FastQCFastQC Report
Wed 25 May 2016
SRR1780505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460681
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAGG401.8322808E-532.9613232
CGCGGTA651.7564162E-830.4357543
CGGACGA300.00571030329.36590421
TAACGGC752.1518645E-929.34355436
CCGTCAA300.005740738529.33398629
CAGGTAT300.00574684129.3276081
GTCATAC554.959811E-627.9945371
TTAACGG804.285539E-927.5095835
GGCTTAT407.0334866E-427.4946351
CTTAGAC407.0334866E-427.4946351
CTTATAA508.341473E-526.394851
TTTAACG858.176357E-925.8913734
AAACTCG901.4879333E-824.4742524
AACGGCC901.5017577E-824.45296137
TTGTACG450.001406476824.4157949
CCAACTT551.5830906E-424.01880617
TCGTACT551.5912869E-424.00053226
GCTTAAC551.5936352E-423.9953171
ACGACCG551.6042394E-423.9718725
TCGGTGG1900.023.13828842
Produced by FastQC (version 0.11.5)