FastQCFastQC Report
Wed 25 May 2016
SRR1780499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595899
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA301.293966E-436.697324
CGACTGG250.002335459735.25016412
CCCGTAA250.002357726535.18205625
GTGTCGC300.005724011529.35290136
CGGAGTT407.059279E-427.47905528
TACATGC905.2204996E-1026.8458632
TGGCGAC508.225755E-526.45098116
CCCGTAC508.24935E-526.4398513
GTACATG1051.1277734E-1025.12161
CGAGAAC1550.024.11673225
TATACCG651.8327848E-523.6563846
ACTAATT703.168445E-522.03877318
ATTTCGC500.002590346821.98324628
GACTATA500.002591610421.98141
TAAGACT1251.0113581E-921.0897469
GCGAACT1158.825737E-921.0274528
TCTCGGG1800.020.74975643
CCCGTGT856.1586506E-620.684933
AGAACGG1601.8189894E-1220.60929127
TCGGTCA755.49526E-520.51941929
Produced by FastQC (version 0.11.5)