FastQCFastQC Report
Wed 25 May 2016
SRR1780490_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780490_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12210
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGGTC260.21294021294021295No Hit
CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG180.14742014742014742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTG150.002173380269.99180611
TAACGCT150.002173380269.99180610
CTAACGC150.002173380269.9918069
CCGAAAA200.006792275752.49385527
GAAAAAC400.00232847234.99590329
CATCTGA802.217388E-630.6214149
ACATCTG802.217388E-630.6214148
ATCTGAC905.551512E-627.21903410
TCTGACG958.4515395E-625.78645511
TGACGCT851.2093336E-424.7029913
CACATCT1051.8365987E-523.33067
CTGACGC1051.8365987E-523.330612
GACGCTG800.00252204421.87243814
CGCTGCC900.00499261419.44216713
Produced by FastQC (version 0.11.5)