FastQCFastQC Report
Wed 25 May 2016
SRR1780504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69865
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAT150.00221704169.9928410
AGGTCGA150.00221704169.992849
GACAGCT252.3766697E-455.9942747
TGCGTAT252.3766697E-455.99427455
TCTGCGC200.006927671452.4946322
GAGTGCA200.006927671452.4946317
CGTATTG200.006927671452.4946357
GTCGATG200.006927671452.4946311
GTATTGG200.006927671452.4946358
TTCAGTT200.006927671452.4946338
GTCTGCG200.006927671452.4946321
GCAGTCT200.006927671452.4946318
AATATGG200.006927671452.494637
CTGGTAG200.006927671452.4946330
CAGGGTG200.006927671452.4946354
AGTGCAA200.006927671452.4946318
GTTCGCA200.006927671452.4946311
GATGGAC200.006927671452.4946329
TATTCCG200.006927671452.4946319
AGTCTGC200.006927671452.4946320
Produced by FastQC (version 0.11.5)