##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780496_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61513 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76541544063856 34.0 34.0 34.0 31.0 34.0 2 33.30050558418546 34.0 34.0 34.0 32.0 34.0 3 33.447076227789246 34.0 34.0 34.0 34.0 34.0 4 33.49847999609839 34.0 34.0 34.0 34.0 34.0 5 33.540649943914296 34.0 34.0 34.0 34.0 34.0 6 37.301367190675144 38.0 38.0 38.0 36.0 38.0 7 36.96644611708094 38.0 37.0 38.0 35.0 38.0 8 36.973989238047245 38.0 37.0 38.0 35.0 38.0 9 36.70583453904053 38.0 37.0 38.0 34.0 38.0 10-11 36.46022791930161 38.0 37.0 38.0 34.0 38.0 12-13 36.29922943117715 38.0 37.0 38.0 34.0 38.0 14-15 36.49259506120657 38.0 37.0 38.0 34.0 38.0 16-17 36.576487896867334 38.0 37.0 38.0 34.0 38.0 18-19 36.62621722237576 38.0 37.0 38.0 34.0 38.0 20-21 36.4132947507031 38.0 37.0 38.0 34.0 38.0 22-23 36.45534277307236 38.0 37.0 38.0 34.0 38.0 24-25 35.73715312210427 38.0 36.5 38.0 31.5 38.0 26-27 35.956342561734914 38.0 37.0 38.0 32.0 38.0 28-29 35.8797896379627 38.0 37.0 38.0 31.5 38.0 30-31 35.53831710370166 38.0 36.0 38.0 31.5 38.0 32-33 35.95699283078373 38.0 37.0 38.0 33.0 38.0 34-35 35.761091151463916 38.0 36.5 38.0 31.5 38.0 36-37 35.76885373823419 38.0 37.0 38.0 31.5 38.0 38-39 35.256401085949314 38.0 36.0 38.0 29.0 38.0 40-41 35.22983759530506 37.5 35.5 38.0 29.0 38.0 42-43 35.800855103799194 38.0 36.5 38.0 32.5 38.0 44-45 36.01271275990441 38.0 37.0 38.0 33.0 38.0 46-47 36.213540227269036 38.0 37.0 38.0 34.0 38.0 48-49 35.981516102287316 38.0 37.0 38.0 32.5 38.0 50-51 36.09141969990083 38.0 37.0 38.0 33.5 38.0 52-53 35.9768910636776 38.0 37.0 38.0 33.0 38.0 54-55 36.15678799603336 38.0 37.0 38.0 34.0 38.0 56-57 36.14224635442915 38.0 37.0 38.0 34.0 38.0 58-59 36.16750930697576 38.0 37.0 38.0 34.0 38.0 60-61 35.923203225334476 38.0 37.0 38.0 32.5 38.0 62-63 36.26602506787184 38.0 37.0 38.0 34.0 38.0 64-65 36.23841301838635 38.0 37.0 38.0 34.0 38.0 66-67 35.347674475314165 38.0 36.0 38.0 30.5 38.0 68-69 33.91388812121015 37.5 34.0 38.0 22.5 38.0 70-71 33.038276461886106 37.0 32.5 38.0 16.0 38.0 72-73 32.059499617966935 37.0 29.0 38.0 11.0 38.0 74-75 32.43413587371775 37.0 31.5 38.0 11.0 38.0 76 32.0666200640515 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40228899907040017 1101 2 0.5421135316179786 1101 3 0.18068670077489202 1101 4 0.4001613113342657 1101 5 0.378094059861354 1101 6 -1.1317130764183219 1101 7 0.22858593302206742 1101 8 0.3519282978652143 1101 9 0.143071806680922 1101 10-11 -0.13417043766557413 1101 12-13 0.38585644622139625 1101 14-15 0.5470777193206473 1101 16-17 0.005424795612029243 1101 18-19 -0.5297820344540582 1101 20-21 -0.4458585489608424 1101 22-23 0.4095954939781592 1101 24-25 -1.3237317078028141 1101 26-27 -0.06755362844818791 1101 28-29 0.5672168993731574 1101 30-31 1.1365561928911276 1101 32-33 0.6150578345029274 1101 34-35 0.6674672306075351 1101 36-37 0.36736615965952524 1101 38-39 0.4953901605524038 1101 40-41 1.4432983670263582 1101 42-43 1.0870321570949173 1101 44-45 0.6634099241007476 1101 46-47 0.9501159838799751 1101 48-49 -1.5726210159624898 1101 50-51 -1.083173344285271 1101 52-53 -1.6207163507072124 1101 54-55 -0.8476022637216545 1101 56-57 -1.269931029706882 1101 58-59 -0.8059806804421044 1101 60-61 -0.9357079205252177 1101 62-63 -1.0270823040635904 1101 64-65 -1.0301465913776298 1101 66-67 -1.2059369096024497 1101 68-69 -1.21782636864085 1101 70-71 -0.8111169314210045 1101 72-73 -2.0909553056066557 1101 74-75 -0.5311625204186896 1101 76 -0.44189706135076534 1102 1 -0.8139808421994381 1102 2 0.31691511891956736 1102 3 0.23425812934632262 1102 4 0.3465898827628422 1102 5 -0.1536519718846776 1102 6 0.15697739977215264 1102 7 0.07283196476809906 1102 8 0.08903147246838472 1102 9 0.5646987908079026 1102 10-11 0.45759543535029934 1102 12-13 0.5326818430467881 1102 14-15 -0.12008497909205573 1102 16-17 0.11554384323107314 1102 18-19 -0.5530955265175521 1102 20-21 0.5010660542137586 1102 22-23 0.3143573987400643 1102 24-25 -5.2225412316123325 1102 26-27 -1.4266806125751756 1102 28-29 0.11682007397633498 1102 30-31 1.5383419071768358 1102 32-33 0.4860895805346743 1102 34-35 1.1347291353694402 1102 36-37 -2.1326338403404748 1102 38-39 -0.1415146013523625 1102 40-41 0.8545086844866745 1102 42-43 0.5785996174123724 1102 44-45 0.9406916701324946 1102 46-47 1.23979852356252 1102 48-49 -3.3365099048513756 1102 50-51 -0.3450781061900301 1102 52-53 0.16301380802295284 1102 54-55 0.4257112283418394 1102 56-57 0.9101284941026435 1102 58-59 0.49907884336741404 1102 60-61 -5.712989666556961 1102 62-63 -2.5826378596191475 1102 64-65 -0.2955235755046175 1102 66-67 1.1239242015086646 1102 68-69 -1.0516557337202173 1102 70-71 -0.5571486774527443 1102 72-73 -5.748197369098719 1102 74-75 -1.4656863299424963 1102 76 -0.22661928357298677 1103 1 1.0348286816100867 1103 2 0.5557246427290963 1103 3 0.13282955791775208 1103 4 0.3863517875247453 1103 5 0.29325279002008386 1103 6 0.33221549501025294 1103 7 0.7275938695300042 1103 8 0.5945076629445794 1103 9 0.24731783842695165 1103 10-11 0.42819067344554185 1103 12-13 -0.6375562521913025 1103 14-15 -0.4229421219491982 1103 16-17 0.03637717656440742 1103 18-19 0.48833304491102325 1103 20-21 -0.025600612452905125 1103 22-23 0.5750716844543504 1103 24-25 -3.208493612564716 1103 26-27 -0.35560918400374675 1103 28-29 0.4913438835001429 1103 30-31 1.2584609547958863 1103 32-33 1.2575181519632466 1103 34-35 0.7038958020361079 1103 36-37 -0.7245386022452394 1103 38-39 0.5899139700762106 1103 40-41 -0.058705601227615034 1103 42-43 -0.15997181115905335 1103 44-45 1.0606916701324849 1103 46-47 1.053727094991089 1103 48-49 -1.9210337143751914 1103 50-51 -0.17781620142812926 1103 52-53 0.2900376175467585 1103 54-55 0.11523503786564504 1103 56-57 0.5834618274359755 1103 58-59 -0.4603259185373503 1103 60-61 -3.977989666556958 1103 62-63 -0.8341854786667611 1103 64-65 0.12090499592395787 1103 66-67 -0.18179008420562326 1103 68-69 -0.12748906705355267 1103 70-71 -0.3157201060241803 1103 72-73 -4.168197369098721 1103 74-75 -2.2021149013710684 1103 76 -3.2730478550015576 1104 1 0.22747196646893997 1104 2 0.29656296608238364 1104 3 0.41796214987668634 1104 4 -0.37878080443419293 1104 5 -0.7859944298259407 1104 6 0.165816863701437 1104 7 0.47438599955566474 1104 8 0.5266205799676769 1104 9 0.7576343482644177 1104 10-11 -0.6295338774526584 1104 12-13 0.07356436371973274 1104 14-15 0.825167373345927 1104 16-17 0.40412739042240986 1104 18-19 0.6866392895645745 1104 20-21 0.19196996068652794 1104 22-23 0.13289888719173604 1104 24-25 0.46868346185787146 1104 26-27 1.0943309357567372 1104 28-29 1.5179820357670408 1104 30-31 0.9339613825118818 1104 32-33 0.3445155856672599 1104 34-35 0.04396423659555637 1104 36-37 1.565469952074693 1104 38-39 1.5774759888957135 1104 40-41 1.7863414475320099 1104 42-43 0.6942967944867959 1104 44-45 0.7781595914327539 1104 46-47 0.8647450590629475 1104 48-49 -0.16260770924260015 1104 50-51 0.4044763563135376 1104 52-53 0.37624805381707915 1104 54-55 -0.3734048252652329 1104 56-57 0.2336243595146783 1104 58-59 -0.9282985447135701 1104 60-61 0.5431052863942796 1104 62-63 0.4032653339936303 1104 64-65 0.8987664159410684 1104 66-67 0.025121806299083005 1104 68-69 0.0786016087377206 1104 70-71 0.5410121437619679 1104 72-73 0.456678593262275 1104 74-75 -0.6544160134326589 1104 76 -0.35910431351310734 1105 1 1.1349084900697832 1105 2 0.5313671008296481 1105 3 0.4551280415570176 1105 4 -0.01371205924301222 1105 5 -0.12788049010761426 1105 6 0.2953120632464845 1105 7 0.5775539653001545 1105 8 -0.5470086977896571 1105 9 0.8416673994804214 1105 10-11 0.1478554779148169 1105 12-13 -0.32263207022801055 1105 14-15 0.24902914700530943 1105 16-17 0.025443417585954364 1105 18-19 0.4949890704497264 1105 20-21 -0.22066844964364662 1105 22-23 0.3609455870880325 1105 24-25 0.7535494840035213 1105 26-27 0.4694187489571462 1105 28-29 0.812716589006925 1105 30-31 1.274358799967473 1105 32-33 0.9278294049560643 1105 34-35 -1.0203819314036409 1105 36-37 -0.03998952004252487 1105 38-39 -0.2995353515518815 1105 40-41 0.5959791553565879 1105 42-43 0.14823249849776943 1105 44-45 0.7092471370119782 1105 46-47 0.8513807262760054 1105 48-49 -0.13795310783089576 1105 50-51 0.6944104545974099 1105 52-53 0.5612746486720539 1105 54-55 0.38926536508033394 1105 56-57 0.3248664563266388 1105 58-59 0.10015293222083699 1105 60-61 0.8822816822060133 1105 62-63 -0.7955102990977281 1105 64-65 0.5066512050221235 1105 66-67 -0.28948361972038583 1105 68-69 0.5752563439600138 1105 70-71 1.1533461349973706 1105 72-73 1.076623061866961 1105 74-75 -0.1657062820574211 1105 76 0.2559226158683572 1106 1 -0.649099889818487 1106 2 0.4407246427290943 1106 3 0.3244367007748892 1106 4 0.1709946446676014 1106 5 0.06003850430579405 1106 6 0.14328692358167672 1106 7 -0.5179418447557111 1106 8 0.4366505200874329 1106 9 -1.2242893044301937 1106 10-11 0.473815673445543 1106 12-13 1.2271758906658334 1106 14-15 0.11027216376508875 1106 16-17 0.3720914622786964 1106 18-19 0.345565187768166 1106 20-21 0.15448867326137616 1106 22-23 0.40230382731149206 1106 24-25 -0.5508150411361399 1106 26-27 0.580015815996255 1106 28-29 0.5258974549287174 1106 30-31 0.9386395262244562 1106 32-33 0.782071723391816 1106 34-35 1.183092230607535 1106 36-37 1.0934078263261924 1106 38-39 0.6558068272190667 1106 40-41 0.9728122559152439 1106 42-43 1.1415460459838016 1106 44-45 0.9460488129896305 1106 46-47 1.1143520949910908 1106 48-49 1.5311984284819502 1106 50-51 -0.061298344285276585 1106 52-53 0.5497697604039047 1106 54-55 -0.3656578192772173 1106 56-57 0.38319397029312086 1106 58-59 0.8148526528912257 1106 60-61 0.7191531905858994 1106 62-63 0.5246538070475211 1106 64-65 -1.1294521469331897 1106 66-67 0.39892420150866315 1106 68-69 1.32349307580359 1106 70-71 -0.03750582030989591 1106 72-73 -0.19998308338443493 1106 74-75 -0.5947041870853553 1106 76 -1.0578692835729875 1107 1 0.8467334435148501 1107 2 -0.01205313504868144 1107 3 -0.1887577436695551 1107 4 0.10016131133426853 1107 5 0.2142051709724626 1107 6 0.375231368026121 1107 7 0.5244192663554017 1107 8 -0.5939050354681186 1107 9 -0.922205971096858 1107 10-11 0.4165240067788716 1107 12-13 0.09141200177694486 1107 14-15 0.23110549709842587 1107 16-17 0.6554247956120278 1107 18-19 0.5639679655459418 1107 20-21 0.7534470065947119 1107 22-23 -0.03762672824405655 1107 24-25 0.3373794033082973 1107 26-27 -0.005053628448187908 1107 28-29 0.5255502327064931 1107 30-31 0.1448895262244534 1107 32-33 1.0400578345029245 1107 34-35 0.23413389727419798 1107 36-37 1.1784772707706352 1107 38-39 0.3509457161079581 1107 40-41 0.6106594781374639 1107 42-43 0.9932821570949173 1107 44-45 0.8446599241007462 1107 46-47 1.0403937616577537 1107 48-49 0.7996012062597302 1107 50-51 0.9709933223813962 1107 52-53 0.4612280937372333 1107 54-55 0.4808699585005627 1107 56-57 0.8974300814042238 1107 58-59 0.6627693195578956 1107 60-61 1.1247087461414509 1107 62-63 1.204862140380861 1107 64-65 0.9108256308445917 1107 66-67 -0.15107579849134112 1107 68-69 0.7064791869146987 1107 70-71 -0.5833391536432231 1107 72-73 0.20696136106001006 1107 74-75 -0.9533847426409103 1107 76 0.13726960531590038 1108 1 -1.0165998898184867 1108 2 0.2185817855862382 1108 3 0.07568670077489514 1108 4 0.25492321609617363 1108 5 -0.06674720997991557 1108 6 -0.2677845049897485 1108 7 0.1161652981014285 1108 8 -0.822635194198277 1108 9 -0.2669678758587608 1108 10-11 0.3996192448741098 1108 12-13 0.8024437478086952 1108 14-15 0.4742007351936621 1108 16-17 -0.40647996629273564 1108 18-19 0.1454759020538816 1108 20-21 0.9401136732613793 1108 22-23 -0.13064260125992888 1108 24-25 0.9229349588638556 1108 26-27 -0.45275204114660283 1108 28-29 -0.7115132593569982 1108 30-31 0.1727466690815973 1108 32-33 1.051803866248953 1108 34-35 0.82103865917896 1108 36-37 1.3811756834690456 1108 38-39 1.5584853986476404 1108 40-41 0.5898658273438144 1108 42-43 -0.4228289540161967 1108 44-45 0.8521202415610603 1108 46-47 0.3965842378482378 1108 48-49 0.9161091427676666 1108 50-51 0.6050409414290101 1108 52-53 -0.6613909538818135 1108 54-55 -0.2904792478486371 1108 56-57 0.39774754172168514 1108 58-59 0.5882455100340849 1108 60-61 0.5877246191573278 1108 62-63 0.8115288070475231 1108 64-65 0.575190710209668 1108 66-67 1.186781344365805 1108 68-69 1.8953680758035958 1108 70-71 0.5614227511186769 1108 72-73 1.1003740594727063 1108 74-75 0.34359938434321435 1108 76 1.5612378592841551 1109 1 -1.4302415083155964 1109 2 0.3804645271221574 1109 3 0.45742080482113323 1109 4 0.2522807718352311 1109 5 0.22210497829423304 1109 6 -0.05909746948190531 1109 7 -0.24102710487132128 1109 8 0.5145406934978425 1109 9 0.968738152217206 1109 10-11 0.8203294017692428 1109 12-13 0.6359367288161266 1109 14-15 0.7510958631870537 1109 16-17 0.7168891501399699 1109 18-19 0.9458722686930159 1109 20-21 1.1056628062093594 1109 22-23 0.46185946314964355 1109 24-25 0.5307962305401546 1109 26-27 0.552830122354635 1109 28-29 -0.6893662733949881 1109 30-31 0.3745138036811042 1109 32-33 0.9064033707906631 1109 34-35 0.13639786644568375 1109 36-37 1.5470386066730129 1109 38-39 1.5876348619011438 1109 40-41 1.163292747244725 1109 42-43 1.300523647139876 1109 44-45 1.0257453447815408 1109 46-47 1.0384669793841539 1109 48-49 1.0755806539154804 1109 50-51 0.6296156730557669 1109 52-53 0.5268831997102623 1109 54-55 0.19890142927769716 1109 56-57 0.429671571449191 1109 58-59 0.25260554306463234 1109 60-61 1.1145289131292486 1109 62-63 0.6183496162960793 1109 64-65 1.258047853066813 1109 66-67 0.7135195772312102 1109 68-69 0.07109061915619463 1109 70-71 0.9488381103259442 1109 72-73 1.025956222973953 1109 74-75 0.1966794834348775 1109 76 1.3203460343460875 1110 1 -1.3080284612470585 1110 2 -0.32594202393757143 1110 3 0.16282955791774611 1110 4 0.2275422637152218 1110 5 0.2630146947819867 1110 6 0.008167875962627136 1110 7 0.28116529810143476 1110 8 0.6485552819921949 1110 9 0.9218416479507567 1110 10-11 -0.7834759932211313 1110 12-13 0.5356580335229779 1110 14-15 0.77574835424128 1110 16-17 0.43994860513583234 1110 18-19 0.10166637824435298 1110 20-21 0.8343993875470872 1110 22-23 -0.6677854584027898 1110 24-25 -0.8981364697075733 1110 26-27 1.4364146255200652 1110 28-29 0.4203915025477656 1110 30-31 0.30024666908159503 1110 32-33 1.0813276757727621 1110 34-35 1.2865148496551555 1110 36-37 1.697723302516664 1110 38-39 1.352533017695258 1110 40-41 -0.4371579821799969 1110 42-43 0.4089567602695183 1110 44-45 0.8990250034658231 1110 46-47 0.6713461426101404 1110 48-49 1.371823428481953 1110 50-51 -0.2617447728567015 1110 52-53 0.48741856992771204 1110 54-55 0.006068371198978184 1110 56-57 0.7940570655312129 1110 58-59 -0.8104449661563962 1110 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44-45 -0.34683093384580843 2116 46-47 -1.2053868290595418 2116 48-49 -0.09653100189779451 2116 50-51 -0.7738379012472976 2116 52-53 -0.1749533408619186 2116 54-55 -0.6060850344670854 2116 56-57 -0.6516557132511878 2116 58-59 -2.571935321792324 2116 60-61 -1.7371759233381567 2116 62-63 0.16076931337664035 2116 64-65 -2.355876197566097 2116 66-67 -1.1260757984913354 2116 68-69 -1.1052648355888124 2116 70-71 1.0088549391837773 2116 72-73 -0.14602738718190267 2116 74-75 -0.05225956683219124 2116 76 -1.0319990304084286 2117 1 0.8189556657370716 2117 2 0.5327181067813811 2117 3 0.13085009946770043 2117 4 0.03905020022315853 2117 5 0.13381301410971957 2117 6 0.3670614333856008 2117 7 0.7453342990351359 2117 8 0.5410622847933197 2117 9 -0.3744935527962028 2117 10-11 -0.04409690825380608 2117 12-13 -0.6247644688112857 2117 14-15 0.2188505951376456 2117 16-17 -0.8393464462180376 2117 18-19 -0.7424045834736646 2117 20-21 0.27044047064699583 2117 22-23 0.4251183697951504 2117 24-25 -1.0459539300250285 2117 26-27 -1.6643673539383812 2117 28-29 -0.6974889829797846 2117 30-31 0.40126207524406254 2117 32-33 -2.2337983746473995 2117 34-35 0.8414868384506704 2117 36-37 -0.9153135789025697 2117 38-39 0.3066646703563194 2117 40-41 0.4247117657191666 2117 42-43 0.09377235317334254 2117 44-45 -0.5602420366835688 2117 46-47 -0.557645454028517 2117 48-49 -0.49909160419778686 2117 50-51 -0.576228899840828 2117 52-53 0.6738097930836418 2117 54-55 -0.667659453264136 2117 56-57 0.3340313885937718 2117 58-59 -1.269420222925774 2117 60-61 -0.15404942379318953 2117 62-63 -0.6663796896844971 2117 64-65 -1.3334554149070428 2117 66-67 -1.1898012886874128 2117 68-69 0.3064791869147072 2117 70-71 0.08594189210841563 2117 72-73 1.5767326028900754 2117 74-75 1.849556433829683 2117 76 1.7480539190413893 2118 1 0.18932603610743826 2118 2 0.43177402544514365 2118 3 -0.5369058918177032 2118 4 0.10695143479105695 2118 5 0.21297060307122706 2118 6 0.020293096421184487 2118 7 0.7170118589479912 2118 8 0.8202924953960746 2118 9 -1.025909674800566 2118 10-11 -0.5226735240853202 2118 12-13 -1.2206250352600847 2118 14-15 0.6925252501848362 2118 16-17 -2.608772735252167 2118 18-19 -0.15362462704664637 2118 20-21 -0.16692336377565908 2118 22-23 0.8432374692868052 2118 24-25 1.062070761332997 2118 26-27 -2.376967208695099 2118 28-29 -0.12414112531819654 2118 30-31 -0.29554257254097394 2118 32-33 -0.05932488154645199 2118 34-35 0.13197340344704145 2118 36-37 -2.555473346513317 2118 38-39 0.10187164203388477 2118 40-41 0.1427582435695598 2118 42-43 0.6244549966010879 2118 44-45 -2.681883285775797 2118 46-47 0.09903573696639256 2118 48-49 0.7082431815683705 2118 50-51 -0.9589449492235431 2118 52-53 -0.9748830173738767 2118 54-55 -0.11882139952412274 2118 56-57 0.47582514313262436 2118 58-59 -0.27087265575075037 2118 60-61 -0.33269866126595105 2118 62-63 -0.7716810694956848 2118 64-65 0.41854168022730676 2118 66-67 0.41404765829879153 2118 68-69 -0.7243850106161602 2118 70-71 0.21666084635678118 2118 72-73 1.4294922252575404 2118 74-75 0.20340538081588022 2118 76 -0.04606372801742964 2119 1 0.6987742598413789 2119 2 -0.787676717815124 2119 3 0.3788159524755699 2119 4 -0.3056210015909002 2119 5 0.21624598729899702 2119 6 -0.2579885866224032 2119 7 -0.11594354543598229 2119 8 0.3585892955976391 2119 9 -0.19975699150501924 2119 10-11 -0.008816129275544426 2119 12-13 -0.07020931341578773 2119 14-15 -0.09287409473831332 2119 16-17 -0.21396295949001143 2119 18-19 -0.129626138762454 2119 20-21 -1.2608387076910006 2119 22-23 0.36962950758360336 2119 24-25 0.9789893806325622 2119 26-27 0.5303772105540787 2119 28-29 0.05814660458857901 2119 30-31 0.888256873163229 2119 32-33 0.8602392403986201 2119 34-35 0.5237597476143421 2119 36-37 -0.15814404442210872 2119 38-39 -1.3489975945496369 2119 40-41 -0.6663246488466612 2119 42-43 -0.028543239730481673 2119 44-45 0.06654201026854878 2119 46-47 0.15263865961694023 2119 48-49 -0.33055752389899595 2119 50-51 0.9963334584358137 2119 52-53 0.2603777536011762 2119 54-55 0.06856837119897818 2119 56-57 -1.0048374922919123 2119 58-59 0.2962727209184379 2119 60-61 0.41711237425936076 2119 62-63 0.10826350092507653 2119 64-65 -0.08889092244339025 2119 66-67 0.8858289634134238 2119 68-69 0.6749599125382915 2119 70-71 -0.26973371146635117 2119 72-73 1.619149569676786 2119 74-75 0.007953125839819108 2119 76 1.048040580372593 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 5.0 23 25.0 24 38.0 25 94.0 26 172.0 27 297.0 28 545.0 29 806.0 30 1270.0 31 2065.0 32 3011.0 33 4507.0 34 6711.0 35 8793.0 36 11811.0 37 21360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.960512412010466 23.414562775348298 11.026937395347325 32.59798741729391 2 16.40141108383594 18.171768569245526 44.81166582673581 20.615154520182728 3 15.3122104270642 26.84148066262416 36.439451823191845 21.406857087119796 4 11.012306341748898 24.01931299074992 35.24295677336498 29.7254238941362 5 10.11981207224489 39.56562027538894 37.27342187830215 13.04114577406402 6 20.76146505616699 40.165493472924425 17.089070602961975 21.98397086794661 7 21.1565035033245 36.185846894152455 19.73403995903305 22.923609643489996 8 18.55705298067075 36.21998601921545 24.05020077056882 21.172760229544973 9 18.90494692178889 19.327621803521204 18.34083852193845 43.42659275275145 10-11 19.294295514769235 25.368621267049242 30.673191032789816 24.663892185391706 12-13 22.670004714450602 19.546274771186578 27.616926503340757 30.16679401102206 14-15 22.86996244696243 22.923609643489996 20.855754068245737 33.350673841301834 16-17 24.380212312844442 27.61367515809666 20.647667972623672 27.358444556435224 18-19 22.409084258612 26.50577926617138 21.254043860647343 29.831092614569275 20-21 21.000438931607952 24.051826443190873 21.320696438151284 33.62703818704989 22-23 23.325963617446718 29.948141043356692 21.42148814071822 25.30440719847837 24-25 24.272917919789315 31.69248776681352 21.41498545023003 22.619608863167134 26-27 21.79620568010014 36.29639263245168 21.754751028237933 20.15265065921025 28-29 17.82956448230455 30.773169899045733 29.333636792222784 22.063628826426935 30-31 20.75089818412368 27.285289288443092 29.59780859330548 22.366003934127747 32-33 18.969973826670785 22.529384032643506 34.89587566855786 23.604766472127842 34-35 22.31154390128916 24.921561295986212 33.91153089590818 18.855363906816443 36-37 18.134378098938438 27.07801602913205 32.98489750134118 21.80270837058833 38-39 20.34935704647798 23.256872531009705 28.96460910701803 27.429161315494287 40-41 19.190252466958206 24.254222684635767 31.159267146781982 25.396257701624048 42-43 24.866288426836604 22.679758750182888 32.64675759595533 19.807195227025183 44-45 19.095963454879456 25.447466389218537 29.73680360249053 25.71976655341147 46-47 24.968705802025585 21.00450311316307 27.742103295238408 26.284687789572935 48-49 21.85147854924975 20.58101539511973 29.144245931754263 28.423260123876254 50-51 17.06387267732024 21.078471217466227 33.69287792824281 28.164778176970724 52-53 18.68466828150147 21.184139937899303 38.077316989904574 22.05387479069465 54-55 17.151658998910797 21.024824020938663 34.22122153040821 27.602295449742332 56-57 22.856144229675028 21.23128444393868 30.030237510770082 25.88233381561621 58-59 17.710890380895094 27.993269715344727 33.26613886495537 21.029701038804806 60-61 19.51294848243461 36.2663176889438 24.20138832441923 20.019345504202363 62-63 15.201664688764977 42.0797229853852 22.17986441890332 20.5387479069465 64-65 14.94236990554842 43.101458228341976 21.167070375367807 20.789101490741793 66-67 14.759481735568091 42.484515468275 21.120738705639457 21.63526409051745 68-69 15.046412953359454 40.195568416432295 21.333701819127665 23.424316811080583 70-71 15.62352673418627 37.84159445970771 21.681595760245802 24.853283045860223 72-73 15.710500219465803 35.42096792547917 22.354624225773414 26.513907629281615 74-75 15.91045795197763 35.17711703217206 22.24326565116317 26.66915936468714 76 15.957602458017003 35.190935249459464 22.43915920211988 26.412303090403654 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 2.5 14 2.0 15 1.0 16 3.5 17 5.0 18 6.0 19 6.5 20 11.0 21 16.0 22 15.5 23 23.5 24 39.0 25 46.0 26 87.5 27 164.0 28 239.0 29 279.0 30 343.5 31 487.5 32 655.5 33 744.0 34 890.5 35 1125.5 36 1523.5 37 1833.0 38 2954.0 39 4997.0 40 5979.0 41 5651.5 42 5264.0 43 4846.5 44 4337.0 45 4126.5 46 4008.0 47 3928.5 48 3687.0 49 3322.5 50 3120.0 51 2857.5 52 2251.0 53 1774.5 54 1642.0 55 1478.0 56 1164.0 57 859.5 58 705.0 59 582.5 60 360.5 61 233.5 62 206.0 63 174.0 64 120.0 65 81.5 66 55.5 67 46.0 68 34.0 69 14.5 70 7.5 71 8.0 72 5.0 73 1.0 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 61513.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.70804545380651 #Duplication Level Percentage of deduplicated Percentage of total 1 62.867800997297394 26.8496090257344 2 17.528834075596667 14.972444849056297 3 7.852765406722241 10.061287857851186 4 4.19854592516463 7.1724676084730055 5 2.3828556202656923 5.088355307008275 6 1.6329793308210574 4.184481329149936 7 0.9287807848958928 2.7766488384569117 8 0.6737467169121846 2.301952432819079 9 0.4643903924479464 1.7849885390080145 >10 1.385558220090594 8.28280200933136 >50 0.038064786266225115 1.0892006567717394 >100 0.0342583076396026 2.983109261456928 >500 0.0 0.0 >1k 0.011419435879867533 12.45265228488287 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT 3331 5.415115504039796 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT 3225 5.242794206102775 No Hit CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 1104 1.794742574740299 No Hit CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 470 0.7640661323622648 No Hit TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT 287 0.46656804252759576 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 272 0.4421829531968852 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 181 0.29424674459057437 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 143 0.23247118495277422 No Hit CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 132 0.21458878611025312 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT 123 0.19995773251182675 No Hit CTTATAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 119 0.19345504202363728 No Hit CTTAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 108 0.17557264318111618 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 81 0.13167948238583713 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGT 77 0.12517679189764766 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGT 74 0.12029977403150553 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGTCTT 71 0.11542275616536343 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGTCTT 63 0.10241737518898444 No Hit CTTATTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 62 0.10079170256693708 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.026010761952757953 0.0 2 0.0 0.0 0.0 0.026010761952757953 0.0 3 0.0 0.0 0.0 0.029262107196852696 0.0 4 0.0 0.0 0.0 0.029262107196852696 0.0 5 0.0 0.0 0.0 0.03251345244094744 0.0 6 0.0 0.0 0.0 0.03251345244094744 0.0 7 0.0 0.0 0.0 0.03251345244094744 0.0 8 0.0 0.0 0.0 0.03251345244094744 0.0 9 0.0 0.0 0.0 0.03251345244094744 0.0 10 0.0 0.0 0.0 0.03251345244094744 0.0 11 0.0 0.0 0.0 0.03413912506299482 0.0 12 0.0 0.0 0.0 0.040641815551184304 0.0 13 0.0 0.0 0.0 0.04226748817323168 0.0 14 0.0 0.0 0.0 0.048770178661421165 0.0 15 0.0 0.0 0.0 0.048770178661421165 0.0 16 0.0 0.0 0.0 0.052021523905515905 0.0 17 0.0 0.0 0.0 0.05364719652756328 0.0 18 0.0 0.0 0.0 0.056898541771658026 0.0 19 0.0 0.0 0.0 0.06502690488189489 0.0 20 0.0 0.0 0.0 0.06665257750394225 0.0 21 0.0 0.0 0.0 0.069903922748037 0.0 22 0.0 0.0 0.0 0.069903922748037 0.0 23 0.0 0.0 0.0 0.07315526799213175 0.0 24 0.0 0.0 0.0 0.07315526799213175 0.0 25 0.0 0.0 0.0 0.07315526799213175 0.0 26 0.0 0.0 0.0 0.07803228585827386 0.0 27 0.0 0.0 0.0 0.09103766683465284 0.0 28 0.0 0.0 0.0 0.11867410140945817 0.0 29 0.0 0.0 0.0 0.1511875538504056 0.0 30 0.0 0.0 0.0 0.1804496610472583 0.0 31 0.0 0.0 0.0 0.2210914765984426 0.0 32 0.0 0.0 0.0 0.27311300050395854 0.0 33 0.0 0.0 0.0 0.34789394111813765 0.0 34 0.0 0.0 0.0 0.40316681026774825 0.0 35 0.0 0.0 0.0 0.5397233105197276 0.0 36 0.0 0.0 0.0 0.6909108643701332 0.0 37 0.0 0.0 0.0 0.9770292458504707 0.0 38 0.0 0.0 0.0 1.3655650025197925 0.0 39 0.0 0.0 0.0 1.8337587176694357 0.0 40 0.0 0.0 0.0 2.3263375221497893 0.0 41 0.0 0.0 0.0 2.9652268626144065 0.0 42 0.0 0.0 0.0 3.6496350364963503 0.0 43 0.0 0.0 0.0 4.373059353307431 0.0 44 0.0 0.0 0.0 5.029831092614569 0.0 45 0.0 0.0 0.0 5.7955228975988815 0.0 46 0.0 0.0 0.0 6.418155511843025 0.0 47 0.0 0.0 0.0 7.122071757189537 0.0 48 0.0 0.0 0.0 7.790223204851007 0.0 49 0.0 0.0 0.0 8.464877343000667 0.0 50 0.0 0.0 0.0 9.178547624079464 0.0 51 0.0 0.0 0.0 9.851576089607075 0.0 52 0.0 0.0 0.0 10.52297888251264 0.0 53 0.0 0.0 0.0 11.169996586087494 0.0 54 0.0 0.0 0.0 11.893420902898574 0.0 55 0.0 0.0 0.0 12.52093053500886 0.0 56 0.0 0.0 0.0 13.098044315835677 0.0 57 0.0 0.0 0.0 13.663778388308163 0.0 58 0.0 0.0 0.0 14.23438947864679 0.0 59 0.0 0.0 0.0 14.860273438135028 0.0 60 0.0 0.0 0.0 15.393494058166567 0.0 61 0.0 0.0 0.0 15.999869946190236 0.0 62 0.0 0.0 0.0 16.581860744883194 0.0 63 0.0 0.0 0.0 17.201242013883245 0.0 64 0.0 0.0 0.0 17.771853104221872 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGATT 15 0.0022148772 70.00001 21 AGCATAA 15 0.0022148772 70.00001 23 GTCCCAC 15 0.0022148772 70.00001 1 CCACCGT 15 0.0022148772 70.00001 9 CTTTACA 200 0.0 63.000004 1 TTTACAC 215 0.0 60.23256 2 CTATACA 105 0.0 59.999996 1 TTACACA 240 0.0 53.958336 3 GCTATAC 20 0.0069209477 52.5 3 ATATGTA 20 0.0069209477 52.5 31 AAGGAGT 20 0.0069209477 52.5 20 ACGACCA 20 0.0069209477 52.5 11 AGTCGCA 20 0.0069209477 52.5 24 CCATTGT 20 0.0069209477 52.5 14 GCATACC 20 0.0069209477 52.5 1 TTAATAC 30 5.835864E-4 46.666668 3 TCTTTAT 30 5.835864E-4 46.666668 1 ATAACAC 30 5.835864E-4 46.666668 4 CTTATAA 40 4.544027E-5 43.75 1 TAACACA 60 2.546658E-7 40.833336 5 >>END_MODULE