##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780500_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59457 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.977126326588962 33.0 23.0 34.0 12.0 34.0 2 29.023798711673983 34.0 23.0 34.0 12.0 34.0 3 28.820071648418185 34.0 23.0 34.0 11.0 34.0 4 29.463898279428832 34.0 31.0 34.0 11.0 34.0 5 29.342936912390467 34.0 27.0 34.0 11.0 34.0 6 31.92675378845216 37.0 28.0 38.0 11.0 38.0 7 31.206182619371983 37.0 27.0 38.0 11.0 38.0 8 30.721681214995712 36.0 26.0 38.0 11.0 38.0 9 31.163782229174025 37.0 27.0 38.0 12.0 38.0 10-11 31.307230435440736 37.0 27.0 38.0 11.0 38.0 12-13 31.54729468355282 37.0 27.0 38.0 11.0 38.0 14-15 31.16194897152564 37.0 27.0 38.0 11.0 38.0 16-17 30.958364868728662 37.0 26.0 38.0 11.0 38.0 18-19 31.04752173839918 37.0 26.5 38.0 11.0 38.0 20-21 31.10296516810468 37.0 26.5 38.0 11.0 38.0 22-23 30.99434044771852 37.0 26.0 38.0 11.0 38.0 24-25 31.388423566611166 37.0 27.0 38.0 11.0 38.0 26-27 31.357047950619776 37.0 27.0 38.0 11.0 38.0 28-29 31.33324082950704 37.0 27.0 38.0 11.0 38.0 30-31 31.191323141093562 37.0 26.0 38.0 11.0 38.0 32-33 31.10124964259885 37.0 26.5 38.0 11.0 38.0 34-35 31.046966715441414 37.0 26.0 38.0 11.0 38.0 36-37 30.997586491077584 37.0 26.0 38.0 11.0 38.0 38-39 31.288334426560372 37.0 27.0 38.0 11.0 38.0 40-41 30.91207931782633 37.0 26.0 38.0 11.0 38.0 42-43 31.270918478900718 37.0 26.5 38.0 11.0 38.0 44-45 30.427510637940024 36.0 25.0 38.0 11.0 38.0 46-47 31.042072422086548 37.0 26.0 38.0 11.0 38.0 48-49 30.900457473468222 37.0 26.0 38.0 11.0 38.0 50-51 30.886497805136486 36.5 26.0 38.0 11.0 38.0 52-53 30.87413593016802 37.0 26.0 38.0 11.0 38.0 54-55 30.66146963351666 36.5 25.5 38.0 11.0 38.0 56-57 30.65100829170661 36.5 25.5 38.0 11.0 38.0 58-59 30.50159779336327 36.5 25.5 38.0 11.0 38.0 60-61 30.61353583261853 36.5 25.5 38.0 11.0 38.0 62-63 30.59654876633533 36.5 25.0 38.0 11.0 38.0 64-65 30.642270884841146 37.0 25.0 38.0 11.0 38.0 66-67 30.644280740703365 36.5 25.0 38.0 11.0 38.0 68-69 30.139806919286208 36.0 24.5 38.0 11.0 38.0 70-71 29.942311250147164 36.0 24.0 38.0 11.0 38.0 72-73 29.951553223337875 36.0 24.0 38.0 11.0 38.0 74-75 29.70207881326 35.5 23.5 38.0 11.0 38.0 76 28.94986292614831 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.8952092284261717 1101 2 -1.8883602760601264 1101 3 -1.8606397975446818 1101 4 -3.5634125886114774 1101 5 -1.921101699195706 1101 6 -2.9125470607491266 1101 7 -1.9543623577183666 1101 8 -2.5705787408678127 1101 9 -0.9012598526499751 1101 10-11 -1.1741568099220494 1101 12-13 -1.9325175923078213 1101 14-15 -1.19778189713249 1101 16-17 -1.6336480633559667 1101 18-19 -1.618209356520211 1101 20-21 -2.392856432116897 1101 22-23 -1.505775029031863 1101 24-25 -2.6697630531725487 1101 26-27 -2.639211624547194 1101 28-29 -1.293628548036608 1101 30-31 -2.0161657448201034 1101 32-33 -1.999827400910469 1101 34-35 -2.7157206887979584 1101 36-37 -1.0988011202998464 1101 38-39 -1.8142756583669346 1101 40-41 -1.4982481389246374 1101 42-43 -1.340620974851653 1101 44-45 -1.081622883343961 1101 46-47 -1.0455727254124838 1101 48-49 -2.5720380202817665 1101 50-51 -2.2286277367296066 1101 52-53 -1.3036939390646367 1101 54-55 -2.414587362151476 1101 56-57 -2.480788932185714 1101 58-59 -1.565795874125918 1101 60-61 -1.5358647494247357 1101 62-63 -2.052960284409888 1101 64-65 -1.5582979644566741 1101 66-67 -2.636810876966411 1101 68-69 -0.5250060749594248 1101 70-71 -1.2139221154580895 1101 72-73 -1.036193562588604 1101 74-75 -2.2965077321714205 1101 76 -2.9872258192610204 1102 1 1.6274585799559205 1102 2 0.7208400474259093 1102 3 1.1720418065654918 1102 4 1.775962322192008 1102 5 2.1876466952669738 1102 6 1.212518350030571 1102 7 1.618683643899061 1102 8 2.9437640591892773 1102 9 2.2111039739995633 1102 10-11 1.4068574139493215 1102 12-13 -2.2746754344656637 1102 14-15 0.4094442100936142 1102 16-17 -0.7410644564866438 1102 18-19 0.6520204137095575 1102 20-21 1.8979004300685425 1102 22-23 1.1395320448466428 1102 24-25 1.7412782864402203 1102 26-27 -4.561884190076903 1102 28-29 1.3698984012046296 1102 30-31 2.1283563997020387 1102 32-33 -4.934749621546974 1102 34-35 1.0300573426943593 1102 36-37 2.266476459263444 1102 38-39 2.0407740538256363 1102 40-41 1.62175090964584 1102 42-43 0.9381003038696321 1102 44-45 -4.263488636638286 1102 46-47 1.645355394087069 1102 48-49 0.5385180902743443 1102 50-51 -5.4071397009558595 1102 52-53 -0.7379501590351403 1102 54-55 1.1505379886881606 1102 56-57 -0.36718824715645937 1102 58-59 2.434846340802011 1102 60-61 1.752656253381982 1102 62-63 -3.1943189257685276 1102 64-65 0.180415702828423 1102 66-67 0.523790426492031 1102 68-69 -5.660989139513916 1102 70-71 0.5036936021576253 1102 72-73 1.3293694458315493 1102 74-75 -5.249269256361515 1102 76 -1.6671649277715552 1103 1 -0.7290747848350811 1103 2 0.0806764675430891 1103 3 0.20023574309964332 1103 4 -0.16941929866442962 1103 5 0.3326272308338005 1103 6 -2.141537723265799 1103 7 -0.030759948566824846 1103 8 1.0136000860855816 1103 9 0.11539401371487301 1103 10-11 -0.05382049883429474 1103 12-13 -1.1867315864062071 1103 14-15 -1.1445056935672717 1103 16-17 -0.5407380831627471 1103 18-19 -0.43798784392875945 1103 20-21 -0.25667469477908966 1103 22-23 -0.297965497522906 1103 24-25 -0.014138331073837662 1103 26-27 -0.9050873673015509 1103 28-29 -0.3338686547507024 1103 30-31 -0.2801214423018088 1103 32-33 -4.746947333001501 1103 34-35 -1.4051988407038571 1103 36-37 -1.5122778170135405 1103 38-39 0.10591109129958554 1103 40-41 0.1783353931502205 1103 42-43 -0.17131340380997884 1103 44-45 -3.888021686852589 1103 46-47 0.019446070820187344 1103 48-49 -0.9830893966510637 1103 50-51 -0.6538268151078768 1103 52-53 -0.7893441270269612 1103 54-55 -0.26834054538442587 1103 56-57 0.6680246819458198 1103 58-59 0.12182286551594501 1103 60-61 0.4704612944735622 1103 62-63 0.14878594623807828 1103 64-65 0.5265680365942984 1103 66-67 -0.8792216453887427 1103 68-69 -2.5606784950249057 1103 70-71 -0.5510820653348034 1103 72-73 0.4567730084126502 1103 74-75 -1.5995110871978966 1103 76 -0.3405949934394421 1104 1 -2.551202358103513 1104 2 0.2979972260868493 1104 3 -0.09672534941971023 1104 4 0.8764516407264367 1104 5 0.2713082300563272 1104 6 -0.04884935494615661 1104 7 1.0670665058157596 1104 8 0.9338583425166398 1104 9 -0.36545647474133247 1104 10-11 0.19346679454365656 1104 12-13 -0.052870923187466445 1104 14-15 0.22230970716963938 1104 16-17 0.009442763945390453 1104 18-19 0.867559260827349 1104 20-21 0.8392418789287852 1104 22-23 0.9534119827867293 1104 24-25 0.2510646571288433 1104 26-27 1.5103201302477167 1104 28-29 0.24888157342088135 1104 30-31 -0.08801786930380473 1104 32-33 -0.47956403854184515 1104 34-35 0.5117974071411915 1104 36-37 0.06710899297868522 1104 38-39 0.39535097231232896 1104 40-41 1.282330931128115 1104 42-43 0.6874737374535869 1104 44-45 -2.3923822993665347 1104 46-47 0.10919356093275212 1104 48-49 0.8593200952868756 1104 50-51 1.1533090399440127 1104 52-53 0.6240858834520289 1104 54-55 0.17028974904142302 1104 56-57 0.4952058332827356 1104 58-59 0.8854371034228521 1104 60-61 0.93716514465779 1104 62-63 0.9005014585255395 1104 64-65 0.8635521152881438 1104 66-67 0.45242152805546354 1104 68-69 -2.8754538727756405 1104 70-71 -0.21435151062432922 1104 72-73 1.511551090419207 1104 74-75 0.38833666319928994 1104 76 1.219456387421186 1105 1 -0.8819186515996833 1105 2 -0.12179534927420832 1105 3 -0.6388726144515324 1105 4 -0.4753625171359914 1105 5 0.11683826139618958 1105 6 -0.06086835271682389 1105 7 0.942094776307826 1105 8 0.35914347062235663 1105 9 -0.12271795256111417 1105 10-11 -0.18169071248357582 1105 12-13 -1.0323707777963662 1105 14-15 -0.64672521900399 1105 16-17 0.9022249034562257 1105 18-19 0.7913258596448358 1105 20-21 0.19024830844641016 1105 22-23 0.16091739456663845 1105 24-25 0.5913929179281752 1105 26-27 0.6797815369874023 1105 28-29 -0.34547725181901257 1105 30-31 0.22043743100959645 1105 32-33 -0.1330808877551135 1105 34-35 -0.027991291123807827 1105 36-37 0.42252561925893417 1105 38-39 0.35189518736192227 1105 40-41 0.23284949272547806 1105 42-43 0.5557009352824096 1105 44-45 1.180943279774688 1105 46-47 0.7742124617760204 1105 48-49 -0.2245705706043708 1105 50-51 -0.14046175906086944 1105 52-53 -0.5063945533674605 1105 54-55 -0.08338541492742735 1105 56-57 -0.2175659673052941 1105 58-59 0.19744640845338068 1105 60-61 -0.033546625559647936 1105 62-63 -0.16432681842835706 1105 64-65 -0.473415995596806 1105 66-67 0.077441721264087 1105 68-69 0.8249919932706895 1105 70-71 1.2178477721129006 1105 72-73 0.25252876480775654 1105 74-75 -0.5204384975386489 1105 76 -0.6725018691779532 1106 1 0.00476821348675216 1106 2 1.4707193539872456 1106 3 0.4065381857623329 1106 4 -0.1591265520673737 1106 5 0.77540179730779 1106 6 0.7142519466950432 1106 7 1.9405620726899357 1106 8 1.667003032197833 1106 9 1.470046260591623 1106 10-11 1.2273094657377719 1106 12-13 0.8649559019398687 1106 14-15 0.8685839950398524 1106 16-17 1.1994446503819098 1106 18-19 0.521605667165776 1106 20-21 1.0440711564235947 1106 22-23 1.3689373553579394 1106 24-25 1.25455456469345 1106 26-27 1.0033780438489224 1106 28-29 -0.10578735696960706 1106 30-31 0.6627650018525806 1106 32-33 1.8272648616656681 1106 34-35 1.5448477749963097 1106 36-37 0.8888596060705538 1106 38-39 0.5700915872906336 1106 40-41 0.568974960556524 1106 42-43 1.3007731149295338 1106 44-45 1.6880364895412185 1106 46-47 1.0750679242604306 1106 48-49 1.1554151716875545 1106 50-51 1.418315642471832 1106 52-53 0.1575512673418622 1106 54-55 0.8416725070116193 1106 56-57 0.606939468445912 1106 58-59 0.10699907286257826 1106 60-61 1.0914756521092137 1106 62-63 1.4642064198535962 1106 64-65 1.5595905985931786 1106 66-67 1.6717386380347143 1106 68-69 2.23648727040489 1106 70-71 1.298778087564692 1106 72-73 0.9458276420132457 1106 74-75 0.11208689900385593 1106 76 0.25888291071867897 1107 1 0.7661294329124892 1107 2 0.5986527109528588 1107 3 0.672826735921852 1107 4 1.0844395592406713 1107 5 0.4545226764286703 1107 6 -0.22573387393594047 1107 7 -0.1531635565195657 1107 8 0.2985521282630579 1107 9 0.7281107767206549 1107 10-11 0.12729174152650202 1107 12-13 0.15389599410576338 1107 14-15 -0.3616180609686559 1107 16-17 0.9764917967838933 1107 18-19 0.7674050290941707 1107 20-21 0.7402604509999868 1107 22-23 -0.4116191848760167 1107 24-25 -0.30712569053518735 1107 26-27 0.5665825812470153 1107 28-29 0.20362419921614006 1107 30-31 0.23458350592914812 1107 32-33 0.790132639049574 1107 34-35 0.5899736168963479 1107 36-37 -0.30809182959055903 1107 38-39 0.2818781877869121 1107 40-41 0.3801234894469907 1107 42-43 0.4930453588146797 1107 44-45 1.069975518254438 1107 46-47 0.35977192993217244 1107 48-49 0.8178221195783735 1107 50-51 0.7838597757579038 1107 52-53 0.8678844825137872 1107 54-55 0.6710769735128608 1107 56-57 1.009970308573525 1107 58-59 1.0828055244754822 1107 60-61 0.3667326531726687 1107 62-63 0.0913953599457571 1107 64-65 0.22816557200686205 1107 66-67 0.9136386734831383 1107 68-69 0.910308610355262 1107 70-71 -0.5228631743991485 1107 72-73 -0.14433542073046013 1107 74-75 0.29939636373267575 1107 76 0.15962732759212628 1108 1 -0.4863002183610945 1108 2 -0.17365879257251748 1108 3 -0.3026544303107599 1108 4 -0.8515002235199205 1108 5 0.0031474620476750204 1108 6 0.0781154559249515 1108 7 0.3623036352482032 1108 8 0.9500162710566791 1108 9 -0.15628228686315992 1108 10-11 0.0820176514547235 1108 12-13 0.2699158124377199 1108 14-15 0.5895983526755053 1108 16-17 -0.9742640947234413 1108 18-19 -0.17209189017153648 1108 20-21 5.507387469201319E-4 1108 22-23 -0.8008556709333128 1108 24-25 -0.5256990247154576 1108 26-27 0.10936349975047577 1108 28-29 0.041853888601899314 1108 30-31 0.413520176195302 1108 32-33 0.20391971902162354 1108 34-35 0.6121421997585692 1108 36-37 0.4212481945471538 1108 38-39 1.3602463514151921 1108 40-41 1.20434695384386 1108 42-43 1.369149023935691 1108 44-45 0.5686257119913378 1108 46-47 0.299792891629437 1108 48-49 0.3261591582622323 1108 50-51 0.34906335830252644 1108 52-53 0.42452870534297915 1108 54-55 0.41085020606065825 1108 56-57 0.5726489594024642 1108 58-59 -0.9706626836170003 1108 60-61 0.9878023349359886 1108 62-63 0.07364143756755581 1108 64-65 0.7317703487330256 1108 66-67 1.0658650598135715 1108 68-69 1.2601307535300066 1108 70-71 1.9807098421796425 1108 72-73 1.3122643378924188 1108 74-75 1.4120496064437233 1108 76 1.25478072910391 1109 1 -0.8732137820121224 1109 2 -2.4188530391110312 1109 3 -1.5026141023096855 1109 4 -0.6902218258858284 1109 5 -0.5641330864131398 1109 6 -0.7640676331999323 1109 7 -2.546084588975482 1109 8 -0.023132001560608018 1109 9 -0.7777031767677158 1109 10-11 -0.4439771059051374 1109 12-13 -0.9515857334689848 1109 14-15 -1.1758271077358415 1109 16-17 -1.8312005109084133 1109 18-19 -0.791916463366853 1109 20-21 -0.46335570029058104 1109 22-23 -0.4884144826015486 1109 24-25 -0.3634124270544845 1109 26-27 -0.7131260821825798 1109 28-29 -1.539827116909592 1109 30-31 -1.2107908871196607 1109 32-33 -0.6721724976741683 1109 34-35 -1.0089594268041395 1109 36-37 -1.3231058853022901 1109 38-39 -0.9522452469179186 1109 40-41 -0.5563063597735578 1109 42-43 -0.7226139818446597 1109 44-45 -0.7739102471429504 1109 46-47 -1.3545457291529281 1109 48-49 -1.793984678274935 1109 50-51 -0.13777963134661775 1109 52-53 -0.4283076973993225 1109 54-55 -0.23925397460878628 1109 56-57 -0.46584747829077244 1109 58-59 -0.7078921499431203 1109 60-61 -0.6420202218592799 1109 62-63 -1.643239191549263 1109 64-65 -1.1082451103340496 1109 66-67 -1.0893641376592065 1109 68-69 -0.4729650926858824 1109 70-71 -0.9171258884293039 1109 72-73 -1.2296599798922294 1109 74-75 0.31450625384256625 1109 76 -1.8994066616744192 1110 1 0.27014980278651635 1110 2 -0.5795166806311656 1110 3 -0.3852006025932937 1110 4 -0.2865065678030163 1110 5 -0.45508600757025874 1110 6 -0.8163932145952799 1110 7 -0.0412632616924391 1110 8 0.6973728197666773 1110 9 -1.582864833035444 1110 10-11 -0.6542011558200542 1110 12-13 -1.0129158525841326 1110 14-15 -1.098685871207195 1110 16-17 -0.1269322182254875 1110 18-19 -0.6659238371615217 1110 20-21 0.0028241068563268357 1110 22-23 -0.8730571371676277 1110 24-25 -0.548749919964294 1110 26-27 -1.2547454809347123 1110 28-29 -0.7016960902504898 1110 30-31 -0.47631839704592593 1110 32-33 0.5228785516735357 1110 34-35 -0.06447559243091305 1110 36-37 0.31905236767558875 1110 38-39 -0.016198735290011257 1110 40-41 -0.015112372252289674 1110 42-43 0.10665431083826604 1110 44-45 0.4573716586993619 1110 46-47 -0.4527448216089667 1110 48-49 0.3872405060699329 1110 50-51 0.20191123649229326 1110 52-53 -0.7930978278587695 1110 54-55 -0.19501120659970894 1110 56-57 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0.4846007832560133 2116 44-45 2.1156831663110864 2116 46-47 0.3024115051220839 2116 48-49 0.4808319285613365 2116 50-51 0.7303528799691286 2116 52-53 0.7097054132608882 2116 54-55 0.22205787180919145 2116 56-57 -0.07442013319946739 2116 58-59 -0.01283205941713561 2116 60-61 -1.0283554801705002 2116 62-63 0.4442237396197726 2116 64-65 1.2916105163242868 2116 66-67 0.251864206339544 2116 68-69 -0.06349107988738112 2116 70-71 -2.527786103818727 2116 72-73 0.21452216463719154 2116 74-75 -1.2299568334057547 2116 76 -0.5337128892380214 2117 1 -0.15096037383248984 2117 2 1.1652688534310691 2117 3 1.159374670745649 2117 4 0.32585676272683983 2117 5 -1.5487361337266918 2117 6 -1.2526001111470038 2117 7 -1.3949217982778066 2117 8 -2.2064118732526694 2117 9 0.9901797422378991 2117 10-11 -0.11584959989070143 2117 12-13 1.1302506739087228 2117 14-15 -0.6296362496764978 2117 16-17 1.3739719028846942 2117 18-19 0.7584243545962579 2117 20-21 0.2062513566460673 2117 22-23 0.9006392463479322 2117 24-25 1.2196268672518542 2117 26-27 1.0430888336153323 2117 28-29 0.8461592503718833 2117 30-31 0.7743334111962987 2117 32-33 1.436608576904817 2117 34-35 0.3278288650964214 2117 36-37 -0.6464017954867316 2117 38-39 1.0846633336755076 2117 40-41 0.13326862017832752 2117 42-43 -0.44761398184466117 2117 44-45 0.32963826929650253 2117 46-47 0.4253460110234961 2117 48-49 -0.46082509527573734 2117 50-51 1.3481265434729508 2117 52-53 1.8779072183985939 2117 54-55 0.7977347984465482 2117 56-57 -0.34389479184330085 2117 58-59 0.2534951796478957 2117 60-61 1.6655579657910842 2117 62-63 0.48400619738417916 2117 64-65 0.5799465496499074 2117 66-67 0.4963215078901122 2117 68-69 0.4526163026629568 2117 70-71 1.742938265540218 2117 72-73 1.2853159623692 2117 74-75 0.020540736601187604 2117 76 0.31294297745950317 2118 1 1.8810145477976015 2118 2 2.4932581055582546 2118 3 2.3956039586990734 2118 4 2.070953045754159 2118 5 1.568908290814285 2118 6 1.7907496425475742 2118 7 2.157026253670246 2118 8 -2.434714605003677 2118 9 0.507331555941434 2118 10-11 1.3323366965714563 2118 12-13 2.082467422677194 2118 14-15 1.6258944265438338 2118 16-17 1.3473709176630173 2118 18-19 0.5189035305926737 2118 20-21 -2.2302690195294836 2118 22-23 0.9560131827563474 2118 24-25 2.0642529308434376 2118 26-27 2.139259903924337 2118 28-29 2.0228050183969657 2118 30-31 1.8664516377972795 2118 32-33 1.7846381335550632 2118 34-35 2.451362228284953 2118 36-37 1.8072927857940613 2118 38-39 1.803606459515187 2118 40-41 0.9782753375988378 2118 42-43 1.6296587454280669 2118 44-45 1.2413041895831114 2118 46-47 1.3914902116504564 2118 48-49 1.1824574842137388 2118 50-51 1.6477458896440211 2118 52-53 0.36238997701928 2118 54-55 1.6118861643220512 2118 56-57 2.4635167621199834 2118 58-59 2.034104225774186 2118 60-61 -0.03661400017398719 2118 62-63 2.282953801504192 2118 64-65 0.8605332043744944 2118 66-67 -0.49445323460877333 2118 68-69 2.225992926039577 2118 70-71 0.9701438186078413 2118 72-73 1.0589612825662407 2118 74-75 2.338856903193218 2118 76 1.3204664884312898 2119 1 -0.11964902220903184 2119 2 0.9486567215408108 2119 3 1.2436938250371163 2119 4 0.5691993250223355 2119 5 0.7943798994975708 2119 6 -0.2342764262741106 2119 7 -1.8109801924383895 2119 8 0.17823448969724254 2119 9 -0.023714040797592872 2119 10-11 0.07501372756965452 2119 12-13 0.4703714894133775 2119 14-15 0.22817900328096385 2119 16-17 -0.12134649630987226 2119 18-19 -0.1717543516710478 2119 20-21 -0.8060818927876134 2119 22-23 0.28042781921478266 2119 24-25 0.5573565425601856 2119 26-27 -0.06111453915083942 2119 28-29 0.4851474205203843 2119 30-31 0.0942931393614117 2119 32-33 0.22389777901909014 2119 34-35 0.6022059101505377 2119 36-37 -0.8272223341225242 2119 38-39 -0.5298848666768734 2119 40-41 -1.065472884981503 2119 42-43 -2.4717015730855323 2119 44-45 -0.697671068735211 2119 46-47 -0.6649132389135772 2119 48-49 -1.0565462128191534 2119 50-51 0.3086853151819824 2119 52-53 -1.7708217018128387 2119 54-55 -1.9062672151451991 2119 56-57 0.35097054920879955 2119 58-59 0.09922888213971603 2119 60-61 -0.4929368743800744 2119 62-63 0.1727300836162442 2119 64-65 0.3786628849129343 2119 66-67 0.3601775360803927 2119 68-69 -0.6327851571910621 2119 70-71 -1.0951941281169653 2119 72-73 -1.4731033681115484 2119 74-75 0.40848435603234634 2119 76 0.22756668750228926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 12.0 13 178.0 14 600.0 15 1229.0 16 1675.0 17 2045.0 18 2046.0 19 1897.0 20 1685.0 21 1348.0 22 981.0 23 801.0 24 737.0 25 707.0 26 780.0 27 887.0 28 980.0 29 1218.0 30 1423.0 31 1776.0 32 2162.0 33 2940.0 34 4111.0 35 5947.0 36 9979.0 37 11310.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.53926568509514 21.522847799935615 10.069297368733162 35.86858914623608 2 16.946700977176786 28.40203844795398 29.940965739946517 24.710294834922717 3 19.171838471500415 25.616832332610123 28.302807070656105 26.908522125233365 4 11.426745379013404 26.444321106009383 32.33092823384967 29.798005281127537 5 11.118959920614898 35.23218460399953 35.6223825621878 18.02647291319777 6 21.57357417965925 35.62911011319105 17.629547404006257 25.16776830314345 7 21.526481322636528 31.076239971744286 19.48130581764973 27.91597288796946 8 18.815278268328374 38.340313167499204 18.78836806431539 24.05604049985704 9 20.667036681971844 22.82153489076139 19.41066653211565 37.100761895151116 10-11 20.513984896647997 27.011958221908273 26.211379652521995 26.262677228921742 12-13 20.583783238306673 24.748137309315975 25.382208991371918 29.28587046100543 14-15 19.560354541937873 26.604941386211884 23.13268412466152 30.702019947188724 16-17 20.922683620095196 28.60554686580218 24.100610525253543 26.371158988849086 18-19 19.939620229745866 27.064937686058833 24.910439477269286 28.08500260692601 20-21 19.090266915586053 27.53334342465984 24.894461543636577 28.481928116117533 22-23 21.8443581075399 27.396269572968702 24.274685907462537 26.484686412028864 24-25 20.265065509527894 29.49694737373228 24.128361673141935 26.109625443597896 26-27 18.649612324873438 30.09233563751955 25.67653934776393 25.581512689843077 28-29 20.550986427165853 27.845333602435375 27.481204904384683 24.122475066014093 30-31 20.36093311132415 27.480363960509273 27.614914980574195 24.543787947592378 32-33 18.929646635383556 30.00067275510033 26.218948147400646 24.85073246211548 34-35 19.636880434599796 29.102544696166976 26.03898615806381 25.221588711169417 36-37 20.215449820878955 27.86719814319592 26.575508350572687 25.341843685352437 38-39 19.6814504599963 26.996821232150964 28.168256050591182 25.15347225726155 40-41 18.515061304808516 26.805086028558456 27.85962964831727 26.820223018315758 42-43 17.53367980221 26.1398994231125 28.77289469700792 27.553526077669577 44-45 17.56143095009839 26.6663302891165 29.179070588828903 26.593168171956204 46-47 17.872580183998522 27.408883731099788 28.719074288981954 25.999461795919743 48-49 17.31839817010613 27.861311536068083 28.26832837176447 26.551961922061324 50-51 17.051818961602503 27.715828245622887 27.191079267369695 28.041273525404915 52-53 16.49763694771011 27.619119699951227 27.965588576618394 27.91765477572027 54-55 16.397564626536827 27.78899036278319 27.482886792135492 28.330558218544493 56-57 17.290647022217737 27.816741510671577 26.576349294448086 28.316262172662597 58-59 17.395765006643458 27.202852481625378 26.51327850379266 28.88810400793851 60-61 16.488386565080646 26.655398018736232 27.24489967539566 29.611315740787457 62-63 17.365491027128847 26.92450005886607 26.672216896244343 29.037792017760733 64-65 17.954151739912877 27.46354508300116 25.129084884874786 29.453218292211176 66-67 17.446221639167803 28.687118421716534 24.436988075415847 29.42967186369982 68-69 18.675681585011016 28.285147249272587 24.07201843348975 28.96715273222665 70-71 19.170997527625005 28.202734749482822 24.487444707940192 28.13882301495198 72-73 20.342432346065223 28.459222631481573 23.568293052121703 27.630051970331497 74-75 19.582219082698423 28.59629648317271 24.023243688716214 27.79824074541265 76 19.958961938880197 27.700691255865586 24.262912693206857 28.07743411204736 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.5 10 2.0 11 3.0 12 3.0 13 3.5 14 3.5 15 5.0 16 8.5 17 10.0 18 13.5 19 19.0 20 29.5 21 38.0 22 50.0 23 65.0 24 97.5 25 127.0 26 142.5 27 198.5 28 292.5 29 346.0 30 396.5 31 516.5 32 730.0 33 874.0 34 991.0 35 1248.5 36 1541.5 37 1694.0 38 1948.0 39 2452.0 40 2957.0 41 3384.5 42 3557.0 43 3681.5 44 3974.5 45 4168.5 46 4194.0 47 4220.0 48 4157.0 49 3925.5 50 3783.0 51 3561.0 52 3064.5 53 2580.5 54 2371.0 55 2191.5 56 1763.5 57 1376.5 58 1238.0 59 1046.0 60 727.5 61 537.5 62 474.0 63 399.0 64 261.5 65 177.0 66 138.0 67 121.0 68 102.0 69 72.0 70 48.0 71 35.0 72 35.5 73 26.0 74 16.0 75 16.0 76 16.5 77 12.0 78 7.5 79 8.0 80 9.5 81 9.0 82 6.0 83 5.0 84 4.5 85 3.5 86 3.0 87 3.0 88 3.0 89 3.5 90 5.0 91 5.0 92 4.0 93 3.5 94 2.0 95 0.5 96 0.0 97 0.0 98 2.0 99 6.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7333030593538187 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 59457.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.1392939435222 #Duplication Level Percentage of deduplicated Percentage of total 1 83.32161248610512 61.774055199555974 2 8.965313854041423 13.293640782414181 3 3.7930173997867564 8.436348958070539 4 1.6129398153399426 4.7832887633079375 5 0.8711236133481546 3.229224481558101 6 0.5126925432726118 2.2806397901004085 7 0.3312084571584129 1.7188892813293641 8 0.1950953925727638 1.1571387725583195 9 0.12703886027993921 0.8476714264090015 >10 0.2699575780948708 2.479102544696167 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005045663252434532 0.0 2 0.0 0.0 0.0 0.006727551003246043 0.0 3 0.0 0.0 0.0 0.006727551003246043 0.0 4 0.0 0.0 0.0 0.006727551003246043 0.0 5 0.0 0.0 0.0 0.006727551003246043 0.0 6 0.0 0.0 0.0 0.006727551003246043 0.0 7 0.0 0.0 0.0 0.006727551003246043 0.0 8 0.0 0.0 0.0 0.006727551003246043 0.0 9 0.0 0.0 0.0 0.011773214255680576 0.0 10 0.0 0.0 0.0 0.011773214255680576 0.0 11 0.0 0.0 0.0 0.011773214255680576 0.0 12 0.0 0.0 0.0 0.011773214255680576 0.0 13 0.0 0.0 0.0 0.013455102006492086 0.0 14 0.0 0.0 0.0 0.015136989757303598 0.0 15 0.0 0.0 0.0 0.015136989757303598 0.0 16 0.0 0.0 0.0 0.015136989757303598 0.0 17 0.0 0.0 0.0 0.01681887750811511 0.0 18 0.0 0.0 0.0 0.026910204012984173 0.0 19 0.0 0.0 0.0 0.03363775501623022 0.0 20 0.0 0.0 0.0 0.045410969271910795 0.0 21 0.0 0.0 0.0 0.047092857022722305 0.0 22 0.0 0.0 0.0 0.050456632524345325 0.0 23 0.0 0.0 0.0 0.052138520275156836 0.0 24 0.0 0.0 0.0 0.053820408025968346 0.0 25 0.0 0.0 0.0 0.06054795902921439 0.0 26 0.0 0.0 0.0 0.06559362228164892 0.0 27 0.0 0.0 0.0 0.09586760179625611 0.0 28 0.0 0.0 0.0 0.11605025480599425 0.0 29 0.0 0.0 0.0 0.1379147955665439 0.0 30 0.0 0.0 0.0 0.1715525505827741 0.0 31 0.0 0.0 0.0 0.2169635198546849 0.0 32 0.0 0.0 0.0 0.2741477033822763 0.0 33 0.0 0.0 0.0 0.33133188690986765 0.0 34 0.0 0.0 0.0 0.39187984593908204 0.0 35 0.0 0.0 0.0 0.4776561212304691 0.0 36 0.0 0.0 0.0 0.6357535698067511 0.0 37 0.0 0.0 0.0 0.8880367324284777 0.0 38 0.0 0.0 0.0 1.2530063743545756 0.0 39 0.0 0.0 0.0 1.6062028020249928 0.0 40 0.0 0.0 0.0 2.0350841784819282 0.0 41 0.0 0.0 0.0 2.7465226970751973 0.0 42 0.0 0.0 0.0 3.377230603629514 0.0 43 0.0 0.0 0.0 4.07353213246548 0.0 44 0.0 0.0 0.0 4.791698202061994 0.0 45 0.0 0.0 0.0 5.382040802596834 0.0 46 0.0 0.0 0.0 6.041340800914947 0.0 47 0.0 0.0 0.0 6.741006105252535 0.0 48 0.0 0.0 0.0 7.3347124812889986 0.0 49 0.0 0.0 0.0 7.805641051516222 0.0 50 0.0 0.0 0.0 8.365709672536456 0.0 51 0.0 0.0 0.0 8.919050742553441 0.0 52 0.0 0.0 0.0 9.586760179625612 0.0 53 0.0 0.0 0.0 10.380611198008644 0.0 54 0.0 0.0 0.0 11.08700405334948 0.0 55 0.0 0.0 0.0 11.716030072152984 0.0 56 0.0 0.0 0.0 12.42242292749382 0.0 57 0.0 0.0 0.0 13.012765528028659 0.0 58 0.0 0.0 0.0 13.574516036799704 0.0 59 0.0 0.0 0.0 14.072354811039911 0.0 60 0.0 0.0 0.0 14.60551322804716 0.0 61 0.0 0.0 0.0 15.130262206300351 0.0 62 0.0 0.0 0.0 15.623055317288124 0.0 63 0.0 0.0 0.0 16.13939485678726 0.0 64 0.0 0.0 0.0 16.692735926804247 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTATG 15 0.0022152518 69.99411 2 ACAGCAT 15 0.0022152518 69.99411 55 GAATTCG 15 0.0022152518 69.99411 47 GATTCCG 15 0.0022152518 69.99411 68 TTCGAAC 15 0.0022152518 69.99411 50 CCCGATT 15 0.0022152518 69.99411 65 GATGTTT 20 0.006922119 52.495586 52 CTTCCGA 20 0.006922119 52.495586 18 ATTCCGC 20 0.006922119 52.495586 69 AATTCGA 20 0.006922119 52.495586 48 GACGCTT 20 0.006922119 52.495586 14 GCTCCAG 20 0.006922119 52.495586 12 TACAAGA 20 0.006922119 52.495586 27 TCTATGT 20 0.006922119 52.495586 3 TCGCCGT 30 5.8367033E-4 46.66274 49 GACCCTA 35 0.0012467052 39.996635 18 TGGTCGC 35 0.0012467052 39.996635 46 CGACGAG 40 0.00240208 34.997055 19 TTATGAA 40 0.00240208 34.997055 43 GGTCGCC 55 2.961142E-4 31.815504 47 >>END_MODULE