FastQCFastQC Report
Wed 25 May 2016
SRR1780501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325449
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATTCG300.005716971629.3563340
GGCACTT300.005738520529.3337421
TCACTGA300.005768797429.30218327
TACACGT300.00577313329.297682
CTTATGC406.988901E-427.52155937
TACAGAC406.988901E-427.52155938
GAGTTCG407.077458E-427.4623553
TCTGACC508.2614155E-526.42883511
CGGTGGT1400.025.11615843
CTTTACA2000.024.2003381
CGACAAT651.7928855E-523.71818517
TCGGTGG2150.023.5098942
TTTACAC2150.023.5062772
CTTATAA852.3413486E-723.2944431
ACGCGGC1052.9885996E-923.08701715
CTATACA1053.0486262E-923.0479411
TCTGTCG500.002560132222.02402911
TATCTTC500.002571606322.0070823
GTCATAC602.8662517E-422.0003071
GTACAAG703.2056876E-522.0003071
Produced by FastQC (version 0.11.5)