FastQCFastQC Report
Wed 25 May 2016
SRR1780498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54174
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACTAG150.002173703970.320041
CACTAGA150.002214020869.9953842
CCCTAAG200.006792676652.740031
AGACGAG200.006918300452.49653655
AGGCAGG200.006918300452.49653614
GCTAGTC200.006918300452.4965368
TACTCTG200.006918300452.49653643
GCAGGCG200.006918300452.49653616
TTTTACG200.006918300452.49653651
GGCTAAG200.006918300452.49653655
ACTTTTA350.001245607439.9973651
GATTATT450.004274029831.10905834
CTGATGA450.004274029831.10905829
CGACGAA2052.1043837E-413.65763670
CCGACGA2650.001821730910.56534169
TGCCGAC3500.00176611858.99940770
GCCGACG3150.00760481378.88830368
CTGCCGA3950.0052771557.974157369