FastQCFastQC Report
Wed 25 May 2016
SRR1780503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644226
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT383705.95598439057101No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT312084.844262727676312Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC99421.5432472455318476No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC52650.8172597815052483No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG46090.7154321620052589No Hit
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT31350.4866304681897347No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC23360.3626056694389857No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC15600.2421510463719254No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG12760.1980671379298569No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC10250.159105655468733No Hit
CTTATAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG9110.14141000208001542No Hit
CTTAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC9110.14141000208001542No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC8030.12464569886965132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT32650.042.38374344
TTGCGAT452.3477696E-839.11194216
GCCGTCT36550.037.8010743
TTTACAC13600.037.206672
CTTTACA13750.036.9808771
AGTCCAG301.3002982E-436.66744241
GACGATT301.3008922E-436.66459723
CTATACA7000.035.8488121
GGTACAA505.941547E-835.19801330
CTTATAA1200.034.853011
TCTTTAT2100.033.5427441
GGTCATA401.8117451E-533.0186421
GCACTAA401.8117451E-533.0186421
CGTGCGA401.81753E-533.0032610
TTACACA15500.032.929733
CTTTATA2200.032.0006752
GTACAAT551.3727185E-731.99819231
GGTACGG300.005727218529.3499011
ATTGCGA602.939214E-729.33623115
TCGGTCT300.005742452629.33395444
Produced by FastQC (version 0.11.5)