##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780492_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13852 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62965636731158 34.0 34.0 34.0 31.0 34.0 2 33.23722206179613 34.0 34.0 34.0 32.0 34.0 3 33.442896332659544 34.0 34.0 34.0 34.0 34.0 4 33.50187698527289 34.0 34.0 34.0 34.0 34.0 5 33.49083164885937 34.0 34.0 34.0 34.0 34.0 6 37.3673115795553 38.0 37.0 38.0 37.0 38.0 7 36.91517470401386 38.0 37.0 38.0 35.0 38.0 8 36.92809702570026 38.0 37.0 38.0 35.0 38.0 9 36.58172105111175 38.0 37.0 38.0 34.0 38.0 10-11 36.3351140629512 38.0 37.0 38.0 34.0 38.0 12-13 35.96827172971412 38.0 36.5 38.0 32.5 38.0 14-15 35.90362402541149 38.0 36.0 38.0 31.0 38.0 16-17 35.84319953797285 38.0 36.0 38.0 31.0 38.0 18-19 36.00577533930118 38.0 36.5 38.0 32.0 38.0 20-21 35.859695350851865 38.0 36.0 38.0 31.0 38.0 22-23 35.82132544036962 38.0 36.0 38.0 32.5 38.0 24-25 34.74794253537395 37.0 34.5 38.0 28.0 38.0 26-27 34.94379872942535 37.0 34.0 38.0 31.0 38.0 28-29 34.7541149292521 37.5 35.0 38.0 28.0 38.0 30-31 33.92102223505631 37.0 34.0 38.0 23.5 38.0 32-33 34.611752815477914 37.0 34.0 38.0 25.0 38.0 34-35 33.45524112041582 37.0 32.5 38.0 23.0 38.0 36-37 33.28591539127923 37.0 34.0 38.0 22.0 38.0 38-39 31.02180190586197 35.5 27.0 37.0 11.0 38.0 40-41 33.02692751949177 36.5 32.5 37.5 23.0 38.0 42-43 34.737655212243716 37.0 34.5 38.0 28.5 38.0 44-45 35.09175570314756 37.0 35.0 38.0 29.0 38.0 46-47 35.06944845509673 37.0 35.0 38.0 28.5 38.0 48-49 34.579952353450764 37.0 35.0 38.0 27.5 38.0 50-51 34.4767181634421 37.0 34.0 38.0 26.0 38.0 52-53 34.112402541149294 37.0 34.0 38.0 24.0 38.0 54-55 34.55020935604967 37.0 34.5 38.0 25.5 38.0 56-57 34.35179035518337 37.0 34.0 38.0 24.5 38.0 58-59 34.08092694195784 37.0 34.0 38.0 24.0 38.0 60-61 34.15163875252671 37.0 34.0 38.0 24.5 38.0 62-63 34.945134276638754 37.0 35.0 38.0 28.0 38.0 64-65 34.78263066705169 37.0 35.0 38.0 26.5 38.0 66-67 32.49371931850996 36.5 31.0 38.0 21.5 38.0 68-69 27.814106266243144 32.5 20.5 37.0 11.0 38.0 70-71 23.436723938781405 24.5 11.0 33.5 10.0 37.0 72-73 19.505991914524976 19.0 11.0 25.0 10.0 33.5 74-75 18.92629223216864 19.0 11.0 25.0 10.0 33.0 76 19.50916835114063 19.0 11.0 25.0 10.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2183127055185352 1101 2 0.06000763614660798 1101 3 -0.5446590545332199 1101 4 -0.057142464119969816 1101 5 0.15822998986327974 1101 6 -1.7528052866056996 1101 7 0.29155676401512665 1101 8 0.17381915513830393 1101 9 0.27739829781943115 1101 10-11 -0.4718232002274476 1101 12-13 1.4319981537351296 1101 14-15 0.7414337586734803 1101 16-17 0.1321233646261888 1101 18-19 0.590106770024299 1101 20-21 0.17496014395678827 1101 22-23 0.2988444128915688 1101 24-25 -1.8922069397471688 1101 26-27 0.06468862756442917 1101 28-29 0.9221646660499232 1101 30-31 1.3547774798012497 1101 32-33 0.17864694608185516 1101 34-35 -1.8969012571782358 1101 36-37 0.7277200205265686 1101 38-39 0.22159056144971956 1101 40-41 1.1317974843330418 1101 42-43 1.308707732448262 1101 44-45 0.3480085504621755 1101 46-47 1.3100756059853609 1101 48-49 -3.1310239081174203 1101 50-51 -2.324574587507975 1101 52-53 -1.2417415742493034 1101 54-55 -1.0124082419733824 1101 56-57 -2.0063027030388554 1101 58-59 -2.948965207755027 1101 60-61 -2.7317351778375496 1101 62-63 -4.884531171107305 1101 64-65 -2.392108506519648 1101 66-67 -5.903922349595998 1101 68-69 -2.642924497946396 1101 70-71 -2.08503397524899 1101 72-73 -0.12061611229371749 1101 74-75 1.7892275712254815 1101 76 -1.5718862812148586 1102 1 -1.2551790649655388 1102 2 0.655399341215734 1102 3 0.5631750468492669 1102 4 0.45898656813809424 1102 5 0.5453267640568313 1102 6 0.6665495521039801 1102 7 0.6943217409736491 1102 8 0.5876440399309288 1102 9 1.210117191828651 1102 10-11 0.871494772122773 1102 12-13 0.6684036836890428 1102 14-15 1.3372862932356924 1102 16-17 0.6500957148565973 1102 18-19 0.6882634520519488 1102 20-21 1.4228864112378972 1102 22-23 1.22649418247682 1102 24-25 -5.527690810714915 1102 26-27 -1.2979841834954797 1102 28-29 1.0677867858655858 1102 30-31 3.454777479801251 1102 32-33 1.1122874990772544 1102 34-35 2.0372001253102425 1102 36-37 -3.1059205324688257 1102 38-39 0.514217289560321 1102 40-41 2.702765226268532 1102 42-43 1.235435843047341 1102 44-45 1.467824218664937 1102 46-47 1.721596343312548 1102 48-49 -4.9678902675644245 1102 50-51 -1.1854040805955322 1102 52-53 0.9928206377322581 1102 54-55 0.42307562899436135 1102 56-57 0.9678908453482435 1102 58-59 2.045044008834836 1102 60-61 -4.5243619059481475 1102 62-63 -1.470245456821587 1102 64-65 0.9853108483190596 1102 66-67 3.1403172817404084 1102 68-69 -0.6244913182228942 1102 70-71 -0.5417160028987595 1102 72-73 -1.4915838542292015 1102 74-75 -1.5158415531984843 1102 76 -0.34976646554665436 1103 1 1.3371554298079786 1103 2 0.7696850555014478 1103 3 0.13669421061233322 1103 4 0.4345963242356561 1103 5 0.1550828616178066 1103 6 0.15435443015275752 1103 7 0.7974576294753959 1103 8 0.5082015312201236 1103 9 0.6582008155220294 1103 10-11 -0.3671811860653733 1103 12-13 -1.2403071177046883 1103 14-15 -0.51741753951692 1103 16-17 -0.48196003427231204 1103 18-19 0.7325143231320865 1103 20-21 -0.405685017333532 1103 22-23 0.2846823357869326 1103 24-25 -4.974032274129549 1103 26-27 -0.5690643228683001 1103 28-29 -1.413397883123956 1103 30-31 1.842582357850027 1103 32-33 2.0736115408890967 1103 34-35 -0.5802214774772168 1103 36-37 -1.7891957937928673 1103 38-39 -0.2819499578264377 1103 40-41 1.453984738463653 1103 42-43 -0.03668959249272774 1103 44-45 0.3232249155290461 1103 46-47 1.0480771795494874 1103 48-49 -3.6121411386445637 1103 50-51 -1.0763448471460535 1103 52-53 0.764946073272327 1103 54-55 -0.3818024197861263 1103 56-57 0.6703298697384881 1103 58-59 -0.6995552943010495 1103 60-61 -5.276975146366269 1103 62-63 -1.9820921467170578 1103 64-65 -0.104933054119968 1103 66-67 -0.4680450875975737 1103 68-69 1.5427560685366863 1103 70-71 1.7147299901326 1103 72-73 -1.4671936103267633 1103 74-75 0.4308483422719007 1103 76 0.5728816180770409 1104 1 0.6341066493201737 1104 2 0.5196850555014478 1104 3 0.0988893325635587 1104 4 -0.21601343186190292 1104 5 -0.07967323594316866 1104 6 -0.058450447896021274 1104 7 0.5657503124022227 1104 8 0.04478689707378436 1104 9 1.0886886204000774 1104 10-11 -1.542790942162938 1104 12-13 0.26304654083190115 1104 14-15 0.9551434360928326 1104 16-17 0.5518814291423197 1104 18-19 1.186477737766232 1104 20-21 -1.5056850173335263 1104 22-23 -0.10922010323746889 1104 24-25 1.0098091892850931 1104 26-27 1.0913015307902398 1104 28-29 1.7338582144370136 1104 30-31 1.267277479801244 1104 32-33 0.6355017847915292 1104 34-35 0.16398583959595925 1104 36-37 2.3958651818168875 1104 38-39 3.369574432417462 1104 40-41 2.9277652262685265 1104 42-43 0.5550787001902009 1104 44-45 0.7713956472363677 1104 46-47 1.460882057598262 1104 48-49 -0.34110455327871136 1104 50-51 0.8056673479758913 1104 52-53 0.29817778058939837 1104 54-55 0.8730756289943642 1104 56-57 -0.14460915465176072 1104 58-59 -3.2763845625937336 1104 60-61 -1.3422190488052905 1104 62-63 0.46546882889269625 1104 64-65 0.8603108483190596 1104 66-67 -1.6043255754024486 1104 68-69 0.9683658246342475 1104 70-71 -0.2560017171844713 1104 72-73 -0.7040838542292001 1104 74-75 1.2198727325158032 1104 76 0.17166210588191788 1105 1 1.389339207459713 1105 2 0.7231734275944746 1105 3 0.4163311930286753 1105 4 -0.21543203651307152 1105 5 0.12672211289404345 1105 6 0.22468908698770207 1105 7 0.7169131030998983 1105 8 -0.9784689168797058 1105 9 0.9503165273768204 1105 10-11 0.7269764996975283 1105 12-13 -1.8046860173076382 1105 14-15 0.045259715162600855 1105 16-17 0.5597302663516146 1105 18-19 1.1088614586964667 1105 20-21 0.7222219594106534 1105 22-23 0.06403571071602698 1105 24-25 0.4862626776571801 1105 26-27 0.8418829261390712 1105 28-29 1.5335675167626022 1105 30-31 2.3082658518942694 1105 32-33 1.2259087615357203 1105 34-35 0.3375323512238637 1105 36-37 0.8781326236773523 1105 38-39 -0.12199533502439763 1105 40-41 0.3050908076638805 1105 42-43 1.292578700190198 1105 44-45 1.5126747170038115 1105 46-47 1.861463452947099 1105 48-49 -0.441976646301967 1105 50-51 1.5152603712317116 1105 52-53 0.8659103387289377 1105 54-55 1.0974942336455271 1105 56-57 0.9655652639528896 1105 58-59 0.1061735769411527 1105 60-61 1.9839437418923822 1105 62-63 -0.9542986129677686 1105 64-65 0.6574038715748713 1105 66-67 1.343639540876623 1105 68-69 0.14627280137843357 1105 70-71 2.427719213048082 1105 72-73 -0.5846071100431551 1105 74-75 -1.1417551744609398 1105 76 -0.30857045225761937 1106 1 -0.7075600173464913 1106 2 0.575240611057005 1106 3 0.48222266589689156 1106 4 0.1534310125825371 1106 5 -0.4268954581653901 1106 6 -0.25011711456268415 1106 7 -1.1286941320422201 1106 8 0.46145356374044866 1106 9 -0.7002002684888069 1106 10-11 -0.05112427549626375 1106 12-13 1.6533243186096769 1106 14-15 -1.0865232305738388 1106 16-17 0.5116036513645383 1106 18-19 0.17119995998845638 1106 20-21 0.8998705382220251 1106 22-23 1.1380021189847582 1106 24-25 0.26953141150731597 1106 26-27 0.6829681974569013 1106 28-29 0.6408026588814622 1106 30-31 1.271444146467914 1106 32-33 -0.04088710409735086 1106 34-35 0.17926361737373497 1106 36-37 0.988920737372446 1106 38-39 2.351518876861906 1106 40-41 2.669431892935201 1106 42-43 1.6814675890790838 1106 44-45 2.313062313903032 1106 46-47 1.341437613153822 1106 48-49 2.4213954467212915 1106 50-51 0.2542784590870113 1106 52-53 0.4356777805894012 1106 54-55 0.006408962327697054 1106 56-57 0.9901130675704692 1106 58-59 2.248615437406265 1106 60-61 1.943892062305821 1106 62-63 1.7821354955593591 1106 64-65 -0.46468915168094327 1106 66-67 0.26095220237532857 1106 68-69 0.9072547135231375 1106 70-71 -0.8171128282955884 1106 72-73 -0.5332505208958658 1106 74-75 -0.25512726748419823 1106 76 -0.7950045607847507 1107 1 1.0146622048757337 1107 2 -0.3858705000541107 1107 3 0.026667110341335842 1107 4 -0.11879120963968148 1107 5 0.07866009739016278 1107 6 0.4443273298817587 1107 7 0.6879725346244427 1107 8 0.2447868970737872 1107 9 -0.4946447129332512 1107 10-11 0.27943128005928486 1107 12-13 0.34776876305411974 1107 14-15 0.0356989916483883 1107 16-17 0.44215920692009547 1107 18-19 0.4184221822106835 1107 20-21 0.849870538222028 1107 22-23 0.1852243412069825 1107 24-25 -1.549913032937134 1107 26-27 -0.7948095803208801 1107 28-29 0.8296915477703593 1107 30-31 1.1103330353568097 1107 32-33 0.5424462292359777 1107 34-35 0.9403747284848478 1107 36-37 1.6972540707057746 1107 38-39 -0.6651477898047595 1107 40-41 -1.5194569959536928 1107 42-43 1.537023144634638 1107 44-45 0.06306231390303907 1107 46-47 1.9914376131538205 1107 48-49 1.8019510022768515 1107 50-51 1.77650068130923 1107 52-53 0.18012222503384123 1107 54-55 1.6786311845499213 1107 56-57 1.5345575120149064 1107 58-59 1.3069487707396021 1107 60-61 1.188336506750261 1107 62-63 1.293246606670472 1107 64-65 1.585310848319054 1107 66-67 -0.6612700198468957 1107 68-69 1.973921380189804 1107 70-71 1.9773316161488594 1107 72-73 -0.3249171875625372 1107 74-75 1.2809838436269132 1107 76 0.5105509947708065 1108 1 -0.16880032742400886 1108 2 0.653405985734004 1108 3 -0.16506415580853684 1108 4 0.24968424255669674 1108 5 0.5453267640568313 1108 6 -0.31019463394252966 1108 7 0.530866591471991 1108 8 -0.07149217269365948 1108 9 0.5317118762140325 1108 10-11 0.8897671973719525 1108 12-13 1.8348488664132887 1108 14-15 0.8708411105114422 1108 16-17 -0.1495720592297758 1108 18-19 0.5390940168360032 1108 20-21 1.315245215224607 1108 22-23 0.19194268746020526 1108 24-25 1.5909138404478824 1108 26-27 0.7139759493948858 1108 28-29 -0.3268975995164638 1108 30-31 0.8315216658477667 1108 32-33 0.16776922665199834 1108 34-35 0.5584625837820028 1108 36-37 1.8083651818168889 1108 38-39 2.1221907114872316 1108 40-41 0.8399745285941052 1108 42-43 -0.4050957184144508 1108 44-45 0.2219770425852019 1108 46-47 1.5591378715517479 1108 48-49 2.151046609511994 1108 50-51 2.305958045650314 1108 52-53 0.15660801314754025 1108 54-55 -0.6932034407730825 1108 56-57 0.38416991511568455 1108 58-59 1.0015224141504504 1108 60-61 2.2746414163109847 1108 62-63 1.8945385963345558 1108 64-65 1.389962011109759 1108 66-67 2.564569773434755 1108 68-69 2.890458847890063 1108 70-71 -0.3629784613705205 1108 72-73 1.0432998667010303 1108 74-75 0.5908029650739408 1108 76 -0.42484952202505966 1109 1 -2.7408933506798228 1109 2 -0.180314944498555 1109 3 0.5238893325635559 1109 4 -0.01601343186190718 1109 5 0.020326764056832758 1109 6 -1.0834504478960199 1109 7 -1.0342496875977787 1109 8 0.19478689707378294 1109 9 0.613688620400076 1109 10-11 -0.8802909421629366 1109 12-13 0.35054654083189973 1109 14-15 1.055143436092827 1109 16-17 0.9393814291423155 1109 18-19 1.248977737766232 1109 20-21 1.3068149826664666 1109 22-23 -0.34672010323746605 1109 24-25 -0.7401908107149069 1109 26-27 0.3038015307902384 1109 28-29 -2.3786417855629836 1109 30-31 0.8547774798012497 1109 32-33 0.5980017847915349 1109 34-35 0.8639858395959621 1109 36-37 0.4458651818168917 1109 38-39 2.432074432417462 1109 40-41 1.3027652262685265 1109 42-43 0.8425787001901952 1109 44-45 0.8463956472363705 1109 46-47 0.9983820575982634 1109 48-49 0.033895446721288636 1109 50-51 -0.15683265202410013 1109 52-53 -0.9643222194105974 1109 54-55 -0.9269243710056401 1109 56-57 0.6428908453482407 1109 58-59 -0.5263845625937336 1109 60-61 1.057780951194701 1109 62-63 0.7529688288926977 1109 64-65 0.7728108483190539 1109 66-67 1.5581744245975528 1109 68-69 -0.844134175365749 1109 70-71 -1.3810017171844713 1109 72-73 0.8959161457707978 1109 74-75 0.8323727325158004 1109 76 1.1966621058819165 1110 1 -1.315893350679822 1110 2 0.06968505550144499 1110 3 0.4988893325635573 1110 4 0.033986568138097084 1110 5 0.42032676405683134 1110 6 0.29154955210398015 1110 7 0.7157503124022213 1110 8 0.7947868970737844 1110 9 1.3386886204000774 1110 10-11 0.5447090578370677 1110 12-13 0.9130465408318997 1110 14-15 1.6051434360928312 1110 16-17 1.601881429142317 1110 18-19 0.5364777377662335 1110 20-21 1.1443149826664651 1110 22-23 -0.5717201032374604 1110 24-25 -0.6901908107149097 1110 26-27 1.453801530790237 1110 28-29 0.9963582144370164 1110 30-31 0.06727747980125542 1110 32-33 1.4480017847915292 1110 34-35 2.0389858395959592 1110 36-37 3.183365181816889 1110 38-39 2.8445744324174633 1110 40-41 0.07776522626853222 1110 42-43 1.0925787001901952 1110 44-45 2.133895647236365 1110 46-47 2.2483820575982634 1110 48-49 2.9838954467212915 1110 50-51 0.1306673479758942 1110 52-53 1.9231777805893984 1110 54-55 -0.7019243710056386 1110 56-57 2.2303908453482393 1110 58-59 -1.126384562593735 1110 60-61 0.3452809511947095 1110 62-63 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1.444040257464124 2116 64-65 -3.2432605802523717 2116 66-67 -1.3168255754024472 2116 68-69 -0.010205603937180285 2116 70-71 1.6439982828155273 2116 72-73 0.2941304314850832 2116 74-75 0.6270155896586616 2116 76 -0.9211950369752273 2117 1 0.7091066493201765 2117 2 0.6446850555014478 2117 3 0.33638933256355585 2117 4 0.30273656813809424 2117 5 -0.7359232359431687 2117 6 0.44779955210398015 2117 7 0.28450031240222273 2117 8 0.01353689707378436 2117 9 -2.061311379599921 2117 10-11 -0.7021659421629352 2117 12-13 -1.1244534591681017 2117 14-15 0.06451843609283259 2117 16-17 -2.585618570857683 2117 18-19 -2.691647262233765 2117 20-21 -0.811935017333532 2117 22-23 0.7282798967625368 2117 24-25 -3.5308158107149126 2117 26-27 -3.905573469209763 2117 28-29 -3.5848917855629807 2117 30-31 -2.7233475201987503 2117 32-33 -2.761373215208465 2117 34-35 1.1983608395959564 2117 36-37 -3.863509818183111 2117 38-39 -1.1866755675825367 2117 40-41 -1.4347347737314706 2117 42-43 0.40195370019019805 2117 44-45 -1.5098543527636323 2117 46-47 -2.932867942401735 2117 48-49 -2.1411045532787085 2117 50-51 -1.278707652024103 2117 52-53 1.0606777805894012 2117 54-55 -0.10817437100563865 2117 56-57 0.5803908453482407 2117 58-59 -1.282634562593735 2117 60-61 -1.8390940488052934 2117 62-63 0.41234382889269483 2117 64-65 0.33218584831905673 2117 66-67 -1.15745057540245 2117 68-69 -0.3410091753657518 2117 70-71 -2.487251717184474 2117 72-73 -0.9759588542292015 2117 74-75 -0.34887726748419823 2117 76 1.8404121058819172 2118 1 -0.2173639389151205 2118 2 0.38733211432497683 2118 3 -1.0275812556717412 2118 4 0.13545715637339129 2118 5 0.1335620581744763 2118 6 0.43125543445692216 2118 7 0.5657503124022227 2118 8 0.28008101472084235 2118 9 -1.7083702031293342 2118 10-11 -0.975143883339399 2118 12-13 -1.1832769885798697 2118 14-15 1.1683787302104776 2118 16-17 -4.556206806151799 2118 18-19 -0.32308108576317096 2118 20-21 -0.876273252627648 2118 22-23 0.8643093085272469 2118 24-25 1.3046621304615584 2118 26-27 -4.204286704503879 2118 28-29 -0.8183476679159227 2118 30-31 -0.6893401672575763 2118 32-33 0.7744723730268248 2118 34-35 1.5926623101841884 2118 36-37 -1.6916348181831111 2118 38-39 -1.0635138028766526 2118 40-41 0.23805934391558736 2118 42-43 1.321990464896082 2118 44-45 -2.5888984704106903 2118 46-47 0.5505879399512068 2118 48-49 0.5758072114271755 2118 50-51 -0.23918559320057398 2118 52-53 -2.44667516058707 2118 54-55 -1.6651596651232907 2118 56-57 0.9443614335835306 2118 58-59 -0.861678680240793 2118 60-61 -0.9025131664523514 2118 62-63 -0.4286488181661312 2118 64-65 0.8147226130249408 2118 66-67 -0.44329616363774704 2118 68-69 -3.746340057718694 2118 70-71 -0.33835465836094514 2118 72-73 -1.182760324817437 2118 74-75 -0.07130373807243373 2118 76 1.2393091647054462 2119 1 0.025773315986846512 2119 2 0.13635172216811497 2119 3 -0.7511106674364427 2119 4 -0.4743467651952358 2119 5 -0.3213399026098358 2119 6 -0.8334504478960199 2119 7 -1.0675830209311101 2119 8 -0.22187976959288136 2119 9 -0.8279780462665869 2119 10-11 -1.0761242754962694 2119 12-13 -0.9911201258347688 2119 14-15 -0.1531898972405017 2119 16-17 -0.652285237524346 2119 18-19 0.34064440443290067 2119 20-21 -2.1890183506668635 2119 22-23 -0.3592201032374618 2119 24-25 0.638975855951756 2119 26-27 -1.0670318025430916 2119 28-29 0.19635821443701929 2119 30-31 1.9714441464679169 2119 32-33 0.16466845145819775 2119 34-35 0.734819172929285 2119 36-37 0.0750318484835546 2119 38-39 -0.9762589009158695 2119 40-41 -1.9305681070648042 2119 42-43 -1.207421299809802 2119 44-45 0.5130623139030348 2119 46-47 -0.2974512757350638 2119 48-49 -0.38693788661204565 2119 50-51 1.4098340146425627 2119 52-53 -0.4809888860772702 2119 54-55 0.32307562899435993 2119 56-57 -2.698775821318428 2119 58-59 -0.6930512292603979 2119 60-61 0.7327809511947052 2119 62-63 -0.6011978377739737 2119 64-65 -0.864689151680949 2119 66-67 -0.48349224206911856 2119 68-69 1.1183658246342496 2119 70-71 -1.0393350505178098 2119 72-73 0.4417494791041321 2119 74-75 -0.49679393415086537 2119 76 -0.9783378941180843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 9.0 23 14.0 24 35.0 25 67.0 26 128.0 27 258.0 28 373.0 29 606.0 30 889.0 31 1210.0 32 1751.0 33 2357.0 34 3073.0 35 2511.0 36 526.0 37 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.8302050245451921 30.443257291365867 11.175281547790933 57.551256136298015 2 0.41149292520935604 2.9454230436038116 87.28703436326884 9.35604966791799 3 0.5703147559919145 35.05630955818654 61.76003465203581 2.613341033785735 4 0.2743286168062374 44.1741264799307 25.01443834825296 30.53710655501011 5 0.38261622870343637 52.93820386947733 42.975743574935024 3.7034363268842045 6 0.41149292520935604 46.02945423043604 8.367022812590239 45.19203003176437 7 0.8807392434305515 53.24862835691597 4.302627779382038 41.568004620271445 8 0.418712099335836 45.119838290499565 30.14727115218019 24.314178457984408 9 0.7724516315333526 24.314178457984408 3.1114640485128504 71.8019058619694 10-11 0.6858215420155934 25.664164019636154 42.896332659543745 30.753681778804502 12-13 15.218019058619694 3.349696794686688 33.13961882760612 48.2926653190875 14-15 24.03624025411493 16.45971700837424 6.555010106843777 52.94903263066705 16-17 32.51155067860237 27.718019058619692 0.6677736066993936 39.102656656078544 18-19 25.631677736066994 21.469823852151315 0.46924631822119545 52.4292520935605 20-21 17.405428818943115 10.33785734911926 0.5955818654345942 71.66113196650304 22-23 24.270863413225527 39.6765809991337 0.40427375108287616 35.6482818365579 24-25 32.735345076523245 44.888824718452206 0.8085475021657523 21.56728270285879 26-27 39.911204158244296 33.74602945423044 0.6966503032053133 25.646116084319953 28-29 26.205602079122148 8.006064106266244 0.9529309846953509 64.83540282991626 30-31 47.84507652324574 15.57897776494369 1.0576090095293098 35.51833670228126 32-33 52.01775916835114 24.238377129656367 1.1947733179324285 22.549090384060065 34-35 8.782125324862836 48.40817210511118 14.687409760323419 28.12229280970257 36-37 1.2236500144383482 52.38232746173838 23.595870632399656 22.798151891423622 38-39 15.192751949177014 11.059774761767253 31.06771585330638 42.67975743574935 40-41 9.976898642795264 16.041004909038406 41.015737799595726 32.966358648570605 42-43 37.60106843777072 9.839734334392146 45.1234478775628 7.435749350274329 44-45 11.843055154490326 23.462315911059775 33.7063239965348 30.9883049379151 46-47 39.29035518336702 2.707190297429974 21.368755414380594 36.63369910482241 48-49 29.114929252093564 1.1045336413514293 24.501876985272887 45.27866012128213 50-51 6.804071614207334 1.1081432284146695 48.375685821542014 43.71209933583598 52-53 14.878717874675138 1.3247184522090674 67.60756569448455 16.188997978631246 54-55 9.641207045913948 1.4113485417268266 49.62821253248628 39.31923187987294 56-57 37.62994513427664 1.8300606410626623 31.176003465203582 29.36399075945712 58-59 11.37741842333237 31.59832515160266 49.826739820964484 7.197516604100492 60-61 23.2204735778227 66.43444989893156 8.626913081143517 1.7181634421022236 62-63 2.295697372220618 95.26422177302916 0.7363557609009529 1.7037250938492638 64-65 0.3790066416401964 97.15925498123015 0.620848974877274 1.840889402252382 66-67 0.32847242275483685 94.53869477331793 0.9312734623159111 4.201559341611319 68-69 1.01429396477043 84.66647415535662 2.7793820386947736 11.53984984117817 70-71 2.3137453075368177 74.57406872653767 5.562373664452787 17.549812301472713 72-73 3.439936471267687 61.78891134854173 9.103378573491193 25.667773606699395 74-75 4.461449610164597 59.26941957840023 9.139474444123593 27.12965636731158 76 2.894888824718452 61.774473000288765 7.594571181056888 27.736066993935893 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 1.0 26 1.0 27 1.0 28 1.5 29 2.0 30 1.5 31 2.0 32 3.5 33 4.0 34 4.0 35 3.0 36 5.0 37 8.0 38 13.5 39 23.0 40 56.0 41 284.0 42 483.0 43 1306.5 44 2781.0 45 3327.5 46 3223.0 47 2670.0 48 1684.5 49 926.0 50 600.0 51 424.0 52 179.5 53 73.5 54 36.0 55 28.5 56 18.0 57 14.0 58 13.0 59 9.5 60 3.0 61 1.5 62 3.0 63 2.0 64 0.5 65 0.0 66 0.5 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 13852.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.254114929252093 #Duplication Level Percentage of deduplicated Percentage of total 1 77.99337434926645 11.897198960438926 2 9.46521533364884 2.887669650591972 3 3.1235210601041175 1.4293964770430263 4 1.419782300047326 0.8663008951775917 5 1.6564126833885469 1.263355472133988 6 0.7572172266919073 0.6930407161420733 7 0.37860861334595364 0.40427375108287616 8 0.473260766682442 0.5775339301183945 9 0.6152389966871746 0.8446433727981518 >10 3.218173213440606 9.168351140629511 >50 0.4259346900141978 5.306092982962749 >100 0.3312825366777094 11.298007507941092 >500 0.047326076668244205 6.8437770719029745 >1k 0.09465215333648841 46.52035807103667 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 3587 25.89517759168351 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT 2857 20.625180479353162 RNA PCR Primer, Index 15 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 948 6.8437770719029745 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 449 3.241409182789489 No Hit CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 434 3.1331215708922904 No Hit TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 200 1.443834825295986 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT 166 1.1983829049956685 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 110 0.7941091539127924 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT 104 0.7507941091539128 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT 102 0.7363557609009529 No Hit CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 99 0.7146982385215132 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 98 0.7074790643950333 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT 92 0.6641640196361537 No Hit CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 83 0.5991914524978342 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 81 0.5847531042448744 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT 80 0.5775339301183945 No Hit CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 75 0.5414380594859948 No Hit TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 71 0.512561362980075 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT 56 0.40427375108287616 No Hit ACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCT 49 0.35373953219751664 RNA PCR Primer, Index 15 (95% over 23bp) CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 43 0.310424487438637 No Hit CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 42 0.3032053133121571 No Hit CTTATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGT 41 0.2959861391856772 No Hit CTTATACACATCTCCGAGCCCACGCGACCGCGGACAATCTCGTATGCCGT 38 0.2743286168062374 No Hit CTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 37 0.26710944267975745 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGT 33 0.23823274617383772 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGT 32 0.23101357204735778 No Hit CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 31 0.22379439792087782 No Hit ATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTGCTT 27 0.19491770141495812 RNA PCR Primer, Index 36 (96% over 27bp) CTTATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGT 26 0.1876985272884782 No Hit TCTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 26 0.1876985272884782 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGTCTT 25 0.18047935316199826 Illumina PCR Primer Index 4 (95% over 22bp) ATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGTCTT 25 0.18047935316199826 TruSeq Adapter, Index 19 (95% over 21bp) CTTATATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 24 0.17326017903551832 No Hit CTTATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGT 24 0.17326017903551832 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCG 23 0.16604100490903842 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCG 23 0.16604100490903842 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC 23 0.16604100490903842 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGTCTT 22 0.15882183078255846 Illumina PCR Primer Index 7 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGTCTT 22 0.15882183078255846 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGT 20 0.14438348252959862 No Hit CCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 19 0.1371643084031187 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATTTCGTATGCCGT 19 0.1371643084031187 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTC 19 0.1371643084031187 No Hit ATACACATCTCCGAGCCCACGAGAACGCGGACAATCTCGTATGCCGTCTT 18 0.12994513427663876 RNA PCR Primer, Index 36 (95% over 21bp) ATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT 17 0.12272596015015882 RNA PCR Primer, Index 36 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGTC 16 0.11550678602367889 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTC 16 0.11550678602367889 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGACGT 16 0.11550678602367889 No Hit ATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGTCTT 16 0.11550678602367889 RNA PCR Primer, Index 36 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAGCTCGTATGCCGT 16 0.11550678602367889 No Hit CTTATACACATCTCCGAGCCCACGTGACCGCGGACAATCTCGTATGCCGT 16 0.11550678602367889 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCG 15 0.10828761189719896 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGT 15 0.10828761189719896 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTATT 15 0.10828761189719896 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGT 15 0.10828761189719896 No Hit CTTCTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 15 0.10828761189719896 No Hit TCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 14 0.10106843777071904 No Hit CTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC 14 0.10106843777071904 No Hit CTTATACACATCTCCGAGCCCAAGAGACCGCGGACAATCTCGTATGCCGT 14 0.10106843777071904 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGT 14 0.10106843777071904 No Hit CTTATACACATATCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 14 0.10106843777071904 No Hit ATACACCTCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT 14 0.10106843777071904 RNA PCR Primer, Index 15 (95% over 21bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.007219174126479931 0.0 12 0.0 0.0 0.0 0.007219174126479931 0.0 13 0.0 0.0 0.0 0.007219174126479931 0.0 14 0.0 0.0 0.0 0.007219174126479931 0.0 15 0.0 0.0 0.0 0.007219174126479931 0.0 16 0.0 0.0 0.0 0.007219174126479931 0.0 17 0.0 0.0 0.0 0.007219174126479931 0.0 18 0.0 0.0 0.0 0.028876696505919723 0.0 19 0.0 0.0 0.0 0.050534218885359514 0.0 20 0.0 0.0 0.0 0.06497256713831938 0.0 21 0.0 0.0 0.0 0.06497256713831938 0.0 22 0.0 0.0 0.0 0.06497256713831938 0.0 23 0.0 0.0 0.0 0.06497256713831938 0.0 24 0.0 0.0 0.0 0.06497256713831938 0.0 25 0.0 0.0 0.0 0.06497256713831938 0.0 26 0.0 0.0 0.0 0.06497256713831938 0.0 27 0.0 0.0 0.0 0.07219174126479931 0.0 28 0.0 0.0 0.0 0.07219174126479931 0.0 29 0.0 0.0 0.0 0.07941091539127924 0.0 30 0.0 0.0 0.0 0.08663008951775916 0.0 31 0.0 0.0 0.0 0.08663008951775916 0.0 32 0.0 0.0 0.0 0.08663008951775916 0.0 33 0.0 0.0 0.0 0.0938492636442391 0.0 34 0.0 0.0 0.0 0.0938492636442391 0.0 35 0.0 0.0 0.0 0.0938492636442391 0.0 36 0.0 0.0 0.0 0.0938492636442391 0.0 37 0.0 0.0 0.0 0.0938492636442391 0.0 38 0.0 0.0 0.0 0.10828761189719896 0.0 39 0.0 0.0 0.0 0.10828761189719896 0.0 40 0.0 0.0 0.0 0.12272596015015882 0.0 41 0.0 0.0 0.0 0.15160265665607855 0.0 42 0.0 0.0 0.0 0.1588218307825585 0.0 43 0.0 0.0 0.0 0.1660410049090384 0.0 44 0.0 0.0 0.0 0.1876985272884782 0.0 45 0.0 0.0 0.0 0.1876985272884782 0.0 46 0.0 0.0 0.0 0.209356049667918 0.0 47 0.0 0.0 0.0 0.23101357204735778 0.0 48 0.0 0.0 0.0 0.2743286168062374 0.0 49 0.0 0.0 0.0 0.2959861391856772 0.0 50 0.0 0.0 0.0 0.3320820098180768 0.0 51 0.0 0.0 0.0 0.3537395321975166 0.0 52 0.0 0.0 0.0 0.3609587063239965 0.0 53 0.0 0.0 0.0 0.38983540282991624 0.0 54 0.0 0.0 0.0 0.4259312734623159 0.0 55 0.0 0.0 0.0 0.43315044758879584 0.0 56 0.0 0.0 0.0 0.4475887958417557 0.0 57 0.0 0.0 0.0 0.47646549234767543 0.0 58 0.0 0.0 0.0 0.4909038406006353 0.0 59 0.0 0.0 0.0 0.5053421888535952 0.0 60 0.0 0.0 0.0 0.519780537106555 0.0 61 0.0 0.0 0.0 0.519780537106555 0.0 62 0.0 0.0 0.0 0.5269997112330349 0.0 63 0.0 0.0 0.0 0.5342188853595149 0.0 64 0.0 0.0 0.0 0.5414380594859948 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 120 0.0 70.0 1 ACCACAA 15 0.0021786576 70.0 70 TTATCAC 15 0.0021786576 70.0 2 CCCACGC 15 0.0021786576 70.0 19 AGTATGC 15 0.0021786576 70.0 41 CTTATTA 20 7.655769E-5 70.0 1 CTTATCA 15 0.0021786576 70.0 1 TATCACA 15 0.0021786576 70.0 3 AAGAACA 20 7.655769E-5 70.0 70 ATCACAT 15 0.0021786576 70.0 4 TCACATC 15 0.0021786576 70.0 5 TCTTTAT 20 7.655769E-5 70.0 1 TTTACAC 120 0.0 70.0 2 CTTATAC 595 0.0 61.176468 1 TTATACA 615 0.0 58.04878 2 TTACACA 145 0.0 57.931034 3 TTATTAC 25 2.3092382E-4 55.999996 2 ATTACAC 25 2.3092382E-4 55.999996 4 TATTACA 25 2.3092382E-4 55.999996 3 CAACCTC 20 0.0068086274 52.5 32 >>END_MODULE