FastQCFastQC Report
Wed 25 May 2016
SRR1780499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595899
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT373446.266833809085097No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT301555.060421313007741No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC94761.5902023665084184No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC52580.8823642932778877No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG46960.7880530089830659No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT29020.4869952794013751No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC22130.37137165862000104No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC13720.23024035952401328No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG13490.22638064504219677No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG11400.19130758735960288No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC10600.1778824935098062No Hit
CTTAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC9180.15405295192641708No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC7050.11830863955133335No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG6020.10102383121972011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT30300.042.61696644
GCCGTCT33350.038.5875443
CTATACA6350.037.4645541
CTTATAA2050.036.5338631
TTTACAC12150.035.4987142
CTTTACA12800.033.557931
TTACACA13450.032.2312243
ACCGAAT353.2174663E-431.42599334
TTATACA57200.029.930672
CTTATAC58550.029.4205251
CCGCTTT300.00574265229.3333912
CCGACTC300.005745007729.3309299
TAACCGG407.0312887E-427.49774622
CGAATTG407.0312887E-427.49774636
TTGCGAG407.0312887E-427.4977469
CCGAATT407.0312887E-427.49774635
TATACAC65200.026.46073
CTTTATA2250.026.4066982
CGCAAAG508.334352E-526.40005112
CCAACGT508.339112E-526.39783722
Produced by FastQC (version 0.11.5)