FastQCFastQC Report
Wed 25 May 2016
SRR1780496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61513
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAA150.002215591769.9943134
ATGGAAT200.006923172652.4957359
CGTTGGT200.006923172652.4957339
TGCTAAC200.006923172652.4957341
GCTCACA400.0024026934.99715429
AGTCACA450.00427883331.10858213
ACAGGTC450.00427883331.1085828
GTGTAGG500.006964007428.1579741
CAATACA500.007161095727.9977254
CTTTACA700.001174165925.1410471
AATACAC700.001213974424.9979675
GTATAAT750.001754953423.4649751
AGCCCTA950.007138544718.41955619
ACGAATG1401.830856E-417.49857770
AACGAAT1553.98033E-415.80516669
GAACGAA1605.067248E-415.31125568
ACGAACG2203.8386E-412.72623770
CGACGAA2950.00444786959.49075368
GCTGCCG5906.172013E-89.49075370
GACGAAC3000.0051090889.33257569
Produced by FastQC (version 0.11.5)