##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780504_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69865 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.96072425391827 34.0 34.0 34.0 31.0 34.0 2 33.308036928361844 34.0 34.0 34.0 33.0 34.0 3 33.471695412581404 34.0 34.0 34.0 34.0 34.0 4 33.48801259572032 34.0 34.0 34.0 34.0 34.0 5 33.499148357546694 34.0 34.0 34.0 34.0 34.0 6 37.23491018392615 38.0 37.0 38.0 36.0 38.0 7 36.82619337293352 38.0 37.0 38.0 35.0 38.0 8 36.87096543333572 38.0 37.0 38.0 35.0 38.0 9 36.65580762899879 38.0 37.0 38.0 34.0 38.0 10-11 36.371108566521144 38.0 37.0 38.0 34.0 38.0 12-13 36.20384312602877 38.0 37.0 38.0 34.0 38.0 14-15 36.392972160595434 38.0 37.0 38.0 34.0 38.0 16-17 36.507163815930724 38.0 37.0 38.0 34.0 38.0 18-19 36.54326200529593 38.0 37.0 38.0 34.0 38.0 20-21 36.29046732985043 38.0 37.0 38.0 33.5 38.0 22-23 36.350733557575325 38.0 37.0 38.0 33.5 38.0 24-25 35.53959063908967 38.0 36.0 38.0 30.0 38.0 26-27 35.72014599584914 38.0 36.0 38.0 31.0 38.0 28-29 35.668603735776145 38.0 36.5 38.0 31.0 38.0 30-31 35.33553996994203 38.0 36.0 38.0 29.0 38.0 32-33 35.803606956272816 38.0 36.5 38.0 31.5 38.0 34-35 35.56504687611823 38.0 36.0 38.0 31.0 38.0 36-37 35.63436627782151 38.0 36.0 38.0 31.0 38.0 38-39 35.08034781364059 37.5 35.5 38.0 29.0 38.0 40-41 35.010362842625064 37.0 35.5 38.0 29.0 38.0 42-43 35.62836184069276 37.5 36.0 38.0 32.0 38.0 44-45 35.83011522221427 38.0 36.5 38.0 32.0 38.0 46-47 36.09088957274744 38.0 37.0 38.0 34.0 38.0 48-49 35.80539612109068 38.0 36.5 38.0 31.5 38.0 50-51 35.93438774779933 38.0 37.0 38.0 32.0 38.0 52-53 35.784777785729624 38.0 37.0 38.0 31.5 38.0 54-55 36.02978601588778 38.0 37.0 38.0 33.5 38.0 56-57 36.04073570457311 38.0 37.0 38.0 33.0 38.0 58-59 36.05122736706505 38.0 37.0 38.0 34.0 38.0 60-61 35.7488084162313 38.0 36.5 38.0 32.5 38.0 62-63 36.17003506763043 38.0 37.0 38.0 33.5 38.0 64-65 36.137379231374794 38.0 37.0 38.0 33.0 38.0 66-67 35.19385242968582 38.0 36.0 38.0 29.0 38.0 68-69 33.85162813998426 37.0 34.0 38.0 22.5 38.0 70-71 33.04394188792672 37.0 32.5 38.0 16.5 38.0 72-73 32.18313891075646 37.0 29.5 38.0 11.0 38.0 74-75 32.543920417948904 37.0 32.0 38.0 11.0 38.0 76 32.13700708509268 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5360476296044112 1101 2 0.5533376214244115 1101 3 -0.08048017348858139 1101 4 0.3392303168943158 1101 5 0.2381546816840796 1101 6 -0.6066844640687776 1101 7 0.13321855429322937 1101 8 0.675577163199705 1101 9 0.5316391936579734 1101 10-11 0.30386882679310645 1101 12-13 0.7969789075024138 1101 14-15 0.6560816210988634 1101 16-17 -0.3510510242509923 1101 18-19 -0.4983161388636148 1101 20-21 -0.11138851910502723 1101 22-23 0.40112510777640864 1101 24-25 -1.641883172633591 1101 26-27 -0.5754422448700396 1101 28-29 1.084980634581008 1101 30-31 1.3470896221007251 1101 32-33 0.3871791809337566 1101 34-35 0.7341130245373435 1101 36-37 0.9347349881140659 1101 38-39 0.18305479452116913 1101 40-41 1.3843572771649235 1101 42-43 0.8257575002684092 1101 44-45 0.4727528386034052 1101 46-47 0.6959425350217145 1101 48-49 -1.1724470497330586 1101 50-51 -0.7490150807821578 1101 52-53 -1.1017044801522289 1101 54-55 -0.5076072777686917 1101 56-57 -0.8956711063560121 1101 58-59 -1.1718797916831605 1101 60-61 -1.4471185082835518 1101 62-63 -0.30394699171205275 1101 64-65 -1.550686584232551 1101 66-67 -1.97127252123083 1101 68-69 -1.5444643635058597 1101 70-71 -1.065979054321545 1101 72-73 -2.5538388123891735 1101 74-75 -2.414700103910654 1101 76 -1.9629732772145303 1102 1 -0.7477130541562715 1102 2 0.2845999488405866 1102 3 0.18092679561069502 1102 4 0.2260481998660424 1102 5 0.10998242658874346 1102 6 0.30656339917908326 1102 7 0.17102263055884492 1102 8 0.5501662493272548 1102 9 0.5711526716329871 1102 10-11 0.6714066308693774 1102 12-13 0.7599637858719106 1102 14-15 0.10307373155250588 1102 16-17 0.11883720717570156 1102 18-19 -0.44348379172357966 1102 20-21 0.23144514295940866 1102 22-23 0.3672822412412273 1102 24-25 -4.93959849150275 1102 26-27 -0.9272338293539377 1102 28-29 0.5810194248505667 1102 30-31 1.7126879126990175 1102 32-33 0.2240956832348786 1102 34-35 0.8459309075090715 1102 36-37 -2.854268956659112 1102 38-39 -0.23405565912774762 1102 40-41 1.2207478097093016 1102 42-43 0.988791688302598 1102 44-45 0.7978514579327936 1102 46-47 1.0140884916292094 1102 48-49 -3.970622592139364 1102 50-51 -0.80141481779728 1102 52-53 0.0178221470667026 1102 54-55 0.6005452534607656 1102 56-57 0.47252415991618335 1102 58-59 0.2205593930637164 1102 60-61 -6.373351249900914 1102 62-63 -2.585357248122307 1102 64-65 0.1608025676412197 1102 66-67 1.220969424857266 1102 68-69 -2.0117063095939542 1102 70-71 -2.200643748601621 1102 72-73 -4.958473920870432 1102 74-75 -1.8870866916818585 1102 76 -0.8102776822112432 1103 1 0.3220339205412017 1103 2 0.3349299818438851 1103 3 0.014370139945128813 1103 4 0.38814940998705794 1103 5 0.2816545938054631 1103 6 0.3643741802571938 1103 7 0.7045759858943796 1103 8 0.627448977600082 1103 9 0.41449700606643347 1103 10-11 0.15768275848213875 1103 12-13 -0.715338744381107 1103 14-15 -0.06730580640128636 1103 16-17 -0.1840230788529027 1103 18-19 0.5443049871543124 1103 20-21 -0.4968826898238774 1103 22-23 0.35520853387049556 1103 24-25 -3.6085478864422456 1103 26-27 -0.3153801439853936 1103 28-29 -0.509767153807303 1103 30-31 0.6734414880565467 1103 32-33 1.013094583124868 1103 34-35 -0.08330451603328015 1103 36-37 -1.0878498147449207 1103 38-39 -0.3428840419660304 1103 40-41 -0.4762818932609889 1103 42-43 -0.4067253634025718 1103 44-45 1.0571748902760305 1103 46-47 0.9201941021902655 1103 48-49 -2.927470776957847 1103 50-51 -1.0178064569612033 1103 52-53 -0.35396003115111085 1103 54-55 -0.34719952101669094 1103 56-57 0.16287069456964076 1103 58-59 -0.23912157503309572 1103 60-61 -5.195463461122035 1103 62-63 -1.533954607858277 1103 64-65 -0.04225023763931546 1103 66-67 -1.3533980118864122 1103 68-69 -0.7019428332463207 1103 70-71 -0.08438962318908239 1103 72-73 -4.630586132091558 1103 74-75 -2.4225652395366417 1103 76 -2.8468563400770286 1104 1 0.47938721684644037 1104 2 0.24760807892188552 1104 3 0.29908397718251223 1104 4 -0.16649922560821295 1104 5 -0.20289020213754583 1104 6 0.09612979484304418 1104 7 0.44717439207645526 1104 8 0.7218464661294206 1104 9 1.096626926375535 1104 10-11 -0.3871977051739819 1104 12-13 0.49438113004535467 1104 14-15 0.5906753575687702 1104 16-17 0.2597586163897887 1104 18-19 0.7215568586829306 1104 20-21 0.31857251423311794 1104 22-23 0.2420112385312052 1104 24-25 0.8458351128332922 1104 26-27 0.9808285012693716 1104 28-29 1.527699641652731 1104 30-31 0.8956824926448164 1104 32-33 0.4990279325573681 1104 34-35 0.5454566527665179 1104 36-37 1.3044708807392666 1104 38-39 1.26248905631941 1104 40-41 2.029284395075152 1104 42-43 0.112436674752459 1104 44-45 0.6878921083392981 1104 46-47 1.0620559713040052 1104 48-49 0.33567822086875765 1104 50-51 0.38666106296152236 1104 52-53 0.5300172690179252 1104 54-55 0.24309267893501385 1104 56-57 0.22069489162349498 1104 58-59 -0.47320754460566405 1104 60-61 0.3209576931885181 1104 62-63 0.37785413399151224 1104 64-65 0.7874285839013808 1104 66-67 -0.04190320386902613 1104 68-69 2.2651907093762347 1104 70-71 2.0698847066829273 1104 72-73 1.7638838027068005 1104 74-75 0.38527103189537826 1104 76 0.3516464370299488 1105 1 0.7215679915953643 1105 2 0.3189136743307799 1105 3 0.13648235116625074 1105 4 -0.1206838262777481 1105 5 0.04854451809201521 1105 6 0.348066667152942 1105 7 0.6742906044150558 1105 8 -0.3632324434831986 1105 9 0.7325905801297239 1105 10-11 0.6374197027648023 1105 12-13 -0.13202967818037337 1105 14-15 0.6251325550819189 1105 16-17 0.4229221744959659 1105 18-19 0.7104377769038734 1105 20-21 0.5420660579920806 1105 22-23 0.644569822940575 1105 24-25 0.8331139267978998 1105 26-27 0.26868120332580503 1105 28-29 1.2024246536087375 1105 30-31 1.5069689584506492 1105 32-33 1.63520679434599 1105 34-35 -0.184134451968049 1105 36-37 0.505044768831084 1105 38-39 0.11741492910755369 1105 40-41 0.6932968293171484 1105 42-43 0.3389550869954121 1105 44-45 1.161086752050437 1105 46-47 1.1851669230017592 1105 48-49 0.2259460353116154 1105 50-51 1.1134544632484662 1105 52-53 0.8330182254980798 1105 54-55 1.1659047305849413 1105 56-57 0.739190826582842 1105 58-59 0.9806901120179603 1105 60-61 1.451158554020651 1105 62-63 -0.15594548341642422 1105 64-65 1.0225672735235705 1105 66-67 1.3757079869487683 1105 68-69 1.2972806185106194 1105 70-71 1.6905327219866138 1105 72-73 1.3224084320707448 1105 74-75 0.36046232792598687 1105 76 0.32044127203712236 1106 1 -1.0450560493253533 1106 2 0.19583183289855555 1106 3 0.2632939453691492 1106 4 0.17194191967280403 1106 5 0.06420947972883795 1106 6 0.21187740884091966 1106 7 -0.9334459684749703 1106 8 0.45294402710503334 1106 9 -1.1453932220868168 1106 10-11 0.13976411879207973 1106 12-13 1.233272964615871 1106 14-15 -0.5445711959837212 1106 16-17 -0.1587594111817907 1106 18-19 -0.034606980129368026 1106 20-21 -0.31487128216136995 1106 22-23 0.4081034972798818 1106 24-25 -0.9258907620341503 1106 26-27 0.7588772817571794 1106 28-29 0.8674928548022578 1106 30-31 0.8073739030371812 1106 32-33 0.2620183885488814 1106 34-35 0.8439985403593155 1106 36-37 1.1428324926162503 1106 38-39 -0.22119334028716509 1106 40-41 0.8941173749266937 1106 42-43 0.6852892728436615 1106 44-45 0.9197717477878626 1106 46-47 0.7870957380060304 1106 48-49 1.1830005962664316 1106 50-51 -0.015062160792453483 1106 52-53 0.07651779924061941 1106 54-55 -0.005131075041653332 1106 56-57 0.17306763817705217 1106 58-59 0.4914531128704738 1106 60-61 0.8931342573454657 1106 62-63 0.33819347651537157 1106 64-65 -0.8466249685906675 1106 66-67 -0.025347000263508335 1106 68-69 1.7949362024833349 1106 70-71 0.5500808890795383 1106 72-73 0.23518549941942268 1106 74-75 -0.8825577061746088 1106 76 -0.19010860008563668 1107 1 0.9316520252088054 1107 2 -0.24454619237452135 1107 3 0.1791752904109174 1107 4 0.18666670013971753 1107 5 0.17930371832382974 1107 6 0.3709585825397852 1107 7 0.7903439222939284 1107 8 0.10257456897695505 1107 9 -0.14734760203969444 1107 10-11 0.8691761984665334 1107 12-13 0.27479684553255623 1107 14-15 0.5737223358217989 1107 16-17 0.7453287233223875 1107 18-19 0.8100465859447326 1107 20-21 0.8785989470097562 1107 22-23 0.47966593034905713 1107 24-25 0.7586910542005896 1107 26-27 -0.25836409755316936 1107 28-29 0.8345361188845004 1107 30-31 0.9764946997816679 1107 32-33 1.1446485020635606 1107 34-35 0.5150469447285531 1107 36-37 1.3258213553277542 1107 38-39 0.670418889312316 1107 40-41 0.6421763811378725 1107 42-43 1.2351928924624218 1107 44-45 0.8581552346158787 1107 46-47 1.0755006426965394 1107 48-49 0.8441967838869004 1107 50-51 1.1700684881687806 1107 52-53 0.7399896347514314 1107 54-55 0.5608627137782207 1107 56-57 1.1568837658275086 1107 58-59 0.8331483366871382 1107 60-61 1.4053022804767608 1107 62-63 1.0710056418612766 1107 64-65 1.013717181762729 1107 66-67 0.5018670712721516 1107 68-69 1.6178640242867246 1107 70-71 0.1719752990174257 1107 72-73 1.3470269001476254 1107 74-75 0.015212158892857985 1107 76 1.3037069921182578 1108 1 -0.25719814900721616 1108 2 0.41346173745846926 1108 3 0.12835226986543802 1108 4 0.26276906707471426 1108 5 0.031256139325869015 1108 6 -0.49899215637647387 1108 7 -0.196728046947932 1108 8 -0.6049828021632564 1108 9 -0.4497145370390996 1108 10-11 -0.1945147783447112 1108 12-13 0.8821860080941306 1108 14-15 0.5711631624468154 1108 16-17 -0.16707065190288972 1108 18-19 0.11911783429268041 1108 20-21 0.8990603191111646 1108 22-23 0.16640148243364195 1108 24-25 0.8726643811259791 1108 26-27 -0.21185442555989198 1108 28-29 -0.7991296266399459 1108 30-31 0.43226785849847005 1108 32-33 0.7014669569476126 1108 34-35 0.48692006740067484 1108 36-37 1.1776416124465783 1108 38-39 1.452732958758432 1108 40-41 0.8756258584897907 1108 42-43 -0.019270642320705633 1108 44-45 0.8561847912661307 1108 46-47 0.8693730444747416 1108 48-49 0.9332391964785174 1108 50-51 0.7134903312542065 1108 52-53 -0.1529095602503716 1108 54-55 -0.41300488204059604 1108 56-57 0.1816705013795925 1108 58-59 -0.085402666556881 1108 60-61 1.045347937090952 1108 62-63 0.8559029144793229 1108 64-65 0.4801115107306515 1108 66-67 0.7605358205212269 1108 68-69 1.306654124010386 1108 70-71 -0.27889578112194613 1108 72-73 0.017542339292170084 1108 74-75 -0.965948480299744 1108 76 0.41018302239580606 1109 1 -1.0141607826729633 1109 2 0.40077400466981317 1109 3 0.3368107583912092 1109 4 0.19159280851410188 1109 5 0.18915593507259842 1109 6 -0.0920956046523358 1109 7 -0.4411831713021286 1109 8 0.4178455049375458 1109 9 0.6654602797337006 1109 10-11 0.5317377748212095 1109 12-13 0.7378510327246843 1109 14-15 0.6082050944424964 1109 16-17 0.8881858661795334 1109 18-19 0.6671894430875938 1109 20-21 1.0313083066156707 1109 22-23 0.3466610042406799 1109 24-25 0.19465164533358603 1109 26-27 0.6234093014616136 1109 28-29 -1.1162027973716562 1109 30-31 0.8804355864811768 1109 32-33 0.9032691917187208 1109 34-35 0.14805187083693028 1109 36-37 1.3381366262637115 1109 38-39 1.2738671651743871 1109 40-41 0.9820778589703849 1109 42-43 1.5159810698023222 1109 44-45 1.1734261705764766 1109 46-47 0.9794415293960412 1109 48-49 1.011684468615968 1109 50-51 0.0961768625530155 1109 52-53 -0.048187705150049 1109 54-55 -0.567216103955765 1109 56-57 0.22338622888169368 1109 58-59 -0.08310782094832803 1109 60-61 0.9841200144669031 1109 62-63 0.410907119693789 1109 64-65 1.0038649650139533 1109 66-67 0.39102963284850745 1109 68-69 -0.17277636980194444 1109 70-71 -0.022605981770752237 1109 72-73 0.3815096587683158 1109 74-75 -0.12271887558990358 1109 76 0.35789418423648556 1110 1 -0.8506245217736179 1110 2 -0.6677173417119988 1110 3 -0.28954260427046563 1110 4 -0.2454610098784613 1110 5 0.12474772664815958 1110 6 0.03790623934545323 1110 7 0.6127932782118464 1110 8 0.6447889468911328 1110 9 1.0284907583828442 1110 10-11 -0.5348173738840387 1110 12-13 0.6832555268107185 1110 14-15 0.8127439632815623 1110 16-17 0.880809875030721 1110 18-19 0.062042054978739714 1110 20-21 0.9504885178851339 1110 22-23 -0.476196665472969 1110 24-25 -0.8558343797974644 1110 26-27 1.3070056240031676 1110 28-29 0.5763105716122965 1110 30-31 0.10500817413691976 1110 32-33 0.9083796998718299 1110 34-35 1.5184056549838232 1110 36-37 1.769750651184026 1110 38-39 1.1330120770576357 1110 40-41 -1.2260436341409644 1110 42-43 -0.01977931585663839 1110 44-45 0.9235644704105113 1110 46-47 0.5356571190801915 1110 48-49 1.4884237536716824 1110 50-51 0.0673314685960662 1110 52-53 0.3862713449276711 1110 54-55 0.3263325380715756 1110 56-57 0.46089314387339897 1110 58-59 -0.3597733460925596 1110 60-61 0.19848475722920966 1110 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0.3457291238108908 2116 54-55 -0.45533329951081214 2116 56-57 0.1510125320091973 2116 58-59 -1.4200736818716848 2116 60-61 -0.9493202421489784 2116 62-63 0.4706505038156763 2116 64-65 -1.9657478199556806 2116 66-67 -1.2113303167448066 2116 68-69 -1.4090577307825853 2116 70-71 1.0274570265921739 2116 72-73 0.29288266827685305 2116 74-75 0.2369443160700868 2116 76 -0.881337113735789 2117 1 0.8485204675009825 2117 2 0.5430745251117699 2117 3 0.09128461105326124 2117 4 -0.04391413535052635 2117 5 0.1880426902422272 2117 6 0.226431572437086 2117 7 0.7877834591840767 2117 8 0.3095824451841267 2117 9 -1.289675953602412 2117 10-11 -0.5673410150816594 2117 12-13 -1.4183789636572754 2117 14-15 -0.2013047995209405 2117 16-17 -0.8335168417885157 2117 18-19 -0.8208849216670799 2117 20-21 -0.039647135759992125 2117 22-23 -0.2584710544273179 2117 24-25 -2.25678304894155 2117 26-27 -2.3625916447964954 2117 28-29 -1.6670502549987702 2117 30-31 -0.5383949498245286 2117 32-33 -2.388804505089041 2117 34-35 0.3763923011060655 2117 36-37 -1.6606248888625075 2117 38-39 0.6118482956745197 2117 40-41 0.1413692786358567 2117 42-43 0.06087266758697041 2117 44-45 -0.46245927653048113 2117 46-47 -0.7587928643029898 2117 48-49 -1.3608768294275038 2117 50-51 -0.3565937255185645 2117 52-53 0.6726244069537088 2117 54-55 -0.26480314578594033 2117 56-57 0.3345298096336933 2117 58-59 -1.7575950325483376 2117 60-61 -0.8586619843641898 2117 62-63 -0.8428431238285157 2117 64-65 -1.627191782641269 2117 66-67 -1.0650946052745596 2117 68-69 -0.9704162907615697 2117 70-71 -1.1920279293925837 2117 72-73 0.2259893559657229 2117 74-75 0.06771556820515201 2117 76 -0.19210442420747853 2118 1 -0.11483994436008516 2118 2 0.4527459054480758 2118 3 -0.44142691905071274 2118 4 0.10846108612508232 2118 5 0.18490024381425485 2118 6 -0.2398205587433324 2118 7 0.25747890932281337 2118 8 0.3619676957440845 2118 9 -1.4801951258686543 2118 10-11 -0.5541193388873609 2118 12-13 -1.4781690214916594 2118 14-15 0.4312295500929437 2118 16-17 -2.655784752061649 2118 18-19 -0.658079452473082 2118 20-21 -0.7593175131878667 2118 22-23 0.6200600190190073 2118 24-25 0.7101878332835767 2118 26-27 -2.847631593982463 2118 28-29 -0.8380962884959189 2118 30-31 -0.6292417388460194 2118 32-33 -0.006311943326593905 2118 34-35 -0.10316508197153951 2118 36-37 -2.438046076974693 2118 38-39 -0.5447558563664074 2118 40-41 -0.1363527820066679 2118 42-43 0.5269409322210805 2118 44-45 -2.2580163921658283 2118 46-47 -0.13245983184218346 2118 48-49 0.4606890449415033 2118 50-51 -0.8377724772318658 2118 52-53 -1.7999293322232361 2118 54-55 -0.9070155617923632 2118 56-57 0.6279975326972433 2118 58-59 -0.7843058271861238 2118 60-61 -1.0003729461533766 2118 62-63 -0.9785032047298046 2118 64-65 -0.18678125878876273 2118 66-67 -0.0807399768521293 2118 68-69 -1.4349969078845533 2118 70-71 0.36893218828200247 2118 72-73 0.5053327852439651 2118 74-75 -0.36736282580414326 2118 76 -0.21741114703701214 2119 1 0.670663014219798 2119 2 -1.529502615261979 2119 3 0.04673876142266664 2119 4 -0.6089945351766914 2119 5 -0.3032654366591174 2119 6 -0.6836075409918578 2119 7 -0.7178662583300479 2119 8 -0.11436366520266006 2119 9 -0.409189208708888 2119 10-11 -0.33863610417336076 2119 12-13 0.1347501106582385 2119 14-15 -0.08282370434493203 2119 16-17 -0.10253031419182435 2119 18-19 -0.14156071480049803 2119 20-21 -1.0850078484935821 2119 22-23 -0.3030169040579125 2119 24-25 0.9345468076425476 2119 26-27 0.7364413843212816 2119 28-29 -0.004023310192167173 2119 30-31 0.9464485964597031 2119 32-33 0.19610423024342083 2119 34-35 0.13524714682530714 2119 36-37 -0.12542280281295604 2119 38-39 -1.0270043770764659 2119 40-41 -1.1728419338804414 2119 42-43 -0.12937070485979518 2119 44-45 -0.04894341386207657 2119 46-47 0.28075515829587516 2119 48-49 -0.5994687459855186 2119 50-51 0.694525353142879 2119 52-53 0.8364118906564499 2119 54-55 0.22981875773426452 2119 56-57 -0.5877322503402311 2119 58-59 0.3547474272517448 2119 60-61 0.5067769552272949 2119 62-63 -0.060357248122301144 2119 64-65 -0.19047948364083567 2119 66-67 0.6628497667376081 2119 68-69 1.5041056562180088 2119 70-71 0.3455100975522214 2119 72-73 1.3985773611808483 2119 74-75 0.5263748467796816 2119 76 0.7175000955665354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 13.0 23 33.0 24 45.0 25 105.0 26 244.0 27 382.0 28 656.0 29 1048.0 30 1594.0 31 2504.0 32 3524.0 33 5415.0 34 7822.0 35 10038.0 36 13789.0 37 22651.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.52279395977958 21.39984255349603 10.661990982609318 32.41537250411508 2 18.25377513776569 18.109210620482358 42.1512917769985 21.485722464753454 3 16.3701424175195 25.34029914835755 35.31811350461604 22.971444929506905 4 11.264581693265583 22.544908036928362 34.75846274958849 31.43204752021756 5 10.886710083732913 38.34394904458599 37.135904959564876 13.633435912116223 6 22.97717025692407 38.590138123523936 16.414513705002502 22.01817791454949 7 23.29778859228512 35.1148643813068 18.561511486438132 23.02583553996994 8 20.267659056752308 35.9521935160667 22.202819723752953 21.57732770342804 9 19.915551420596866 19.785300221856435 17.678379732340943 42.62076862520575 10-11 19.903385099835397 24.971731195877762 30.41938023330709 24.705503470979746 12-13 22.953553281328276 19.378086309310813 27.62971444929507 30.03864596006584 14-15 23.161096400200385 23.15465540685608 20.433693551849995 33.25055464109354 16-17 24.400629786015887 27.473699277177417 20.901739068202964 27.223931868603735 18-19 22.70593287053603 26.033779431761257 21.54011307521649 29.720174622486223 20-21 21.127173835253704 24.39991412008874 21.291061332569956 33.181850712087595 22-23 23.177556716524727 29.275746081729046 21.854290417233237 25.69240678451299 24-25 24.146568381879337 31.27388535031847 21.656050955414013 22.92349531238818 26-27 25.21577327703428 28.56866814570958 25.30093752236456 20.914621054891576 28-29 20.39719458956559 22.982179918414083 30.829456809561297 25.791168682459027 30-31 28.511414871537966 21.5007514492235 25.466256351535105 24.52157732770343 32-33 28.716095326701495 22.197810062262935 29.086810276962716 19.999284334072858 34-35 29.13976955557146 25.18929363772991 27.6175481285336 18.05338867816503 36-37 20.12881986688614 26.950547484434267 30.89171974522293 22.028912903456664 38-39 20.708509267873758 23.592642954268946 28.54648250196808 27.152365275889217 40-41 19.797466542617904 24.37772847634724 30.649108995920706 25.17569598511415 42-43 24.593859586345094 23.006512559937022 31.985972947827957 20.41365490588993 44-45 19.486867530236886 24.93666356544765 29.873327130895298 25.703141773420167 46-47 24.450010734988908 20.737851570886708 27.86946253488871 26.94267515923567 48-49 21.93587633292779 20.64982466184785 29.553424461461393 27.86087454376297 50-51 17.410720675588635 21.256709368066986 33.23695698847778 28.0956129678666 52-53 18.938667430043658 21.07063622700923 37.214628211550846 22.776068131396265 54-55 17.47441494310456 21.264581693265583 33.91182995777571 27.34917340585415 56-57 22.236456022328777 21.286767337007085 30.333500322049666 26.143276318614472 58-59 17.70700636942675 27.52665855578616 32.76891147212481 21.997423602662277 60-61 19.77599656480355 34.50511701137909 25.00250483074501 20.716381593072352 62-63 15.71029843269162 40.3435196450297 22.740284835039006 21.205897087239673 64-65 15.457668360409361 41.33113862449009 21.437057181707576 21.77413583339297 66-67 15.461246690045085 40.38288127102268 21.193730766478208 22.96214127245402 68-69 15.66378014742718 38.904315465540684 21.057038574393474 24.37486581263866 70-71 16.45101266728691 36.34867243970514 21.717598225148503 25.482716667859446 72-73 16.916195519931296 33.33929721605954 22.19136906891863 27.553138195090533 74-75 17.561726186216276 32.52558505689544 22.67229657196021 27.240392184928076 76 17.39354469333715 32.52844772060402 22.64653259858298 27.431474987475845 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.0 15 1.0 16 3.0 17 6.0 18 6.0 19 7.5 20 17.0 21 25.0 22 24.0 23 28.5 24 39.0 25 44.0 26 83.0 27 140.0 28 193.0 29 228.0 30 293.0 31 443.0 32 658.5 33 789.0 34 864.0 35 1082.5 36 1388.5 37 1551.0 38 1796.0 39 2387.0 40 3106.0 41 4490.0 42 5501.0 43 6319.5 44 7227.5 45 6954.0 46 6591.0 47 6020.5 48 5008.5 49 4232.0 50 3897.0 51 3636.0 52 3021.0 53 2397.0 54 2127.0 55 2003.0 56 1608.5 57 1208.0 58 1078.0 59 913.0 60 647.0 61 486.0 62 426.0 63 350.0 64 242.0 65 187.0 66 142.5 67 121.0 68 98.0 69 57.5 70 27.5 71 15.0 72 13.5 73 7.0 74 2.5 75 3.0 76 2.0 77 0.5 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 69865.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.62463322121234 #Duplication Level Percentage of deduplicated Percentage of total 1 64.97417968374121 28.99448937236098 2 16.81688424158835 15.008945824089315 3 7.630625140327806 10.215415444070707 4 3.7688039259710684 6.727259715164961 5 1.9758155050197264 4.408502111214485 6 1.3311094717259517 3.5640163171831394 7 0.8692305225005614 2.7152365275889214 8 0.6030086281553709 2.1527231088527876 9 0.4233890367899413 1.7004222428970157 >10 1.5428039901209225 9.67580333500322 >50 0.016037463514770502 0.5167107993988406 >100 0.03528241973249511 2.4862234309024545 >500 0.0032074927029541005 0.7843698561511486 >1k 0.009622478108862303 11.049881915122022 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT 3414 4.8865669505474845 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT 3160 4.523008659557719 No Hit CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 1146 1.640306305016818 No Hit CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 548 0.7843698561511486 No Hit TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT 308 0.44085021112144857 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 294 0.42081156516138263 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT 164 0.23473842410362844 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 152 0.21756244185214343 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 139 0.198955127746368 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 135 0.19322980032920634 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 116 0.16603449509768842 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT 112 0.16030916768052672 No Hit CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 112 0.16030916768052672 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 103 0.14742718099191296 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT 102 0.14599584913762256 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT 90 0.12881986688613756 No Hit TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 81 0.1159378801975238 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0042939955628712514 0.0 2 0.0 0.0 0.0 0.007156659271452086 0.0 3 0.0 0.0 0.0 0.007156659271452086 0.0 4 0.0 0.0 0.0 0.011450654834323337 0.0 5 0.0 0.0 0.0 0.012881986688613754 0.0 6 0.0 0.0 0.0 0.012881986688613754 0.0 7 0.0 0.0 0.0 0.012881986688613754 0.0 8 0.0 0.0 0.0 0.012881986688613754 0.0 9 0.0 0.0 0.0 0.014313318542904171 0.0 10 0.0 0.0 0.0 0.014313318542904171 0.0 11 0.0 0.0 0.0 0.014313318542904171 0.0 12 0.0 0.0 0.0 0.017175982251485006 0.0 13 0.0 0.0 0.0 0.018607314105775423 0.0 14 0.0 0.0 0.0 0.021469977814356257 0.0 15 0.0 0.0 0.0 0.027195305231517926 0.0 16 0.0 0.0 0.0 0.028626637085808343 0.0 17 0.0 0.0 0.0 0.030057968940098764 0.0 18 0.0 0.0 0.0 0.032920632648679594 0.0 19 0.0 0.0 0.0 0.03435196450297001 0.0 20 0.0 0.0 0.0 0.03578329635726043 0.0 21 0.0 0.0 0.0 0.04437128748300293 0.0 22 0.0 0.0 0.0 0.04580261933729335 0.0 23 0.0 0.0 0.0 0.04866528304587418 0.0 24 0.0 0.0 0.0 0.05152794675445502 0.0 25 0.0 0.0 0.0 0.05439061046303585 0.0 26 0.0 0.0 0.0 0.06297860158877835 0.0 27 0.0 0.0 0.0 0.09446790238316753 0.0 28 0.0 0.0 0.0 0.12738853503184713 0.0 29 0.0 0.0 0.0 0.15315250840907466 0.0 30 0.0 0.0 0.0 0.20324912330923925 0.0 31 0.0 0.0 0.0 0.27910971158663134 0.0 32 0.0 0.0 0.0 0.33922564946682887 0.0 33 0.0 0.0 0.0 0.4079295784727689 0.0 34 0.0 0.0 0.0 0.4966721534387748 0.0 35 0.0 0.0 0.0 0.6011593788019752 0.0 36 0.0 0.0 0.0 0.7657625420453732 0.0 37 0.0 0.0 0.0 1.0305589350891005 0.0 38 0.0 0.0 0.0 1.4699778143562585 0.0 39 0.0 0.0 0.0 1.9079653617691261 0.0 40 0.0 0.0 0.0 2.5563586917626853 0.0 41 0.0 0.0 0.0 3.2763186144707652 0.0 42 0.0 0.0 0.0 4.186645673799471 0.0 43 0.0 0.0 0.0 5.106992056108209 0.0 44 0.0 0.0 0.0 6.014456451728333 0.0 45 0.0 0.0 0.0 6.743004365562156 0.0 46 0.0 0.0 0.0 7.504472912044657 0.0 47 0.0 0.0 0.0 8.274529449652903 0.0 48 0.0 0.0 0.0 8.99305804050669 0.0 49 0.0 0.0 0.0 9.665784012023188 0.0 50 0.0 0.0 0.0 10.28984470049381 0.0 51 0.0 0.0 0.0 11.00694195949331 0.0 52 0.0 0.0 0.0 11.784155156373005 0.0 53 0.0 0.0 0.0 12.5914263221928 0.0 54 0.0 0.0 0.0 13.407285479138338 0.0 55 0.0 0.0 0.0 14.148715379660775 0.0 56 0.0 0.0 0.0 14.838617333428756 0.0 57 0.0 0.0 0.0 15.476991340442282 0.0 58 0.0 0.0 0.0 16.048092750304157 0.0 59 0.0 0.0 0.0 16.644958133543263 0.0 60 0.0 0.0 0.0 17.191726901882202 0.0 61 0.0 0.0 0.0 17.6998497101553 0.0 62 0.0 0.0 0.0 18.225148500679882 0.0 63 0.0 0.0 0.0 18.79624991054176 0.0 64 0.0 0.0 0.0 19.49044585987261 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTCTCG 15 0.0022161426 70.0 33 TTTACAC 195 0.0 62.820515 2 ACGACAA 40 1.0477379E-8 61.25 50 CGACAAA 35 2.9353941E-7 60.0 51 TACGACA 35 2.9353941E-7 60.0 49 TAACAAG 35 2.9353941E-7 60.0 31 CTATACA 80 0.0 56.875 1 CTTTACA 220 0.0 54.090908 1 AAATCGC 40 7.382696E-7 52.5 66 CAAACAG 40 7.382696E-7 52.5 54 GGACTTA 20 0.00692487 52.5 63 GTCTCTC 20 0.00692487 52.5 32 TTGTACT 40 7.382696E-7 52.5 4 CTTGGAA 20 0.00692487 52.5 41 CGCTCAT 40 7.382696E-7 52.5 70 CTAAAAT 40 7.382696E-7 52.5 63 CTACGAC 40 7.382696E-7 52.5 48 TCGCTCA 40 7.382696E-7 52.5 69 CGCAGTG 20 0.00692487 52.5 16 CAAGCTC 40 7.382696E-7 52.5 34 >>END_MODULE