FastQCFastQC Report
Wed 25 May 2016
SRR1780500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59457
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT28884.857291824343643No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT27614.643692079990582TruSeq Adapter, Index 9 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC9691.6297492305363541RNA PCR Primer, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC4560.766940814370049TruSeq Adapter, Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG2910.48942933548614964No Hit
TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT2820.47429234572884604RNA PCR Primer, Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT2300.3868341826866475No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1520.25564693812334965No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT1200.2018265300973813No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT1190.20014464234656978No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG1100.18500765258926619No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT1040.1749163260843971No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC970.16314311182871655No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT930.1564155608254705No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC920.15473367307465902No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG910.15305178532384747No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC830.1395966833173554TruSeq Adapter, Index 9 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC730.1227778058092403TruSeq Adapter, Index 9 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTCTT650.1093227038027482No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAC150.002214512670.0000156
CAGGGAT150.002214512670.0000118
CTTGACA150.002214512670.0000157
ATCTTGC150.002214512670.0000145
GGTATAC150.002214512670.000013
CTATTGT150.002214512670.000014
TTTACAC1500.060.6666682
CTTTACA1550.058.709681
TTACACA1550.058.709683
CTATACA800.056.8751
TCTGCCA200.00691981752.511
CATCTTG200.00691981752.544
ACTTCAT200.00691981752.540
CTAAAGC200.00691981752.515
GAGCCCG200.00691981752.531
TTTGCAA200.00691981752.556
CTTTATA352.0634527E-550.02
GATAGTG305.8342825E-446.6666685
TTTATAC503.425328E-642.03
TTAATAC602.5447298E-740.8333362
Produced by FastQC (version 0.11.5)