FastQCFastQC Report
Wed 25 May 2016
SRR1780501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325449
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT304459.354768335438118No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT248137.624236055418822RNA PCR Primer, Index 20 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC81242.496243651078971No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC44631.3713362155053481No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG35581.093258851617304No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT22180.6815199923797585No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC16420.504533736468694No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC12730.39115191627566837No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG11460.35212890498972194No Hit
CTTATAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG9660.29682070001751426No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC9410.2891390048824854No Hit
CTTAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC7140.2193892130564236No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG5530.16991909638683786No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC4840.1487176178141583No Hit
CTTATCACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC4720.14503040414934446No Hit
CTTATTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG4240.13028154949008908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACATT207.85126E-443.99646441
CCGTCTT26300.042.40723844
TTTACAC9400.038.8479462
CTTTACA9650.038.0811841
GCCGTCT29450.037.87132343
CTATACA5850.036.110761
GCGTTGA250.002345887235.21340615
TGGCGTG505.927359E-835.19717423
TACTGTC250.002351201135.19717441
ATTGGCG505.927359E-835.19717421
GCGTGAA505.927359E-835.19717425
TTACACA10950.033.7507133
CTTATAC43400.032.449761
TTATACA43600.032.1900752
TGAAGGT551.3694626E-731.99742928
TGGCGGA353.2142494E-431.42604627
CGAGGCT353.2142494E-431.42604631
GCGGATA353.2142494E-431.42604629
GAACCAT353.2142494E-431.42604622
TGGGATG353.2142494E-431.42604622