##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780502_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55350 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.451237579042456 33.0 23.0 34.0 12.0 34.0 2 27.964462511291778 34.0 23.0 34.0 11.0 34.0 3 28.311743450767842 34.0 23.0 34.0 11.0 34.0 4 28.664028906955735 34.0 23.0 34.0 11.0 34.0 5 28.629647696476965 34.0 23.0 34.0 11.0 34.0 6 30.949756097560975 37.0 26.0 38.0 11.0 38.0 7 30.006106594399277 36.0 24.0 38.0 11.0 38.0 8 29.107895212285456 34.0 21.0 38.0 11.0 38.0 9 29.92917795844625 36.0 23.0 38.0 12.0 38.0 10-11 30.04900632339657 36.0 23.5 38.0 11.0 38.0 12-13 30.366522131887983 37.0 24.5 38.0 11.0 38.0 14-15 30.018455284552843 36.0 23.0 38.0 11.0 38.0 16-17 29.7239837398374 36.0 22.5 38.0 11.0 38.0 18-19 29.8186630532972 36.0 22.5 38.0 11.0 38.0 20-21 29.967009936766033 36.0 23.0 38.0 11.0 38.0 22-23 29.722619692863596 36.0 22.5 38.0 11.0 38.0 24-25 30.042890695573625 36.5 23.0 38.0 11.0 38.0 26-27 29.990406504065042 36.5 23.0 38.0 11.0 38.0 28-29 29.879430894308943 37.0 22.5 38.0 11.0 38.0 30-31 29.77728997289973 37.0 22.0 38.0 11.0 38.0 32-33 29.741680216802166 36.0 22.5 38.0 11.0 38.0 34-35 29.740027100271003 36.0 22.5 38.0 11.0 38.0 36-37 29.631616982836498 36.0 22.0 38.0 11.0 38.0 38-39 29.851400180668474 37.0 22.0 38.0 11.0 38.0 40-41 29.602032520325203 36.0 21.5 38.0 11.0 38.0 42-43 29.855329719963866 36.5 22.5 38.0 11.0 38.0 44-45 29.149024390243902 35.0 21.0 38.0 11.0 38.0 46-47 29.718202348690152 36.0 22.5 38.0 11.0 38.0 48-49 29.640767841011744 36.0 23.0 38.0 11.0 38.0 50-51 29.59227642276423 35.5 22.0 38.0 11.0 38.0 52-53 29.5936495031617 36.0 22.5 38.0 11.0 38.0 54-55 29.440776874435414 35.5 22.0 38.0 11.0 38.0 56-57 29.317398373983742 35.5 21.0 38.0 11.0 38.0 58-59 29.23281842818428 35.0 21.5 38.0 11.0 38.0 60-61 29.296892502258356 35.5 22.0 38.0 11.0 38.0 62-63 29.22485998193315 35.5 21.5 38.0 11.0 38.0 64-65 29.322204155374887 35.5 21.5 38.0 11.0 38.0 66-67 29.405998193315266 35.5 22.5 38.0 11.0 38.0 68-69 28.82710027100271 34.5 21.0 38.0 11.0 38.0 70-71 28.613550135501356 34.0 20.0 38.0 11.0 38.0 72-73 28.629268292682926 34.0 20.0 38.0 11.0 38.0 74-75 28.46577235772358 34.0 20.0 38.0 11.0 38.0 76 27.71427280939476 32.0 19.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.2214644741450087 1101 2 -1.0322382881094718 1101 3 -0.1846441874195257 1101 4 -3.1897890127644253 1101 5 -0.46830937412849494 1101 6 -1.6101681401450882 1101 7 -1.8838891327425067 1101 8 -0.811949991494533 1101 9 -1.8375731915020452 1101 10-11 -1.6907544851906557 1101 12-13 -1.217295651582642 1101 14-15 -1.1382394279572488 1101 16-17 -1.660589750658847 1101 18-19 -0.37148951301599453 1101 20-21 -1.4653451531997739 1101 22-23 -0.5426489628437885 1101 24-25 -1.9955569322694302 1101 26-27 -1.4107952991911858 1101 28-29 -0.3446005934860956 1101 30-31 -1.1861286914049174 1101 32-33 -0.5337887005872943 1101 34-35 -0.8346218761500026 1101 36-37 -0.8115567621711008 1101 38-39 -0.9958957826899208 1101 40-41 -0.937210429720043 1101 42-43 -2.177120195529792 1101 44-45 -1.1388186528463322 1101 46-47 -0.47131110062635173 1101 48-49 -1.9733124580898007 1101 50-51 -1.744214904561236 1101 52-53 -0.708026105167189 1101 54-55 -1.3923542938016027 1101 56-57 -1.955682041452377 1101 58-59 -1.2239067630285447 1101 60-61 -1.6766907012554704 1101 62-63 -1.6464398053147562 1101 64-65 -1.3340211802412263 1101 66-67 -1.8169953643112073 1101 68-69 -1.6284269782917633 1101 70-71 -0.5189186774631089 1101 72-73 -1.336898842906816 1101 74-75 -1.8416769561144193 1101 76 -1.463913793254541 1102 1 0.8192762665957325 1102 2 0.3308857054493046 1102 3 0.5817004180555152 1102 4 1.0044138857863025 1102 5 1.0500481137942117 1102 6 0.42332622378405915 1102 7 0.4589450057438036 1102 8 1.3080178023863063 1102 9 1.0245846184818497 1102 10-11 0.6436255470154606 1102 12-13 -2.2663294680077612 1102 14-15 -0.28735375968027554 1102 16-17 -0.8441646298858991 1102 18-19 0.5854348026039737 1102 20-21 1.1238657968807395 1102 22-23 1.1182206023736043 1102 24-25 1.4227200403553688 1102 26-27 -3.2426793571621992 1102 28-29 0.682613577206336 1102 30-31 1.0092014213164973 1102 32-33 -5.272677589476185 1102 34-35 0.29130404977592406 1102 36-37 1.6250776983764048 1102 38-39 0.7758433477448641 1102 40-41 0.5893596185891354 1102 42-43 1.3375335886892117 1102 44-45 -4.834712372653097 1102 46-47 -0.3156750297728905 1102 48-49 1.0125973647765463 1102 50-51 -3.8021054037560837 1102 52-53 -0.3799262661977849 1102 54-55 0.8799484437185292 1102 56-57 -1.4710604633525364 1102 58-59 1.080119001866784 1102 60-61 0.6741144517235931 1102 62-63 -2.947969595974982 1102 64-65 0.046011025877934486 1102 66-67 -0.006447860285440754 1102 68-69 -5.795174160256337 1102 70-71 0.004672304823525764 1102 72-73 0.25134592359881935 1102 74-75 -4.057055378014578 1102 76 -0.7259105726426256 1103 1 0.516245963565428 1103 2 -0.4757207315919132 1103 3 -0.5518399546152928 1103 4 -0.4209578439332553 1103 5 0.24626494044581904 1103 6 -1.9135832435601898 1103 7 0.41245526360188833 1103 8 0.9338412542967127 1103 9 -0.054937308866165324 1103 10-11 1.1041469097107388 1103 12-13 -1.174149908436899 1103 14-15 0.09665715693933308 1103 16-17 0.2770004369313419 1103 18-19 -0.22310086462734446 1103 20-21 -0.34324318109780094 1103 22-23 -0.28658835678318084 1103 24-25 0.48003229331979824 1103 26-27 -0.6525701909660775 1103 28-29 0.8596322107467067 1103 30-31 -0.09488290015912781 1103 32-33 -3.797569364367959 1103 34-35 -0.8125920541201808 1103 36-37 -0.4590555596698991 1103 38-39 0.23570594891181074 1103 40-41 0.5263537838441721 1103 42-43 1.2155685971590025 1103 44-45 -3.688890802918486 1103 46-47 0.6132728339575806 1103 48-49 0.2551627703854322 1103 50-51 -0.08033804068437078 1103 52-53 0.4365145393734551 1103 54-55 0.2591127576654486 1103 56-57 0.14751661174627628 1103 58-59 -0.2192410583628437 1103 60-61 0.8997402751755068 1103 62-63 -0.10230801118296284 1103 64-65 0.7513564051363595 1103 66-67 -0.6963123436281862 1103 68-69 -2.535810335675123 1103 70-71 0.45804139949696676 1103 72-73 0.9214946154866439 1103 74-75 -2.7793873938248552 1103 76 -0.8620859914267385 1104 1 -1.2742196683636173 1104 2 0.13284752941537192 1104 3 0.5570450628063099 1104 4 0.8614304994306998 1104 5 0.21150092397448716 1104 6 0.5281335762054091 1104 7 0.4657495303107879 1104 8 0.7309587709264243 1104 9 0.5229055799523366 1104 10-11 0.7171497605184669 1104 12-13 -0.02163169494307482 1104 14-15 0.9659159469298295 1104 16-17 -0.0735301300626432 1104 18-19 1.2872729079415492 1104 20-21 1.2918138350567752 1104 22-23 0.6205624192700334 1104 24-25 1.1466065726989534 1104 26-27 1.0064811235730424 1104 28-29 -0.1364479286261151 1104 30-31 -0.4072443192278641 1104 32-33 -0.9200353130534182 1104 34-35 1.016913805873486 1104 36-37 0.8488316750466041 1104 38-39 0.20125868885479647 1104 40-41 1.2748491908761643 1104 42-43 1.5050839598450985 1104 44-45 -2.238344398103788 1104 46-47 1.065996939120712 1104 48-49 1.503539393762054 1104 50-51 1.3103018072725483 1104 52-53 0.829688438644915 1104 54-55 1.2047098435064392 1104 56-57 0.9751219332540373 1104 58-59 0.8557906172785898 1104 60-61 0.3312812951311592 1104 62-63 1.1549377917945485 1104 64-65 0.5407529841670815 1104 66-67 1.4427833168089386 1104 68-69 -1.9756266169548162 1104 70-71 0.42399008495784685 1104 72-73 1.2030514732630095 1104 74-75 0.6330294576163844 1104 76 1.2850473630236863 1105 1 -0.3344871742644848 1105 2 0.05641165215337196 1105 3 0.06290658916350722 1105 4 -0.1575309482482936 1105 5 0.5180705541869166 1105 6 0.6918629231762985 1105 7 0.847771560208507 1105 8 0.6291491721852758 1105 9 -0.5847655451460128 1105 10-11 1.7444904371510432 1105 12-13 0.1174713735069659 1105 14-15 0.9444367966544611 1105 16-17 0.9153959124235911 1105 18-19 0.9857386829218768 1105 20-21 1.1136273677082293 1105 22-23 0.6584263059734177 1105 24-25 0.3473343461057219 1105 26-27 1.136516528765803 1105 28-29 0.2448856669679067 1105 30-31 0.687228536791018 1105 32-33 0.8020535933195134 1105 34-35 1.6913040497759226 1105 36-37 1.4757789606484977 1105 38-39 1.0223837872025534 1105 40-41 0.5710332978785218 1105 42-43 0.42168506133998207 1105 44-45 1.8178121715264268 1105 46-47 1.1492384812135512 1105 48-49 1.0849910066652804 1105 50-51 0.34551030451881104 1105 52-53 0.11359874548991655 1105 54-55 1.0701307719092696 1105 56-57 0.1412116264090315 1105 58-59 0.4838590673179297 1105 60-61 0.8302388088998462 1105 62-63 0.39394251248691603 1105 64-65 0.5506861076918703 1105 66-67 0.9864693907664517 1105 68-69 0.9270091029507697 1105 70-71 0.6512127442812137 1105 72-73 0.9212851475352366 1105 74-75 0.18747477626311948 1105 76 -0.7974860752139215 1106 1 0.03774473506420151 1106 2 0.13993388406270313 1106 3 0.11900946623412878 1106 4 0.49129206831665684 1106 5 0.40316209734297814 1106 6 -0.23834631832326636 1106 7 0.9743190754657007 1106 8 1.8281901486456071 1106 9 2.301415797486939 1106 10-11 1.2019882575520882 1106 12-13 1.4528244685374787 1106 14-15 0.9754742857564622 1106 16-17 1.7722180571489616 1106 18-19 1.6706776541511736 1106 20-21 0.9412864784318558 1106 22-23 1.960954640759816 1106 24-25 1.7936072318947325 1106 26-27 1.1220875836916981 1106 28-29 0.37121522232972026 1106 30-31 1.308065502789276 1106 32-33 1.691286374487781 1106 34-35 1.298060806532682 1106 36-37 1.7207004950861595 1106 38-39 1.2146898028956663 1106 40-41 1.0766784591688463 1106 42-43 0.8528097344001395 1106 44-45 1.6777729387452567 1106 46-47 1.0595697802545274 1106 48-49 1.4697556099782716 1106 50-51 1.5173716820253524 1106 52-53 1.0974043252632413 1106 54-55 1.2101482087009003 1106 56-57 1.8188925331221988 1106 58-59 0.9741162599944779 1106 60-61 2.089850840286072 1106 62-63 1.7523339684590162 1106 64-65 1.1339467877267388 1106 66-67 1.489586217270375 1106 68-69 2.3498512999865113 1106 70-71 1.7104106518662192 1106 72-73 1.324837893829919 1106 74-75 0.11803275359529053 1106 76 0.7454956161548658 1107 1 -0.4538992758105813 1107 2 0.14681058324709184 1107 3 0.5267737393830139 1107 4 0.17352459670303233 1107 5 -0.2392495450686667 1107 6 1.0844187544418062 1107 7 0.5492031311189862 1107 8 0.8308288820535736 1107 9 0.3542304464554391 1107 10-11 0.23609188780956458 1107 12-13 0.43543281653044374 1107 14-15 0.1948090050911837 1107 16-17 0.8111393749164328 1107 18-19 0.667739064674123 1107 20-21 1.2764256116479125 1107 22-23 0.11376129356646913 1107 24-25 0.4207262147829489 1107 26-27 1.0404867520908674 1107 28-29 -0.216022903369943 1107 30-31 0.7359401229715843 1107 32-33 0.865882568314742 1107 34-35 0.39189576575225615 1107 36-37 0.20952585891151898 1107 38-39 0.4991732508406521 1107 40-41 0.7771222461510945 1107 42-43 1.16362054521095 1107 44-45 0.64001106444492 1107 46-47 1.0222676852601253 1107 48-49 0.8667050742354263 1107 50-51 1.0740844598919743 1107 52-53 0.7425546531058771 1107 54-55 0.7272599950434575 1107 56-57 1.3418415176096445 1107 58-59 1.4624618541093035 1107 60-61 0.9317467302588831 1107 62-63 1.2543929801334102 1107 64-65 -0.17798348574199352 1107 66-67 0.9484659549962657 1107 68-69 1.0118535389118293 1107 70-71 1.0356945156116204 1107 72-73 0.6257134735516523 1107 74-75 0.6434205674446467 1107 76 0.8846288322111597 1108 1 -0.14871490338219218 1108 2 -0.5713601914697861 1108 3 -0.7032117616162381 1108 4 0.41021098723557614 1108 5 -0.15366625418736035 1108 6 -0.10253998218580307 1108 7 0.18449448266097335 1108 8 -0.5848222014528339 1108 9 -1.2246715481368255 1108 10-11 -0.29161295955906397 1108 12-13 0.8516521041200811 1108 14-15 -0.2723331243026017 1108 16-17 -0.4207074842865133 1108 18-19 -0.7436994713186706 1108 20-21 -0.11440083139452284 1108 22-23 -0.2927593381197262 1108 24-25 -1.68748727320639 1108 26-27 -1.3176385662993546 1108 28-29 -0.7836979690073136 1108 30-31 -0.8907889808334204 1108 32-33 -0.4685937042664712 1108 34-35 -1.3312125065154667 1108 36-37 -0.7020890162795119 1108 38-39 -0.537838387546433 1108 40-41 -0.21719571301658291 1108 42-43 0.12719670415134843 1108 44-45 0.4460967261087774 1108 46-47 0.37668988048721275 1108 48-49 -0.44927517577437115 1108 50-51 -0.7592749977670259 1108 52-53 -0.41013549932956295 1108 54-55 -0.31455198818472496 1108 56-57 -0.21717333406949635 1108 58-59 -1.2420328361215063 1108 60-61 -0.17100024258487778 1108 62-63 -0.2463907496365607 1108 64-65 -0.6505529437928566 1108 66-67 -0.7922958303401479 1108 68-69 -0.7358834413773678 1108 70-71 0.1909905695322287 1108 72-73 -0.3129596719477803 1108 74-75 0.5180238042485961 1108 76 -0.24644997181720996 1109 1 -0.03921429944200483 1109 2 -0.4543207479277811 1109 3 0.2244403688348413 1109 4 -0.1369588240851769 1109 5 -0.4414749925771311 1109 6 -0.33986765097667515 1109 7 -1.2204370013658483 1109 8 0.13424148299882788 1109 9 0.29967348914086145 1109 10-11 0.07751944912101294 1109 12-13 -0.2401468046224764 1109 14-15 0.4710547719029883 1109 16-17 -1.2056631259208999 1109 18-19 0.23822397405598394 1109 20-21 0.13521389641588044 1109 22-23 0.45169065979772327 1109 24-25 -0.01582248083686366 1109 26-27 -0.9541915256062801 1109 28-29 0.08020723320852241 1109 30-31 0.5474841667362398 1109 32-33 0.5716620331653246 1109 34-35 -0.4445450068278518 1109 36-37 0.11325106452351719 1109 38-39 0.26196585253020643 1109 40-41 0.14035770445186557 1109 42-43 0.5063878408084328 1109 44-45 -0.05873479540398563 1109 46-47 -1.2082782564614334 1109 48-49 0.14897964533437857 1109 50-51 0.9230108117210847 1109 52-53 -0.027383198109191653 1109 54-55 -0.1557365439763032 1109 56-57 0.04131306686348779 1109 58-59 -0.046897678472291204 1109 60-61 0.3031136313790519 1109 62-63 0.12784391236518644 1109 64-65 0.4650703641333358 1109 66-67 0.056240135339386654 1109 68-69 0.4779322110862907 1109 70-71 0.28639229388559784 1109 72-73 -0.680155306917996 1109 74-75 0.8546892213838326 1109 76 -0.4717404878736886 1110 1 -0.6963836886615411 1110 2 -0.4582884993945804 1110 3 -1.5815619105117449 1110 4 -0.6233460597442892 1110 5 -1.2077575471337134 1110 6 -1.217078406115757 1110 7 -1.9054650710969696 1110 8 -0.5753106676068853 1110 9 -1.627978361772989 1110 10-11 -1.7600560014720372 1110 12-13 -1.2111062406952442 1110 14-15 -1.3766933180889929 1110 16-17 -1.3421706604276764 1110 18-19 -1.040890457584723 1110 20-21 -1.8840128141493118 1110 22-23 -1.604617642933274 1110 24-25 -1.5109683168083308 1110 26-27 -2.1964776258131096 1110 28-29 -1.5401760346991225 1110 30-31 -1.79042896483271 1110 32-33 -0.21171562079609174 1110 34-35 -0.9536623931770976 1110 36-37 -2.1234632995809903 1110 38-39 -1.3158062972313083 1110 40-41 -1.199328252240548 1110 42-43 -1.1316814682118697 1110 44-45 -0.07274855590375395 1110 46-47 -0.15433372056172345 1110 48-49 -1.1818632908017044 1110 50-51 -0.5100861449291223 1110 52-53 -1.024829485728958 1110 54-55 -0.8787326184349595 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0.2133240754661081 2116 40-41 0.7034515088142363 2116 42-43 -0.127159596633021 2116 44-45 2.353616335333335 2116 46-47 1.177309860760566 2116 48-49 1.7757025143294314 2116 50-51 1.0289923452417575 2116 52-53 0.5228643597658298 2116 54-55 0.18321702219524738 2116 56-57 0.16669778518276956 2116 58-59 0.06978906045451083 2116 60-61 -1.6070295507432526 2116 62-63 1.1498564909815379 2116 64-65 1.413417748048765 2116 66-67 1.1525869839945457 2116 68-69 0.9636139988555996 2116 70-71 -0.972740584476508 2116 72-73 1.2542753099694615 2116 74-75 0.6551709556271099 2116 76 0.20763861021275432 2117 1 -0.9157357238119417 2117 2 0.011992993531883656 2117 3 -0.47475876242618753 2117 4 0.510930411696009 2117 5 -0.12026780738189302 2117 6 -0.17014682386477986 2117 7 -0.5595619278397628 2117 8 -0.12076960247241786 2117 9 0.5698875066061326 2117 10-11 -1.1569948262511431 2117 12-13 -0.04119653087920483 2117 14-15 -1.0838296224683077 2117 16-17 0.13117683607176645 2117 18-19 -0.4860645196773312 2117 20-21 -0.9347829363066218 2117 22-23 -0.3380926079277238 2117 24-25 -0.06656679104907326 2117 26-27 0.7278138135186474 2117 28-29 0.11348627035616232 2117 30-31 -0.11221448943316403 2117 32-33 0.5684495088451591 2117 34-35 -0.5396312020226368 2117 36-37 -1.297114982261693 2117 38-39 -0.6026104109872819 2117 40-41 0.19628277571560915 2117 42-43 -0.6960916850048768 2117 44-45 0.19179685474759367 2117 46-47 -0.5170878125901126 2117 48-49 0.3101940700210477 2117 50-51 0.34701877516165425 2117 52-53 0.8881636097275596 2117 54-55 -0.3100734518085275 2117 56-57 0.9903877693656362 2117 58-59 -0.6955561102174599 2117 60-61 0.47233674345542553 2117 62-63 -0.3381531013446022 2117 64-65 -0.3777195548748544 2117 66-67 -0.6421219297255405 2117 68-69 0.8810587664457792 2117 70-71 0.30247753889713636 2117 72-73 1.0221206081989642 2117 74-75 0.2137453726892069 2117 76 -0.007372359734691969 2118 1 1.100885461998029 2118 2 0.37721254203101395 2118 3 1.120081227650715 2118 4 0.2240040906838523 2118 5 0.3140661047987088 2118 6 1.4891932902508245 2118 7 2.465791582455445 2118 8 -1.7623969902173933 2118 9 1.0837550332744534 2118 10-11 -0.4150201301459546 2118 12-13 -0.27629948300026896 2118 14-15 -0.48025730790616805 2118 16-17 0.462781896850732 2118 18-19 -0.5288330634629936 2118 20-21 -1.0800322540937692 2118 22-23 -0.12310873295873037 2118 24-25 0.9221953027241838 2118 26-27 0.4455265398892756 2118 28-29 -0.3571165577261972 2118 30-31 0.5392863788377369 2118 32-33 0.7888166633973768 2118 34-35 1.239579911844892 2118 36-37 0.5851226758876464 2118 38-39 1.1880122632870922 2118 40-41 -0.4483215408311523 2118 42-43 0.1325860624523294 2118 44-45 1.09834609811152 2118 46-47 0.14656884828808003 2118 48-49 -0.11456405451380647 2118 50-51 -0.011775568673623837 2118 52-53 -0.487847305678045 2118 54-55 0.6082842758024825 2118 56-57 0.9219680224045845 2118 58-59 1.040588766984225 2118 60-61 0.16149576106892383 2118 62-63 -0.48347684235179145 2118 64-65 0.7647016805505977 2118 66-67 -0.9582469607352166 2118 68-69 0.41844403064821023 2118 70-71 -0.1555725727376931 2118 72-73 0.3614408761225576 2118 74-75 1.4566627629149558 2118 76 0.1371079181119974 2119 1 0.01351185556816148 2119 2 1.5611897263711754 2119 3 0.9368818498959861 2119 4 0.35006061129572785 2119 5 1.0227516116693351 2119 6 1.0433545554828711 2119 7 0.901518831612826 2119 8 2.074989579655565 2119 9 0.2953702782835279 2119 10-11 0.6581455426567402 2119 12-13 1.237675108651274 2119 14-15 0.3408471774451449 2119 16-17 0.17190816078643323 2119 18-19 1.0079312611416213 2119 20-21 1.3626693274462873 2119 22-23 1.4275264757527033 2119 24-25 1.5547076179951205 2119 26-27 0.9528638486784864 2119 28-29 0.9611359701452038 2119 30-31 2.1098334361361566 2119 32-33 1.4936131373408585 2119 34-35 1.5732589369939696 2119 36-37 -0.08978009828917521 2119 38-39 1.1858084779617108 2119 40-41 0.9065656772139583 2119 42-43 0.46061302641395585 2119 44-45 0.004205574388421951 2119 46-47 1.0150158278058399 2119 48-49 0.4762837546643084 2119 50-51 1.3885614808467288 2119 52-53 0.1928371641171296 2119 54-55 -0.07068901950474782 2119 56-57 1.4973115536464832 2119 58-59 1.8853385725325786 2119 60-61 0.6946821753805636 2119 62-63 0.7826885711820424 2119 64-65 1.279039248608676 2119 66-67 0.9017737807737305 2119 68-69 0.005713929533353479 2119 70-71 0.433434427521572 2119 72-73 -1.0621007365297643 2119 74-75 -0.5022171956020252 2119 76 1.7148107959963639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 28.0 13 248.0 14 775.0 15 1590.0 16 2093.0 17 2377.0 18 2511.0 19 2409.0 20 1978.0 21 1618.0 22 1209.0 23 888.0 24 814.0 25 724.0 26 693.0 27 768.0 28 847.0 29 953.0 30 1134.0 31 1365.0 32 1775.0 33 2309.0 34 3290.0 35 4949.0 36 8135.0 37 9867.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.330081715107287 32.445811418275795 9.076018708937704 29.14808815767922 2 14.809394760614273 23.219512195121954 30.426377597109305 31.54471544715447 3 13.441734417344172 33.81210478771454 26.07588075880759 26.670280036133693 4 9.824751580849142 30.397470641373083 30.4643179765131 29.31345980126468 5 9.495934959349594 42.20415537488708 29.63324299909666 18.666666666666668 6 19.582655826558266 37.69828364950316 15.226738934056009 27.492321589882568 7 22.303523035230352 34.60343270099367 18.48780487804878 24.60523938572719 8 20.489611562782294 35.96205962059621 17.9223125564589 25.626016260162604 9 20.303523035230352 21.868112014453477 18.169828364950316 39.65853658536586 10-11 19.418247515808492 27.900632339656728 25.6196928635953 27.061427280939476 12-13 18.59981933152665 25.848238482384822 25.31797651309847 30.233965672990067 14-15 18.752484191508582 27.595302619692863 22.332429990966578 31.31978319783198 16-17 20.4643179765131 28.853658536585364 23.52393857271906 27.158084914182474 18-19 18.965672990063233 27.589882565492324 23.761517615176153 29.68292682926829 20-21 17.853658536585364 28.102981029810298 24.08130081300813 29.962059620596204 22-23 21.169828364950316 27.422764227642276 23.057813911472447 28.34959349593496 24-25 19.476964769647697 30.598012646793133 22.645889792231255 27.279132791327914 26-27 18.018970189701896 30.593495934959346 24.77777777777778 26.609756097560975 28-29 20.037037037037038 28.004516711833784 26.75609756097561 25.20234869015357 30-31 19.675700090334235 28.279132791327914 26.498644986449865 25.546522131887983 32-33 17.70731707317073 30.89250225835592 25.591689250225834 25.808491418247513 34-35 18.56007226738934 29.400180668473354 25.365853658536587 26.673893405600722 36-37 18.919602529358627 28.0063233965673 26.163504968383016 26.910569105691057 38-39 18.486901535682023 26.831978319783197 27.724480578139115 26.956639566395662 40-41 17.76151761517615 26.621499548328813 27.327913279132794 28.28906955736224 42-43 16.23848238482385 26.425474254742547 28.619692863595304 28.7163504968383 44-45 16.493224932249323 27.145438121047878 28.947606142728095 27.413730803974705 46-47 17.140018066847336 27.959349593495936 27.56910569105691 27.33152664859982 48-49 16.313459801264678 28.897922312556457 26.581752484191508 28.20686540198735 50-51 16.197831978319783 28.80307136404697 25.94850948509485 29.050587172538396 52-53 15.858175248419151 28.14724480578139 26.682926829268293 29.31165311653117 54-55 15.240289069557361 28.40650406504065 26.144534778681123 30.208672086720867 56-57 16.552845528455286 28.267389340560072 25.57091237579042 29.60885275519422 58-59 16.77326106594399 28.060523938572718 25.422764227642276 29.74345076784101 60-61 15.928635953026197 27.28906955736224 25.31165311653116 31.470641373080397 62-63 16.493224932249323 27.354110207768745 25.164408310749774 30.988256549232158 64-65 17.23486901535682 28.161698283649507 23.479674796747968 31.123757904245707 66-67 16.357723577235774 29.572719060523937 23.088527551942185 30.981029810298104 68-69 17.859078590785906 29.27913279132791 23.064137308039747 29.79765130984643 70-71 18.920505871725386 29.157181571815716 23.04155374887082 28.880758807588077 72-73 19.1806684733514 29.401987353206867 22.705510388437215 28.711833785004515 74-75 18.28816621499548 30.049683830171638 22.522131887985548 29.140018066847333 76 18.847335140018068 28.5420054200542 23.315266485998194 29.29539295392954 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.0 9 0.5 10 0.5 11 1.0 12 1.0 13 1.5 14 5.0 15 10.0 16 13.5 17 15.0 18 13.0 19 15.0 20 26.5 21 34.0 22 42.0 23 49.0 24 71.5 25 95.0 26 122.0 27 189.5 28 263.5 29 297.0 30 357.5 31 480.5 32 672.0 33 801.0 34 886.0 35 1144.5 36 1440.5 37 1563.0 38 1799.5 39 2257.5 40 2690.0 41 3025.5 42 3150.0 43 3377.5 44 3727.0 45 3913.5 46 3978.0 47 4000.0 48 3920.5 49 3619.5 50 3420.0 51 3243.5 52 2821.5 53 2363.0 54 2150.0 55 2033.0 56 1707.5 57 1297.5 58 1096.0 59 994.0 60 754.0 61 519.0 62 422.0 63 379.0 64 299.0 65 220.5 66 149.0 67 119.0 68 103.5 69 75.5 70 47.5 71 32.0 72 31.0 73 27.5 74 21.5 75 18.0 76 13.0 77 10.0 78 10.0 79 8.0 80 8.0 81 7.0 82 6.0 83 6.0 84 8.0 85 7.5 86 5.5 87 6.0 88 6.0 89 5.0 90 4.5 91 5.0 92 5.0 93 4.5 94 4.5 95 3.5 96 2.0 97 3.5 98 7.0 99 6.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7280939476061428 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 55350.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.3423667570009 #Duplication Level Percentage of deduplicated Percentage of total 1 84.6050549134334 63.74345076784101 2 8.097932952855977 12.202348690153569 3 3.4242961968250927 7.739837398373983 4 1.6090355378638912 4.849141824751581 5 0.892043547072083 3.360433604336043 6 0.5179607692676611 2.341463414634146 7 0.29494988249964027 1.5555555555555556 8 0.1822454558534363 1.098464317976513 9 0.1462759579876265 0.991869918699187 >10 0.23020478634118266 2.1174345076784102 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005420054200542005 0.0 2 0.0 0.0 0.0 0.005420054200542005 0.0 3 0.0 0.0 0.0 0.005420054200542005 0.0 4 0.0 0.0 0.0 0.007226738934056007 0.0 5 0.0 0.0 0.0 0.007226738934056007 0.0 6 0.0 0.0 0.0 0.007226738934056007 0.0 7 0.0 0.0 0.0 0.007226738934056007 0.0 8 0.0 0.0 0.0 0.007226738934056007 0.0 9 0.0 0.0 0.0 0.014453477868112014 0.0 10 0.0 0.0 0.0 0.016260162601626018 0.0 11 0.0 0.0 0.0 0.016260162601626018 0.0 12 0.0 0.0 0.0 0.018066847335140017 0.0 13 0.0 0.0 0.0 0.01987353206865402 0.0 14 0.0 0.0 0.0 0.023486901535682024 0.0 15 0.0 0.0 0.0 0.025293586269196026 0.0 16 0.0 0.0 0.0 0.028906955736224028 0.0 17 0.0 0.0 0.0 0.032520325203252036 0.0 18 0.0 0.0 0.0 0.037940379403794036 0.0 19 0.0 0.0 0.0 0.04878048780487805 0.0 20 0.0 0.0 0.0 0.05058717253839205 0.0 21 0.0 0.0 0.0 0.05600722673893405 0.0 22 0.0 0.0 0.0 0.06142728093947606 0.0 23 0.0 0.0 0.0 0.06684733514001806 0.0 24 0.0 0.0 0.0 0.07226738934056007 0.0 25 0.0 0.0 0.0 0.07226738934056007 0.0 26 0.0 0.0 0.0 0.07407407407407407 0.0 27 0.0 0.0 0.0 0.0921409214092141 0.0 28 0.0 0.0 0.0 0.1120144534778681 0.0 29 0.0 0.0 0.0 0.12285456187895212 0.0 30 0.0 0.0 0.0 0.14995483288166214 0.0 31 0.0 0.0 0.0 0.1933152664859982 0.0 32 0.0 0.0 0.0 0.2186088527551942 0.0 33 0.0 0.0 0.0 0.26919602529358627 0.0 34 0.0 0.0 0.0 0.3215898825654923 0.0 35 0.0 0.0 0.0 0.4209575429087624 0.0 36 0.0 0.0 0.0 0.5293586269196026 0.0 37 0.0 0.0 0.0 0.7461607949412827 0.0 38 0.0 0.0 0.0 1.008130081300813 0.0 39 0.0 0.0 0.0 1.3387533875338753 0.0 40 0.0 0.0 0.0 1.7795844625112918 0.0 41 0.0 0.0 0.0 2.15356820234869 0.0 42 0.0 0.0 0.0 2.607046070460705 0.0 43 0.0 0.0 0.0 3.138211382113821 0.0 44 0.0 0.0 0.0 3.6169828364950316 0.0 45 0.0 0.0 0.0 4.182475158084914 0.0 46 0.0 0.0 0.0 4.7046070460704605 0.0 47 0.0 0.0 0.0 5.1833785004516715 0.0 48 0.0 0.0 0.0 5.682023486901536 0.0 49 0.0 0.0 0.0 6.146341463414634 0.0 50 0.0 0.0 0.0 6.688346883468835 0.0 51 0.0 0.0 0.0 7.186991869918699 0.0 52 0.0 0.0 0.0 7.687443541102078 0.0 53 0.0 0.0 0.0 8.184281842818429 0.0 54 0.0 0.0 0.0 8.719060523938573 0.0 55 0.0 0.0 0.0 9.112917795844625 0.0 56 0.0 0.0 0.0 9.649503161698284 0.0 57 0.0 0.0 0.0 10.075880758807589 0.0 58 0.0 0.0 0.0 10.484191508581752 0.0 59 0.0 0.0 0.0 10.899728997289973 0.0 60 0.0 0.0 0.0 11.268292682926829 0.0 61 0.0 0.0 0.0 11.709123757904246 0.0 62 0.0 0.0 0.0 12.202348690153569 0.0 63 0.0 0.0 0.0 12.654019873532068 0.0 64 0.0 0.0 0.0 13.022583559168925 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGAACT 15 0.002215176 69.98826 49 GTGCTTC 15 0.002215176 69.98826 37 GAAACGT 15 0.002215176 69.98826 28 ACAGCTT 15 0.002215176 69.98826 10 CCTGGCG 25 2.3731064E-4 55.9906 30 ATAGGTG 20 0.006921893 52.491196 5 TGGCGGT 20 0.006921893 52.491196 32 TTCACAG 20 0.006921893 52.491196 39 AGAACTA 20 0.006921893 52.491196 50 ATCGTAC 20 0.006921893 52.491196 10 GGAAGGT 20 0.006921893 52.491196 14 GCTTCAT 20 0.006921893 52.491196 39 TACGAGC 20 0.006921893 52.491196 2 GGCGGTG 20 0.006921893 52.491196 33 ACATGAG 20 0.006921893 52.491196 17 TTACGCT 20 0.006921893 52.491196 67 AAGGACC 20 0.006921893 52.491196 25 CTGGCGG 20 0.006921893 52.491196 31 GATAGGT 20 0.006921893 52.491196 4 ACGCGCA 20 0.006921893 52.491196 12 >>END_MODULE