FastQCFastQC Report
Wed 25 May 2016
SRR1780504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69865
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT34144.8865669505474845No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT31604.523008659557719No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC11461.640306305016818No Hit
CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC5480.7843698561511486No Hit
TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT3080.44085021112144857No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG2940.42081156516138263No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT1640.23473842410362844No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1520.21756244185214343No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG1390.198955127746368No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1350.19322980032920634No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG1160.16603449509768842No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT1120.16030916768052672No Hit
CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC1120.16030916768052672No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1030.14742718099191296No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT1020.14599584913762256No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT900.12881986688613756No Hit
TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC810.1159378801975238No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTCG150.002216142670.033
TTTACAC1950.062.8205152
ACGACAA401.0477379E-861.2550
CGACAAA352.9353941E-760.051
TACGACA352.9353941E-760.049
TAACAAG352.9353941E-760.031
CTATACA800.056.8751
CTTTACA2200.054.0909081
AAATCGC407.382696E-752.566
CAAACAG407.382696E-752.554
GGACTTA200.0069248752.563
GTCTCTC200.0069248752.532
TTGTACT407.382696E-752.54
CTTGGAA200.0069248752.541
CGCTCAT407.382696E-752.570
CTAAAAT407.382696E-752.563
CTACGAC407.382696E-752.548
TCGCTCA407.382696E-752.569
CGCAGTG200.0069248752.516
CAAGCTC407.382696E-752.534