##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780498_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54174 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.73173478052202 33.0 23.0 34.0 12.0 34.0 2 29.154188356037952 34.0 27.0 34.0 12.0 34.0 3 29.37482925388563 34.0 27.0 34.0 11.0 34.0 4 29.979750433787427 34.0 31.0 34.0 11.0 34.0 5 29.789253147266216 34.0 31.0 34.0 11.0 34.0 6 32.08840033964633 37.0 31.0 38.0 11.0 38.0 7 31.200188282201793 36.0 27.0 38.0 11.0 38.0 8 30.08171816738657 35.0 25.0 38.0 11.0 38.0 9 30.920386163104073 36.0 26.0 38.0 12.0 38.0 10-11 31.451997268062172 37.0 27.5 38.0 11.0 38.0 12-13 31.91492228744416 37.0 29.5 38.0 11.0 38.0 14-15 31.50333185661018 37.0 27.0 38.0 11.0 38.0 16-17 31.317597002252004 37.0 27.0 38.0 11.0 38.0 18-19 31.36070808875106 37.0 27.5 38.0 11.0 38.0 20-21 31.4468841141507 37.0 27.0 38.0 11.0 38.0 22-23 31.350610994203862 37.0 27.0 38.0 11.0 38.0 24-25 31.854145900247353 37.0 29.5 38.0 11.0 38.0 26-27 31.910879757817405 37.0 31.0 38.0 11.0 38.0 28-29 31.940349614206077 37.0 31.0 38.0 11.0 38.0 30-31 31.78239561413224 37.0 28.5 38.0 11.0 38.0 32-33 31.66358400708827 37.0 27.0 38.0 11.0 38.0 34-35 31.457341159966035 37.0 27.0 38.0 11.0 38.0 36-37 31.406264997969505 37.0 27.0 38.0 11.0 38.0 38-39 31.92398567578543 37.0 31.0 38.0 11.0 38.0 40-41 31.38483405323587 37.0 27.0 38.0 11.0 38.0 42-43 31.777448591575293 37.0 29.0 38.0 11.0 38.0 44-45 30.801768375973715 36.0 26.5 38.0 11.0 38.0 46-47 31.525473474360396 37.0 27.0 38.0 11.0 38.0 48-49 31.286973455901354 37.0 27.0 38.0 11.0 38.0 50-51 31.261795326171224 36.5 26.5 38.0 11.0 38.0 52-53 31.241407317163215 37.0 27.0 38.0 11.0 38.0 54-55 30.95131428360468 36.0 26.5 38.0 11.0 38.0 56-57 31.04853804408019 36.5 26.5 38.0 11.0 38.0 58-59 30.84354118211688 36.5 26.5 38.0 11.0 38.0 60-61 30.890417912651827 36.0 26.5 38.0 11.0 38.0 62-63 30.939694318307676 36.0 26.5 38.0 11.0 38.0 64-65 30.951618857754642 36.5 26.5 38.0 11.0 38.0 66-67 31.025205818289216 36.5 26.5 38.0 11.0 38.0 68-69 30.59473179015764 36.0 25.5 38.0 11.0 38.0 70-71 30.5105308819729 36.0 25.0 38.0 11.0 38.0 72-73 30.518163694761327 36.0 25.0 38.0 11.0 38.0 74-75 30.109148299922474 35.5 24.5 38.0 11.0 38.0 76 29.2629490161332 34.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.3457120472999051 1101 2 -0.36034460220253095 1101 3 -0.18085315514227673 1101 4 -2.18611034557523 1101 5 -0.6399798024471544 1101 6 -2.693688422412027 1101 7 -0.8670083581361077 1101 8 -0.23719418948519788 1101 9 0.181916678070845 1101 10-11 -1.856708542103494 1101 12-13 -0.9501926599071098 1101 14-15 -0.5741950550391124 1101 16-17 -2.1604693514062987 1101 18-19 -1.1587522462177908 1101 20-21 -0.8032641522589188 1101 22-23 -0.379194778717455 1101 24-25 -1.4823482024944923 1101 26-27 -1.1890183651976045 1101 28-29 -1.007950111556461 1101 30-31 -1.0986009823270706 1101 32-33 0.23378537695029422 1101 34-35 -0.911569895558511 1101 36-37 0.7374950356199363 1101 38-39 -0.9443136988025316 1101 40-41 0.5476582042464706 1101 42-43 0.23499829246235393 1101 44-45 -1.0317533795991878 1101 46-47 0.677714366936133 1101 48-49 -0.9717618005086237 1101 50-51 -2.042929014245164 1101 52-53 0.0836136856577312 1101 54-55 -0.29043628487443485 1101 56-57 -0.7892407517496345 1101 58-59 0.8490493767461018 1101 60-61 0.5503707356310983 1101 62-63 -1.151654743240016 1101 64-65 -0.5325148908326405 1101 66-67 0.01886635219782562 1101 68-69 0.13888742636568452 1101 70-71 -0.38481768692711427 1101 72-73 -1.1535841422650783 1101 74-75 -0.42757322612798276 1101 76 0.24290454932495464 1102 1 -0.28532304803189135 1102 2 -0.14895696234056288 1102 3 -0.3286624200173165 1102 4 -0.1381651400957793 1102 5 1.014574207800674 1102 6 0.4903542420238196 1102 7 2.107267496721157 1102 8 1.6317276969521117 1102 9 0.8063546159564474 1102 10-11 -0.12981844485367944 1102 12-13 -4.445199456937331 1102 14-15 -1.3655785121132524 1102 16-17 -2.3450348643206524 1102 18-19 -2.143040649438536 1102 20-21 -0.5983859759544146 1102 22-23 -1.5065868105066436 1102 24-25 0.13126677188593305 1102 26-27 -5.973238798116149 1102 28-29 -1.0847565530497185 1102 30-31 -0.4454063781233728 1102 32-33 -6.212674938850185 1102 34-35 -1.6190361718316453 1102 36-37 0.11985412795497297 1102 38-39 0.19110405713179546 1102 40-41 -0.7397254619670584 1102 42-43 -0.040751995104312044 1102 44-45 -6.261179291969782 1102 46-47 -0.5895396328547307 1102 48-49 -0.04511744204370771 1102 50-51 -5.3225222381288795 1102 52-53 -1.4982643860770821 1102 54-55 0.5115871387374042 1102 56-57 -1.3431202874601844 1102 58-59 0.8239787922015012 1102 60-61 0.26445104515739715 1102 62-63 -3.9937022178818644 1102 64-65 -0.6113604413920903 1102 66-67 0.4545821317649086 1102 68-69 -6.689539845410955 1102 70-71 -0.48923052808574496 1102 72-73 -0.26000995006597805 1102 74-75 -6.687585774491467 1102 76 -1.8812719643213924 1103 1 -1.4266757877579188 1103 2 -1.2257549075460403 1103 3 -0.7799466665926573 1103 4 -0.7048603455752307 1103 5 -0.23148743603494282 1103 6 -2.5044231552364558 1103 7 -0.8361400375254213 1103 8 -0.10885449482870868 1103 9 -0.1402207265093054 1103 10-11 0.3235634044613924 1103 12-13 -1.1536754843345918 1103 14-15 -1.187410703894077 1103 16-17 -1.1339903437727088 1103 18-19 -1.075617704233057 1103 20-21 -1.0851582362283878 1103 22-23 -0.8274772214655499 1103 24-25 -0.11295411852502468 1103 26-27 -1.5148055789380663 1103 28-29 -0.7076161420908136 1103 30-31 -0.27729850141103896 1103 32-33 -5.137803363507722 1103 34-35 -1.4392416512837016 1103 36-37 -1.348168132318996 1103 38-39 -0.23458087437504815 1103 40-41 -0.45475114689856255 1103 42-43 -0.439596173186505 1103 44-45 -4.5200405933396475 1103 46-47 -0.6558495643615778 1103 48-49 0.24965995521656836 1103 50-51 -0.5549280600466879 1103 52-53 -0.6352078792277638 1103 54-55 -0.0757416283858845 1103 56-57 0.38945676733433743 1103 58-59 -0.8178876461546594 1103 60-61 0.35075241502041266 1103 62-63 -0.18612516308734328 1103 64-65 0.038596750388734336 1103 66-67 -0.8172500600159154 1103 68-69 -2.893221352260273 1103 70-71 -0.05006100753780274 1103 72-73 0.023037995139503664 1103 74-75 -1.7135703635325648 1103 76 -0.4928301834994748 1104 1 -2.676675787757919 1104 2 -0.13825490754604175 1104 3 0.48255333340734197 1104 4 0.59513965442477 1104 5 0.23726256396505718 1104 6 -0.37942315523645576 1104 7 1.5138599624745765 1104 8 2.04114550517129 1104 9 0.11602927349069247 1104 10-11 0.4641884044613924 1104 12-13 -0.6349254843345946 1104 14-15 0.47508929610592077 1104 16-17 0.1316346562272912 1104 18-19 1.4587572957669401 1104 20-21 1.896091763771615 1104 22-23 0.9693977785344501 1104 24-25 0.6089208814749725 1104 26-27 1.1726944210619337 1104 28-29 0.7580088579091893 1104 30-31 0.39457649858896104 1104 32-33 0.16219663649228266 1104 34-35 0.7920083487163012 1104 36-37 0.551831867680999 1104 38-39 0.299794125624949 1104 40-41 1.2702488531014318 1104 42-43 0.8010288268135035 1104 44-45 -2.388790593339646 1104 46-47 0.7472754356384179 1104 48-49 1.4090349552165726 1104 50-51 2.3919469399533106 1104 52-53 0.7804171207722348 1104 54-55 0.6992583716141141 1104 56-57 1.4707067673343381 1104 58-59 1.250862353845342 1104 60-61 0.5882524150204098 1104 62-63 1.2388748369126539 1104 64-65 0.8667217503887343 1104 66-67 0.6389999399840853 1104 68-69 -3.4557213522602694 1104 70-71 -0.2750610075378006 1104 72-73 0.6355379951395008 1104 74-75 -0.5979453635325669 1104 76 0.6509198165005259 1105 1 -0.6359745682457252 1105 2 -0.07804149291189688 1105 3 -0.66302593488534 1105 4 -0.3263542480142547 1105 5 0.1980857346967646 1105 6 -0.01616096011450452 1105 7 1.2070002063770175 1105 8 0.9170601393176341 1105 9 1.273193907637033 1105 10-11 1.466322550802861 1105 12-13 -0.5111449965297155 1105 14-15 0.5447539302522628 1105 16-17 1.0878084367150933 1105 18-19 0.25860485674255074 1105 20-21 -0.08310030939911783 1105 22-23 1.4432544858515257 1105 24-25 0.5352928326944877 1105 26-27 0.5116425917936454 1105 28-29 -0.10776858111520227 1105 30-31 0.6831435717596932 1105 32-33 1.5776691974678911 1105 34-35 0.5302705438382489 1105 36-37 0.7753836969492909 1105 38-39 0.8483459548932473 1105 40-41 0.9073677555404593 1105 42-43 0.9741995585208194 1105 44-45 2.3091514798310833 1105 46-47 1.4061168990530497 1105 48-49 1.2937148332653443 1105 50-51 0.5253310862947735 1105 52-53 -0.06943044020337652 1105 54-55 0.07059983502875156 1105 56-57 -0.3636682326656633 1105 58-59 0.1895818660404629 1105 60-61 0.7507524150204112 1105 62-63 1.1717254466687557 1105 64-65 0.8563558967301965 1105 66-67 1.5021096960816465 1105 68-69 1.6499188916421659 1105 70-71 1.2241005778280503 1105 72-73 1.1568794585541369 1105 74-75 -0.4453539001179294 1105 76 -0.5246899395970352 1106 1 -1.660023689855823 1106 2 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-1.3244224101544155 1106 62-63 -0.38837603721321656 1106 64-65 0.7974909811579636 1106 66-67 -0.38608747260332876 1106 68-69 0.6482559204670011 1106 70-71 -0.8819884550902479 1106 72-73 -0.7482032636017593 1106 74-75 -1.4937932656304689 1106 76 0.39322750880822 1107 1 -0.5912591210912517 1107 2 0.8781141400730057 1107 3 0.6149938095978165 1107 4 0.8793658449009598 1107 5 0.7738697068222002 1107 6 0.8059935114302093 1107 7 1.593324248188864 1107 8 1.739359790885576 1107 9 -0.45212548841406885 1107 10-11 0.5546645949375808 1107 12-13 0.6126935632844592 1107 14-15 0.024791677058303208 1107 16-17 0.8555929895606198 1107 18-19 1.7257215814812277 1107 20-21 1.5468358113906646 1107 22-23 0.19514182615349895 1107 24-25 -0.04971007090597723 1107 26-27 1.3999265639190739 1107 28-29 -0.4531518563765289 1107 30-31 0.4192788795413378 1107 32-33 1.4852621126827614 1107 34-35 1.033972634430583 1107 36-37 0.9823378200619501 1107 38-39 0.6386334113392351 1107 40-41 1.5855762340538142 1107 42-43 1.445671683956359 1107 44-45 0.8488582161841656 1107 46-47 1.0960849594479463 1107 48-49 0.9428147171213261 1107 50-51 1.6255778923342596 1107 52-53 1.3444052160103297 1107 54-55 1.2248536097093563 1107 56-57 1.258355576858147 1107 58-59 1.5568147347977188 1107 60-61 0.8311095578775536 1107 62-63 -0.02615492499210248 1107 64-65 0.9446979408649234 1107 66-67 1.1187618447459897 1107 68-69 1.857671504882589 1107 70-71 0.5625878019860089 1107 72-73 1.1266094237109314 1107 74-75 0.46782844599124473 1107 76 -0.43598494540423616 1108 1 -1.7396331048310891 1108 2 -0.9987732002289675 1108 3 -0.6203430080560715 1108 4 -1.1678176626483996 1108 5 -0.6616703628642107 1108 6 -0.07713656987060347 1108 7 0.164317279547749 1108 8 0.831694285659097 1108 9 0.16953537105166916 1108 10-11 0.43278596543699877 1108 12-13 0.2540989059093093 1108 14-15 0.22158319854494835 1108 16-17 -1.0402403437727088 1108 18-19 -0.08590733837939979 1108 20-21 0.04189969060088217 1108 22-23 -0.047599172685060864 1108 24-25 -1.1750730209640494 1108 26-27 -0.5615281399136762 1108 28-29 0.6056460530311369 1108 30-31 0.12826552297920202 1108 32-33 0.33376675844349535 1108 34-35 -0.7380221390885779 1108 36-37 -0.5813236201238752 1108 38-39 -0.1790930694970001 1108 40-41 -0.8243395615327138 1108 42-43 -0.36726385611333257 1108 44-45 0.019517333489623923 1108 46-47 0.5737998258823218 1108 48-49 -0.9623827277102635 1108 50-51 -0.10881525516863988 1108 52-53 -0.392601171910691 1108 54-55 0.02791690819947945 1108 56-57 0.6119415234318986 1108 58-59 -1.1793205729839293 1108 60-61 0.46111826867894834 1108 62-63 -0.03864040698978144 1108 64-65 -1.13449776180639 1108 66-67 -0.6564268892842051 1108 68-69 0.5676018184714344 1108 70-71 0.42227130953537184 1108 72-73 -0.31568151705562286 1108 74-75 0.23757292915036032 1108 76 0.42652957259808844 1109 1 -2.3821682120003445 1109 2 -1.4725352105763463 1109 3 -0.6255906059865985 1109 4 -0.7558073152722002 1109 5 -0.22656319361070132 1109 6 -0.4235519431152426 1109 7 -2.511329431464816 1109 8 -0.5675666160408284 1109 9 -0.237190423479003 1109 10-11 -0.5877055349325495 1109 12-13 -0.6379557873648949 1109 14-15 -0.9709334311668059 1109 16-17 -1.656907010439376 1109 18-19 -1.0634017951421484 1109 20-21 -0.30608626653141613 1109 22-23 -0.9191438881322185 1109 24-25 -0.773465482161388 1109 26-27 -1.2069457304532136 1109 28-29 -1.3403812936059651 1109 30-31 -1.4044765317140744 1109 32-33 -0.8814586665380233 1109 34-35 -1.0066658937079467 1109 36-37 -1.1348158595917255 1109 38-39 -0.11109602589019829 1109 40-41 -0.3434822075046249 1109 42-43 -0.6610923853077146 1109 44-45 -1.228468623642673 1109 46-47 -1.469012443149456 1109 48-49 -1.3827264084197957 1109 50-51 0.011454515710884294 1109 52-53 -0.31939348528837286 1109 54-55 -0.21437799202224994 1109 56-57 -0.09245611145354005 1109 58-59 -0.7889103734273881 1109 60-61 -0.41136879710080265 1109 62-63 -1.40212895096613 1109 64-65 -1.324187340520357 1109 66-67 -0.7401667266825811 1109 68-69 -0.015380443169362934 1109 70-71 -0.8314057045074996 1109 72-73 -1.737946853345349 1109 74-75 -0.08563475747195781 1109 76 -1.8687771531964437 1110 1 0.2800488957863827 1110 2 -0.8087454138751546 1110 3 -0.9608802108964554 1110 4 -0.5088951557018113 1110 5 -0.236076043629879 1110 6 -0.31162252232506304 1110 7 -0.31106092360137083 1110 8 1.2447847456776202 1110 9 -1.4149042708131034 1110 10-11 -1.7109698233867086 1110 12-13 -0.8178368767396549 1110 14-15 -1.6227746279447075 1110 16-17 -1.0972023690891675 1110 18-19 0.11801362488087008 1110 20-21 -0.36945412230433305 1110 22-23 -0.7164012720984587 1110 24-25 -0.31805696662629046 1110 26-27 -1.859228047292497 1110 28-29 -0.346461078799674 1110 30-31 -1.0779709697654738 1110 32-33 0.8785336618087314 1110 34-35 -0.8363935500178812 1110 36-37 -0.7588089551038095 1110 38-39 -0.48806188703327535 1110 40-41 -0.058825513987173395 1110 42-43 -0.2034015529333395 1110 44-45 0.2663913686856709 1110 46-47 -0.5250346909438584 1110 48-49 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2116 44-45 1.1732629780889248 2116 46-47 0.1010452769082626 2116 48-49 -0.025439251132638674 2116 50-51 0.23172868598505758 2116 52-53 1.0279369620420766 2116 54-55 0.6405282128839573 2116 56-57 0.22363335463592549 2116 58-59 -0.4878281223451353 2116 60-61 -1.4873428230748296 2116 62-63 0.624638725801546 2116 64-65 0.5071979408649234 2116 66-67 0.3161824796666224 2116 68-69 0.339814362025443 2116 70-71 -2.5772931503949437 2116 72-73 -0.1997794651779614 2116 74-75 -1.4964572682944706 2116 76 -1.3149531993724892 2117 1 -0.3769734068055399 2117 2 -0.05839379643493103 2117 3 0.6487239683279782 2117 4 0.22658806712318125 2117 5 -2.0753366423841477 2117 6 -0.5055144250777239 2117 7 -1.134850354985737 2117 8 -1.8320687805429934 2117 9 -0.7656175519061321 2117 10-11 0.07946618223916957 2117 12-13 0.9103126109035031 2117 14-15 -0.222232132465507 2117 16-17 -0.25055780409017103 2117 18-19 -0.4439212756616264 2117 20-21 -0.7527673632125129 2117 22-23 -0.578667697656023 2117 24-25 0.38382167512576615 2117 26-27 0.6310773575698718 2117 28-29 0.3246259214012497 2117 30-31 -0.333697310934852 2117 32-33 0.9247462396668809 2117 34-35 -0.13567022271227103 2117 36-37 -2.574209798985663 2117 38-39 -0.14212055691473324 2117 40-41 -1.5543047183271348 2117 42-43 -1.9203997446150716 2117 44-45 0.7645328193587666 2117 46-47 -0.9108594849965002 2117 48-49 -1.1881376638310535 2117 50-51 0.3944270986834688 2117 52-53 0.5160322001373174 2117 54-55 -1.1928051204493784 2117 56-57 -0.6255729945704225 2117 58-59 -0.8687805032975184 2117 60-61 0.9491651134331072 2117 62-63 -0.19678984562702695 2117 64-65 -0.8221671385001521 2117 66-67 -1.675881012396868 2117 68-69 0.09378261599369608 2117 70-71 -0.27570584880764315 2117 72-73 1.220855455456963 2117 74-75 -0.0877271095643124 2117 76 -1.997492881912173 2118 1 0.8050371752050438 2118 2 0.6230876850465492 2118 3 0.2767662963703046 2118 4 0.08055632109143573 2118 5 -1.017135584183091 2118 6 0.801363881800583 2118 7 1.393721073585688 2118 8 -2.601910050384266 2118 9 -0.9698503561389344 2118 10-11 -0.759422706649719 2118 12-13 0.23174118233207253 2118 14-15 -0.5621792224125954 2118 16-17 0.202352248819885 2118 18-19 -0.4206408523812044 2118 20-21 -2.879486939932093 2118 22-23 -0.48951425850258445 2118 24-25 -0.6320513407472461 2118 26-27 -0.08876391227139635 2118 28-29 -0.14812540135007168 2118 30-31 -1.6667660940036342 2118 32-33 -1.0784283635077188 2118 34-35 1.043165756123706 2118 36-37 -0.8297653545412196 2118 38-39 -0.304025318819491 2118 40-41 -1.4458391098615273 2118 42-43 -0.18971191392724407 2118 44-45 0.7018344066603568 2118 46-47 -0.5939282680652802 2118 48-49 -1.8746456003389902 2118 50-51 -0.6754141711578008 2118 52-53 -1.40962917552406 2118 54-55 0.0714805938363412 2118 56-57 0.4752206562232253 2118 58-59 0.3976216131045973 2118 60-61 -2.0844327701647742 2118 62-63 -0.20684275567993637 2118 64-65 -0.3073523236853397 2118 66-67 -2.0957222822381354 2118 68-69 1.6903434625545444 2118 70-71 -0.5825841556859466 2118 72-73 -1.5487212641197594 2118 74-75 -0.43243610427330736 2118 76 -0.4027838872031779 2119 1 1.460561607200063 2119 2 1.645621142874127 2119 3 1.7238873670207866 2119 4 1.6374190661894765 2119 5 2.6268108832927908 2119 6 2.9912176010660687 2119 7 1.0019376935670152 2119 8 3.3149900429864196 2119 9 1.118602802902462 2119 10-11 1.7029489086630711 2119 12-13 2.5104526669259144 2119 14-15 1.4815493801395405 2119 16-17 1.5753058747146866 2119 18-19 0.9846501529097971 2119 20-21 1.107410041082538 2119 22-23 2.718951349963021 2119 24-25 1.3296666797943004 2119 26-27 2.20507362274261 2119 28-29 3.053150664631872 2119 30-31 1.776246666656185 2119 32-33 1.678714493635141 2119 34-35 2.5197919621616762 2119 36-37 1.764725775244024 2119 38-39 1.968990554196381 2119 40-41 1.823846542177062 2119 42-43 0.6342221041244223 2119 44-45 0.19847306212254168 2119 46-47 1.4089350995039673 2119 48-49 1.1136830644602647 2119 50-51 2.5844364357516305 2119 52-53 0.10683518799912406 2119 54-55 0.5828718169922666 2119 56-57 2.718264540443581 2119 58-59 2.112311933677269 2119 60-61 2.4209204822473005 2119 62-63 1.9689431142235811 2119 64-65 1.7389906579517564 2119 66-67 2.1945671668748403 2119 68-69 1.4469572191682971 2119 70-71 0.9311102109495941 2119 72-73 -0.3003863746084008 2119 74-75 1.5133466532741586 2119 76 2.154701329105567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 15.0 13 157.0 14 516.0 15 788.0 16 1133.0 17 1310.0 18 1390.0 19 1363.0 20 1124.0 21 1007.0 22 823.0 23 767.0 24 853.0 25 808.0 26 947.0 27 1075.0 28 1284.0 29 1568.0 30 1774.0 31 2126.0 32 2701.0 33 3425.0 34 4453.0 35 6250.0 36 9173.0 37 7342.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.48141263940521 20.654275092936803 13.866171003717472 27.998141263940518 2 19.789936131723703 21.940414220843948 29.04714438660612 29.222505260826225 3 19.640417912651824 23.66079669214014 28.336471370029905 28.362314025178133 4 12.679514158083213 23.247314209768525 33.17643149850482 30.896740133643448 5 11.909772215453907 32.95861483368405 32.03197105622623 23.099641894635802 6 26.547790452984827 35.295529220659354 17.9754125595304 20.181267766825414 7 23.88415106877838 30.337431240078267 19.247240373610957 26.531177317532396 8 21.50847269908074 32.214715546202974 17.604385867759444 28.672425886956844 9 22.10100786355078 20.221877653486914 18.77838077306457 38.89873370989774 10-11 21.761361538745525 25.44763170524606 26.233063831358216 26.557942924650202 12-13 21.758592682836785 23.859231365599733 25.523313766751578 28.8588621848119 14-15 20.830103001439806 25.639605714918595 22.907667884963267 30.622623398678332 16-17 22.029017609923578 27.764241148890612 23.86015431756931 26.346586923616496 18-19 20.122198840772327 26.89943515339462 25.09506405286669 27.883301952966367 20-21 19.92653302322147 27.095100970945474 25.168900210433048 27.80946579540001 22-23 21.952412596448482 26.917894192786207 25.007383615756634 26.122309595008673 24-25 20.68796839812456 28.973308229039763 24.40285007568206 25.935873297153616 26-27 19.329383098903534 29.04899029054528 26.08262266031676 25.53900395023443 28-29 21.111603352161552 27.449514527264 27.15416989699856 24.284712223575884 30-31 20.843024329013918 27.204009303355853 27.07664193155388 24.876324436076345 32-33 19.026654852881457 29.343411968841142 26.509026470262487 25.120906708014918 34-35 20.33816960165393 28.53121423561118 26.28382618968509 24.846789973049802 36-37 20.588289585409974 27.526119540739103 26.644700409790673 25.24089046406025 38-39 20.325248274079815 27.26400118137852 27.313840587735815 25.096909956805845 40-41 19.825008306567725 26.63177908221656 27.28338317273969 26.259829438476025 42-43 18.90390224092738 26.019861926385353 28.26817292428102 26.808062908406242 44-45 18.57810019566582 26.46841658360099 28.56905526636394 26.384427954369254 46-47 19.158636984531324 26.389965666186733 28.356776313360655 26.09462103592129 48-49 17.972643703621664 27.458744046959797 28.06604644294311 26.50256580647543 50-51 18.108317643149853 27.45505223908148 27.23908147819987 27.197548639568797 52-53 17.872964890907078 26.924354856573263 27.80023627570421 27.402443976815444 54-55 17.492708679440323 27.340606194853617 27.241850334108612 27.924834791597448 56-57 17.940340384686383 27.131096097759073 27.120020674124117 27.808542843430427 58-59 18.284601469339535 26.39365747406505 27.22985195850408 28.091889098091332 60-61 17.748366375013845 26.445342784361504 26.956658175508547 28.84963266511611 62-63 18.08985860375826 26.519178941927862 26.8865138258205 28.50444862849337 64-65 18.662088824897552 27.041569756709862 25.5989958282571 28.697345590135487 66-67 18.155388193598405 28.3770812566914 24.689888138221285 28.77764241148891 68-69 18.14892752981135 27.986672573559275 24.805257134418728 29.059142762210655 70-71 18.47749843098165 28.673348838926422 24.42869273083029 28.420459999261638 72-73 18.974046590615423 28.365082881086867 23.969062649979694 28.69180787831801 74-75 19.034961420607672 28.81086868239377 24.07427917451176 28.0798907224868 76 19.63672610477351 27.54088677225237 24.90493594713331 27.917451175840807 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.5 11 1.0 12 0.0 13 2.0 14 4.0 15 3.0 16 4.5 17 7.0 18 9.5 19 13.0 20 18.5 21 23.0 22 29.5 23 44.0 24 61.5 25 71.0 26 79.5 27 143.5 28 222.0 29 245.0 30 294.5 31 403.5 32 537.0 33 611.0 34 721.0 35 995.5 36 1311.0 37 1462.0 38 1666.0 39 2131.0 40 2635.0 41 3004.0 42 3130.0 43 3255.5 44 3530.5 45 3745.5 46 3811.0 47 3811.0 48 3777.5 49 3638.5 50 3533.0 51 3257.0 52 2823.5 53 2432.0 54 2198.0 55 2034.5 56 1726.5 57 1397.5 58 1213.0 59 1050.0 60 798.0 61 631.5 62 554.0 63 502.5 64 398.0 65 297.0 66 206.0 67 163.0 68 152.5 69 119.5 70 75.0 71 53.0 72 44.0 73 33.0 74 25.0 75 19.0 76 14.0 77 7.0 78 7.5 79 10.0 80 9.5 81 5.5 82 2.0 83 2.0 84 2.0 85 2.0 86 3.0 87 4.0 88 3.0 89 2.5 90 3.5 91 3.5 92 3.0 93 2.5 94 1.5 95 0.5 96 0.0 97 0.5 98 2.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6903680732454683 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 54174.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.59947576328128 #Duplication Level Percentage of deduplicated Percentage of total 1 84.90734269947225 65.03857939232842 2 8.451213340723426 12.947170229261271 3 3.2725257247511865 7.520212648133791 4 1.3567245824999397 4.156975670986082 5 0.7783695206882425 2.9811348617417948 6 0.4819625515097477 2.215084726990807 7 0.24580090126997134 1.3179754125595304 8 0.2000144588765453 1.2256802156015802 9 0.10844157408969324 0.7475910953593975 >10 0.19760464611899656 1.849595747037324 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007383615756636024 0.0 2 0.0 0.0 0.0 0.007383615756636024 0.0 3 0.0 0.0 0.0 0.007383615756636024 0.0 4 0.0 0.0 0.0 0.009229519695795031 0.0 5 0.0 0.0 0.0 0.009229519695795031 0.0 6 0.0 0.0 0.0 0.011075423634954036 0.0 7 0.0 0.0 0.0 0.012921327574113043 0.0 8 0.0 0.0 0.0 0.014767231513272049 0.0 9 0.0 0.0 0.0 0.014767231513272049 0.0 10 0.0 0.0 0.0 0.014767231513272049 0.0 11 0.0 0.0 0.0 0.018459039391590062 0.0 12 0.0 0.0 0.0 0.02030494333074907 0.0 13 0.0 0.0 0.0 0.02030494333074907 0.0 14 0.0 0.0 0.0 0.02030494333074907 0.0 15 0.0 0.0 0.0 0.02399675120906708 0.0 16 0.0 0.0 0.0 0.02399675120906708 0.0 17 0.0 0.0 0.0 0.03138036696570311 0.0 18 0.0 0.0 0.0 0.03876398272233913 0.0 19 0.0 0.0 0.0 0.05353121423561118 0.0 20 0.0 0.0 0.0 0.059068926053088194 0.0 21 0.0 0.0 0.0 0.060914829992247205 0.0 22 0.0 0.0 0.0 0.06276073393140622 0.0 23 0.0 0.0 0.0 0.06460663787056521 0.0 24 0.0 0.0 0.0 0.07199025362720124 0.0 25 0.0 0.0 0.0 0.07383615756636025 0.0 26 0.0 0.0 0.0 0.07383615756636025 0.0 27 0.0 0.0 0.0 0.0904492930187913 0.0 28 0.0 0.0 0.0 0.10152471665374534 0.0 29 0.0 0.0 0.0 0.12921327574113042 0.0 30 0.0 0.0 0.0 0.17720677815926458 0.0 31 0.0 0.0 0.0 0.2122789530032857 0.0 32 0.0 0.0 0.0 0.25104293572562486 0.0 33 0.0 0.0 0.0 0.28426920663048694 0.0 34 0.0 0.0 0.0 0.35256765237937016 0.0 35 0.0 0.0 0.0 0.4282497138848894 0.0 36 0.0 0.0 0.0 0.5685384132609739 0.0 37 0.0 0.0 0.0 0.7900468859600547 0.0 38 0.0 0.0 0.0 1.0743160925905415 0.0 39 0.0 0.0 0.0 1.469339535570569 0.0 40 0.0 0.0 0.0 1.8938974415771404 0.0 41 0.0 0.0 0.0 2.427363679994093 0.0 42 0.0 0.0 0.0 3.1324989847528335 0.0 43 0.0 0.0 0.0 3.854247424964005 0.0 44 0.0 0.0 0.0 4.489238380034703 0.0 45 0.0 0.0 0.0 5.183298261158489 0.0 46 0.0 0.0 0.0 5.790600657141803 0.0 47 0.0 0.0 0.0 6.360984974341935 0.0 48 0.0 0.0 0.0 6.901834828515524 0.0 49 0.0 0.0 0.0 7.468527337837339 0.0 50 0.0 0.0 0.0 8.00383948019345 0.0 51 0.0 0.0 0.0 8.6185254919334 0.0 52 0.0 0.0 0.0 9.375346106988593 0.0 53 0.0 0.0 0.0 10.148779857496216 0.0 54 0.0 0.0 0.0 10.833610218924207 0.0 55 0.0 0.0 0.0 11.523978292169675 0.0 56 0.0 0.0 0.0 12.122051168457194 0.0 57 0.0 0.0 0.0 12.673976446265737 0.0 58 0.0 0.0 0.0 13.240668955587552 0.0 59 0.0 0.0 0.0 13.753830250673754 0.0 60 0.0 0.0 0.0 14.28360468121239 0.0 61 0.0 0.0 0.0 14.809687303872707 0.0 62 0.0 0.0 0.0 15.404068372281907 0.0 63 0.0 0.0 0.0 16.02613799977849 0.0 64 0.0 0.0 0.0 16.579909181526194 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACTAG 15 0.0021737039 70.32004 1 CACTAGA 15 0.0022140208 69.995384 2 CCCTAAG 20 0.0067926766 52.74003 1 AGACGAG 20 0.0069183004 52.496536 55 AGGCAGG 20 0.0069183004 52.496536 14 GCTAGTC 20 0.0069183004 52.496536 8 TACTCTG 20 0.0069183004 52.496536 43 GCAGGCG 20 0.0069183004 52.496536 16 TTTTACG 20 0.0069183004 52.496536 51 GGCTAAG 20 0.0069183004 52.496536 55 ACTTTTA 35 0.0012456074 39.99736 51 GATTATT 45 0.0042740298 31.109058 34 CTGATGA 45 0.0042740298 31.109058 29 CGACGAA 205 2.1043837E-4 13.657636 70 CCGACGA 265 0.0018217309 10.565341 69 TGCCGAC 350 0.0017661185 8.999407 70 GCCGACG 315 0.0076048137 8.888303 68 CTGCCGA 395 0.005277155 7.9741573 69 >>END_MODULE