FastQCFastQC Report
Wed 25 May 2016
SRR1780495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732289
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTGGTC131101.7902767896281386No Hit
CTTTACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTGG26580.3629714497964601No Hit
CTATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTGG23430.31995564592667647No Hit
ATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTGGGC16980.23187566657426234No Hit
TATACACATCTGACGCTGCCGACGAAAATTTGCGTGTAGATCTCGGTGGT9880.13491941023284523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT16850.037.68502443
GGTGGTC15350.037.64337544
CTATACA4550.033.3498881
GATCATA502.3668163E-630.7882271
CTTTACA7200.030.2384381
TTTACAC7000.030.1248872
ATGATCG300.005761982429.31410630
AGGTACA300.005785114629.2900856
TGTACCA300.00578704529.2880842
CAGTAGT407.0451817E-427.4894891
TCGGTGG26550.027.31737342
TTACACA8100.026.5762253
GCGTGTA28400.026.08317431
CTCGGTG27550.026.00671841
TGCGTGT28600.025.90608430
AAATTTG29450.025.314625
GAAAATT29600.025.27270523
TTGCGTG29350.025.2440929
ATTTGCG29350.025.24063927
AATTTGC29450.025.22957626
Produced by FastQC (version 0.11.5)