##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780494_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 67226 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.486894951358106 34.0 23.0 34.0 12.0 34.0 2 29.200175527325737 34.0 27.0 34.0 12.0 34.0 3 29.536905363996073 34.0 31.0 34.0 11.0 34.0 4 29.70490584000238 34.0 31.0 34.0 11.0 34.0 5 29.57096956534674 34.0 31.0 34.0 11.0 34.0 6 32.143843155921815 37.0 31.0 38.0 11.0 38.0 7 31.967229940796717 37.0 28.0 38.0 11.0 38.0 8 31.303245768006427 37.0 27.0 38.0 11.0 38.0 9 31.751033826198196 37.0 27.0 38.0 12.0 38.0 10-11 31.872698063249338 37.0 29.5 38.0 11.0 38.0 12-13 32.12895308362836 37.0 31.0 38.0 11.0 38.0 14-15 31.76602058727278 37.0 28.0 38.0 11.0 38.0 16-17 31.601374468211702 37.0 27.0 38.0 11.0 38.0 18-19 31.626401987326332 37.0 29.0 38.0 11.0 38.0 20-21 31.698316127688692 37.0 27.5 38.0 11.0 38.0 22-23 31.586082765596643 37.0 27.0 38.0 11.0 38.0 24-25 31.90539374646714 37.0 31.0 38.0 11.0 38.0 26-27 31.89702644810044 37.0 31.0 38.0 11.0 38.0 28-29 31.936282093237736 37.0 31.0 38.0 11.0 38.0 30-31 31.79532472555261 37.0 27.0 38.0 11.0 38.0 32-33 31.6324636301431 37.0 27.0 38.0 11.0 38.0 34-35 31.57476274060631 37.0 27.0 38.0 11.0 38.0 36-37 31.54343557552138 37.0 27.0 38.0 11.0 38.0 38-39 31.830638443459378 37.0 27.0 38.0 11.0 38.0 40-41 31.547860946657543 37.0 27.0 38.0 11.0 38.0 42-43 31.803290393597713 37.0 28.5 38.0 11.0 38.0 44-45 31.056280308214085 37.0 26.5 38.0 11.0 38.0 46-47 31.631645494302802 37.0 27.0 38.0 11.0 38.0 48-49 31.4734180227888 37.0 27.0 38.0 11.0 38.0 50-51 31.442856930354324 37.0 27.0 38.0 11.0 38.0 52-53 31.501985838812363 37.0 27.0 38.0 11.0 38.0 54-55 31.260368012376162 37.0 27.0 38.0 11.0 38.0 56-57 31.255436884538717 37.0 26.5 38.0 11.0 38.0 58-59 31.145159610864845 37.0 26.5 38.0 11.0 38.0 60-61 31.168409543926458 37.0 26.5 38.0 11.0 38.0 62-63 31.24268884062714 37.0 27.0 38.0 11.0 38.0 64-65 31.28135691547913 37.0 27.0 38.0 11.0 38.0 66-67 31.288125130157972 37.0 27.0 38.0 11.0 38.0 68-69 30.889930979085474 37.0 26.0 38.0 11.0 38.0 70-71 30.87910927319787 36.5 25.5 38.0 11.0 38.0 72-73 30.87150804748163 37.0 25.5 38.0 11.0 38.0 74-75 30.477679766756907 36.0 25.0 38.0 11.0 38.0 76 29.751316454943026 35.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.9126152671607954 1101 2 -0.6698677101672814 1101 3 0.27867629816338635 1101 4 -1.1372244727447267 1101 5 -0.5774602654475238 1101 6 -1.6880965754832573 1101 7 -1.0248078055156462 1101 8 0.6195525355407447 1101 9 -0.7786487088357994 1101 10-11 -0.7726185130331231 1101 12-13 -0.7047513482360479 1101 14-15 -0.04722978609893147 1101 16-17 -0.6286373485336973 1101 18-19 0.05243280105327486 1101 20-21 -0.0729742131018476 1101 22-23 0.32427315246326316 1101 24-25 -0.4164112849395494 1101 26-27 -0.6959705141994661 1101 28-29 -0.12822293696103415 1101 30-31 -0.9535995237082417 1101 32-33 0.1240748014822124 1101 34-35 0.6072028460542711 1101 36-37 0.481327938980634 1101 38-39 0.6587267050285561 1101 40-41 0.932298515769812 1101 42-43 -0.42687932484309954 1101 44-45 1.1381324026062885 1101 46-47 0.5724993691408038 1101 48-49 -0.4692592808090801 1101 50-51 -0.1546667709207732 1101 52-53 -0.28146352757811854 1101 54-55 -0.26764908214800087 1101 56-57 0.0589910907394291 1101 58-59 0.14520927542587714 1101 60-61 -0.0710351886661833 1101 62-63 -0.48306105351241513 1101 64-65 -0.375800627138581 1101 66-67 -0.12790295663799967 1101 68-69 0.2504450182628162 1101 70-71 0.9364328118973653 1101 72-73 -0.22631355596267966 1101 74-75 0.0462244342312097 1101 76 -1.1330960558547787 1102 1 -0.09963171251687086 1102 2 -0.5057740040245626 1102 3 -0.6769176418848346 1102 4 -0.31427294516186777 1102 5 -0.6756645909000412 1102 6 -1.0796184006658827 1102 7 -0.22593055192609413 1102 8 -0.032719946376559506 1102 9 -0.31792684047061925 1102 10-11 -0.4781134930611941 1102 12-13 -3.686758617299347 1102 14-15 -1.9488455333370496 1102 16-17 -2.9666847773724463 1102 18-19 -1.701966421660746 1102 20-21 -0.6458807588573627 1102 22-23 -1.495745559976914 1102 24-25 -0.6014665226235252 1102 26-27 -4.768247314372196 1102 28-29 -1.2318430744254982 1102 30-31 -1.5621136839874907 1102 32-33 -6.2237246875242285 1102 34-35 -1.384034693979558 1102 36-37 -1.464226057204904 1102 38-39 -0.43197465162335646 1102 40-41 -0.921726458140725 1102 42-43 -1.0484382150514584 1102 44-45 -5.104625523191693 1102 46-47 -1.4832421113010987 1102 48-49 -1.0019700656520065 1102 50-51 -6.545396915942188 1102 52-53 -2.3530745967061897 1102 54-55 -0.1277915845770181 1102 56-57 -2.1489509006960326 1102 58-59 -0.4594364477019788 1102 60-61 -0.6629780437527302 1102 62-63 -4.6348117462325575 1102 64-65 -1.2580741886197373 1102 66-67 -0.7645937335641229 1102 68-69 -6.201802273831177 1102 70-71 -1.0473197520667945 1102 72-73 -1.4473362627891184 1102 74-75 -6.721975213111268 1102 76 -2.3460940046834544 1103 1 -0.6213377515672498 1103 2 -0.6891410014778003 1103 3 0.3644160427262726 1103 4 0.19226960335837262 1103 5 0.2324469983391495 1103 6 -1.7748263863805853 1103 7 0.15924552605757114 1103 8 1.8764747493290663 1103 9 0.5663564065069302 1103 10-11 0.894372675376502 1103 12-13 -0.5740130887726664 1103 14-15 -0.2972545377572935 1103 16-17 -0.12398403676180791 1103 18-19 0.21434164894608543 1103 20-21 -0.23788626220913756 1103 22-23 0.1260453712019185 1103 24-25 0.7729224004173716 1103 26-27 -0.9401538744846043 1103 28-29 0.8706714889655025 1103 30-31 0.44861492465980035 1103 32-33 -3.5070594845149508 1103 34-35 -1.033254564592248 1103 36-37 -0.05936378736502945 1103 38-39 0.4970818748249286 1103 40-41 0.5121308645371805 1103 42-43 0.09110688023264046 1103 44-45 -5.60768456713069 1103 46-47 -0.24956920945396277 1103 48-49 0.031664781103067696 1103 50-51 -0.2053746683499007 1103 52-53 -0.10693133392113907 1103 54-55 -0.1124231820126873 1103 56-57 0.11158011420399916 1103 58-59 0.15484592108114015 1103 60-61 0.9637161396728189 1103 62-63 0.4337898754998264 1103 64-65 0.2506638459811228 1103 66-67 -0.5639116692217421 1103 68-69 -3.0893787499296543 1103 70-71 0.11817598869020784 1103 72-73 0.8767028461362649 1103 74-75 -1.3229382996719643 1103 76 -0.46605536412843307 1104 1 -1.4913950190883831 1104 2 0.15604579972209365 1104 3 0.5769330866313744 1104 4 0.13273865162671328 1104 5 -0.46498959833387943 1104 6 -0.23727525466528832 1104 7 0.9136769413834536 1104 8 1.2416779126778543 1104 9 0.08031877356447126 1104 10-11 0.1495403874026735 1104 12-13 -0.48235778470940005 1104 14-15 0.03136558768584763 1104 16-17 -0.3124623569145193 1104 18-19 0.8996838905604854 1104 20-21 0.33969759380395814 1104 22-23 -0.4578924526322794 1104 24-25 0.057691966820421214 1104 26-27 0.07095875164029408 1104 28-29 -1.083419048258385 1104 30-31 -0.9257919474427396 1104 32-33 -0.20921383411953443 1104 34-35 0.3229085114917467 1104 36-37 -0.03945650620877217 1104 38-39 -0.6268750381829769 1104 40-41 0.29128275981942764 1104 42-43 0.8683771283918951 1104 44-45 -2.7813278722847663 1104 46-47 -0.44712852224371247 1104 48-49 0.1754607996468316 1104 50-51 0.7821082877450003 1104 52-53 -0.4599583314668223 1104 54-55 -0.547015491802707 1104 56-57 0.2094939402198186 1104 58-59 0.6927788362706657 1104 60-61 -0.26893998249789774 1104 62-63 -0.2234231051382949 1104 64-65 0.27528888006893126 1104 66-67 -0.0678794903289166 1104 68-69 -3.4558908851900867 1104 70-71 -0.5210931686591245 1104 72-73 0.3465828570443641 1104 74-75 -1.0352371815917856 1104 76 -0.5049700082516964 1105 1 -1.0379128287640889 1105 2 0.34801815485234044 1105 3 -0.8071179500512429 1105 4 -0.4465276960606879 1105 5 0.3226233734896198 1105 6 0.06915749387272285 1105 7 0.14121548471146994 1105 8 0.5449208685523921 1105 9 0.39924487670110054 1105 10-11 -0.1147339347663845 1105 12-13 -1.843495477426039 1105 14-15 -0.07955945218656169 1105 16-17 -0.12641769981723172 1105 18-19 0.4557976598321467 1105 20-21 -0.35860118344219316 1105 22-23 -0.4291045738444019 1105 24-25 -0.46955417884677786 1105 26-27 -0.7020343371422655 1105 28-29 -1.339958123218512 1105 30-31 -0.35157610480584367 1105 32-33 0.1546351823610621 1105 34-35 0.19005364705793681 1105 36-37 -0.35046129089776556 1105 38-39 0.04718131482074739 1105 40-41 0.2769552744393131 1105 42-43 -0.3582310587426072 1105 44-45 0.7685126380820613 1105 46-47 0.48221757026027134 1105 48-49 -0.43652750444673316 1105 50-51 -0.026264917996627446 1105 52-53 -1.1222921964322623 1105 54-55 -0.691778915513499 1105 56-57 -0.22346725063610862 1105 58-59 -0.12635991971019678 1105 60-61 -0.09685066830331834 1105 62-63 0.24667258864160857 1105 64-65 0.10016926284404803 1105 66-67 0.15455538473753805 1105 68-69 0.28719789737238344 1105 70-71 0.7845155501074892 1105 72-73 0.4080395290273522 1105 74-75 -0.23340305081028845 1105 76 -1.1933804282410634 1106 1 0.04478919143793192 1106 2 -0.3653656835314969 1106 3 -0.8601482530815474 1106 4 -0.1870579990909924 1106 5 0.4375223633886094 1106 6 -0.34624654653131515 1106 7 0.08502861602459788 1106 8 0.7696683432998661 1106 9 0.8203307352869551 1106 10-11 0.3587509137184668 1106 12-13 0.2982343205537603 1106 14-15 -0.41289278551989383 1106 16-17 -0.01467527557480608 1106 18-19 -0.2228639563294692 1106 20-21 -0.09376532485633149 1106 22-23 0.271653001913176 1106 24-25 0.5395998615572637 1106 26-27 0.20074344063551308 1106 28-29 -0.8617384262488166 1106 30-31 -1.0349725694523073 1106 32-33 0.7250265965024774 1106 34-35 0.2733869803912725 1106 36-37 0.14101598182950426 1106 38-39 0.14692878956822142 1106 40-41 0.6184956784797144 1106 42-43 0.8365290422674967 1106 44-45 0.7789293047487291 1106 46-47 0.3325963581390674 1106 48-49 0.22193209151286553 1106 50-51 0.30612144563973587 1106 52-53 -0.14249421663428308 1106 54-55 0.012135225900639313 1106 56-57 0.6385908301719709 1106 58-59 -0.17181446516474352 1106 60-61 0.8254978165451661 1106 62-63 0.36314986136888194 1106 64-65 0.563237444662235 1106 66-67 1.2584063948385484 1106 68-69 0.39578375595824156 1106 70-71 0.1165862571781986 1106 72-73 0.6630900340778574 1106 74-75 -1.1472288083860462 1106 76 0.030735733375099272 1107 1 -0.40603568165851556 1107 2 -0.7252526240021915 1107 3 -0.0222387007049889 1107 4 -0.876692286123756 1107 5 -1.2412385964637203 1107 6 -0.2038261496692968 1107 7 -0.47251060871005635 1107 8 0.19364157818680638 1107 9 -1.4634429149207016 1107 10-11 -0.5403607614084365 1107 12-13 -0.5886628875680664 1107 14-15 -0.2029250882425515 1107 16-17 -0.02543323588860602 1107 18-19 0.31863927394279656 1107 20-21 -0.06185196537317594 1107 22-23 -1.3345421988251758 1107 24-25 -0.7210283110315672 1107 26-27 -0.18117857136264348 1107 28-29 -0.9311748591053011 1107 30-31 -0.8135691315196851 1107 32-33 0.4331051613386556 1107 34-35 0.2193952868056641 1107 36-37 -0.43098736380962777 1107 38-39 -0.27583309321904537 1107 40-41 0.5067917098595025 1107 42-43 0.19224835468096657 1107 44-45 0.016628312593674366 1107 46-47 0.26167457687464335 1107 48-49 0.8932807301838359 1107 50-51 0.8564069786346558 1107 52-53 0.68630135327804 1107 54-55 -0.046788401233644805 1107 56-57 0.5305064969924622 1107 58-59 0.8632570622925755 1107 60-61 -0.4824451922227162 1107 62-63 -0.2760546840856648 1107 64-65 -0.16425159548543888 1107 66-67 0.1873836675658218 1107 68-69 1.0519904933832542 1107 70-71 0.37933429594146517 1107 72-73 0.011036503851737933 1107 74-75 0.26986567707772835 1107 76 -0.7848791720240698 1108 1 -0.7819595132252815 1108 2 -0.13473685640007815 1108 3 -0.5164129728177684 1108 4 0.09900535465370908 1108 5 -0.17169807938105563 1108 6 0.0904585877126749 1108 7 0.6981056296845161 1108 8 1.0111897752358026 1108 9 -0.03467986292312375 1108 10-11 0.2648389966200213 1108 12-13 0.5814273257351061 1108 14-15 0.37632059177638055 1108 16-17 0.15321803577705495 1108 18-19 0.3361033069771757 1108 20-21 0.6066733232830934 1108 22-23 -0.50607898112969 1108 24-25 -0.35402060476398134 1108 26-27 -0.32098289002864533 1108 28-29 -0.3935772156977073 1108 30-31 -0.09801719969253497 1108 32-33 0.23646383154722272 1108 34-35 0.047056316236769646 1108 36-37 0.08312325926709718 1108 38-39 0.7250611494363284 1108 40-41 0.3696438179050574 1108 42-43 0.1455110253103591 1108 44-45 0.6746988525584285 1108 46-47 0.022451515934637456 1108 48-49 0.12233835623259992 1108 50-51 0.6702730000438812 1108 52-53 0.19099612721875303 1108 54-55 0.22954572990440525 1108 56-57 0.9095075753438984 1108 58-59 -0.9047395711468411 1108 60-61 0.6398819427816207 1108 62-63 -0.016987326572710515 1108 64-65 0.7610265402816339 1108 66-67 0.41018159502695895 1108 68-69 1.0194295402257865 1108 70-71 0.9549635534570484 1108 72-73 0.2678945559808241 1108 74-75 0.9672171407425445 1108 76 0.8603695063378858 1109 1 -0.36975162488860036 1109 2 -0.6206996352940202 1109 3 -0.4130239488143843 1109 4 -0.7959401883303237 1109 5 -1.0251399742737277 1109 6 -0.7142892181454172 1109 7 -0.873111458186905 1109 8 -0.8193854600396513 1109 9 -0.013505071763134424 1109 10-11 0.027118260657239546 1109 12-13 -0.8031687406707277 1109 14-15 -0.4984679246664605 1109 16-17 -0.2576019917158092 1109 18-19 -0.5163472587413445 1109 20-21 -0.6778910205891719 1109 22-23 0.24312795553098354 1109 24-25 -0.1612929955855904 1109 26-27 -0.6397018283811882 1109 28-29 0.1629021117845788 1109 30-31 -0.2598682095265197 1109 32-33 -0.5406853701023486 1109 34-35 -0.06473917916346039 1109 36-37 0.10756282784063842 1109 38-39 -0.08173540338168905 1109 40-41 -0.2814347482364248 1109 42-43 -0.1853285644115381 1109 44-45 -0.06690789376704487 1109 46-47 -0.9100984470557414 1109 48-49 -0.24038237973018184 1109 50-51 0.1894122619662646 1109 52-53 0.07457442900579281 1109 54-55 0.2782530366613081 1109 56-57 -0.6797649212194976 1109 58-59 -0.2875380273168773 1109 60-61 0.02088278871585203 1109 62-63 -0.2888097861264818 1109 64-65 -0.1354522584917568 1109 66-67 0.041975504300516775 1109 68-69 0.8220360750677003 1109 70-71 -0.4355937057160517 1109 72-73 0.11508446821514795 1109 74-75 1.0180657185156257 1109 76 -0.09159729074363909 1110 1 0.2391952687859984 1110 2 -0.10714117323516348 1110 3 -0.7819212314843575 1110 4 0.5887142259215636 1110 5 0.17691211728112322 1110 6 0.18947670811459005 1110 7 0.08392992190104209 1110 8 1.0452836120090545 1110 9 0.41503816873748534 1110 10-11 -0.572878920535679 1110 12-13 -0.1201013148053569 1110 14-15 0.038387977915565585 1110 16-17 0.01279207751409217 1110 18-19 0.23566818832145842 1110 20-21 -0.09167780602157549 1110 22-23 0.024980475544516878 1110 24-25 0.4023415742644474 1110 26-27 -1.2968080410319942 1110 28-29 0.11040182990387137 1110 30-31 0.09475181527898258 1110 32-33 0.8595852353774127 1110 34-35 0.006435699627832747 1110 36-37 -0.2685928830625066 1110 38-39 0.44068820694641886 1110 40-41 -0.3212644922887442 1110 42-43 0.05644051891239954 1110 44-45 0.478122549349429 1110 46-47 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32-33 0.20540604892140024 2116 34-35 -0.9791382721339801 2116 36-37 0.7880288739081891 2116 38-39 0.4558734998287193 2116 40-41 0.5693529352580278 2116 42-43 0.5628215728363379 2116 44-45 1.1324148177737143 2116 46-47 0.43795919705453557 2116 48-49 0.43920348970531364 2116 50-51 0.7569620889145945 2116 52-53 1.1608019024513112 2116 54-55 0.5228675491329646 2116 56-57 0.843119671213973 2116 58-59 0.018509830422711815 2116 60-61 -0.6405774093984817 2116 62-63 0.8964599357973739 2116 64-65 1.07411928942566 2116 66-67 0.5060971178582179 2116 68-69 0.7078518048683975 2116 70-71 -2.160859250530468 2116 72-73 0.6381033248806212 2116 74-75 -0.8568746084923724 2116 76 -0.19444369246222237 2117 1 0.071246246652656 2117 2 -0.09493902430115497 2117 3 -0.0909067583799299 2117 4 0.5412953193696879 2117 5 -1.10577099969003 2117 6 -0.22668436025134042 2117 7 -1.690940430266533 2117 8 -0.25919389875256016 2117 9 1.1769606850917533 2117 10-11 0.1570637971540485 2117 12-13 0.27963769478688505 2117 14-15 0.6958634888805513 2117 16-17 1.1238146853844775 2117 18-19 0.5526867965985822 2117 20-21 0.7216801575753458 2117 22-23 1.024929633257294 2117 24-25 0.6414020087965326 2117 26-27 0.44091354660316284 2117 28-29 0.7033584783802986 2117 30-31 0.37270659953821195 2117 32-33 1.3850063789899245 2117 34-35 0.30545613822729933 2117 36-37 -0.14326664505281528 2117 38-39 0.06752276614379227 2117 40-41 -0.9241353867740507 2117 42-43 -0.28120311054901137 2117 44-45 0.665798378926084 2117 46-47 0.6071013776594221 2117 48-49 -0.20629573893889486 2117 50-51 0.9708070284618238 2117 52-53 1.3148398603316949 2117 54-55 -0.1843580814055521 2117 56-57 -0.2562955334643924 2117 58-59 -0.4827458650531966 2117 60-61 0.469565022345499 2117 62-63 -0.6288154202819811 2117 64-65 0.06224399792492008 2117 66-67 -0.5610825901028882 2117 68-69 0.18133869181992424 2117 70-71 0.6144250748022948 2117 72-73 0.9458563475190154 2117 74-75 -0.37120747542453003 2117 76 -0.49021379255908926 2118 1 1.0480034771522178 2118 2 0.4623615891957762 2118 3 1.2288127858794944 2118 4 0.2044679749349818 2118 5 0.9348600257262696 2118 6 1.486935271650502 2118 7 1.542398745894726 2118 8 -3.1844875008559796 2118 9 -1.4284030309468072 2118 10-11 0.1451794851470396 2118 12-13 1.1259128919823311 2118 14-15 -0.17081486344197216 2118 16-17 0.4196429062433751 2118 18-19 -0.39257174853726085 2118 20-21 -1.5953400001810074 2118 22-23 0.039250404510575265 2118 24-25 0.7794212901286954 2118 26-27 1.35509408998616 2118 28-29 -0.07311829637868428 2118 30-31 -0.06180698503672488 2118 32-33 0.41604932377520143 2118 34-35 1.1554649024691912 2118 36-37 0.8131750727385949 2118 38-39 0.3124482700876996 2118 40-41 -1.1322510747670371 2118 42-43 0.23161021109866553 2118 44-45 0.8554390450084668 2118 46-47 0.3520444100871174 2118 48-49 0.19982170190247572 2118 50-51 1.1445143027825928 2118 52-53 -0.815221489361555 2118 54-55 0.9908040570694716 2118 56-57 1.6922758951070342 2118 58-59 1.650523197172923 2118 60-61 -0.9347294561821045 2118 62-63 0.5210881730571941 2118 64-65 0.31107835375313897 2118 66-67 -0.5869020467198958 2118 68-69 1.3087707689452515 2118 70-71 0.202365477957418 2118 72-73 -0.5595073685195473 2118 74-75 0.9369432695360302 2118 76 0.36044352558289106 2119 1 1.0835550142227426 2119 2 1.0735008297021054 2119 3 0.6855939793695462 2119 4 1.3148838881357072 2119 5 1.5996339859432673 2119 6 1.2013656513973388 2119 7 -1.1762630985898994 2119 8 1.2152955009523403 2119 9 0.6462081806264273 2119 10-11 1.160966103591889 2119 12-13 1.4006868521993283 2119 14-15 0.48296452172981574 2119 16-17 0.5967315138383107 2119 18-19 0.1089110724392306 2119 20-21 -0.2485768898735934 2119 22-23 1.7237531169879716 2119 24-25 1.5613561906711908 2119 26-27 1.287481070094664 2119 28-29 1.6740426439468088 2119 30-31 1.0154805375672566 2119 32-33 1.3371885642815293 2119 34-35 1.4920290977675634 2119 36-37 0.8043143132449302 2119 38-39 0.6557032429629288 2119 40-41 0.9991774966615345 2119 42-43 1.0042865221294086 2119 44-45 -0.05964232931341229 2119 46-47 0.954503722926173 2119 48-49 0.6766933113057298 2119 50-51 1.764586635513158 2119 52-53 0.527816485321992 2119 54-55 0.504583442241259 2119 56-57 1.8861095298267436 2119 58-59 2.1089680434658646 2119 60-61 1.8589414298938394 2119 62-63 0.5720465817371192 2119 64-65 2.0328505056518793 2119 66-67 1.310801389084812 2119 68-69 0.9869805338638784 2119 70-71 1.1431973043588677 2119 72-73 0.0037114379904004124 2119 74-75 1.9137967957566495 2119 76 1.5280746286570306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 71.0 13 321.0 14 770.0 15 1241.0 16 1578.0 17 1854.0 18 2017.0 19 1893.0 20 1752.0 21 1354.0 22 1065.0 23 860.0 24 760.0 25 722.0 26 817.0 27 846.0 28 1030.0 29 1211.0 30 1469.0 31 1794.0 32 2316.0 33 3073.0 34 4351.0 35 6569.0 36 11580.0 37 15907.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.50412752633078 27.685139407014546 10.289601030757936 28.521132035896745 2 17.17044000833011 24.085919138428586 34.7514354565198 23.992205396721506 3 15.798946836045577 30.28441376848243 28.123047630381105 25.79359176509089 4 11.837857939754555 21.637783562662698 37.14391967274079 29.38043882484195 5 13.505191443786629 35.239341921280456 33.725046856870854 17.530419778062058 6 25.101895100110077 37.28319400232053 18.73977330199625 18.87513759557314 7 22.41097194537828 35.065302115253026 20.625948293814893 21.897777645553802 8 19.837562847707733 38.309582601969474 19.383869336268706 22.468985214054086 9 20.301668997114213 23.301996251450333 20.152917026150597 36.24341772528486 10-11 21.310207360247524 28.205604974265906 26.032338678487488 24.451848986999078 12-13 21.003778300062475 25.8597863921697 25.716984500044624 27.4194508077232 14-15 19.97664594055871 26.87353107428673 22.987385832862287 30.162437152292267 16-17 20.70106803915152 28.94415851010026 24.425817392080447 25.92895605866778 18-19 19.464939160443876 28.259155683812807 25.15990836878589 27.115996786957425 20-21 19.353375182221164 28.32386279118198 24.862404426858657 27.460357599738195 22-23 21.313182399666797 27.89173831553268 24.74117157052331 26.053907714277212 24-25 20.17151102252105 29.32793859518639 24.50019337756225 26.00035700473031 26-27 19.123553387082378 29.78683842560914 25.775741528575253 25.313866658733232 28-29 20.218367893374587 28.534346830095497 27.03938952191117 24.20789575461875 30-31 20.156635825424686 28.30675631452117 27.028976883943713 24.507630976110434 32-33 18.856543599202688 29.73328771606224 26.289679588254543 25.12048909648053 34-35 19.71409871180793 28.497158837354597 26.556689376134234 25.23205307470324 36-37 19.949870585785263 27.856037842501415 26.771635974176654 25.42245559753667 38-39 19.528902507958232 27.422425847142474 27.80025585339006 25.248415791509238 40-41 19.28941183470681 26.792461250111565 27.671585398506533 26.246541516675098 42-43 17.99750096688781 26.727754142742395 28.476333561419686 26.79841132895011 44-45 18.280873471573496 26.454794276024156 28.812513015797457 26.451819236604884 46-47 18.36566209502276 26.957575937881177 28.486002439532328 26.19075952756374 48-49 17.78850444768393 27.989914616368665 27.761580340939517 26.460000595007884 50-51 17.756522773926754 27.720673548924523 27.334662184273938 27.188141492874777 52-53 16.819385356855978 27.91107607175795 27.48415791509238 27.785380656293697 54-55 16.85359831017761 28.157260583702737 26.91443786630173 28.07470323981793 56-57 17.34522357421236 27.780174337309973 26.933775622527 27.940826465950675 58-59 17.254613204810674 27.2976772206975 27.134048835635287 28.313660738856534 60-61 17.041769553446585 26.86163091660965 27.158391098682056 28.938208431261714 62-63 17.473894029095884 27.223098206051233 26.78204861214411 28.520959152708773 64-65 18.097908547288252 27.711748430666706 25.148008211108795 29.042334810936243 66-67 17.369023889566538 29.139023592062596 24.251777586053016 29.240174932317853 68-69 17.526700978787968 28.75524350697647 24.333591170083004 29.384464344152562 70-71 17.88519322881028 29.507184720197543 24.001874274834144 28.605747776158037 72-73 18.248148037961503 29.622467497694345 23.43810430488204 28.691280159462114 74-75 18.39987504834439 29.564454229018533 23.78544015708208 28.250230565554997 76 18.723410585190255 28.389313658405975 24.52027489364234 28.367000862761433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 1.5 10 2.0 11 1.0 12 1.0 13 2.0 14 3.5 15 8.5 16 11.5 17 10.0 18 18.0 19 25.0 20 36.5 21 49.0 22 54.5 23 77.0 24 104.5 25 115.0 26 162.0 27 240.0 28 351.0 29 431.0 30 506.0 31 667.0 32 920.5 33 1088.0 34 1240.5 35 1523.5 36 1888.5 37 2123.0 38 2391.0 39 2954.5 40 3565.5 41 3995.0 42 4109.0 43 4269.0 44 4680.0 45 4883.5 46 4836.0 47 4887.5 48 4782.5 49 4375.0 50 4124.0 51 3828.0 52 3208.5 53 2644.0 54 2403.0 55 2236.5 56 1843.0 57 1362.0 58 1108.0 59 975.0 60 722.5 61 528.0 62 453.0 63 380.5 64 247.5 65 165.0 66 117.5 67 92.0 68 80.0 69 52.5 70 36.0 71 35.0 72 28.5 73 20.5 74 18.0 75 17.0 76 13.5 77 10.0 78 9.0 79 8.0 80 8.0 81 6.5 82 6.0 83 7.0 84 7.5 85 7.0 86 6.0 87 6.0 88 3.5 89 2.0 90 2.0 91 2.0 92 3.0 93 3.0 94 3.0 95 3.0 96 3.0 97 2.0 98 2.0 99 4.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7125219409157171 2 0.0 3 0.0 4 0.0014875197096361527 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.437598548180763E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 67226.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.4284949275578 #Duplication Level Percentage of deduplicated Percentage of total 1 80.86300724171916 56.14196888108768 2 9.784890945708531 13.587005027816618 3 4.2293353901529755 8.809091720465297 4 1.9325534558855038 5.366971112367239 5 1.084115353301624 3.763424865379466 6 0.674893945237177 2.8114122512123285 7 0.4327891331362214 2.1033528694255197 8 0.27424261901701164 1.5232201826674203 9 0.1949693619574067 1.218278642192009 >10 0.5292025538843896 4.675274447386428 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.005950078838544611 0.0 6 0.0 0.0 0.0 0.005950078838544611 0.0 7 0.0 0.0 0.0 0.005950078838544611 0.0 8 0.0 0.0 0.0 0.007437598548180763 0.0 9 0.0 0.0 0.0 0.008925118257816916 0.0 10 0.0 0.0 0.0 0.010412637967453068 0.0 11 0.0 0.0 0.0 0.011900157677089221 0.0 12 0.0 0.0 0.0 0.011900157677089221 0.0 13 0.0 0.0 0.0 0.013387677386725375 0.0 14 0.0 0.0 0.0 0.014875197096361526 0.0 15 0.0 0.0 0.0 0.014875197096361526 0.0 16 0.0 0.0 0.0 0.017850236515633833 0.0 17 0.0 0.0 0.0 0.02231279564454229 0.0 18 0.0 0.0 0.0 0.025287835063814594 0.0 19 0.0 0.0 0.0 0.037187992740903814 0.0 20 0.0 0.0 0.0 0.037187992740903814 0.0 21 0.0 0.0 0.0 0.03867551245053997 0.0 22 0.0 0.0 0.0 0.04313807157944843 0.0 23 0.0 0.0 0.0 0.04611311099872073 0.0 24 0.0 0.0 0.0 0.04908815041799304 0.0 25 0.0 0.0 0.0 0.05057567012762919 0.0 26 0.0 0.0 0.0 0.06396334751435456 0.0 27 0.0 0.0 0.0 0.08032606432035225 0.0 28 0.0 0.0 0.0 0.09371374170707762 0.0 29 0.0 0.0 0.0 0.11453901764198375 0.0 30 0.0 0.0 0.0 0.15023949067325143 0.0 31 0.0 0.0 0.0 0.1859399637045191 0.0 32 0.0 0.0 0.0 0.2394906732514206 0.0 33 0.0 0.0 0.0 0.2900663433790498 0.0 34 0.0 0.0 0.0 0.3480796120548597 0.0 35 0.0 0.0 0.0 0.41055543985957815 0.0 36 0.0 0.0 0.0 0.5682325290810103 0.0 37 0.0 0.0 0.0 0.798798084074614 0.0 38 0.0 0.0 0.0 1.091839466872936 0.0 39 0.0 0.0 0.0 1.451819236604885 0.0 40 0.0 0.0 0.0 1.9546008984619045 0.0 41 0.0 0.0 0.0 2.5079582304465533 0.0 42 0.0 0.0 0.0 3.1728795406539136 0.0 43 0.0 0.0 0.0 4.019278255436885 0.0 44 0.0 0.0 0.0 4.740725314610419 0.0 45 0.0 0.0 0.0 5.386308868592509 0.0 46 0.0 0.0 0.0 6.09734328979859 0.0 47 0.0 0.0 0.0 6.68491357510487 0.0 48 0.0 0.0 0.0 7.202570434058251 0.0 49 0.0 0.0 0.0 7.738077529527266 0.0 50 0.0 0.0 0.0 8.24680927022283 0.0 51 0.0 0.0 0.0 8.8462797132062 0.0 52 0.0 0.0 0.0 9.532026299348466 0.0 53 0.0 0.0 0.0 10.385862612679619 0.0 54 0.0 0.0 0.0 11.16234790110969 0.0 55 0.0 0.0 0.0 11.82726921131705 0.0 56 0.0 0.0 0.0 12.466902686460596 0.0 57 0.0 0.0 0.0 12.971171868027252 0.0 58 0.0 0.0 0.0 13.453128253949364 0.0 59 0.0 0.0 0.0 13.893434088001666 0.0 60 0.0 0.0 0.0 14.406628387826139 0.0 61 0.0 0.0 0.0 14.857346859845894 0.0 62 0.0 0.0 0.0 15.352690923154732 0.0 63 0.0 0.0 0.0 15.885222979204475 0.0 64 0.0 0.0 0.0 16.456430547704755 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCTG 20 0.0069278055 52.492188 41 ATGGAAT 20 0.0069278055 52.492188 22 CACTGGG 20 0.0069278055 52.492188 31 CCTAAGT 20 0.0069278055 52.492188 32 AAACGCT 20 0.0069278055 52.492188 6 AAGCATC 20 0.0069278055 52.492188 48 GATGGAA 20 0.0069278055 52.492188 21 GGATGGA 35 0.0012482654 39.994045 20 TGGATGG 35 0.0012482654 39.994045 19 TAGAGGA 50 0.0071681216 27.995832 5 TTTTTCC 50 0.0071681216 27.995832 26 ATGCCAG 50 0.0071681216 27.995832 11 AACGAAT 75 0.0018167883 23.32986 70 CGAACGA 150 1.4644609E-5 18.663889 70 ACGAACG 190 1.11031884E-4 14.73465 69 CCGACGA 365 5.329639E-10 14.381421 70 CGACGAA 255 9.563872E-5 12.351103 70 GCCGACG 460 1.6787453E-8 11.4113455 69 TGCCGAC 515 8.841744E-8 10.192658 68 CTGCCGA 530 1.4442649E-6 9.243907 67 >>END_MODULE