FastQCFastQC Report
Wed 25 May 2016
SRR1780493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553857
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT332516.0035352085466105No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT260834.70933833101324RNA PCR Primer, Index 24 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC85031.5352338238931709No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC44530.803998143925237No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG39800.7185970385857722No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT22140.3997421717158039No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC16160.29177206390819294No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC15860.2863555033158378No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG12040.21738463177318332No Hit
CTTATAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG9760.17621877127128482No Hit
CTTAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC9690.17495490713306863No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC8910.16087184959294548No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC7240.13071966229550228No Hit
CTTATTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG6190.11176170022225954No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG5660.10219244317576559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG207.820854E-444.0365941
ACGGATA207.855722E-443.9968443
CCGTCTT27050.042.5329944
GCCGTCT30100.038.2231743
CTATACA5650.037.411621
ATCCCGG301.2987026E-436.673964
CAGCGAC250.002352524835.1974733
GTCGACG250.002352524835.1974731
CGACCAG250.002352524835.1974736
AGCGACC250.002352524835.1974734
CTTTACA9950.035.185021
TTTACAC10450.033.269782
CTTTATA2450.031.4348262
TTTCGAC353.216983E-431.42631328
ATTTCGA353.216983E-431.42631327
TTACACA11200.031.2383613
CTTATAC48950.029.957481
TTATACA49050.029.7877832
TCTTTAT2550.029.357731
GAGTCTT300.00574437729.33122639
Produced by FastQC (version 0.11.5)