FastQCFastQC Report
Wed 25 May 2016
SRR1780500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59457
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATG150.002215251869.994112
ACAGCAT150.002215251869.9941155
GAATTCG150.002215251869.9941147
GATTCCG150.002215251869.9941168
TTCGAAC150.002215251869.9941150
CCCGATT150.002215251869.9941165
GATGTTT200.00692211952.49558652
CTTCCGA200.00692211952.49558618
ATTCCGC200.00692211952.49558669
AATTCGA200.00692211952.49558648
GACGCTT200.00692211952.49558614
GCTCCAG200.00692211952.49558612
TACAAGA200.00692211952.49558627
TCTATGT200.00692211952.4955863
TCGCCGT305.8367033E-446.6627449
GACCCTA350.001246705239.99663518
TGGTCGC350.001246705239.99663546
CGACGAG400.0024020834.99705519
TTATGAA400.0024020834.99705543
GGTCGCC552.961142E-431.81550447