FastQCFastQC Report
Wed 25 May 2016
SRR1780496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61513
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT33315.415115504039796No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT32255.242794206102775No Hit
CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC11041.794742574740299No Hit
CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC4700.7640661323622648No Hit
TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT2870.46656804252759576No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG2720.4421829531968852No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1810.29424674459057437No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1430.23247118495277422No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG1320.21458878611025312No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT1230.19995773251182675No Hit
CTTATAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG1190.19345504202363728No Hit
CTTAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC1080.17557264318111618No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC810.13167948238583713No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGT770.12517679189764766No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGT740.12029977403150553No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGTCTT710.11542275616536343No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGTCTT630.10241737518898444No Hit
CTTATTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG620.10079170256693708No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGATT150.002214877270.0000121
AGCATAA150.002214877270.0000123
GTCCCAC150.002214877270.000011
CCACCGT150.002214877270.000019
CTTTACA2000.063.0000041
TTTACAC2150.060.232562
CTATACA1050.059.9999961
TTACACA2400.053.9583363
GCTATAC200.006920947752.53
ATATGTA200.006920947752.531
AAGGAGT200.006920947752.520
ACGACCA200.006920947752.511
AGTCGCA200.006920947752.524
CCATTGT200.006920947752.514
GCATACC200.006920947752.51
TTAATAC305.835864E-446.6666683
TCTTTAT305.835864E-446.6666681
ATAACAC305.835864E-446.6666684
CTTATAA404.544027E-543.751
TAACACA602.546658E-740.8333365
Produced by FastQC (version 0.11.5)