FastQCFastQC Report
Wed 25 May 2016
SRR1780494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67226
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTG200.006927805552.49218841
ATGGAAT200.006927805552.49218822
CACTGGG200.006927805552.49218831
CCTAAGT200.006927805552.49218832
AAACGCT200.006927805552.4921886
AAGCATC200.006927805552.49218848
GATGGAA200.006927805552.49218821
GGATGGA350.001248265439.99404520
TGGATGG350.001248265439.99404519
TAGAGGA500.007168121627.9958325
TTTTTCC500.007168121627.99583226
ATGCCAG500.007168121627.99583211
AACGAAT750.001816788323.3298670
CGAACGA1501.4644609E-518.66388970
ACGAACG1901.11031884E-414.7346569
CCGACGA3655.329639E-1014.38142170
CGACGAA2559.563872E-512.35110370
GCCGACG4601.6787453E-811.411345569
TGCCGAC5158.841744E-810.19265868
CTGCCGA5301.4442649E-69.24390767
Produced by FastQC (version 0.11.5)