FastQCFastQC Report
Wed 25 May 2016
SRR1780493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553857
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTCC250.002358267835.17997729
ACGAATC353.2008073E-431.45332524
CGGTATG353.236645E-431.39367543
CGAACGC300.005763433429.3113532
ACGGACC406.94704E-427.5540721
ACCTCGA407.017235E-427.50672539
ACGACCT407.017235E-427.50672536
TTTACGA407.058141E-427.47939333
GATCATA508.3503066E-526.3921361
GTTTACG450.001403373224.42612332
CTTAACT450.001405590824.41950642
CGACAAT551.5719852E-424.0450117
AATGCTC852.362849E-723.27876528
TAAGACT852.3794018E-723.261959
CGGTGGT1254.0017767E-1122.85459343
CCAGATT602.8339037E-422.0412618
GACGAAT703.198522E-522.01135323
TAACGGC703.2052296E-522.00538336
CGAATCT602.870568E-421.99941325
GTACATG1205.984475E-1021.9934481
Produced by FastQC (version 0.11.5)