FastQCFastQC Report
Wed 25 May 2016
SRR1780498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54174
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT18353.3872337283567764No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT16993.1361907926311514TruSeq Adapter, Index 8 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC5491.0134012625982944No Hit
CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC2700.49839406357293164No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG1570.28980691844796397No Hit
TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT1550.28611511056964595No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT1190.21966256875992174No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC960.17720677815926458No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC750.13844279543692545No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGT690.12736737180197144No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT670.1236755639236534No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG660.12182965998449441No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC610.11260014028869937No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT560.10337062059290435No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG150.00221344270.012
GCGGTTT150.00221344270.016
GTATGAT150.00221344270.03
TTTGCGG150.00221344270.013
TTTACAC1000.056.0000042
CTTTACA1050.053.3333321
AGATAAG200.006916495552.516
TGGCGGT200.006916495552.540
CGATGAT200.006916495552.523
CCCGGTA200.006916495552.565
TTCGATG200.006916495552.521
CCATTTA200.006916495552.557
GTCGAAC451.6560025E-646.66666432
TTTATAC305.8296404E-446.6666643
TCATCAC305.8296404E-446.66666470
TTACACA1300.045.769233
CTATACA802.910383E-1143.751
ATCGTAT404.5367782E-543.7539
AAACTCG459.086263E-538.88888533
AACTCGT459.086263E-538.88888534
Produced by FastQC (version 0.11.5)