FastQCFastQC Report
Wed 25 May 2016
SRR1780492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13852
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGGTC160.11550678602367889No Hit
CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG150.10828761189719896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATACC200.006808627452.558
CCCATAC450.004164525331.11110957
ACTACTC500.006970922527.9999988
TTATACA750.001743623923.3333342
CTTATAC952.594168E-422.1052651
TATACAC850.003607681720.5882363
Produced by FastQC (version 0.11.5)