FastQCFastQC Report
Wed 25 May 2016
SRR1780505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460681
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT261415.674425470119236No Hit
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT208384.523303544101016Illumina PCR Primer Index 9 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC63981.3888135173797052No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC31370.6809484220100243RNA PCR Primer, Index 47 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG31330.680080142224229No Hit
TATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT19760.4289302141829161No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC16160.3507850334613323No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC10740.2331331224860587No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG9110.19775072121489706No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG5870.12742005856547156No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC5800.12590056894032964No Hit
CTTAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC5690.12351279952939236No Hit
TTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC4710.10223994477740564RNA PCR Primer, Index 47 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC4670.10137166499161025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT20900.042.62895244
GCCGTCT24050.037.0455343
CTATACA4400.036.529471
TTTACAC7950.036.2572062
CTTATAA751.8189894E-1235.228421
TTCGGGC250.002350745335.20165324
CGTGCGA250.002351997735.1978310
CCGTGCG250.002351997735.197839
CTTTACA8550.033.7348181
CTTTATA1850.033.302472
TTACACA9200.031.5701263
GCGAAGT353.2159575E-431.42663232
TTATACA38800.029.7159582
CTTATAC39200.029.4880731
GTATCGA300.0057370129.337896
CAAGTTT300.00574005529.33470723
TGAGCGT454.062843E-529.33470727
GACAAGG300.005743101229.33152433
GTAGGTA300.005743101229.33152410
TACATGA300.005743101229.33152428
Produced by FastQC (version 0.11.5)