FastQCFastQC Report
Wed 25 May 2016
SRR1780497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569523
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT250.00234093435.2330813
CGACGGA300.005738341729.3376722
AGTCGCC300.00577542429.299038
CTATAAC555.0037233E-627.9648044
CGTAGCG609.637402E-625.70209715
GTACAAG901.5093974E-824.4437621
AGCGGAA651.7895236E-523.7291918
GCTTCTA852.3487337E-723.2934671
CGACAAT952.6424459E-823.18782217
GCGGAAT602.8401468E-422.03424819
GGTAGGC1105.182301E-922.02067813
ACGTCGT500.00258974421.98392531
CGGAATC654.8676386E-420.3446820
CGTTACA654.896625E-420.3267814
GCCACGT654.9462373E-420.2964228
GCTATAC654.952102E-420.29285231
TACATAC654.969731E-420.2821643
TACGATA550.004465531620.01175537
GGTCATA550.00448146919.999441
CTTAGAC550.00448146919.999441
Produced by FastQC (version 0.11.5)