FastQCFastQC Report
Wed 25 May 2016
SRR1780497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569523
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT273544.802966693180082No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT215053.775966905638578RNA PCR Primer, Index 17 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC67801.190469919564267No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC34390.6038386509412262No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG29960.5260542594416731No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT19250.33800215267864514RNA PCR Primer, Index 17 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC15530.27268433408308357No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC11650.20455714694577742No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG8470.14872094717860385No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC7690.135025275537599No Hit
CTTAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC6380.11202357060206523No Hit
CTTATAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG6030.10587807691699896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT207.852282E-444.00096510
CCGTCTT22000.041.7045844
GCCGTCT25050.036.62677443
CTACGTG250.002352511335.1976833
CCGGTCG250.002352511335.197689
TGAGCGT250.002352511335.1976827
CTTTACA9150.032.728911
TTTACAC9250.032.343822
GATTCCC353.1988043E-431.456881
ATCAACG353.2169843E-431.4265027
CTATACA4800.031.194741
TTACACA9800.030.7530773
GGTACAT454.0409068E-529.3597561
CTCGCTA901.6370905E-1129.33397713
TTATACA44600.027.8681182
GTATACC406.99064E-427.5247731
CTTATAC46450.027.210711
GATCTTT508.282929E-526.423781
TATACAC49700.025.2297273
CTTTATA1750.025.1412032
Produced by FastQC (version 0.11.5)