FastQCFastQC Report
Wed 25 May 2016
SRR1780494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67226
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT31294.654449171451522No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT29234.348020111266474Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC10091.5009073870228782No Hit
CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC5260.7824353672686163No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG3080.458156070567935No Hit
TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT2440.36295480915122125No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1550.23056555499360365No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1320.19635260167197216No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1280.19040252283342754No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT1210.17998988486597448No Hit
CTTAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC1190.17701484544670218No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG900.13387677386725375No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG840.12495165560943683No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGT830.12346413589980068No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC810.12048909648052837No Hit
TTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC680.10115134025525839No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAGT150.002215774770.0000119
GACACAT150.002215774770.0000126
CCCAGTG150.002215774770.0000120
CGTCTGC150.002215774770.0000138
TCCGTAC150.002215774770.0000118
ACATCAG150.002215774770.0000122
GTGACAC150.002215774770.0000124
GAGGCAC150.002215774770.000017
TGCCCAG207.874507E-570.018
CTTTACA1850.058.6486471
CTATACA1000.056.0000041
TTTACAC2050.052.926832
ATCCTCT200.006923730552.56
ATTAGAG200.006923730552.53
CCGATAG200.006923730552.530
GCTATAC200.006923730552.53
CCCAGTT200.006923730552.527
GCTGACC200.006923730552.545
GGCCCGA200.006923730552.527
GCACCGC200.006923730552.510
Produced by FastQC (version 0.11.5)