##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780492_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13852 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.023678891134853 12.0 12.0 23.0 12.0 32.0 2 18.235489460005777 12.0 11.0 23.0 11.0 32.0 3 19.007868899797863 21.0 11.0 23.0 11.0 32.0 4 20.11413514293965 21.0 11.0 31.0 11.0 33.0 5 20.289055732024256 21.0 11.0 31.0 11.0 33.0 6 19.529959572624893 11.0 11.0 27.0 11.0 34.0 7 21.863485417268265 21.0 11.0 31.0 11.0 35.0 8 20.17853017614785 21.0 11.0 28.0 11.0 34.0 9 21.13312157089229 21.0 12.0 31.0 10.0 36.0 10-11 20.51216430840312 21.0 11.0 29.5 10.5 34.5 12-13 20.517145538550388 21.0 11.0 29.5 11.0 34.0 14-15 20.30194195784002 21.0 11.0 29.5 11.0 34.0 16-17 19.80970257002599 16.5 11.0 27.5 11.0 34.0 18-19 20.302736066993937 21.0 11.0 28.0 11.0 34.0 20-21 20.36435171816344 21.0 11.0 29.0 11.0 34.0 22-23 19.541582442968526 16.0 11.0 27.0 10.5 34.0 24-25 19.705277216286454 16.0 11.0 27.0 10.5 34.0 26-27 19.359262200404274 11.5 11.0 26.5 10.5 34.0 28-29 18.973866589662144 11.0 11.0 26.0 11.0 34.0 30-31 18.934630378284723 11.0 11.0 26.0 11.0 34.0 32-33 19.371570892289924 16.0 11.0 26.5 11.0 34.0 34-35 19.5875685821542 16.0 11.0 26.5 11.0 34.0 36-37 19.363196650303205 11.0 11.0 26.0 11.0 34.0 38-39 18.65568149003754 11.0 11.0 26.0 11.0 34.0 40-41 19.103595148714987 11.0 11.0 26.0 11.0 34.0 42-43 19.204771874097602 16.0 11.0 26.5 11.0 34.0 44-45 18.945206468380018 11.0 11.0 26.0 11.0 34.0 46-47 19.137128212532488 11.0 11.0 26.0 11.0 34.0 48-49 19.46639474444124 16.0 11.0 26.5 11.0 34.0 50-51 19.50866300895178 15.5 11.0 26.5 11.0 34.0 52-53 19.517109442679757 20.5 11.0 26.0 11.0 34.0 54-55 19.53335258446434 21.0 11.0 26.0 11.0 34.0 56-57 18.83139618827606 15.0 11.0 26.0 11.0 33.5 58-59 18.94080277216286 16.0 11.0 26.0 11.0 33.5 60-61 19.28836991048224 19.0 11.0 26.0 11.0 33.0 62-63 18.72935316199827 15.0 11.0 25.5 11.0 33.0 64-65 18.98566993935894 15.0 11.0 26.0 11.0 33.0 66-67 19.587063239965346 19.0 11.0 26.0 11.0 33.5 68-69 19.212027144094716 19.0 11.0 26.0 11.0 33.0 70-71 19.697263933006063 19.0 11.0 26.5 11.0 33.5 72-73 18.830602079122148 15.0 11.0 25.0 11.0 33.0 74-75 18.432825584753104 11.0 11.0 25.0 11.0 32.5 76 17.411492925209355 11.0 11.0 24.0 11.0 31.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -1.112301426192051 1101 2 1.5201223295025734 1101 3 1.3520648772900827 1101 4 2.158302134599108 1101 5 0.21540166913576542 1101 6 2.367313599401566 1101 7 -1.4491716873047693 1101 8 -0.3220529996728736 1101 9 -0.3330059781723236 1101 10-11 1.22819943282542 1101 12-13 2.2569028299428524 1101 14-15 0.9101454250447105 1101 16-17 1.7227523056785081 1101 18-19 1.399018769813818 1101 20-21 4.103592377201274 1101 22-23 2.0470600274339965 1101 24-25 0.5127269585441141 1101 26-27 0.8396584981304684 1101 28-29 1.2774124302327685 1101 30-31 -0.8924738386562083 1101 32-33 -0.9711335577731219 1101 34-35 1.90442316170558 1101 36-37 0.956232654585623 1101 38-39 1.4735041135648146 1101 40-41 0.704750904027474 1101 42-43 -0.3123760452464346 1101 44-45 0.45281679960308097 1101 46-47 0.8456211637113249 1101 48-49 1.3928464796057973 1101 50-51 -2.1339198396221235 1101 52-53 -0.7552994378148767 1101 54-55 -0.32581650320974376 1101 56-57 0.2576907753230948 1101 58-59 0.5914482072136167 1101 60-61 -0.8646950477863875 1101 62-63 0.5791539044488516 1101 64-65 -0.0035144348283644433 1101 66-67 -0.4599308312486947 1101 68-69 0.8681712192995299 1101 70-71 -0.6486984861806775 1101 72-73 1.6547051956384635 1101 74-75 2.1197495414251293 1101 76 1.0216804305912923 1102 1 -0.4745134077588702 1102 2 -0.17849518201816394 1102 3 0.7631247851241838 1102 4 2.6974726415115526 1102 5 2.589595217522863 1102 6 -1.9709352485247038 1102 7 -1.2740564799314988 1102 8 -2.303619819949372 1102 9 -3.5569691118128794 1102 10-11 -0.3381600141791914 1102 12-13 -1.1781202115317981 1102 14-15 -0.9695780772594347 1102 16-17 -2.3136532242754093 1102 18-19 0.33081600483685847 1102 20-21 -2.882121908513014 1102 22-23 -1.5474100186489537 1102 24-25 -0.28358640551118697 1102 26-27 -0.7695580917312803 1102 28-29 -1.4419424084769084 1102 30-31 -3.430261857089386 1102 32-33 -2.2623777974044543 1102 34-35 -2.3218441194004136 1102 36-37 -0.9714171149996282 1102 38-39 -2.421887591504312 1102 40-41 -1.7261246720093908 1102 42-43 -1.2524682111450538 1102 44-45 0.8740149562851123 1102 46-47 -1.9714295275329157 1102 48-49 0.037546940435291276 1102 50-51 -0.45465716681106016 1102 52-53 -0.24654367744621197 1102 54-55 -1.9087658119655053 1102 56-57 -1.4082078421884283 1102 58-59 -2.9670771384085057 1102 60-61 -1.7172296099983697 1102 62-63 -0.22729770845437258 1102 64-65 -0.8998277988836669 1102 66-67 -1.8322810616634406 1102 68-69 -1.0147780894562324 1102 70-71 -1.236256089867311 1102 72-73 -2.3853869702601536 1102 74-75 -1.6641214263168074 1102 76 -3.215185928855714 1103 1 -0.1414123624627024 1103 2 -0.19034187191363472 1103 3 0.6195707781555448 1103 4 0.07726358227810337 1103 5 -1.9616242946722622 1103 6 0.43812398492477556 1103 7 0.6562571089186768 1103 8 -1.7217382868483284 1103 9 -1.1646346170393578 1103 10-11 -1.1573237772453915 1103 12-13 -1.8788170756432976 1103 14-15 -1.0709718054824329 1103 16-17 -0.25128388629631715 1103 18-19 0.14370799089957842 1103 20-21 -2.725675915481652 1103 22-23 -2.270406534328398 1103 24-25 -0.19891741596414647 1103 26-27 0.13706211732795026 1103 28-29 0.1263502744499192 1103 30-31 -2.2901921706782353 1103 32-33 -0.8243987033278017 1103 34-35 0.7934868910525488 1103 36-37 -0.9564345366024192 1103 38-39 -0.2117830618875871 1103 40-41 -0.855044532636569 1103 42-43 -0.4406215212495823 1103 44-45 -1.2458456708925887 1103 46-47 0.4372812738608154 1103 48-49 0.6284877069858119 1103 50-51 0.4742626938161152 1103 52-53 -0.15734507117443286 1103 54-55 1.7166697281041827 1103 56-57 -1.4256294449758862 1103 58-59 -0.6980875913701787 1103 60-61 -2.5255919793363475 1103 62-63 -0.04437087918607929 1103 64-65 -1.6419880776293105 1103 66-67 -2.815556322987483 1103 68-69 -2.345440110362155 1103 70-71 0.5180993108295517 1103 72-73 -0.5589061340232213 1103 74-75 0.6724639395368506 1103 76 -0.22772948286268146 1104 1 -0.9316562649017257 1104 2 -1.8927808963038792 1104 3 0.8488390708384692 1104 4 0.5510440700829804 1104 5 -1.2104047824771378 1104 6 0.2076361800467268 1104 7 2.408086377211358 1104 8 -0.3857626770922309 1104 9 1.3823166024728373 1104 10-11 1.7350542715350947 1104 12-13 1.4879512170396296 1104 14-15 2.012564779883423 1104 16-17 0.6167039185817309 1104 18-19 -0.3102554237345707 1104 20-21 1.6910923772012723 1104 22-23 -0.004552875791812738 1104 24-25 0.5199850230602436 1104 26-27 0.334013336840151 1104 28-29 1.5955575915230895 1104 30-31 0.80188100005347 1104 32-33 -0.2695206545473141 1104 34-35 2.7745844520281615 1104 36-37 0.9767971707146543 1104 38-39 1.0888266942099776 1104 40-41 0.5220896137048925 1104 42-43 0.17431750314066008 1104 44-45 0.013300670570824735 1104 46-47 0.33392761532423165 1104 48-49 1.2714755118638585 1104 50-51 -0.5153714525253505 1104 52-53 1.7713134654109304 1104 54-55 0.9483770451773523 1104 56-57 -1.693922127902713 1104 58-59 -1.220648566979932 1104 60-61 1.4506275328587712 1104 62-63 -0.7522977084543712 1104 64-65 -0.7962563703122392 1104 66-67 -0.6554953473777267 1104 68-69 -2.8701352323133733 1104 70-71 0.09767248156126129 1104 72-73 -0.8211012559744404 1104 74-75 -1.651621426316808 1104 76 -1.1259002145699988 1105 1 -0.04328417187847222 1105 2 1.3025679409054227 1105 3 2.123257675489633 1105 4 2.7306952328736784 1105 5 0.38494405473216275 1105 6 1.2855431567909115 1105 7 1.1179700981415905 1105 8 0.6066791833728864 1105 9 -0.15896246729460373 1105 10-11 -1.794306193581182 1105 12-13 1.845800054248933 1105 14-15 2.2270996636043527 1105 16-17 0.5989713604421958 1105 18-19 0.9534073669631056 1105 20-21 1.1733598190617371 1105 22-23 0.9791680544407448 1105 24-25 0.1496361858509374 1105 26-27 -0.4968006166482226 1105 28-29 1.020848289197506 1105 30-31 0.7710670465651006 1105 32-33 1.7246653919643151 1105 34-35 0.35191003342351124 1105 36-37 0.13290182187744648 1105 38-39 0.8801057639774186 1105 40-41 1.4781942648676853 1105 42-43 2.3455384333732177 1105 44-45 0.6441146240591955 1105 46-47 1.1295671502079507 1105 48-49 2.5717662095382785 1105 50-51 1.8968378498002352 1105 52-53 2.173057651457441 1105 54-55 0.23907471959595483 1105 56-57 1.6781708953531016 1105 58-59 -0.051462520468305684 1105 60-61 1.344232184021564 1105 62-63 2.5168883380572566 1105 64-65 0.9473482808505516 1105 66-67 1.32851628052925 1105 68-69 0.1888763955936028 1105 70-71 0.19563759784032797 1105 72-73 0.19634060449067547 1105 74-75 1.5195995039157495 1105 76 1.7921230412439542 1106 1 -1.6149895982350593 1106 2 -0.45389200741498925 1106 3 0.0043946263940277674 1106 4 -2.2628448188059096 1106 5 -2.8159603380326956 1106 6 1.2687472911578368 1106 7 3.0275308216558017 1106 8 -2.8274293437588973 1106 9 -2.320461175304942 1106 10-11 0.8697764937573176 1106 12-13 0.7976734392618496 1106 14-15 0.1431203354389794 1106 16-17 -0.8319071925293784 1106 18-19 -0.6283109792901271 1106 20-21 0.6869257105346094 1106 22-23 1.262113790874853 1106 24-25 -0.05223719916197922 1106 26-27 -0.8937644409376304 1106 28-29 -1.2669424084769112 1106 30-31 0.3741032222756928 1106 32-33 0.8832571232304645 1106 34-35 1.0634733409170494 1106 36-37 -1.910702829285345 1106 38-39 -0.8611733057900253 1106 40-41 -0.009854830739548959 1106 42-43 -1.815960274637117 1106 44-45 0.652189559459714 1106 46-47 -1.4702390513424355 1106 48-49 0.7006421785305292 1106 50-51 1.2540729919190952 1106 52-53 -0.9286865345890689 1106 54-55 -0.9266229548226477 1106 56-57 -1.9300332390138237 1106 58-59 -0.3234263447577135 1106 60-61 -2.0049280226967845 1106 62-63 -0.5884088195654869 1106 64-65 0.8898547407988744 1106 66-67 0.49033798595560896 1106 68-69 0.28125365657551527 1106 70-71 -0.23427196288318441 1106 72-73 -2.7099901448633297 1106 74-75 -1.5724547596501388 1106 76 -0.10645577012555663 1107 1 -1.198322931568395 1107 2 0.06277465925167647 1107 3 0.7155057375051364 1107 4 -0.8517337076947982 1107 5 0.9007063286339729 1107 6 1.3020806244911718 1107 7 2.5164197105446924 1107 8 2.8392373229077705 1107 9 3.57953882469506 1107 10-11 3.6697764937573183 1107 12-13 1.5060067725951853 1107 14-15 2.665342557661198 1107 16-17 1.484759474137288 1107 18-19 0.09113346515432141 1107 20-21 1.992481266090163 1107 22-23 1.3065582353192973 1107 24-25 1.4227628008380222 1107 26-27 1.5367911146179267 1107 28-29 0.9913909248564217 1107 30-31 1.6602143333868042 1107 32-33 1.791590456563796 1107 34-35 0.9384733409170494 1107 36-37 2.7476305040479865 1107 38-39 0.463826694209974 1107 40-41 2.448478502593783 1107 42-43 1.1062619475851072 1107 44-45 3.443856226126382 1107 46-47 1.4714276153242274 1107 48-49 1.1978644007527528 1107 50-51 -0.9959270080809048 1107 52-53 1.2518690209664882 1107 54-55 1.4817103785106838 1107 56-57 0.7171889832083984 1107 58-59 2.5932403219089544 1107 60-61 2.8478497550809934 1107 62-63 -0.7384088195654854 1107 64-65 2.750965851909985 1107 66-67 0.7820046526222733 1107 68-69 0.4090314343532917 1107 70-71 0.2796169260057013 1107 72-73 1.8066765218033396 1107 74-75 2.3247674625720833 1107 76 -0.7453446590144424 1108 1 -0.08979579978544905 1108 2 0.4653586385798434 1108 3 1.5418623266524243 1108 4 0.009765000315539396 1108 5 2.4314556826391396 1108 6 -1.2725963780928105 1108 7 1.4435514934904283 1108 8 -1.532855700348044 1108 9 0.027084044333300028 1108 10-11 -0.22453875172071847 1108 12-13 2.322544240295443 1108 14-15 0.34337873337179303 1108 16-17 0.7268783371863812 1108 18-19 -0.3605461214089871 1108 20-21 1.0803365632477835 1108 22-23 0.2582378218826058 1108 24-25 1.940333860269547 1108 26-27 0.25901333684014816 1108 28-29 -0.9093842689420271 1108 30-31 0.061764720983703114 1108 32-33 0.2711770198712884 1108 34-35 -0.13646205959974722 1108 36-37 1.0166227521100062 1108 38-39 -1.3757081895109557 1108 40-41 -0.40552666536487436 1108 42-43 -0.8870197061616629 1108 44-45 -0.42565281780126796 1108 46-47 -0.8355491288618175 1108 48-49 -1.3352105346477678 1108 50-51 0.9084657567769767 1108 52-53 0.533522767736514 1108 54-55 0.48326076610758406 1108 56-57 0.306077872097287 1108 58-59 0.4717932934851845 1108 60-61 0.06516241657969957 1108 62-63 -2.9598558479892567 1108 64-65 -2.459628463335495 1108 66-67 -0.6133441845870315 1108 68-69 -0.12507709277848988 1108 70-71 -0.9671530998340927 1108 72-73 -1.0594733489976953 1108 74-75 0.6475064806599384 1108 76 -0.06834207503511536 1109 1 0.1933437350982743 1109 2 -0.042780896303877824 1109 3 -1.6761609291615294 1109 4 -1.2239559299170217 1109 5 0.4145952175228622 1109 6 0.03263618004672608 1109 7 -0.06691362278863977 1109 8 1.0892373229077705 1109 9 -2.4176833975271634 1109 10-11 -0.7149457284649046 1109 12-13 -0.9495487829603704 1109 14-15 -0.5999352201165777 1109 16-17 0.704203918581733 1109 18-19 1.2897445762654272 1109 20-21 -2.171407622798725 1109 22-23 -0.8670528757918099 1109 24-25 -2.205014976939758 1109 26-27 -1.5409866631598526 1109 28-29 -0.06694240847691191 1109 30-31 -0.82311899994653 1109 32-33 -0.7070206545473141 1109 34-35 0.2745844520281615 1109 36-37 0.5017971707146565 1109 38-39 -0.38617330579002385 1109 40-41 0.9595896137048925 1109 42-43 -0.9256824968593378 1109 44-45 0.8758006705708254 1109 46-47 -0.3035723846757712 1109 48-49 1.1589755118638614 1109 50-51 -0.20287145252535055 1109 52-53 2.0463134654109325 1109 54-55 1.6108770451773538 1109 56-57 0.5810778720972856 1109 58-59 0.27935143302006793 1109 60-61 1.1756275328587726 1109 62-63 1.7727022915456274 1109 64-65 -0.3712563703122349 1109 66-67 -0.6554953473777267 1109 68-69 -1.0951352323133747 1109 70-71 0.24767248156125987 1109 72-73 -0.07110125597444039 1109 74-75 -1.814121426316806 1109 76 0.6240997854300012 1110 1 -0.9566562649017278 1110 2 -0.09278089630387854 1110 3 -0.05116092916152937 1110 4 0.6510440700829783 1110 5 1.4395952175228608 1110 6 -2.342363819953274 1110 7 0.7830863772113581 1110 8 0.33923732290777053 1110 9 -0.017683397527164857 1110 10-11 -0.8399457284649046 1110 12-13 -1.5745487829603704 1110 14-15 -0.5749352201165792 1110 16-17 0.47920391858173517 1110 18-19 1.2772445762654314 1110 20-21 0.6660923772012737 1110 22-23 -1.4670528757918113 1110 24-25 1.219985023060243 1110 26-27 2.1590133368401467 1110 28-29 -0.6794424084769126 1110 30-31 1.9643810000534714 1110 32-33 -0.4695206545473134 1110 34-35 -1.9629155479718392 1110 36-37 0.8892971707146557 1110 38-39 -0.22367330579002598 1110 40-41 -0.10291038629510751 1110 42-43 1.6368175031406622 1110 44-45 -0.8991993294291767 1110 46-47 -1.603572384675772 1110 48-49 -2.06602448813614 1110 50-51 -0.515371452525347 1110 52-53 -1.8411865345890703 1110 54-55 -1.089122954822649 1110 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54-55 0.9483770451773523 2116 56-57 3.3775064435258564 2116 58-59 0.9329228615914928 2116 60-61 -0.9743724671412259 2116 62-63 0.05841657725991212 2116 64-65 1.2573150582591914 2116 66-67 0.7248617954794163 2116 68-69 1.3137933391151968 2116 70-71 0.09231533870411823 2116 72-73 -0.4568155416887265 2116 74-75 -0.2069785691739483 2116 76 0.12767121400142756 2117 1 -0.5941562649017271 2117 2 0.18846910369612146 2117 3 -0.08241092916152937 2117 4 -0.4677059299170203 2117 5 -1.1354047824771385 2117 6 -1.217363819953274 2117 7 0.8643363772113588 2117 8 1.6517373229077705 2117 9 -1.3864333975271634 2117 10-11 1.1006792715350961 2117 12-13 -1.7526737829603718 2117 14-15 3.0094397798834223 2117 16-17 2.5604539185817323 2117 18-19 -0.69775542373457 2117 20-21 0.18171737720127368 2117 22-23 -0.8889278757918113 2117 24-25 0.1543600230602422 2117 26-27 0.18088833684014816 2117 28-29 1.8893075915230888 2117 30-31 -0.8793689999465286 2117 32-33 -0.5820206545473141 2117 34-35 1.44020945202816 2117 36-37 0.573672170714655 2117 38-39 -0.09554830579002527 2117 40-41 1.262714613704894 2117 42-43 0.3524425031406615 2117 44-45 -1.3148243294291753 2117 46-47 1.3058026153242288 2117 48-49 2.0183505118638614 2117 50-51 1.0283785474746523 2117 52-53 -1.850561534589069 2117 54-55 -0.11412295482264767 2117 56-57 -0.11579712790271302 2117 58-59 2.173101433020065 2117 60-61 -0.15249746714122736 2117 62-63 1.6008272915456274 2117 64-65 0.5443686296877637 2117 66-67 0.7663796526222733 2117 68-69 -0.842010232313374 2117 70-71 0.14767248156125845 2117 72-73 -0.5086012559744404 2117 74-75 2.1515035736831933 2117 76 3.5428497854300005 2118 1 2.1448143233335664 2118 2 0.7895720448725925 2118 3 -0.5217491644556453 2118 4 1.6848675994947442 2118 5 3.1072422763463905 2118 6 0.4811655918114326 2118 7 2.0242628477995943 2118 8 -1.072527382974581 2118 9 0.869081308355188 2118 10-11 -0.29362219905313935 2118 12-13 -1.0789605476662558 2118 14-15 1.1463883092951868 2118 16-17 1.8674392126993808 2118 18-19 -0.5212848354992765 2118 20-21 -0.16111350515166833 2118 22-23 -0.3604352287329853 2118 24-25 0.6038085524720067 2118 26-27 1.3178368662519127 2118 28-29 -0.2890012320063242 2118 30-31 0.35041041181817434 2118 32-33 -0.8136383016061401 2118 34-35 2.520172687322276 2118 36-37 0.6701795236558326 2118 38-39 -0.33911448226061225 2118 40-41 0.2948837313519519 2118 42-43 -0.6999472027416935 2118 44-45 -1.3911110941350593 2118 46-47 0.5714276153242288 2118 48-49 1.1883872765697454 2118 50-51 -1.8962538054665252 2118 52-53 0.9169017007050471 2118 54-55 -1.4486817783520607 2118 56-57 0.188430813273758 2118 58-59 -1.1338838610975799 2118 60-61 1.2785687093293596 2118 62-63 -0.7420035908073146 2118 64-65 0.4110965708642347 2118 66-67 1.5025928879163892 2118 68-69 0.8482471206277999 2118 70-71 -1.9001216360857995 2118 72-73 2.0318399204961466 2118 74-75 0.5770550442714288 2118 76 -0.03619433221705748 2119 1 -1.8649895982350593 2119 2 -0.15944756297054496 2119 3 -1.7844942624948636 2119 4 1.6260440700829797 2119 5 -3.210404782477138 2119 6 -2.1423638199532746 2119 7 2.616419710544694 2119 8 2.6059039895744363 2119 9 -0.2760167308604977 2119 10-11 2.6142209382017647 2119 12-13 -0.48288211629370537 2119 14-15 -1.0457685534499106 2119 16-17 -1.2707960814182648 2119 18-19 1.30224457626543 2119 20-21 0.7702590438679415 2119 22-23 -0.12121954245847988 2119 24-25 2.4949850230602415 2119 26-27 -1.0076533298265176 2119 28-29 0.04139092485642237 2119 30-31 0.7935476667201371 2119 32-33 0.46381267878602017 2119 34-35 2.3662511186948265 2119 36-37 -0.4190361626186778 2119 38-39 1.4471600275433083 2119 40-41 -2.2570770529617725 2119 42-43 -0.004849163526007061 2119 44-45 2.3883006705708247 2119 46-47 0.688094281990896 2119 48-49 -0.37435782146947005 2119 50-51 -2.4403714525253477 2119 52-53 0.5963134654109297 2119 54-55 0.1817103785106866 2119 56-57 0.056077872097286985 2119 58-59 2.221018099686731 2119 60-61 1.658960866192107 2119 62-63 -2.9939643751210383 2119 64-65 0.5787436296877644 2119 66-67 0.3820046526222747 2119 68-69 0.32569810101995955 2119 70-71 -0.7148275184387387 2119 72-73 1.495565410692226 2119 74-75 -1.1141214263168067 2119 76 -0.7009002145699981 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 2.0 12 49.0 13 269.0 14 658.0 15 1140.0 16 1399.0 17 1664.0 18 1801.0 19 1721.0 20 1377.0 21 1116.0 22 787.0 23 536.0 24 372.0 25 255.0 26 207.0 27 134.0 28 90.0 29 67.0 30 44.0 31 40.0 32 35.0 33 21.0 34 17.0 35 14.0 36 18.0 37 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 6.199186991869919 28.15040650406504 29.202961672473865 36.44744483159118 2 17.81692174415247 23.765521224371934 32.442968524400804 25.97458850707479 3 5.046202714409471 26.81923187987294 42.289922032919435 25.844643372798153 4 3.746751371643084 26.08287611897199 23.1302339012417 47.04013860814323 5 2.7432861680623737 28.580710366734046 20.74790643950332 47.92809702570026 6 19.650591972278374 29.786312445856193 11.904418134565406 38.65867744730003 7 4.786312445856193 32.39965347964193 9.904706901530465 52.90932717297141 8 14.481663297718741 34.16835114062951 13.160554432572912 38.189431129078834 9 13.550389835402829 27.158533063817497 11.030898065261335 48.260179035518334 10-11 8.002454519203003 29.34594282414092 23.343199537972854 39.30840311868322 12-13 7.215564539416691 26.216430840311865 24.353883915680047 42.214120704591394 14-15 12.362835691596882 30.977476176725382 18.167051689286744 38.492636442390996 16-17 16.438059485994803 29.425353739532195 18.318654345942825 35.81793242853018 18-19 12.041582442968526 27.653046491481376 16.64380594859948 43.66156511695063 20-21 9.922754836846664 28.562662431417845 18.242853017614785 43.2717297141207 22-23 18.54966791799018 28.291943401674846 14.947300028876697 38.211088651458276 24-25 13.160554432572912 35.06352873231302 14.297574357493502 37.47834247762056 26-27 10.110453364135143 35.359514871498696 19.596448166329772 34.933583598036385 28-29 14.005197805371067 29.786312445856193 23.393733756858214 32.814755991914524 30-31 13.864423909904708 30.161709500433147 24.588507074790645 31.385359514871496 32-33 9.565405717585907 36.269130811435176 21.801905861969388 32.36355760900953 34-35 9.774761767253825 34.06728270285879 20.70459139474444 35.45336413514294 36-37 10.734911926075657 29.49393589373376 24.06872653768409 35.7024256425065 38-39 11.041726826451054 24.729280970257005 28.18365578977765 36.04533641351429 40-41 8.684666474155357 27.25238232746174 25.158821830782557 38.90412936760035 42-43 4.905428818943113 25.87712965636731 28.714265088073926 40.503176436615654 44-45 6.674126479930696 28.11146404851285 30.501010684377704 34.71339878717875 46-47 7.1289344498989315 31.94484550967369 25.693040716142075 35.2331793242853 48-49 7.29858504187121 33.55833092694196 19.993502743286168 39.149581287900666 50-51 7.3021946289344495 32.17585908172105 18.86370199249206 41.658244296852445 52-53 6.627201848108577 30.695928385792666 20.95726248917124 41.71960727692752 54-55 6.471989604389258 31.013572047357783 19.289633265954375 43.22480508229859 56-57 10.50750794109154 30.948599480219464 17.557031475599192 40.98686110308981 58-59 9.489604389257869 29.717730291654636 18.333092694195784 42.45957262489171 60-61 6.547790932717297 27.94542304360381 19.275194917701416 46.23159110597748 62-63 8.525844643372798 30.064250649725672 17.769997112330348 43.639907594571184 64-65 9.20805659832515 32.19751660410049 14.109875830205024 44.48455096736934 66-67 6.786023678891135 38.96549234767542 12.034363268842045 42.214120704591394 68-69 9.20083742419867 38.00173260179036 11.67701414958129 41.120415824429685 70-71 6.125469246318222 42.557031475599196 11.030898065261335 40.28660121282125 72-73 9.20805659832515 39.74155356627202 12.936760034652037 38.11362980075079 74-75 8.922899220329194 37.68408894022524 15.990470690153046 37.402541149292524 76 10.56165174704014 33.583598036384636 18.509962460294542 37.34478775628068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 1.0 12 1.0 13 2.5 14 4.5 15 4.5 16 5.0 17 6.0 18 3.5 19 5.0 20 13.0 21 17.0 22 15.0 23 18.5 24 28.0 25 32.0 26 42.5 27 51.5 28 58.5 29 67.0 30 67.5 31 88.0 32 120.5 33 133.0 34 163.0 35 208.0 36 258.5 37 294.0 38 325.5 39 390.0 40 480.0 41 569.0 42 601.0 43 649.0 44 750.0 45 823.0 46 843.0 47 890.5 48 919.5 49 882.0 50 863.0 51 830.0 52 787.5 53 731.5 54 685.0 55 636.0 56 565.0 57 491.0 58 439.0 59 413.5 60 348.5 61 281.0 62 253.0 63 212.5 64 159.0 65 132.0 66 105.5 67 93.0 68 77.0 69 56.5 70 47.0 71 42.0 72 31.5 73 15.5 74 8.5 75 7.0 76 9.5 77 9.0 78 7.5 79 9.0 80 7.5 81 5.0 82 4.5 83 5.0 84 4.5 85 4.5 86 5.5 87 6.0 88 3.0 89 1.0 90 3.0 91 3.5 92 3.0 93 1.5 94 1.5 95 3.0 96 3.0 97 2.5 98 3.5 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5486572336124748 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 13852.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.50187698527289 #Duplication Level Percentage of deduplicated Percentage of total 1 99.81861713705288 99.32139763211089 2 0.10157440325038089 0.20213687554143808 3 0.04353188710730611 0.12994513427663876 4 0.00725531451788435 0.028876696505919723 5 0.00725531451788435 0.036095870632399656 6 0.0 0.0 7 0.0 0.0 8 0.00725531451788435 0.057753393011839446 9 0.0 0.0 >10 0.0145106290357687 0.22379439792087782 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGGTC 16 0.11550678602367889 No Hit CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG 15 0.10828761189719896 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.007219174126479931 0.0 12 0.0 0.0 0.0 0.007219174126479931 0.0 13 0.0 0.0 0.0 0.007219174126479931 0.0 14 0.0 0.0 0.0 0.007219174126479931 0.0 15 0.0 0.0 0.0 0.007219174126479931 0.0 16 0.0 0.0 0.0 0.007219174126479931 0.0 17 0.0 0.0 0.0 0.007219174126479931 0.0 18 0.0 0.0 0.0 0.028876696505919723 0.0 19 0.0 0.0 0.0 0.04331504475887958 0.0 20 0.0 0.0 0.0 0.050534218885359514 0.0 21 0.0 0.0 0.0 0.050534218885359514 0.0 22 0.0 0.0 0.0 0.050534218885359514 0.0 23 0.0 0.0 0.0 0.050534218885359514 0.0 24 0.0 0.0 0.0 0.050534218885359514 0.0 25 0.0 0.0 0.0 0.050534218885359514 0.0 26 0.0 0.0 0.0 0.050534218885359514 0.0 27 0.0 0.0 0.0 0.06497256713831938 0.0 28 0.0 0.0 0.0 0.07219174126479931 0.0 29 0.0 0.0 0.0 0.07941091539127924 0.0 30 0.0 0.0 0.0 0.0938492636442391 0.0 31 0.0 0.0 0.0 0.10106843777071903 0.0 32 0.0 0.0 0.0 0.10828761189719896 0.0 33 0.0 0.0 0.0 0.10828761189719896 0.0 34 0.0 0.0 0.0 0.12994513427663876 0.0 35 0.0 0.0 0.0 0.14438348252959862 0.0 36 0.0 0.0 0.0 0.15160265665607855 0.0 37 0.0 0.0 0.0 0.17326017903551832 0.0 38 0.0 0.0 0.0 0.19491770141495812 0.0 39 0.0 0.0 0.0 0.21657522379439792 0.0 40 0.0 0.0 0.0 0.26710944267975745 0.0 41 0.0 0.0 0.0 0.2815477909327173 0.0 42 0.0 0.0 0.0 0.3032053133121571 0.0 43 0.0 0.0 0.0 0.31042448743863704 0.0 44 0.0 0.0 0.0 0.3393011839445567 0.0 45 0.0 0.0 0.0 0.34652035807103665 0.0 46 0.0 0.0 0.0 0.3970545769563962 0.0 47 0.0 0.0 0.0 0.4259312734623159 0.0 48 0.0 0.0 0.0 0.46202714409471557 0.0 49 0.0 0.0 0.0 0.48368466647415537 0.0 50 0.0 0.0 0.0 0.5053421888535952 0.0 51 0.0 0.0 0.0 0.5414380594859948 0.0 52 0.0 0.0 0.0 0.5630955818654346 0.0 53 0.0 0.0 0.0 0.6064106266243142 0.0 54 0.0 0.0 0.0 0.6425064972567138 0.0 55 0.0 0.0 0.0 0.6641640196361536 0.0 56 0.0 0.0 0.0 0.6786023678891134 0.0 57 0.0 0.0 0.0 0.729136586774473 0.0 58 0.0 0.0 0.0 0.7435749350274329 0.0 59 0.0 0.0 0.0 0.7507941091539128 0.0 60 0.0 0.0 0.0 0.7796708056598325 0.0 61 0.0 0.0 0.0 0.7941091539127924 0.0 62 0.0 0.0 0.0 0.8013283280392723 0.0 63 0.0 0.0 0.0 0.8157666762922322 0.0 64 0.0 0.0 0.0 0.8229858504187121 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATACC 20 0.0068086274 52.5 58 CCCATAC 45 0.0041645253 31.111109 57 ACTACTC 50 0.0069709225 27.999998 8 TTATACA 75 0.0017436239 23.333334 2 CTTATAC 95 2.594168E-4 22.105265 1 TATACAC 85 0.0036076817 20.588236 3 >>END_MODULE