FastQCFastQC Report
Wed 25 May 2016
SRR1780492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13852
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT358725.89517759168351No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT285720.625180479353162RNA PCR Primer, Index 15 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC9486.8437770719029745No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG4493.241409182789489No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC4343.1331215708922904No Hit
TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT2001.443834825295986No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT1661.1983829049956685No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC1100.7941091539127924No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT1040.7507941091539128No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT1020.7363557609009529No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG990.7146982385215132No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC980.7074790643950333No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT920.6641640196361537No Hit
CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC830.5991914524978342No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC810.5847531042448744No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT800.5775339301183945No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG750.5414380594859948No Hit
TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC710.512561362980075No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT560.40427375108287616No Hit
ACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCT490.35373953219751664RNA PCR Primer, Index 15 (95% over 23bp)
CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG430.310424487438637No Hit
CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC420.3032053133121571No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGT410.2959861391856772No Hit
CTTATACACATCTCCGAGCCCACGCGACCGCGGACAATCTCGTATGCCGT380.2743286168062374No Hit
CTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT370.26710944267975745No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGT330.23823274617383772No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGT320.23101357204735778No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG310.22379439792087782No Hit
ATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTGCTT270.19491770141495812RNA PCR Primer, Index 36 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGT260.1876985272884782No Hit
TCTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT260.1876985272884782No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGTCTT250.18047935316199826Illumina PCR Primer Index 4 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGTCTT250.18047935316199826TruSeq Adapter, Index 19 (95% over 21bp)
CTTATATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC240.17326017903551832No Hit
CTTATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGT240.17326017903551832No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCG230.16604100490903842No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCG230.16604100490903842No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC230.16604100490903842No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGTCTT220.15882183078255846Illumina PCR Primer Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGTCTT220.15882183078255846No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGT200.14438348252959862No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG190.1371643084031187No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATTTCGTATGCCGT190.1371643084031187No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTC190.1371643084031187No Hit
ATACACATCTCCGAGCCCACGAGAACGCGGACAATCTCGTATGCCGTCTT180.12994513427663876RNA PCR Primer, Index 36 (95% over 21bp)
ATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT170.12272596015015882RNA PCR Primer, Index 36 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGTC160.11550678602367889No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTC160.11550678602367889No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGACGT160.11550678602367889No Hit
ATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGTCTT160.11550678602367889RNA PCR Primer, Index 36 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAGCTCGTATGCCGT160.11550678602367889No Hit
CTTATACACATCTCCGAGCCCACGTGACCGCGGACAATCTCGTATGCCGT160.11550678602367889No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCG150.10828761189719896No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGT150.10828761189719896No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTATT150.10828761189719896No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGT150.10828761189719896No Hit
CTTCTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT150.10828761189719896No Hit
TCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT140.10106843777071904No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC140.10106843777071904No Hit
CTTATACACATCTCCGAGCCCAAGAGACCGCGGACAATCTCGTATGCCGT140.10106843777071904No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGT140.10106843777071904No Hit
CTTATACACATATCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT140.10106843777071904No Hit
ATACACCTCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT140.10106843777071904RNA PCR Primer, Index 15 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA1200.070.01
ACCACAA150.002178657670.070
TTATCAC150.002178657670.02
CCCACGC150.002178657670.019
AGTATGC150.002178657670.041
CTTATTA207.655769E-570.01
CTTATCA150.002178657670.01
TATCACA150.002178657670.03
AAGAACA207.655769E-570.070
ATCACAT150.002178657670.04
TCACATC150.002178657670.05
TCTTTAT207.655769E-570.01
TTTACAC1200.070.02
CTTATAC5950.061.1764681
TTATACA6150.058.048782
TTACACA1450.057.9310343
TTATTAC252.3092382E-455.9999962
ATTACAC252.3092382E-455.9999964
TATTACA252.3092382E-455.9999963
CAACCTC200.006808627452.532
Produced by FastQC (version 0.11.5)