FastQCFastQC Report
Wed 25 May 2016
SRR1780491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91313
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT3478038.08877158783525No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT2338125.60533549439839RNA PCR Primer, Index 45 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC72837.9758632396263405No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG46195.058425415877258No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC38024.1637006778881425No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT18051.976717444394555No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC13161.4411967627829554No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC11071.212313690274112No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG8880.9724792745830275No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC7250.7939723807124944No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC6680.731549724573719No Hit
CTTATAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG6300.689934620481202No Hit
CTTAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC6140.672412471389616No Hit
ACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTTCT4090.44790993615366925RNA PCR Primer, Index 45 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC4000.4380537272896521No Hit
CTTATTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG3960.4336731900167556No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG3430.3756310711508766No Hit
CTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT3090.33839650433125623No Hit
CTTATCACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC3090.33839650433125623No Hit
ATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTTCTGCTT2730.29897166887518756RNA PCR Primer, Index 29 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTCTTC2520.27597384819248083RNA PCR Primer, Index 35 (95% over 22bp)
TCTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT2400.2628322363737912No Hit
TCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT2090.22888307250884318No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGT1940.21245605773548126No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTC1780.19493390864389518RNA PCR Primer, Index 12 (95% over 21bp)
CTTATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC1590.1741263565976367No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGTCTT1570.17193608796118845No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG1470.16098474477894711No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGC1310.14346259568736106No Hit
CTTACACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT1060.1160842377317578No Hit
TCTTAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT1050.11498910341353366No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTCTTC1020.11170370045886129TruSeq Adapter, Index 12 (95% over 24bp)
CTCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG930.10184749159484412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCTT302.4931032E-644.02301444
CTTTTAC207.8193744E-443.9989051
TATAACA850.043.9989053
TTATAAC850.043.9989052
TACACAC207.8193744E-443.9989053
TTTTACA207.8193744E-443.9989052
ATAACAC800.043.9989054
CTTACAC408.1709E-943.9989051
TCTTTAC254.4112676E-543.9989051
TTATCAC302.5025347E-643.99892
CTTATCA302.5025347E-643.99891
TCTTCTG302.5025347E-643.998941
TCTTTAT1200.043.99891
CTTTACA7900.042.328061
CCGTCTT24450.041.86237744
TCTTAAT950.041.683171
CTTAACA850.041.4107321
TTAACAC850.041.4107322
TTTACAC8100.041.2829252
CTATACA4100.040.24291
Produced by FastQC (version 0.11.5)