FastQCFastQC Report
Wed 25 May 2016
SRR1780495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732289
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT489156.679739829493547No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT403645.512031452063325RNA PCR Primer, Index 43 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC123331.6841711400826722No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC61290.8369646410092191No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG48410.6610777985194369No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT40100.547598011167722RNA PCR Primer, Index 43 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC29930.40871841581670626No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC22400.3058901608517948No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG15450.21098227612322457No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG13030.17793521410262889No Hit
CTTAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC11950.16318693849013163No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC11580.15813428851177608No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG9200.12563345892127287No Hit
TTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC7870.10747123062069756No Hit
CTTATTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG7740.10569597522289698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA24100.042.7194728
CTTGAAA24050.042.5338727
CGTCTTC24350.042.37121618
TCTTCTG24000.042.2558420
CTGCTTG24100.041.7153824
CTTCTGC25050.041.3628321
GTCTTCT25350.040.5262119
TCTGCTT26000.039.76688823
TTCTGCT26100.039.53023522
GCTTGAA25550.039.43407426
TGAAAAA26350.039.2386729
TGCTTGA25750.038.87149425
GGTCATA357.228273E-637.758471
TAGCTCC357.2895364E-637.71206334
GAAAAAA27850.037.12528230
GGTCTAA601.9463187E-1036.7096251
TTTACAC14050.036.648422
CTTATAA1450.036.4564551
CTTTACA15200.033.763191
CTATACA8850.033.598641
Produced by FastQC (version 0.11.5)