FastQCFastQC Report
Wed 25 May 2016
SRR1780502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55350
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT39107.064137308039747No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT35416.39747064137308Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC13172.3794037940379402No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC6861.2393857271906052No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG4080.7371273712737128No Hit
TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT3330.6016260162601627No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1690.3053297199638663No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1600.2890695573622403No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT1570.2836495031616983No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1550.2800361336946703No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1360.24570912375790427No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1270.22944896115627825No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT1200.21680216802168023No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCTT1130.20415537488708219No Hit
CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC990.17886178861788618No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGT990.17886178861788618No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGTCTT930.16802168021680217No Hit
CTTATCACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC840.15176151761517614No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTCTT740.13369467028003615No Hit
TTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC700.1264679313459801No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG660.11924119241192412No Hit
CTTATTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG630.11382113821138211No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTTTGTATCTCGTATGCCGT620.11201445347786812No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACT150.00221370270.0000159
ATAGGAC150.00221370270.000018
GAATCTC150.00221370270.0000132
AATCTCG150.00221370270.0000133
CACGTAT150.00221370270.0000139
CGTTTGA150.00221370270.0000127
TCACGTA150.00221370270.0000138
CTTATAA352.9241528E-760.01
CTTTACA1750.060.01
TTTACAC1800.058.3333362
ACGTATG252.37114E-455.99999640
CGGCGGG200.00691730452.58
TTGGCCA200.00691730452.517
AGATCCG200.00691730452.522
CCGGCGG200.00691730452.57
TGTATCA200.00691730452.534
TTGTACG200.00691730452.59
TATGCTG200.00691730452.540
ATCACGT200.00691730452.537
ATCCGTT200.00691730452.524
Produced by FastQC (version 0.11.5)