.0000124
ATCTAGT150.002214945670.0000136
CGTGCGA150.002214945670.0000110
TTTGTTT150.002214945670.0000133
GCTATGC150.002214945670.0000140
GGATCTA150.002214945670.0000134
TATGCGA150.002214945670.0000142
TTGAGTG150.002214945670.0000143
CCCGTGC150.002214945670.000018
GAGGTGC150.002214945670.0000135
CTTTGTT150.002214945670.0000132
TTGCGGC150.002214945670.0000124
TTACGTT150.002214945670.0000170
CGAGAAT150.002214945670.0000114
GCCGTAT150.002214945670.0000146
TTTACAC1350.062.222222
TAATGAT252.373346E-455.99999620
GCGAGAA200.0069211652.513
Produced by FastQC (version 0.11.5)