##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780506_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61949 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.00656992041841 34.0 34.0 34.0 32.0 34.0 2 33.35811716089041 34.0 34.0 34.0 33.0 34.0 3 33.50866034964245 34.0 34.0 34.0 34.0 34.0 4 33.529871345784436 34.0 34.0 34.0 34.0 34.0 5 33.52857996093561 34.0 34.0 34.0 34.0 34.0 6 37.28011751602124 38.0 38.0 38.0 36.0 38.0 7 36.9080533987635 38.0 37.0 38.0 35.0 38.0 8 36.96524560525594 38.0 37.0 38.0 35.0 38.0 9 36.737848875688066 38.0 37.0 38.0 34.0 38.0 10-11 36.45806227703433 38.0 37.0 38.0 34.0 38.0 12-13 36.30477489547854 38.0 37.0 38.0 34.0 38.0 14-15 36.49765936496151 38.0 37.0 38.0 34.0 38.0 16-17 36.57904889505884 38.0 37.0 38.0 34.0 38.0 18-19 36.63123698526207 38.0 37.0 38.0 34.0 38.0 20-21 36.38719753345494 38.0 37.0 38.0 34.0 38.0 22-23 36.44769084246719 38.0 37.0 38.0 34.0 38.0 24-25 35.690785969103615 38.0 36.5 38.0 31.5 38.0 26-27 35.881394372790524 38.0 37.0 38.0 31.5 38.0 28-29 35.73730810828262 38.0 36.5 38.0 31.5 38.0 30-31 35.47737655167961 38.0 36.0 38.0 30.5 38.0 32-33 35.91813427173966 38.0 36.5 38.0 33.0 38.0 34-35 35.652399554472225 38.0 36.5 38.0 31.0 38.0 36-37 35.6995028168332 38.0 36.0 38.0 31.0 38.0 38-39 35.12371466851765 38.0 35.5 38.0 29.0 38.0 40-41 35.18554778931056 37.0 35.5 38.0 29.0 38.0 42-43 35.82772926116644 38.0 36.5 38.0 32.5 38.0 44-45 36.02739350110575 38.0 37.0 38.0 33.0 38.0 46-47 36.243595538265346 38.0 37.0 38.0 34.0 38.0 48-49 35.98518943001501 38.0 37.0 38.0 32.5 38.0 50-51 36.109138162036515 38.0 37.0 38.0 34.0 38.0 52-53 35.99977400765145 38.0 37.0 38.0 33.5 38.0 54-55 36.193877221585495 38.0 37.0 38.0 34.0 38.0 56-57 36.20849408384316 38.0 37.0 38.0 34.0 38.0 58-59 36.205741819884096 38.0 37.0 38.0 34.0 38.0 60-61 35.95870796945875 38.0 37.0 38.0 32.5 38.0 62-63 36.30582414566821 38.0 37.0 38.0 34.0 38.0 64-65 36.27293418779964 38.0 37.0 38.0 34.0 38.0 66-67 35.383743078984324 38.0 36.0 38.0 31.0 38.0 68-69 34.10088944131463 37.5 34.0 38.0 23.5 38.0 70-71 33.369150430192576 37.0 34.0 38.0 20.5 38.0 72-73 32.514229446803014 37.0 31.5 38.0 11.0 38.0 74-75 32.865252062180176 37.5 33.0 38.0 11.0 38.0 76 32.535117596732796 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3635831592273959 1101 2 0.34854069586389613 1101 3 0.0326397007485042 1101 4 0.06325220715738311 1101 5 0.10801653275974843 1101 6 -1.791290895791363 1101 7 -0.08107300877831136 1101 8 0.15830861319077627 1101 9 0.03675866383426296 1101 10-11 -0.046827676182566336 1101 12-13 0.4523991908468048 1101 14-15 0.533739421801215 1101 16-17 -0.4278198740399688 1101 18-19 -0.5481626583248058 1101 20-21 -0.3468803867710477 1101 22-23 0.6210339273798269 1101 24-25 -1.1116529850519115 1101 26-27 -0.4918023640753404 1101 28-29 0.6506787840349091 1101 30-31 0.6850855397118423 1101 32-33 -0.14413506490453898 1101 34-35 0.3888650984130777 1101 36-37 0.28923316567556157 1101 38-39 -0.036785432280581176 1101 40-41 0.9449784296037009 1101 42-43 0.41658130254283776 1101 44-45 0.5777524188751215 1101 46-47 0.6521906273806053 1101 48-49 -1.1684980416429838 1101 50-51 -1.3319242680092813 1101 52-53 -1.8499162782549021 1101 54-55 -1.1144163316986067 1101 56-57 -0.9953595448466643 1101 58-59 -1.402832281376483 1101 60-61 -1.3441533947789992 1101 62-63 -0.13154686138276617 1101 64-65 -1.3592278164720923 1101 66-67 -2.157621414940749 1101 68-69 -1.114500356019768 1101 70-71 -1.9167909989147418 1101 72-73 -3.6082125366230606 1101 74-75 -2.3106858682565274 1101 76 -2.4926825969874997 1102 1 -0.18722329238551083 1102 2 0.09951692676372659 1102 3 0.18085701823576983 1102 4 0.38443217320152456 1102 5 0.23259208454242497 1102 6 0.3560350804395398 1102 7 0.07338369750352314 1102 8 0.47702677957448003 1102 9 0.47916104074428034 1102 10-11 0.5527266531892465 1102 12-13 0.5165120940726098 1102 14-15 -0.5348301877064259 1102 16-17 0.24210796976308302 1102 18-19 -0.7444487364232799 1102 20-21 0.430645080121991 1102 22-23 0.559998273729569 1102 24-25 -4.279034139551058 1102 26-27 -0.9537930262145551 1102 28-29 0.11897250220129507 1102 30-31 1.5282307010021654 1102 32-33 0.3872104359443611 1102 34-35 0.5553761340667265 1102 36-37 -2.15877192770305 1102 38-39 0.01813816704029847 1102 40-41 1.0107891256987713 1102 42-43 0.5864030342915569 1102 44-45 0.770515576769867 1102 46-47 0.9616346002159162 1102 48-49 -4.216036241982543 1102 50-51 -0.9990719759889046 1102 52-53 -0.1227303699357094 1102 54-55 0.295070085958443 1102 56-57 0.8574630357985029 1102 58-59 0.10973138585611508 1102 60-61 -5.207629625678834 1102 62-63 -2.263486589735905 1102 64-65 0.23013975568410672 1102 66-67 1.4095899602714752 1102 68-69 -0.46874483819294 1102 70-71 -1.2327502518858822 1102 72-73 -5.2839340985925 1102 74-75 -1.5386357833668818 1102 76 0.09224949129772853 1103 1 0.4490126626706683 1103 2 0.4975802029316725 1103 3 0.09599598925883868 1103 4 0.3219691335799979 1103 5 0.2156642312012096 1103 6 0.41273230101907643 1103 7 0.41334525870991 1103 8 0.2514945501244483 1103 9 0.6029783086331051 1103 10-11 0.5195731936978305 1103 12-13 -0.03475195087121108 1103 14-15 -0.29262882756449216 1103 16-17 0.19845776278496885 1103 18-19 0.6582345870539541 1103 20-21 -0.2424403722730375 1103 22-23 0.30807633404890566 1103 24-25 -2.6928055765705707 1103 26-27 -0.41479982692419526 1103 28-29 0.33016410480330194 1103 30-31 0.9432587908898071 1103 32-33 1.432486899575352 1103 34-35 0.10024603353449635 1103 36-37 -0.7037896686847134 1103 38-39 0.01843385006809939 1103 40-41 0.2435286289512888 1103 42-43 -0.062251607931976594 1103 44-45 1.2137074750549033 1103 46-47 1.1019731572827425 1103 48-49 -1.923753569007971 1103 50-51 -0.23014826221007212 1103 52-53 0.410866614097408 1103 54-55 -0.09674688624735239 1103 56-57 0.4241765189445701 1103 58-59 -0.07403561591797825 1103 60-61 -4.0993726771276755 1103 62-63 -1.2686980031007806 1103 64-65 0.40588635532928663 1103 66-67 0.05872508741518345 1103 68-69 0.33107775199038514 1103 70-71 0.9167175186640932 1103 72-73 -3.1488069548905493 1103 74-75 -1.2827590831894717 1103 76 -1.9644477292818117 1104 1 -0.2863612234199948 1104 2 -0.15494465823324788 1104 3 0.24135308599137062 1104 4 -0.4914752678148133 1104 5 -0.6750757920455968 1104 6 -0.29603690988108866 1104 7 0.11036191891912495 1104 8 -0.007319862893758966 1104 9 0.5814750153359327 1104 10-11 -1.0363159965385123 1104 12-13 0.22700059981973197 1104 14-15 0.31540423455613364 1104 16-17 -0.1524398221304395 1104 18-19 0.4257342641816848 1104 20-21 -0.4742626633746809 1104 22-23 -0.22040100031762222 1104 24-25 -0.05342161686503033 1104 26-27 0.22319578201290824 1104 28-29 0.9036821210760735 1104 30-31 0.3910180573240041 1104 32-33 0.36252047829160716 1104 34-35 0.15920251035226585 1104 36-37 0.7065487014560574 1104 38-39 0.9217981791395147 1104 40-41 0.872064080326723 1104 42-43 -0.47507609456506117 1104 44-45 0.27947655680615924 1104 46-47 0.582891406023542 1104 48-49 -0.357597040530635 1104 50-51 -0.17632545451279924 1104 52-53 -0.20837011585222598 1104 54-55 -0.4278201136785782 1104 56-57 -0.018255355006090213 1104 58-59 -1.3095063636901614 1104 60-61 -0.30733470734852375 1104 62-63 -0.47820982022592773 1104 64-65 0.32833243565990955 1104 66-67 -0.06012419580837047 1104 68-69 1.0522775453521334 1104 70-71 0.5084778182895562 1104 72-73 0.0363925801733771 1104 74-75 0.12670753181762962 1104 76 -0.531540587347159 1105 1 0.5775616538510491 1105 2 0.3689708033907735 1105 3 0.21651066849044298 1105 4 -0.05932843800390941 1105 5 -0.3220909941219716 1105 6 0.23881663109035856 1105 7 0.8189269912216872 1105 8 -0.21588493519632124 1105 9 0.5303070509310359 1105 10-11 0.3365056571507665 1105 12-13 -0.07286962635750172 1105 14-15 0.1047071637366983 1105 16-17 0.21787905069120939 1105 18-19 0.28140723414831825 1105 20-21 0.6429045594655065 1105 22-23 0.5092059703905747 1105 24-25 0.5598523912921749 1105 26-27 0.22217613054831986 1105 28-29 0.7275605044650177 1105 30-31 0.9845479053032378 1105 32-33 1.0338219243427815 1105 34-35 -0.3541456542750936 1105 36-37 0.015039617288465479 1105 38-39 0.23149413761188953 1105 40-41 0.39551606401229833 1105 42-43 0.6375490444783196 1105 44-45 0.851408332853623 1105 46-47 0.8258465413590983 1105 48-49 -0.319035676051584 1105 50-51 0.7659251943563135 1105 52-53 0.3463202808848891 1105 54-55 0.8694546360433293 1105 56-57 0.5207694874114068 1105 58-59 -0.2598215286883061 1105 60-61 0.4026207987693837 1105 62-63 -0.6320844957913678 1105 64-65 0.4327076673988799 1105 66-67 0.6413033162420518 1105 68-69 0.027435127851198615 1105 70-71 0.7235315817304198 1105 72-73 0.24124982896833558 1105 74-75 -0.5891804919124368 1105 76 0.053553962152285806 1106 1 -1.2925263226885413 1106 2 0.226351057545223 1106 3 0.38356834199972667 1106 4 0.3113460487996065 1106 5 0.17800675758868323 1106 6 0.2775263085097137 1106 7 -0.8644552179669773 1106 8 0.25840636490143254 1106 9 -0.778295099606602 1106 10-11 0.37575296897872335 1106 12-13 1.3191636092241197 1106 14-15 0.1685390307943706 1106 16-17 0.19886634296882733 1106 18-19 0.6454834804826248 1106 20-21 0.29485959367860914 1106 22-23 0.8771434092957549 1106 24-25 -0.44312903588279795 1106 26-27 0.8642093661299413 1106 28-29 0.7601606999684378 1106 30-31 0.865730701002164 1106 32-33 0.6676928920847089 1106 34-35 1.215942322264496 1106 36-37 1.4820972907977463 1106 38-39 0.6167825051583193 1106 40-41 0.6651152819986166 1106 42-43 0.8940984090922015 1106 44-45 0.7898736309963397 1106 46-47 1.0888475188762072 1106 48-49 1.4734374422279828 1106 50-51 0.2611353605342188 1106 52-53 0.49690679114882386 1106 54-55 0.10190820398076994 1106 56-57 0.35556909640456524 1106 58-59 0.6939419121719155 1106 60-61 0.9174301829336073 1106 62-63 0.8412781630551649 1106 64-65 -0.8417302602648604 1106 66-67 0.8309416349126337 1106 68-69 2.3793412862089767 1106 70-71 1.4615081213198593 1106 72-73 1.12834660316188 1106 74-75 0.8690795276379006 1106 76 0.29500068746997954 1107 1 0.7974258304215098 1107 2 -0.010863190195337324 1107 3 0.2071728077094548 1107 4 0.3690812960085381 1107 5 0.16460962840207571 1107 6 0.2997485307319394 1107 7 0.6954597208953359 1107 8 0.0625530953639526 1107 9 -0.4835582575013362 1107 10-11 0.5669810391541645 1107 12-13 0.08120507652874664 1107 14-15 0.19214349650410156 1107 16-17 0.8660114785350146 1107 18-19 0.6864723162082953 1107 20-21 0.7321801678412854 1107 22-23 -0.2397093286096137 1107 24-25 0.17893369670625248 1107 26-27 0.32800521939947913 1107 28-29 1.2951420928445714 1107 30-31 0.7581283618208801 1107 32-33 1.1185700850671694 1107 34-35 0.11312467207842047 1107 36-37 1.2630116980279453 1107 38-39 0.42895688049059544 1107 40-41 0.7918563771607623 1107 42-43 0.6280697009582283 1107 44-45 1.1268752258926753 1107 46-47 1.2748655943679665 1107 48-49 0.9594023545086827 1107 50-51 0.7626239304438442 1107 52-53 0.9463485774327083 1107 54-55 0.6353478637362144 1107 56-57 0.8739104042195578 1107 58-59 0.7471582864409143 1107 60-61 1.4228528304615153 1107 62-63 1.000767796228999 1107 64-65 1.101119287847844 1107 66-67 0.6124408374644617 1107 68-69 1.3280387875380555 1107 70-71 0.16783454343575954 1107 72-73 0.5844869540390789 1107 74-75 -0.41619426289904027 1107 76 0.11637229831526952 1108 1 -0.2018880968547876 1108 2 0.5150932278516365 1108 3 0.22128630374003677 1108 4 0.39534808028767543 1108 5 0.26173792400140883 1108 6 -0.28932657789624017 1108 7 -0.18062247715099033 1108 8 -0.5599108858180557 1108 9 -0.15296921506283923 1108 10-11 0.45564123713515414 1108 12-13 1.0140679599943923 1108 14-15 0.33474861264641476 1108 16-17 0.06864795800183998 1108 18-19 0.4528645537854885 1108 20-21 0.9884773353410452 1108 22-23 -0.21293615732012228 1108 24-25 0.6092156399255586 1108 26-27 -0.6298993184815345 1108 28-29 -0.9076740723356025 1108 30-31 0.13612176245467822 1108 32-33 0.6345457784906685 1108 34-35 0.6878739288329783 1108 36-37 1.3298711184598417 1108 38-39 1.240940284064699 1108 40-41 0.6794711309913382 1108 42-43 -0.5024311321300772 1108 44-45 0.7850149887075304 1108 46-47 0.6003254264435043 1108 48-49 1.199135766250329 1108 50-51 0.6192020610590205 1108 52-53 -0.11392781186455636 1108 54-55 -0.4241117723185326 1108 56-57 0.30892951624542775 1108 58-59 0.8386346496020778 1108 60-61 0.9406907212779458 1108 62-63 0.9323565446363347 1108 64-65 0.8241745983774322 1108 66-67 0.9667973992600096 1108 68-69 1.498691210028177 1108 70-71 -0.43798400666388204 1108 72-73 -0.3167185737468685 1108 74-75 -0.561037282925831 1108 76 0.4107955071754077 1109 1 -1.3725681199717172 1109 2 0.13815879004260978 1109 3 0.3103186032327443 1109 4 -0.02595802643550371 1109 5 -0.17507579204559676 1109 6 -0.37649667999603054 1109 7 -0.3494081960234041 1109 8 0.29153071181887924 1109 9 0.6504405325773064 1109 10-11 0.2452931988637843 1109 12-13 0.7155063469461709 1109 14-15 0.7320709012227979 1109 16-17 0.7469854652258832 1109 18-19 0.2102170228023681 1109 20-21 0.9280361872000285 1109 22-23 0.11005876979731966 1109 24-25 -0.12238713410641111 1109 26-27 0.37836819580601144 1109 28-29 -0.8175822467400167 1109 30-31 0.3249261033010171 1109 32-33 0.6786124323146012 1109 34-35 -0.19999289194658587 1109 36-37 1.1260889313411155 1109 38-39 0.9649016274153794 1109 40-41 0.5358571837750006 1109 42-43 0.8237744801475841 1109 44-45 0.47200529243834666 1109 46-47 0.9478339347591742 1109 48-49 0.5533224996992487 1109 50-51 0.30068603974007857 1109 52-53 -0.1997494261970516 1109 54-55 -0.11747528609237179 1109 56-57 0.1512848748789679 1109 58-59 -0.010655788977516067 1109 60-61 1.1409411547204442 1109 62-63 0.5217901797740723 1109 64-65 0.8944243896828965 1109 66-67 0.2588413214330032 1109 68-69 -0.5195615351076412 1109 70-71 -0.2156601127449207 1109 72-73 -0.5153315577576549 1109 74-75 -0.32156833025133835 1109 76 0.968459412652841 1110 1 -0.7348423400045618 1110 2 -0.5163978602036963 1110 3 -0.2925139090825226 1110 4 -0.07296131050775045 1110 5 0.3378552424371648 1110 6 0.18466916565257208 1110 7 0.5455014919897323 1110 8 0.5873024687975317 1110 9 0.8657525194410169 1110 10-11 -0.07603274530698911 1110 12-13 0.4718692369297486 1110 14-15 0.8263853511406936 1110 16-17 0.5607169923194846 1110 18-19 -0.23395374895460463 1110 20-21 0.687067385886408 1110 22-23 -0.7210167638644123 1110 24-25 -0.7262459189996804 1110 26-27 1.1538197557403294 1110 28-29 0.7482018255095682 1110 30-31 -0.025340727569265198 1110 32-33 0.6813833682751849 1110 34-35 0.8987345300567071 1110 36-37 1.642919801620259 1110 38-39 1.1119664878423023 1110 40-41 -0.8983587439754146 1110 42-43 -0.08057691558312996 1110 44-45 0.8289428950656017 1110 46-47 0.8546483846770769 1110 48-49 1.4127231565136924 1110 50-51 -0.09822611132725001 1110 52-53 0.7137357954778238 1110 54-55 0.025176602249167956 1110 56-57 0.6759154167508825 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44-45 -0.36865280988304505 2116 46-47 -0.6472190289550497 2116 48-49 -0.06054948587659226 2116 50-51 -0.9023227499827087 2116 52-53 -0.28223759387792313 2116 54-55 -0.4924471139039639 2116 56-57 0.3635904867788895 2116 58-59 -1.9250123361941078 2116 60-61 -2.009723708926167 2116 62-63 0.5011117934770297 2116 64-65 -1.893720753431822 2116 66-67 -0.5728016805953899 2116 68-69 -0.904675350748839 2116 70-71 0.24540592286472673 2116 72-73 -0.4860324818054309 2116 74-75 -0.5030535680246047 2116 76 -1.5213985995175463 2117 1 0.4719381982977211 2117 2 0.3529085580157698 2117 3 0.18755855468559446 2117 4 -0.48108760450360677 2117 5 0.07155089461107877 2117 6 0.09201906213290556 2117 7 0.5589590489255514 2117 8 0.38880350399422525 2117 9 -0.3824358863560846 2117 10-11 -0.37145199996537315 2117 12-13 -0.37052206741807225 2117 14-15 0.3526534670827246 2117 16-17 -1.189671206438291 2117 18-19 -0.8731877034059536 2117 20-21 -0.08829493295418445 2117 22-23 0.32634348458278595 2117 24-25 -1.9074053392895323 2117 26-27 -1.5790994989180547 2117 28-29 -1.1248571807016106 2117 30-31 -0.5187413486872785 2117 32-33 -2.780961352221702 2117 34-35 -0.003180583814931026 2117 36-37 -1.2747820617337737 2117 38-39 0.07689920005762474 2117 40-41 0.2213358730018129 2117 42-43 0.09902970967566915 2117 44-45 -0.6247320531745615 2117 46-47 -0.860672526296014 2117 48-49 -1.4234569677099032 2117 50-51 -0.2833969187806602 2117 52-53 0.4944034973411746 2117 54-55 -0.30686273522495355 2117 56-57 0.30427980598483373 2117 58-59 -0.8675487710579048 2117 60-61 -0.7354777786700097 2117 62-63 -0.22403333703894646 2117 64-65 -0.7888411204191996 2117 66-67 -0.054716185527802 2117 68-69 0.15376251715194655 2117 70-71 -0.36367539082016975 2117 72-73 0.45029277293414083 2117 74-75 -0.2808922397251692 2117 76 0.14197979531864746 2118 1 -0.014030521301172882 2118 2 0.25289359131667055 2118 3 -0.5553509716183811 2118 4 0.2158367388324649 2118 5 0.2459877725576476 2118 6 0.18997610770649942 2118 7 0.7798675275605902 2118 8 0.6605239260551343 2118 9 -1.9586163847471596 2118 10-11 -0.5748494406598255 2118 12-13 -0.7839397131563075 2118 14-15 0.7338365846461485 2118 16-17 -2.8507503054430003 2118 18-19 -0.23553148848415617 2118 20-21 -0.21841167320711463 2118 22-23 0.6161174874264645 2118 24-25 0.7244138629854007 2118 26-27 -2.6585726711099227 2118 28-29 -0.7067250247486072 2118 30-31 -0.5011367688773518 2118 32-33 0.1431949001168462 2118 34-35 0.5868441112385767 2118 36-37 -2.558026842097469 2118 38-39 0.31997710172641547 2118 40-41 -0.23179965045848405 2118 42-43 0.2781436810892828 2118 44-45 -1.8716451714863211 2118 46-47 0.18353536115441216 2118 48-49 0.2842808157219565 2118 50-51 -0.48258497535478995 2118 52-53 -0.9369741873104758 2118 54-55 -0.46313378214555456 2118 56-57 0.3070112285695856 2118 58-59 -0.3656966420449592 2118 60-61 -0.8948918324004538 2118 62-63 -1.225717090687084 2118 64-65 0.3457469211882298 2118 66-67 0.39583758233869304 2118 68-69 -1.0305710905708665 2118 70-71 0.40707219579959997 2118 72-73 1.6715192939249377 2118 74-75 0.3060455736400911 2118 76 0.6077060543922101 2119 1 0.6274318800282828 2119 2 -1.022760750187274 2119 3 0.06089331587642732 2119 4 -0.7500959574699806 2119 5 -0.10955855066628573 2119 6 -0.46960012827189246 2119 7 -0.39653463280500745 2119 8 -0.14180262151445078 2119 9 -0.7874905019054523 2119 10-11 -0.9932125482626546 2119 12-13 -0.7511603197204906 2119 14-15 -0.32310151257030384 2119 16-17 -0.5374972933948072 2119 18-19 -0.2616220576573909 2119 20-21 -0.959894847282726 2119 22-23 -0.19109065549002935 2119 24-25 0.7793370038246223 2119 26-27 0.30652911534624394 2119 28-29 0.05713039693813471 2119 30-31 0.6622824251400985 2119 32-33 -0.1300082573405703 2119 34-35 0.5373634298925012 2119 36-37 0.1042498508813452 2119 38-39 -1.332224809366231 2119 40-41 -1.0537979886387987 2119 42-43 0.46975149164183705 2119 44-45 0.09154552232340052 2119 46-47 -0.575729283631631 2119 48-49 -0.34725221294442576 2119 50-51 0.5001113270963984 2119 52-53 0.24852643587191636 2119 54-55 0.24286954149383178 2119 56-57 -1.2257266193739085 2119 58-59 -0.13019601886256993 2119 60-61 0.30645839609974956 2119 62-63 -0.1351063719500658 2119 64-65 -0.18948365629411512 2119 66-67 0.8967723559157577 2119 68-69 1.3890591545475317 2119 70-71 0.7320410366803642 2119 72-73 1.9812201663802753 2119 74-75 0.8330293708980889 2119 76 1.6454709069057145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 5.0 22 12.0 23 11.0 24 45.0 25 91.0 26 159.0 27 299.0 28 491.0 29 848.0 30 1347.0 31 2025.0 32 2961.0 33 4476.0 34 6556.0 35 8311.0 36 11992.0 37 22319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.64057531195016 22.72353064617669 11.256033188590616 32.37986085328254 2 16.55232529984342 18.279552535149882 44.26544415567644 20.902678009330256 3 15.515988958659543 26.891475245766678 36.037708437585756 21.55482735798802 4 10.912201972590356 23.199728809181746 36.644659316534565 29.24340990169333 5 10.140599525416068 39.27424171495908 37.90698800626321 12.678170753361636 6 21.26587999806292 40.36061921903501 17.38849698945907 20.985003793442996 7 21.45958772538701 35.443671407125215 20.663771812297213 22.43296905519056 8 19.1189526868876 37.193497877286156 22.99149300230835 20.696056433517892 9 19.51121083471888 18.859061486061115 18.823548402718366 42.80617927650164 10-11 19.67989798059694 25.64044617346527 30.546901483478344 24.132754362459444 12-13 22.558879077951218 20.079420168202876 27.732489628565432 29.629211125280474 14-15 22.949522994721463 23.60167234337923 20.813895300973382 32.634909360925924 16-17 24.056078387060325 27.61626499217098 21.330449240504283 26.99720738026441 18-19 22.21181939982889 26.658218857447253 22.06250302668324 29.067458716040612 20-21 21.04311611164022 24.459636152318843 21.774362782288655 32.72288495375228 22-23 23.000371273144037 29.652617475665465 22.261860562720948 25.085150688469547 24-25 23.836542962759687 31.203893525319216 22.121422460410983 22.838141051510114 26-27 24.806695830441168 31.98437424332919 22.20616959111527 21.002760335114367 28-29 23.481412129332195 28.568661318181082 25.87208833072366 22.077838221763063 30-31 29.7535069169801 23.2400845857076 27.66307769294097 19.343330804371337 32-33 27.19656491630212 22.5201375324864 31.499297809488453 18.783999741723033 34-35 29.299100873299004 25.066587031267655 27.34426705838674 18.290045037046603 36-37 24.029443574553262 27.215935689034527 26.705838673747763 22.048782062664447 38-39 21.212610373048797 24.19409514277874 28.118290852152576 26.475003632019888 40-41 19.659720092334016 24.501606159905727 30.584835913412643 25.25383783434761 42-43 24.632358875849487 23.81232949684418 31.58888763337584 19.96642399393049 44-45 19.567708921855072 25.708243878028703 29.11992122552422 25.604125974592 46-47 24.53711924324848 21.798576248204167 27.46533438796429 26.19897012058306 48-49 22.060888795622205 21.701722384542123 28.863258486819802 27.374130333015867 50-51 17.981726904389095 21.990669744467223 32.4113383589727 27.61626499217098 52-53 19.164958272127073 22.042325138420313 36.191060388383995 22.60165620106862 54-55 17.64919530581607 22.181149009669245 33.38875526642884 26.780900418085846 56-57 23.300618250496377 21.867988183828633 29.464559557054997 25.366834008619993 58-59 18.41595505980726 27.747824823645256 32.36049008055013 21.475730035997355 60-61 20.397423687226592 34.93438150736897 24.596038676976224 20.072156128428222 62-63 16.197194466415922 40.44375211867827 22.538701189688293 20.820352225217515 64-65 16.027700205007346 41.217775912444104 21.883323378908457 20.871200503640093 66-67 16.003486739091834 40.523656556199455 21.705757962194706 21.767098742514005 68-69 16.114868682303186 38.54138081324961 22.357907310852475 22.985843193594732 70-71 16.824323233627663 36.61156757978337 22.61779851167896 23.946310674910006 72-73 17.42965988151544 33.89723805065457 22.56695023325639 26.1061518345736 74-75 17.846938610792748 33.182133690616475 22.99230011783887 25.978627580751905 76 17.988990944163746 33.29835832701093 22.788099888618056 25.924550840207267 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 1.5 16 2.0 17 1.0 18 3.0 19 6.5 20 9.5 21 11.0 22 23.5 23 34.5 24 52.0 25 71.0 26 97.5 27 142.0 28 231.5 29 303.0 30 334.0 31 430.5 32 637.0 33 778.0 34 892.5 35 1130.0 36 1403.0 37 1553.0 38 1727.5 39 2240.0 40 2966.5 41 4179.0 42 5003.0 43 5743.5 44 6617.5 45 6189.0 46 5627.0 47 5342.0 48 4583.5 49 3797.5 50 3485.0 51 3140.0 52 2425.5 53 1947.5 54 1839.0 55 1725.5 56 1335.0 57 931.0 58 804.0 59 646.5 60 390.5 61 241.0 62 190.0 63 155.0 64 87.0 65 51.0 66 36.5 67 25.0 68 14.0 69 2.5 70 1.5 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 61949.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.70365946181536 #Duplication Level Percentage of deduplicated Percentage of total 1 62.63208825237081 26.119872798592393 2 16.733113992645634 13.956641753700625 3 7.756918908457519 9.704757138936868 4 4.087478227211148 6.818512001807939 5 2.5778982001161217 5.375389433243474 6 1.641184439713567 4.10660381927069 7 1.1844397135668667 3.4576829327349916 8 0.7431778594929359 2.4794589097483413 9 0.5070640603832011 1.903178420959176 >10 2.0592219856783434 12.187444510807277 >50 0.027095026127346624 0.8523140002259924 >100 0.03870718018192375 2.7829343492227476 >500 0.0038707180181923742 1.5383622011654747 >1k 0.0077414360363847485 8.716847729584012 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT 2816 4.5456746678719595 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT 2584 4.171173061712054 No Hit CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 953 1.5383622011654747 No Hit CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 405 0.653763579718801 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 257 0.4148573826857576 No Hit TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT 210 0.3389885228171561 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT 149 0.2405204280940774 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 136 0.21953542430063439 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT 122 0.19693618944615732 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 119 0.1920934962630551 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT 110 0.1775654167137484 No Hit CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 110 0.1775654167137484 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT 106 0.1711084924696121 No Hit CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 96 0.15496618185927133 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT 88 0.14205233337099873 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTCTT 86 0.13882387124893059 No Hit CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 77 0.12429579169962388 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 65 0.10492501896721496 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006456924244136306 0.0 2 0.0 0.0 0.0 0.006456924244136306 0.0 3 0.0 0.0 0.0 0.006456924244136306 0.0 4 0.0 0.0 0.0 0.006456924244136306 0.0 5 0.0 0.0 0.0 0.008071155305170382 0.0 6 0.0 0.0 0.0 0.008071155305170382 0.0 7 0.0 0.0 0.0 0.008071155305170382 0.0 8 0.0 0.0 0.0 0.008071155305170382 0.0 9 0.0 0.0 0.0 0.009685386366204458 0.0 10 0.0 0.0 0.0 0.014528079549306688 0.0 11 0.0 0.0 0.0 0.016142310610340764 0.0 12 0.0 0.0 0.0 0.016142310610340764 0.0 13 0.0 0.0 0.0 0.017756541671374842 0.0 14 0.0 0.0 0.0 0.020985003793442994 0.0 15 0.0 0.0 0.0 0.020985003793442994 0.0 16 0.0 0.0 0.0 0.024213465915511146 0.0 17 0.0 0.0 0.0 0.025827696976545224 0.0 18 0.0 0.0 0.0 0.029056159098613376 0.0 19 0.0 0.0 0.0 0.03067039015964745 0.0 20 0.0 0.0 0.0 0.03228462122068153 0.0 21 0.0 0.0 0.0 0.040355776525851914 0.0 22 0.0 0.0 0.0 0.045198469708954137 0.0 23 0.0 0.0 0.0 0.04681270076998822 0.0 24 0.0 0.0 0.0 0.05326962501412452 0.0 25 0.0 0.0 0.0 0.05649808713619268 0.0 26 0.0 0.0 0.0 0.05811231819722675 0.0 27 0.0 0.0 0.0 0.07586885986860159 0.0 28 0.0 0.0 0.0 0.09039693941790827 0.0 29 0.0 0.0 0.0 0.1162246363944535 0.0 30 0.0 0.0 0.0 0.1355954091268624 0.0 31 0.0 0.0 0.0 0.1646515682254758 0.0 32 0.0 0.0 0.0 0.21630696217856624 0.0 33 0.0 0.0 0.0 0.24859158339924778 0.0 34 0.0 0.0 0.0 0.30347543947440636 0.0 35 0.0 0.0 0.0 0.3551308334274968 0.0 36 0.0 0.0 0.0 0.4923404736153933 0.0 37 0.0 0.0 0.0 0.677977045634312 0.0 38 0.0 0.0 0.0 0.9992090267800933 0.0 39 0.0 0.0 0.0 1.3914671746113738 0.0 40 0.0 0.0 0.0 1.7756541671374841 0.0 41 0.0 0.0 0.0 2.2922081066683884 0.0 42 0.0 0.0 0.0 2.8652601333354855 0.0 43 0.0 0.0 0.0 3.3398440652795043 0.0 44 0.0 0.0 0.0 3.922581478312806 0.0 45 0.0 0.0 0.0 4.473034270125425 0.0 46 0.0 0.0 0.0 4.962146281618751 0.0 47 0.0 0.0 0.0 5.591696395422041 0.0 48 0.0 0.0 0.0 6.190576119065683 0.0 49 0.0 0.0 0.0 6.690987747986247 0.0 50 0.0 0.0 0.0 7.225298229188526 0.0 51 0.0 0.0 0.0 7.824177952832168 0.0 52 0.0 0.0 0.0 8.39077305525513 0.0 53 0.0 0.0 0.0 9.005795089509112 0.0 54 0.0 0.0 0.0 9.546562494955529 0.0 55 0.0 0.0 0.0 10.105086442073318 0.0 56 0.0 0.0 0.0 10.594198453566644 0.0 57 0.0 0.0 0.0 11.023583915801709 0.0 58 0.0 0.0 0.0 11.47556861289125 0.0 59 0.0 0.0 0.0 11.913025230431485 0.0 60 0.0 0.0 0.0 12.37469531388723 0.0 61 0.0 0.0 0.0 12.792781158695055 0.0 62 0.0 0.0 0.0 13.223780851991155 0.0 63 0.0 0.0 0.0 13.754862871071365 0.0 64 0.0 0.0 0.0 14.20846179922194 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTTCT 15 0.0022149456 70.00001 35 CTATGCG 15 0.0022149456 70.00001 41 GATGTAT 15 0.0022149456 70.00001 24 ATCTAGT 15 0.0022149456 70.00001 36 CGTGCGA 15 0.0022149456 70.00001 10 TTTGTTT 15 0.0022149456 70.00001 33 GCTATGC 15 0.0022149456 70.00001 40 GGATCTA 15 0.0022149456 70.00001 34 TATGCGA 15 0.0022149456 70.00001 42 TTGAGTG 15 0.0022149456 70.00001 43 CCCGTGC 15 0.0022149456 70.00001 8 GAGGTGC 15 0.0022149456 70.00001 35 CTTTGTT 15 0.0022149456 70.00001 32 TTGCGGC 15 0.0022149456 70.00001 24 TTACGTT 15 0.0022149456 70.00001 70 CGAGAAT 15 0.0022149456 70.00001 14 GCCGTAT 15 0.0022149456 70.00001 46 TTTACAC 135 0.0 62.22222 2 TAATGAT 25 2.373346E-4 55.999996 20 GCGAGAA 20 0.00692116 52.5 13 >>END_MODULE