FastQCFastQC Report
Wed 25 May 2016
SRR1780491_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780491_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91313
Sequences flagged as poor quality0
Sequence length50
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1300.14236746136913692No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC960.1051328945495165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGA250.002309357335.2990120
CTTCACA250.00234703135.1828961
TACACAG250.002359696435.144365
CTATACA702.7284841E-1134.554631
CGGTGTA451.0140557E-634.26194434
CTTTACA654.5838533E-1033.8297041
TTTACAC654.6020432E-1033.811172
TAAACAC401.821409E-532.9478383
CGGTGGT1350.032.55890343
ACGAGCA353.1935866E-431.43053224
TTACACA701.02591E-931.3788933
CTTATAA502.338853E-630.7850341
GAGTGTT300.005700482529.35126139
GGTGGTC1200.029.28696644
TCGGTGG1700.028.4567542
TCTTAAT554.9139144E-627.9863951
AGACATC407.0469844E-427.4565326
CTTATAC5750.025.6223241
TTATACA5900.024.9572262
CGACGCG450.001370157124.49972519
Produced by FastQC (version 0.11.5)