FastQCFastQC Report
Wed 25 May 2016
SRR1780503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644226
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA207.834779E-444.02177440
CTTTATA407.088258E-427.460222
GTACAAG651.8178334E-523.6819321
CCGGTCG752.083665E-623.438189
CTTTACA3600.022.6012081
GGCAATT500.002565936222.0194524
CGTTAAT500.002584539621.99207526
CTATACA1500.021.9903661
GACCATA602.8792716E-421.9903661
TTAATAC703.2484386E-521.9681783
GTATACC856.1293067E-620.6968151
CGGTCGT755.4565364E-520.53870410
CTTATAA755.4860062E-520.5243421
TGGCGAC654.8763375E-420.33988616
GATACTA654.940705E-420.30037730
TTTACAC3800.019.6557392
GATGTAT1352.562956E-919.57284424
CGTGCGA1352.582965E-919.5606710
GATCCTA901.0045589E-519.546991
GGAAACG808.962228E-519.25503322
Produced by FastQC (version 0.11.5)