##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780496_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61513 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.48905109489051 34.0 23.0 34.0 12.0 34.0 2 29.367109391510738 34.0 27.0 34.0 12.0 34.0 3 29.521190642628387 34.0 31.0 34.0 11.0 34.0 4 29.40906800188578 34.0 31.0 34.0 11.0 34.0 5 29.706289727374703 34.0 31.0 34.0 11.0 34.0 6 32.50418610700177 37.0 31.0 38.0 11.0 38.0 7 31.693316859850764 37.0 27.0 38.0 11.0 38.0 8 30.864093768796838 37.0 26.0 38.0 11.0 38.0 9 31.528961357761773 37.0 27.0 38.0 12.0 38.0 10-11 31.657901581779463 37.0 27.5 38.0 11.0 38.0 12-13 31.96328418383106 37.0 29.5 38.0 11.0 38.0 14-15 31.59652431193406 37.0 27.0 38.0 11.0 38.0 16-17 31.396566579422235 37.0 27.0 38.0 11.0 38.0 18-19 31.429779071090664 37.0 27.0 38.0 11.0 38.0 20-21 31.50097540357323 37.0 27.0 38.0 11.0 38.0 22-23 31.387267732024124 37.0 27.0 38.0 11.0 38.0 24-25 31.700299123762456 37.0 27.5 38.0 11.0 38.0 26-27 31.688634922699265 37.0 27.5 38.0 11.0 38.0 28-29 31.672557020467217 37.0 27.0 38.0 11.0 38.0 30-31 31.546128460650593 37.0 27.0 38.0 11.0 38.0 32-33 31.428120885016178 37.0 27.0 38.0 11.0 38.0 34-35 31.356696958366523 37.0 27.0 38.0 11.0 38.0 36-37 31.341919594232113 37.0 27.0 38.0 11.0 38.0 38-39 31.624445239217728 37.0 27.0 38.0 11.0 38.0 40-41 31.28039601385073 37.0 26.5 38.0 11.0 38.0 42-43 31.594215856810756 37.0 27.0 38.0 11.0 38.0 44-45 30.8163396355242 37.0 26.0 38.0 11.0 38.0 46-47 31.43309544323964 37.0 27.0 38.0 11.0 38.0 48-49 31.310357160275064 37.0 27.0 38.0 11.0 38.0 50-51 31.27378765464211 37.0 26.5 38.0 11.0 38.0 52-53 31.267236193975258 37.0 27.0 38.0 11.0 38.0 54-55 31.059954806301107 37.0 26.0 38.0 11.0 38.0 56-57 31.029766065709687 37.0 26.0 38.0 11.0 38.0 58-59 30.90566221774259 36.5 26.5 38.0 11.0 38.0 60-61 31.00612878578512 37.0 26.0 38.0 11.0 38.0 62-63 30.993342870612715 37.0 26.0 38.0 11.0 38.0 64-65 31.033196234942206 37.0 26.0 38.0 11.0 38.0 66-67 31.10710744070359 37.0 26.0 38.0 11.0 38.0 68-69 30.711971453188756 37.0 25.5 38.0 11.0 38.0 70-71 30.698250776258675 36.0 25.0 38.0 11.0 38.0 72-73 30.65572318046592 36.5 25.0 38.0 11.0 38.0 74-75 30.260099491164468 36.0 24.0 38.0 11.0 38.0 76 29.4186919838083 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.050432749731268 1101 2 -0.7706082911374672 1101 3 -0.006961979685101483 1101 4 -1.3096226036305012 1101 5 -1.4130238390549579 1101 6 -1.6075875292388346 1101 7 -1.7284605737384275 1101 8 -0.12620792007079373 1101 9 -1.264309679704084 1101 10-11 -1.2871306344522253 1101 12-13 -1.03048604819395 1101 14-15 -0.1113728196080892 1101 16-17 -1.3296842859684723 1101 18-19 -0.11092501938405519 1101 20-21 -1.0302446892231494 1101 22-23 -0.12145366316346795 1101 24-25 -1.2888415023533035 1101 26-27 -0.8417105636963313 1101 28-29 -0.26577170576149456 1101 30-31 -0.9996527540918976 1101 32-33 -0.685559452546606 1101 34-35 -0.5061290315264557 1101 36-37 -0.205469475763568 1101 38-39 -0.6462744071020019 1101 40-41 -0.4723347638321407 1101 42-43 -0.3035812716329538 1101 44-45 -0.861693223286025 1101 46-47 0.009915596736259857 1101 48-49 -0.8092059150169142 1101 50-51 -0.7146259345271062 1101 52-53 -0.30429140674228705 1101 54-55 -1.785391487037213 1101 56-57 -0.7118260047985245 1101 58-59 -0.045435782129331415 1101 60-61 0.007630463707251067 1101 62-63 -1.8402300185310345 1101 64-65 -1.2734231689348796 1101 66-67 -1.1961659428055462 1101 68-69 0.11692558013971421 1101 70-71 -0.0835039842553762 1101 72-73 -0.24276539210727677 1101 74-75 -1.0945341315137824 1101 76 -2.0811111061914964 1102 1 0.749170424871906 1102 2 0.4595504390212639 1102 3 1.1001808774577562 1102 4 0.6268853328774355 1102 5 1.0135634625323462 1102 6 0.2604680263167225 1102 7 1.3092378389599837 1102 8 1.4124825561196808 1102 9 1.7475950822006787 1102 10-11 0.39144079411920174 1102 12-13 -3.077609064066962 1102 14-15 -0.6659363116715831 1102 16-17 -1.5781961907303739 1102 18-19 -0.613901209860245 1102 20-21 0.46231483458637257 1102 22-23 -0.6601441393539424 1102 24-25 0.3768331008212975 1102 26-27 -3.5778216748074385 1102 28-29 0.16726400852422074 1102 30-31 0.42048613479698815 1102 32-33 -4.957880881118033 1102 34-35 -0.5626766505740761 1102 36-37 0.8952249686808749 1102 38-39 0.5799160690884726 1102 40-41 0.1625858710884991 1102 42-43 0.6135814267797421 1102 44-45 -4.4003836994764995 1102 46-47 -0.018358212787550343 1102 48-49 -0.1613884546994555 1102 50-51 -5.111947363098537 1102 52-53 -0.6683787083295911 1102 54-55 0.5752235923278697 1102 56-57 -1.432560131782651 1102 58-59 1.1361118369182854 1102 60-61 0.7739995113262985 1102 62-63 -4.746480018531031 1102 64-65 -1.1513993594110694 1102 66-67 0.15800072386111808 1102 68-69 -5.734264896050764 1102 70-71 -0.24372223822362926 1102 72-73 0.40802825868637527 1102 74-75 -6.372311909291557 1102 76 -1.8509523760327653 1103 1 -0.07106767036619033 1103 2 0.3662171056879302 1103 3 -0.32791436063748236 1103 4 0.5566472376393428 1103 5 0.24975393872282226 1103 6 0.18403945488815054 1103 7 0.67733307705522 1103 8 1.2491492227863468 1103 9 0.6947379393435362 1103 10-11 0.7934646036430095 1103 12-13 -0.5436804926383907 1103 14-15 -0.35736488310015346 1103 16-17 -0.06652952406370716 1103 18-19 0.16240831394927824 1103 20-21 -0.06137564160410136 1103 22-23 0.34176062255081874 1103 24-25 0.5273092912974882 1103 26-27 -0.4618692938550595 1103 28-29 1.0986925799527967 1103 30-31 0.22227184908270203 1103 32-33 -4.678118976356128 1103 34-35 -0.004581412478838587 1103 36-37 0.5527249686808737 1103 38-39 1.0501541643265675 1103 40-41 1.0361572996599229 1103 42-43 0.8063195220178407 1103 44-45 -3.7464551280479306 1103 46-47 0.264260834831493 1103 48-49 0.732540116729119 1103 50-51 0.17340977975860739 1103 52-53 0.40685938690850776 1103 54-55 0.6974854970897724 1103 56-57 1.1972017729792555 1103 58-59 1.194802313108763 1103 60-61 1.3475709398977322 1103 62-63 0.9664961719451561 1103 64-65 1.0995530215413112 1103 66-67 0.7444292952896916 1103 68-69 -2.4558125150983834 1103 70-71 0.21223014272875318 1103 72-73 0.8382663539244675 1103 74-75 -0.9814785759582243 1103 76 0.8835714334910421 1104 1 -0.6934799885868905 1104 2 -0.5846468806251579 1104 3 -0.2634490056332055 1104 4 -0.1350550720270398 1104 5 -0.4563538457083176 1104 6 -0.45435233296471367 1104 7 0.03343230716642864 1104 8 0.8030585469950751 1104 9 -0.7423535918797342 1104 10-11 -0.19269450670771704 1104 12-13 -1.0035607321593503 1104 14-15 -0.6253032919966479 1104 16-17 -1.251029096347711 1104 18-19 -0.19076533874532942 1104 20-21 0.16218295548742745 1104 22-23 -0.13006144759460625 1104 24-25 -0.07597556755623103 1104 26-27 -0.6499274632305969 1104 28-29 -0.5679797895938279 1104 30-31 -0.7213551825682814 1104 32-33 0.3287244795891269 1104 34-35 0.039404900609273597 1104 36-37 -0.002604372636490382 1104 38-39 -0.03624446698224304 1104 40-41 0.46337714568216626 1104 42-43 0.2605196931042393 1104 44-45 -3.8297570955415132 1104 46-47 -1.0773473773156361 1104 48-49 -0.29307151714598945 1104 50-51 0.02283664032319166 1104 52-53 -0.018820681526054983 1104 54-55 -0.18473862609243596 1104 56-57 0.01935746160200935 1104 58-59 -0.011254145402787685 1104 60-61 -0.8931305143366615 1104 62-63 -0.05423949956895413 1104 64-65 0.12839818835055183 1104 66-67 -0.38017292883349185 1104 68-69 -3.370474619461085 1104 70-71 -1.5053062131309574 1104 72-73 -0.2423495571106038 1104 74-75 -1.1164486358384664 1104 76 -0.38092813879296017 1105 1 -0.15502616996714735 1105 2 0.1477653898060467 1105 3 -0.4250252943964625 1105 4 0.759201108349636 1105 5 0.4227148325775687 1105 6 0.9594903726854369 1105 7 1.9553538272547435 1105 8 1.2645043704319967 1105 9 0.993604659215837 1105 10-11 1.2939913394770102 1105 12-13 -0.3375352412417456 1105 14-15 1.322092419373913 1105 16-17 1.357524745688881 1105 18-19 1.3927993754017933 1105 20-21 0.6206514932721987 1105 22-23 1.003197174825356 1105 24-25 0.8618025075784139 1105 26-27 1.271107561691629 1105 28-29 0.3943190763614126 1105 30-31 0.9068688163612322 1105 32-33 1.517012707602376 1105 34-35 0.8841177096281072 1105 36-37 1.4891495496864557 1105 38-39 0.9312395593784437 1105 40-41 0.7153592150629571 1105 42-43 0.8374607829915028 1105 44-45 1.8049734433806428 1105 46-47 0.9222177382632601 1105 48-49 0.9218617448216975 1105 50-51 0.8037050710594826 1105 52-53 0.0900676869883128 1105 54-55 -0.21778984209618102 1105 56-57 0.5608889238172416 1105 58-59 0.5199739012971101 1105 60-61 0.3858311154763392 1105 62-63 0.9119231472045364 1105 64-65 0.3598403319962209 1105 66-67 0.6810454165985504 1105 68-69 1.808848298947904 1105 70-71 1.3989021299593993 1105 72-73 1.353525731418486 1105 74-75 -0.6405847212096205 1105 76 -0.3729329559742389 1106 1 0.20928947249095486 1106 2 -0.3469971800263565 1106 3 -0.48612864635176933 1106 4 -0.6665670480749455 1106 5 -0.022746061277178597 1106 6 -1.202031973683276 1106 7 0.10001164848379318 1106 8 -0.45745792007079444 1106 9 -0.08097634637075046 1106 10-11 -0.5142139677855617 1106 12-13 0.4625695073616072 1106 14-15 0.12334940261413152 1106 16-17 0.1817740473648648 1106 18-19 -0.2213416860507209 1106 20-21 -0.4969113558898144 1106 22-23 -0.5464536631634687 1106 24-25 0.11150571986891933 1106 26-27 -0.3174050081407742 1106 28-29 -0.17202170576149456 1106 30-31 -0.4045138652030147 1106 32-33 0.5383988807867297 1106 34-35 -0.01967069819312428 1106 36-37 0.7764749686808727 1106 38-39 0.12872559289799668 1106 40-41 0.5023180139456365 1106 42-43 0.09676595058926907 1106 44-45 1.0299734433806442 1106 46-47 0.720332263402927 1106 48-49 0.2921829738719737 1106 50-51 0.4291240654728945 1106 52-53 -0.5289441845200642 1106 54-55 -0.8767109314816572 1106 56-57 0.6847017729792526 1106 58-59 -0.30689411546266854 1106 60-61 0.6440887970405846 1106 62-63 0.349353314802304 1106 64-65 0.5567851643984554 1106 66-67 1.014250723861121 1106 68-69 0.6190089134730492 1106 70-71 0.6626765713001781 1106 72-73 0.1398734967816111 1106 74-75 -1.6952285759582253 1106 76 0.18000000491961288 1107 1 -0.7115438608423794 1107 2 -0.35394162447079935 1107 3 0.0485935758704521 1107 4 -0.41795593696383193 1107 5 -0.0435793946105143 1107 6 0.7243569152056111 1107 7 -0.0701272404050961 1107 8 0.3974031910403184 1107 9 -0.4809763463707526 1107 10-11 -0.402408412230006 1107 12-13 0.0729861740282729 1107 14-15 -1.0099839307192013 1107 16-17 -0.12621206374624805 1107 18-19 -0.11092501938405519 1107 20-21 -0.1712169114453701 1107 22-23 -0.681175885385688 1107 24-25 -0.5846748356866378 1107 26-27 -0.26115500814077564 1107 28-29 -1.083132816872606 1107 30-31 -0.28368053186967757 1107 32-33 0.5484683252311733 1107 34-35 -0.054740142637566436 1107 36-37 -0.35338614243023514 1107 38-39 0.6787255928979938 1107 40-41 0.5804430139456365 1107 42-43 -0.10566460496628949 1107 44-45 -0.16724877884157863 1107 46-47 0.03005448562514701 1107 48-49 -0.2592059150169135 1107 50-51 0.7652351765840066 1107 52-53 0.5165419265910458 1107 54-55 0.03891406851834489 1107 56-57 -0.18057600479852454 1107 58-59 -0.16488022657377854 1107 60-61 -0.6208417585149739 1107 62-63 -0.8812022407532538 1107 64-65 0.17796571995401322 1107 66-67 0.08022294608334235 1107 68-69 0.2044255801397128 1107 70-71 -0.3314206509220412 1107 72-73 -0.8288765032183889 1107 74-75 -0.5653674648471139 1107 76 -1.7894444395248286 1108 1 -0.11678195608047659 1108 2 0.5433599628307881 1108 3 0.2720856393625155 1108 4 0.45379009478219956 1108 5 0.2726110815799636 1108 6 0.6411823120310061 1108 7 1.2659045056266542 1108 8 1.0034349370720648 1108 9 0.8147379393435337 1108 10-11 0.6677503179287285 1108 12-13 1.199176650218746 1108 14-15 1.2626351168998475 1108 16-17 0.0191847616505747 1108 18-19 0.7938368853778499 1108 20-21 1.3729100726816128 1108 22-23 0.7960463368365325 1108 24-25 -0.046976422988226574 1108 26-27 0.3867021347163657 1108 28-29 0.7729782942385057 1108 30-31 1.6994147062255607 1108 32-33 1.0447381665010198 1108 34-35 0.30970430180687814 1108 36-37 0.7012963972523032 1108 38-39 1.3672970214694224 1108 40-41 1.1704430139456328 1108 42-43 1.4520338077321249 1108 44-45 0.9364020148092145 1108 46-47 1.3756894062600686 1108 48-49 1.3011115453005502 1108 50-51 1.439124065472896 1108 52-53 0.8440022440513637 1108 54-55 1.1946283542326235 1108 56-57 0.7057732015506843 1108 58-59 0.07480231310876206 1108 60-61 1.104713797040585 1108 62-63 1.8436390290880134 1108 64-65 1.44812445011274 1108 66-67 0.887286438146834 1108 68-69 2.0527589134730455 1108 70-71 2.2150872855858914 1108 72-73 1.4954092110673258 1108 74-75 2.1099499954703447 1108 76 2.0721428620624707 1109 1 -0.10277700149748625 1109 2 -0.4138324401419631 1109 3 0.577816440353434 1109 4 0.5067711022140742 1109 5 0.8539157884338024 1109 6 1.5603235176462036 1109 7 0.7098021109115429 1109 8 1.955078207096836 1109 9 1.0892548675020812 1109 10-11 0.8755620437751332 1109 12-13 0.5967819351072734 1109 14-15 1.1495054719782978 1109 16-17 0.3542632092145759 1109 18-19 1.1877262330244278 1109 20-21 1.338673904225793 1109 22-23 1.0703815969521422 1109 24-25 1.3224703152446402 1109 26-27 1.3887004831887069 1109 28-29 0.7391054618685615 1109 30-31 1.183130643467507 1109 32-33 1.2108157883011756 1109 34-35 1.074675400072774 1109 36-37 0.4974648530739394 1109 38-39 0.7417313732448143 1109 40-41 1.4795759619225173 1109 42-43 1.2800932627279913 1109 44-45 0.3685572584095418 1109 46-47 0.6652022055994564 1109 48-49 0.9250586964153271 1109 50-51 1.1652512331029499 1109 52-53 1.6646793703932303 1109 54-55 0.8501857448189227 1109 56-57 0.7012480157538192 1109 58-59 1.3762886880055447 1109 60-61 1.751187785479896 1109 62-63 0.8285440662473889 1109 64-65 1.240907274225048 1109 66-67 0.8769313596992667 1109 68-69 1.6688282776348977 1109 70-71 1.588200415230812 1109 72-73 1.2378865025619596 1109 74-75 2.057480961614033 1109 76 1.1174277505843548 1110 1 1.022979948681428 1110 2 0.44764567711650116 1110 3 -0.530771503494627 1110 4 -0.2659718099797068 1110 5 0.1385634625323462 1110 6 -0.3109605451118469 1110 7 0.10090450562665154 1110 8 1.043434937072064 1110 9 -0.710738251132657 1110 10-11 -1.0787973011188967 1110 12-13 -0.2799900164479183 1110 14-15 -0.15403154976682032 1110 16-17 -0.18236285739704172 1110 18-19 0.3593130758540397 1110 20-21 0.30160054887208787 1110 22-23 0.24164157493177285 1110 24-25 0.11195214844034851 1110 26-27 -1.3784169129026793 1110 28-29 0.06309734185755289 1110 30-31 -0.13308529377444245 1110 32-33 1.125452452215299 1110 34-35 0.25279953990211723 1110 36-37 -0.17025122179531493 1110 38-39 -0.3248458356734325 1110 40-41 0.1715144425170685 1110 42-43 0.7683433315416472 1110 44-45 1.1115210624282632 1110 46-47 -0.20585821278755034 1110 48-49 0.5082544024434057 1110 50-51 0.5041240654728938 1110 52-53 -0.7844501369010182 1110 54-55 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2116 38-39 0.07366230175875543 2116 40-41 0.6551265582494317 2116 42-43 0.6128656341335734 2116 44-45 1.8176316712287424 2116 46-47 0.5733385925168477 2116 48-49 1.1398095561504604 2116 50-51 0.46456710344757823 2116 52-53 1.02948144839133 2116 54-55 -0.06488339983608782 2116 56-57 0.3756827856374798 2116 58-59 -0.022756457234820004 2116 60-61 -0.31845075992144345 2116 62-63 1.614543188220022 2116 64-65 0.8787392783225094 2116 66-67 0.6880640150003607 2116 68-69 0.14567030587810947 2116 70-71 -2.100337669206148 2116 72-73 0.7458070410854098 2116 74-75 -0.6141368038063248 2116 76 -0.8386708811563359 2117 1 -0.09291640986198857 2117 2 1.206515891869067 2117 3 1.7087242948247017 2117 4 0.23694602382047947 2117 5 -0.2488081527804482 2117 6 -0.20766922858523884 2117 7 -1.1737220116469267 2117 8 -1.1535772011165442 2117 9 0.8108537189887279 2117 10-11 0.9289641367896024 2117 12-13 1.3046855204335088 2117 14-15 0.8261271803919108 2117 16-17 1.67705591010996 2117 18-19 1.0459377257139906 2117 20-21 0.8947961604500492 2117 22-23 1.5415365329149644 2117 24-25 0.9468611120257826 2117 26-27 1.284106429767725 2117 28-29 1.6490567256110609 2117 30-31 1.1341299256466613 2117 32-33 1.554840874250786 2117 34-35 1.1772860011532806 2117 36-37 -0.3829613058289283 2117 38-39 1.3545425863620544 2117 40-41 -0.5564850906295256 2117 42-43 0.09123081987031512 2117 44-45 1.389940763642084 2117 46-47 0.6465577535990015 2117 48-49 1.7577875490353776 2117 50-51 1.6470979216820467 2117 52-53 1.8162151292054247 2117 54-55 -0.3117395262528966 2117 56-57 0.20831288409036475 2117 58-59 0.17433545970073183 2117 60-61 1.511511182661497 2117 62-63 0.2931441644755033 2117 64-65 0.9753513408690431 2117 66-67 0.14084386111602143 2117 68-69 0.7023013971331764 2117 70-71 1.4440695451563919 2117 72-73 1.1126267647554684 2117 74-75 0.7057109665254373 2117 76 -0.2796405179562029 2118 1 1.6779623119971276 2118 2 1.3713670175045074 2118 3 0.7090874030309458 2118 4 1.0869823346411067 2118 5 0.724321839957387 2118 6 0.7712704954525265 2118 7 1.0539468336689772 2118 8 -2.6100042163670913 2118 9 -1.1223343710621094 2118 10-11 -0.07030964679790586 2118 12-13 0.8557022234109937 2118 14-15 0.26439878533017946 2118 16-17 0.41761509674757846 2118 18-19 -0.33932008111244727 2118 20-21 -1.5113403682354942 2118 22-23 0.0012315220217153922 2118 24-25 0.9866214606096584 2118 26-27 0.6027338807481186 2118 28-29 0.030138788065666944 2118 30-31 -0.10497682816597376 2118 32-33 0.30556709066327414 2118 34-35 1.3248894869920669 2118 36-37 0.906799042754951 2118 38-39 0.35218238302145366 2118 40-41 -1.0942483440790518 2118 42-43 0.34032304935470137 2118 44-45 0.8018870236275539 2118 46-47 -0.052970205732879805 2118 48-49 0.07659655411888622 2118 50-51 0.1291240654728938 2118 52-53 -0.5612358511867299 2118 54-55 0.5845930808640212 2118 56-57 1.1218931310039437 2118 58-59 2.0425271808336305 2118 60-61 -1.2288664498729993 2118 62-63 0.06910640122205436 2118 64-65 0.2273484360033926 2118 66-67 -1.1707029798425843 2118 68-69 0.7288082961890971 2118 70-71 -0.09006262623068295 2118 72-73 -0.4819629229714728 2118 74-75 0.5404967326837493 2118 76 -0.11043209384581942 2119 1 -0.3622241329512228 2119 2 0.704448398204935 2119 3 0.3637863196346238 2119 4 0.6056268294760763 2119 5 0.6802301291990105 2119 6 0.8327469378813461 2119 7 -0.8761363106998807 2119 8 0.131870311221725 2119 9 0.22208487811904476 2119 10-11 1.169927188677029 2119 12-13 0.831829711443234 2119 14-15 0.33746504887263384 2119 16-17 0.23387863920159901 2119 18-19 0.21220423231662977 2119 20-21 -0.6707633967061426 2119 22-23 1.183393275612044 2119 24-25 0.4482616722498669 2119 26-27 0.9740490734918765 2119 28-29 1.5346109472997256 2119 30-31 0.7848568830963103 2119 32-33 1.0939898671812927 2119 34-35 0.8012689276572154 2119 36-37 0.3871467373883597 2119 38-39 0.7644398786122899 2119 40-41 1.1685382520408787 2119 42-43 0.6259113587525356 2119 44-45 -0.09638710083704538 2119 46-47 0.6695669572804768 2119 48-49 0.17662174938217845 2119 50-51 0.4777635212552056 2119 52-53 0.2703968018744902 2119 54-55 0.19979842225984257 2119 56-57 1.4577459906663321 2119 58-59 1.1850063947414142 2119 60-61 1.194917878673234 2119 62-63 0.5949315460948164 2119 64-65 0.5271720691603612 2119 66-67 0.240483717058396 2119 68-69 -0.0620710184997435 2119 70-71 0.17209408830698436 2119 72-73 -0.6149309249870925 2119 74-75 0.9155282267628628 2119 76 0.9475170117223364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 32.0 13 220.0 14 612.0 15 1161.0 16 1526.0 17 1854.0 18 2079.0 19 1890.0 20 1810.0 21 1343.0 22 983.0 23 798.0 24 710.0 25 691.0 26 692.0 27 779.0 28 903.0 29 1057.0 30 1332.0 31 1623.0 32 2082.0 33 2851.0 34 3949.0 35 5950.0 36 10366.0 37 14215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.18437765992274 28.971060040594516 9.962024487657958 27.882537811824786 2 16.733048298733603 30.487864353876414 30.061938126900007 22.717149220489976 3 15.356103587859476 26.394420691561134 27.69170744395494 30.557768276624454 4 14.262025913221596 22.224570416009623 33.54900590119162 29.96439776957716 5 10.867621478386683 39.347780144034594 31.780274088404077 18.004324289174644 6 21.067091509111897 42.26423682798758 17.459723960788775 19.208947702111747 7 21.395477378765467 33.90827955066409 22.679758750182888 22.016484320387562 8 19.33737583925349 35.08201518378229 22.070131516915122 23.510477460049096 9 20.277014614796872 21.81327524263164 20.59239510347406 37.31731503909742 10-11 20.411945442426806 27.543771235348625 27.291791978931286 24.752491343293286 12-13 20.14045811454489 25.55069660071855 26.396859200494205 27.911986084242358 14-15 19.255279371840096 26.938208183635982 23.360102742509714 30.44640970201421 16-17 20.717571895371712 29.06946499114008 24.33631915204916 25.876643961439044 18-19 19.34387852974168 27.870531432380147 25.0101604538878 27.775429583990373 20-21 19.14717214247395 28.112756653065208 25.123957537431114 27.616113667029733 22-23 21.283305967844196 27.74291613154943 24.527335685139725 26.446442215466647 24-25 19.916115292702354 29.655519971388163 24.177003235088517 26.251361500820963 26-27 18.90494692178889 30.08794888885276 25.501926422057124 25.50517776730122 28-29 20.20792352835986 28.509420772844763 26.762635540454866 24.520020158340515 30-31 20.20548501942679 27.98189000699039 26.775640921431243 25.036984052151578 32-33 18.608261668265243 30.403329377529953 26.227789247801276 24.760619706403524 34-35 19.652756327930682 28.93128281826606 26.400110545738297 25.015850308064962 36-37 20.02097117682441 28.000585242143938 26.628517549135957 25.3499260318957 38-39 19.690959634548793 27.0349357046478 28.07699185538016 25.197112805423245 40-41 18.998423097556614 26.7398761237462 27.828263944206917 26.43343683449027 42-43 17.792986848308487 26.22128655731309 28.785785118592816 27.199941475785604 44-45 17.684066782631312 26.7398761237462 29.326321265423573 26.249735828198915 46-47 18.06040999463528 27.33974932128168 28.32490693024239 26.274933753840653 48-49 17.293905353339944 28.338725147529793 27.968884626014013 26.398484873116253 50-51 17.567018353843903 27.713654024352575 27.006486433761967 27.712841188041555 52-53 16.822460292946207 27.843707834116366 27.65025279209273 27.6835790808447 54-55 16.445304244631217 28.002210914765985 27.28691496106514 28.26556987953766 56-57 17.267081755076163 27.655129809958872 26.96909596345488 28.10869247151009 58-59 17.245948010989547 27.45598491375807 26.836603644758018 28.461463430494366 60-61 16.93625737648952 26.727683579080846 26.669972200998167 29.666086843431472 62-63 17.168728561442297 27.012176287939134 26.430185489246178 29.38890966137239 64-65 17.931169021182512 27.54864825321477 25.223123567376003 29.29705915822672 66-67 17.52393802935965 28.9223416188448 24.427356818883812 29.126363532911743 68-69 17.493050249540747 28.928844309332984 24.016874481816853 29.561230959309416 70-71 17.895404223497472 30.14240892169135 23.395054703883734 28.56713215092745 72-73 18.49040040316681 29.490514200250356 23.107310649781347 28.911774746801488 74-75 18.260367727147106 29.492139872872404 23.925023978671174 28.322468421309317 76 19.100027636434575 28.296457659356562 24.560661973891698 28.04285273031717 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.5 11 3.0 12 3.0 13 4.0 14 3.5 15 4.5 16 9.0 17 11.0 18 14.0 19 17.0 20 25.0 21 33.0 22 37.5 23 60.0 24 103.5 25 129.0 26 160.0 27 226.0 28 315.5 29 370.0 30 468.5 31 627.5 32 829.0 33 970.0 34 1063.0 35 1373.0 36 1770.0 37 1950.0 38 2182.5 39 2619.5 40 3210.5 41 3792.0 42 3987.0 43 4058.5 44 4316.0 45 4463.0 46 4424.0 47 4437.5 48 4323.0 49 3946.5 50 3698.0 51 3540.5 52 3035.5 53 2443.5 54 2199.0 55 2043.0 56 1621.0 57 1200.5 58 1046.0 59 895.5 60 655.0 61 477.0 62 389.0 63 333.0 64 239.0 65 163.5 66 116.0 67 106.0 68 84.5 69 50.5 70 31.5 71 25.0 72 21.0 73 14.0 74 12.5 75 14.0 76 9.5 77 5.5 78 7.5 79 9.0 80 8.0 81 4.0 82 4.0 83 7.0 84 5.5 85 3.0 86 3.5 87 5.0 88 3.5 89 2.5 90 2.0 91 1.5 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 1.5 98 4.0 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6844081738819436 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 61513.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.629184074911 #Duplication Level Percentage of deduplicated Percentage of total 1 81.04877308491513 56.433599401752474 2 9.623870561042235 13.402045096158535 3 3.994770143120637 8.34457756896916 4 1.9798743900445936 5.514281533984686 5 1.2187434334944316 4.243005543543641 6 0.7587961990147324 3.1700616129923755 7 0.44593868926711955 2.1735242956773364 8 0.28950993439331324 1.6126672410709932 9 0.19845439051154537 1.2436395558662396 >10 0.4412691741962597 3.8625981499845565 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.019508071464568465 0.0 2 0.0 0.0 0.0 0.019508071464568465 0.0 3 0.0 0.0 0.0 0.02113374408661584 0.0 4 0.0 0.0 0.0 0.02113374408661584 0.0 5 0.0 0.0 0.0 0.026010761952757953 0.0 6 0.0 0.0 0.0 0.026010761952757953 0.0 7 0.0 0.0 0.0 0.026010761952757953 0.0 8 0.0 0.0 0.0 0.026010761952757953 0.0 9 0.0 0.0 0.0 0.026010761952757953 0.0 10 0.0 0.0 0.0 0.026010761952757953 0.0 11 0.0 0.0 0.0 0.027636434574805326 0.0 12 0.0 0.0 0.0 0.03251345244094744 0.0 13 0.0 0.0 0.0 0.03251345244094744 0.0 14 0.0 0.0 0.0 0.043893160795279045 0.0 15 0.0 0.0 0.0 0.043893160795279045 0.0 16 0.0 0.0 0.0 0.04714450603937379 0.0 17 0.0 0.0 0.0 0.04714450603937379 0.0 18 0.0 0.0 0.0 0.05039585128346853 0.0 19 0.0 0.0 0.0 0.06177555963780014 0.0 20 0.0 0.0 0.0 0.0634012322598475 0.0 21 0.0 0.0 0.0 0.06827825012598963 0.0 22 0.0 0.0 0.0 0.06827825012598963 0.0 23 0.0 0.0 0.0 0.069903922748037 0.0 24 0.0 0.0 0.0 0.069903922748037 0.0 25 0.0 0.0 0.0 0.069903922748037 0.0 26 0.0 0.0 0.0 0.07478094061417911 0.0 27 0.0 0.0 0.0 0.08778632159055809 0.0 28 0.0 0.0 0.0 0.12029977403150553 0.0 29 0.0 0.0 0.0 0.14793620860631085 0.0 30 0.0 0.0 0.0 0.17232129793702144 0.0 31 0.0 0.0 0.0 0.20808609562206362 0.0 32 0.0 0.0 0.0 0.25848194690553217 0.0 33 0.0 0.0 0.0 0.3267601970315218 0.0 34 0.0 0.0 0.0 0.3836587388031798 0.0 35 0.0 0.0 0.0 0.5218409116772065 0.0 36 0.0 0.0 0.0 0.6779054833937541 0.0 37 0.0 0.0 0.0 0.9607725196299969 0.0 38 0.0 0.0 0.0 1.3428055858111294 0.0 39 0.0 0.0 0.0 1.8093736283387252 0.0 40 0.0 0.0 0.0 2.2824443613545102 0.0 41 0.0 0.0 0.0 2.909953993464796 0.0 42 0.0 0.0 0.0 3.5927364947246923 0.0 43 0.0 0.0 0.0 4.340545900866483 0.0 44 0.0 0.0 0.0 4.974558223464959 0.0 45 0.0 0.0 0.0 5.72887032009494 0.0 46 0.0 0.0 0.0 6.330369190252467 0.0 47 0.0 0.0 0.0 7.003397655780079 0.0 48 0.0 0.0 0.0 7.6487896867328855 0.0 49 0.0 0.0 0.0 8.284427681953408 0.0 50 0.0 0.0 0.0 8.973712873701494 0.0 51 0.0 0.0 0.0 9.633735958252727 0.0 52 0.0 0.0 0.0 10.279127989205534 0.0 53 0.0 0.0 0.0 10.895257912961489 0.0 54 0.0 0.0 0.0 11.584543104709574 0.0 55 0.0 0.0 0.0 12.184416302245054 0.0 56 0.0 0.0 0.0 12.742022011607302 0.0 57 0.0 0.0 0.0 13.254108887552224 0.0 58 0.0 0.0 0.0 13.811714596914474 0.0 59 0.0 0.0 0.0 14.398582413473575 0.0 60 0.0 0.0 0.0 14.913920634662592 0.0 61 0.0 0.0 0.0 15.47802903451303 0.0 62 0.0 0.0 0.0 16.014500999788662 0.0 63 0.0 0.0 0.0 16.61599986994619 0.0 64 0.0 0.0 0.0 17.181733942418674 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCAAA 15 0.0022155917 69.99431 34 ATGGAAT 20 0.0069231726 52.49573 59 CGTTGGT 20 0.0069231726 52.49573 39 TGCTAAC 20 0.0069231726 52.49573 41 GCTCACA 40 0.00240269 34.997154 29 AGTCACA 45 0.004278833 31.108582 13 ACAGGTC 45 0.004278833 31.108582 8 GTGTAGG 50 0.0069640074 28.157974 1 CAATACA 50 0.0071610957 27.997725 4 CTTTACA 70 0.0011741659 25.141047 1 AATACAC 70 0.0012139744 24.997967 5 GTATAAT 75 0.0017549534 23.464975 1 AGCCCTA 95 0.0071385447 18.419556 19 ACGAATG 140 1.830856E-4 17.498577 70 AACGAAT 155 3.98033E-4 15.805166 69 GAACGAA 160 5.067248E-4 15.311255 68 ACGAACG 220 3.8386E-4 12.726237 70 CGACGAA 295 0.0044478695 9.490753 68 GCTGCCG 590 6.172013E-8 9.490753 70 GACGAAC 300 0.005109088 9.332575 69 >>END_MODULE