FastQCFastQC Report
Wed 25 May 2016
SRR1780502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55350
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACT150.00221517669.9882649
GTGCTTC150.00221517669.9882637
GAAACGT150.00221517669.9882628
ACAGCTT150.00221517669.9882610
CCTGGCG252.3731064E-455.990630
ATAGGTG200.00692189352.4911965
TGGCGGT200.00692189352.49119632
TTCACAG200.00692189352.49119639
AGAACTA200.00692189352.49119650
ATCGTAC200.00692189352.49119610
GGAAGGT200.00692189352.49119614
GCTTCAT200.00692189352.49119639
TACGAGC200.00692189352.4911962
GGCGGTG200.00692189352.49119633
ACATGAG200.00692189352.49119617
TTACGCT200.00692189352.49119667
AAGGACC200.00692189352.49119625
CTGGCGG200.00692189352.49119631
GATAGGT200.00692189352.4911964
ACGCGCA200.00692189352.49119612