##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780490_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12210 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.324897624897623 21.0 12.0 23.0 12.0 32.0 2 18.16764946764947 12.0 11.0 23.0 11.0 32.0 3 17.98927108927109 11.0 11.0 23.0 11.0 32.0 4 18.126945126945127 11.0 11.0 23.0 11.0 32.0 5 18.756511056511055 11.0 11.0 23.0 11.0 33.0 6 20.943325143325144 21.0 11.0 31.0 11.0 35.0 7 21.644226044226045 21.0 11.0 31.0 11.0 36.0 8 22.03923013923014 22.0 11.0 32.0 11.0 36.0 9 22.226208026208027 21.0 12.0 33.0 12.0 36.0 10-11 20.574856674856676 21.0 11.0 29.5 11.0 35.0 12-13 20.417076167076168 21.0 11.0 28.0 11.0 34.0 14-15 20.294062244062246 21.0 11.0 29.5 11.0 34.0 16-17 19.819410319410316 16.5 11.0 27.5 11.0 34.0 18-19 20.074897624897623 21.0 11.0 28.0 11.0 34.0 20-21 20.20986895986896 16.0 11.0 29.0 11.0 34.0 22-23 19.796273546273547 16.0 11.0 29.0 10.5 34.0 24-25 19.806265356265357 16.0 11.0 27.5 10.5 34.0 26-27 19.409213759213756 11.5 11.0 26.5 10.5 34.0 28-29 19.025470925470927 11.0 11.0 26.0 11.0 34.0 30-31 18.9009009009009 11.0 11.0 26.0 11.0 34.0 32-33 19.274447174447175 11.0 11.0 26.0 11.0 34.0 34-35 19.417895167895168 16.0 11.0 26.5 11.0 34.0 36-37 19.30806715806716 11.0 11.0 26.0 11.0 34.0 38-39 18.72088452088452 11.0 11.0 26.0 11.0 34.0 40-41 19.039926289926292 11.0 11.0 26.0 11.0 34.0 42-43 19.203808353808355 11.0 11.0 26.0 11.0 34.0 44-45 18.935094185094187 11.0 11.0 26.0 11.0 34.0 46-47 19.04807534807535 11.0 11.0 26.0 11.0 34.0 48-49 19.258681408681408 15.0 11.0 26.0 11.0 34.0 50-51 19.353194103194102 15.0 11.0 26.0 11.0 34.0 52-53 19.45864045864046 16.0 11.0 26.0 11.0 34.0 54-55 19.468796068796067 20.0 11.0 26.0 11.0 34.0 56-57 18.932882882882883 15.0 11.0 26.0 11.0 33.5 58-59 18.928583128583128 16.0 11.0 26.0 11.0 33.5 60-61 19.096273546273547 19.0 11.0 26.0 11.0 33.0 62-63 18.85802620802621 15.0 11.0 26.0 11.0 33.0 64-65 18.93820638820639 15.0 11.0 26.0 11.0 33.0 66-67 19.48042588042588 19.0 11.0 26.0 11.0 33.0 68-69 19.106879606879605 15.0 11.0 26.0 11.0 33.0 70-71 19.519901719901718 19.0 11.0 26.0 11.0 33.5 72-73 18.86879606879607 19.0 11.0 25.0 11.0 33.0 74-75 18.550573300573298 15.0 11.0 25.0 11.0 32.5 76 17.476330876330877 11.0 11.0 24.0 11.0 31.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.834542467256469 1101 2 1.0221854959439618 1101 3 -0.3528602829983214 1101 4 -0.03382042851145428 1101 5 1.5735130258299677 1101 6 1.7279726304892762 1101 7 -2.5817314214311367 1101 8 0.10119888211259465 1101 9 0.6838025200377267 1101 10-11 -0.014732633330094558 1101 12-13 -0.3046308830491036 1101 14-15 -0.17032813465104724 1101 16-17 -0.36079721615477567 1101 18-19 -0.6426311238280711 1101 20-21 0.4936418991131042 1101 22-23 -0.422459667920112 1101 24-25 2.872418323139833 1101 26-27 2.193544006595342 1101 28-29 0.7344687441943307 1101 30-31 -1.9511962418495656 1101 32-33 -0.1417447018782667 1101 34-35 -1.2116668001293185 1101 36-37 -0.2986440730384352 1101 38-39 0.9929905058909085 1101 40-41 2.3351331008002596 1101 42-43 -0.25692109436713295 1101 44-45 -0.44139935120067264 1101 46-47 -0.8252224702292068 1101 48-49 -0.914108467391042 1101 50-51 0.7609695790279645 1101 52-53 -0.09348485004482399 1101 54-55 1.4425365300534558 1101 56-57 0.9090500772378078 1101 58-59 -1.3786086126888968 1101 60-61 -1.8040842897788174 1101 62-63 2.070174912059361 1101 64-65 -0.2732933723667017 1101 66-67 -1.5691443336228197 1101 68-69 -1.77780588060115 1101 70-71 -1.1696966795271848 1101 72-73 -0.699913068754455 1101 74-75 -1.3892055105416503 1101 76 1.5582795408116574 1102 1 -0.06787580058980325 1102 2 3.076352162610629 1102 3 2.4929730503350136 1102 4 1.1224295714885457 1102 5 0.2818463591632998 1102 6 1.2508892971559433 1102 7 -2.494231421431138 1102 8 1.074115548779261 1102 9 -0.7682808132956076 1102 10-11 0.21026736666990686 1102 12-13 0.4714107836175643 1102 14-15 -0.4953281346510465 1102 16-17 0.6902444505118908 1102 18-19 1.719868876171926 1102 20-21 0.568641899113107 1102 22-23 2.194206998746555 1102 24-25 1.4745016564731657 1102 26-27 0.6466690065953422 1102 28-29 1.3375937441943293 1102 30-31 1.745678758150433 1102 32-33 -0.19278636854493314 1102 34-35 1.6487498665373472 1102 36-37 0.9294809269615634 1102 38-39 1.5679905058909078 1102 40-41 0.06846643413359388 1102 42-43 1.0805789056328656 1102 44-45 0.9867256487993288 1102 46-47 -0.4866808035625425 1102 48-49 1.7004748659422937 1102 50-51 2.0422195790279645 1102 52-53 0.8669318166218432 1102 54-55 -0.8407968032798756 1102 56-57 0.3559250772378064 1102 58-59 0.8536830539777718 1102 60-61 1.619874043554514 1102 62-63 -1.2485750879406403 1102 64-65 1.542331627633299 1102 66-67 0.5871056663771803 1102 68-69 -1.377805880601148 1102 70-71 -0.833238346193852 1102 72-73 -0.028038068754455026 1102 74-75 0.953502822791684 1102 76 0.8541128741449917 1103 1 -1.117875800589804 1103 2 0.8638521626106304 1103 3 0.15547305033501502 1103 4 -0.6088204285114536 1103 5 -1.8431536408367002 1103 6 0.5446392971559426 1103 7 -1.2317314214311388 1103 8 1.2178655487792618 1103 9 0.4254691867043938 1103 10-11 -0.5897326333300938 1103 12-13 0.6370357836175629 1103 14-15 0.8546718653489549 1103 16-17 0.6058694505118893 1103 18-19 0.8698688761719247 1103 20-21 0.03114189911310561 1103 22-23 0.2817069987465537 1103 24-25 0.4307516564731664 1103 26-27 -0.5439559934046585 1103 28-29 0.10946874419433072 1103 30-31 -0.6011962418495678 1103 32-33 -0.8334113685449331 1103 34-35 1.1424998665373458 1103 36-37 -3.0111440730384373 1103 38-39 0.8804905058909078 1103 40-41 0.36846643413359104 1103 42-43 0.6055789056328642 1103 44-45 -1.2163993512006712 1103 46-47 -0.14605580356253967 1103 48-49 -0.3307751340577063 1103 50-51 -0.46403042097203695 1103 52-53 -0.464318183378154 1103 54-55 -1.9657968032798756 1103 56-57 -2.228449922762195 1103 58-59 -1.2494419460222268 1103 60-61 1.7792490435545147 1103 62-63 -0.7798250879406403 1103 64-65 -2.4982933723666996 1103 66-67 -1.1191443336228168 1103 68-69 -1.5403058806011494 1103 70-71 -0.048863346193854795 1103 72-73 -1.6874130687544557 1103 74-75 -2.2058721772083167 1103 76 -0.5333871258550076 1104 1 2.321013088299086 1104 2 -1.027814504056039 1104 3 1.5693619392239029 1104 4 -0.7282648729558971 1104 5 -1.1209314186144788 1104 6 0.5390837416003862 1104 7 -2.5706203103200274 1104 8 -0.9432455623318496 1104 9 0.39491363114883526 1104 10-11 0.418600700003239 1104 12-13 -2.1379642163824357 1104 14-15 -1.8564392457621572 1104 16-17 -0.6969083272658878 1104 18-19 -0.27318667938363106 1104 20-21 0.6519752324464392 1104 22-23 0.4025403320798908 1104 24-25 -0.34147056574905577 1104 26-27 -0.7231226600713256 1104 28-29 -0.5183090335834493 1104 30-31 0.5738037581504329 1104 32-33 -1.1806335907671546 1104 34-35 1.8466665332040115 1104 36-37 0.3374670380726741 1104 38-39 0.90132383922424 1104 40-41 -1.2787557880886276 1104 42-43 0.004190016743976344 1104 44-45 1.7419339821326645 1104 46-47 1.1608886408819004 1104 48-49 -0.23355291183548488 1104 50-51 1.3859695790279645 1104 52-53 0.21762626106628602 1104 54-55 -2.1602412477243185 1104 56-57 -1.5798388116510829 1104 58-59 -0.38416416824444966 1104 60-61 -2.0485287342232645 1104 62-63 -0.3076028657184189 1104 64-65 -0.37607115014447956 1104 66-67 -2.0135887780672626 1104 68-69 -1.6000281028233694 1104 70-71 0.22752554269503733 1104 72-73 -0.10269084653223359 1104 74-75 -1.1280943994305375 1104 76 1.180501763033881 1105 1 -0.21493462411921627 1105 2 -1.111147837389371 1105 3 -0.9371740084885154 1105 4 0.8779442773708972 1105 5 0.9362581238691838 1105 6 2.153462826567708 1105 7 -1.917025539078196 1105 8 0.8149243723086741 1105 9 -0.5201190485897236 1105 10-11 0.5264438372581424 1105 12-13 2.2590946071469773 1105 14-15 1.95320127711366 1105 16-17 0.04410474462953573 1105 18-19 -2.0338075944163094 1105 20-21 0.32599484028957804 1105 22-23 -0.6293224130181478 1105 24-25 -1.9207189317621314 1105 26-27 -0.6741030522281868 1105 28-29 1.2962334500766843 1105 30-31 1.595862581679846 1105 32-33 -0.9142937214861107 1105 34-35 -1.0332354275802977 1105 36-37 -1.0309970142149076 1105 38-39 -0.4981859646973277 1105 40-41 -1.3727100364546416 1105 42-43 -0.7098622708377214 1105 44-45 -0.4590464100242002 1105 46-47 -2.070320509444894 1105 48-49 -0.1837163105282933 1105 50-51 1.8933225202044355 1105 52-53 0.7878876989747852 1105 54-55 -0.6202085679857596 1105 56-57 -1.2056558051151356 1105 58-59 0.4998227598601268 1105 60-61 -0.903103897621957 1105 62-63 0.44076314735347566 1105 64-65 -0.7968227841314075 1105 66-67 -0.3926737453875262 1105 68-69 -1.274864704130561 1105 70-71 -2.654010405017381 1105 72-73 -1.096971892283868 1105 74-75 0.07942194043874196 1105 76 -1.4436812435020663 1106 1 -1.1303758005898032 1106 2 -2.517397837389371 1106 3 -1.7570269496649864 1106 4 -4.533820428511454 1106 5 -2.2494036408367 1106 6 -2.5303607028440567 1106 7 -2.744231421431138 1106 8 -1.550884451220739 1106 9 -3.6432808132956076 1106 10-11 -2.883482633330093 1106 12-13 -2.2160892163824357 1106 14-15 -1.7453281346510465 1106 16-17 -0.8722555494881092 1106 18-19 -0.9363811238280739 1106 20-21 0.13114189911310703 1106 22-23 -1.6807930012534449 1106 24-25 -2.2442483435268343 1106 26-27 -2.103330993404658 1106 28-29 -0.9905312558056707 1106 30-31 -1.519946241849567 1106 32-33 -0.05216136854493314 1106 34-35 -1.5387501334626528 1106 36-37 -2.3673940730384366 1106 38-39 -1.3538844941090922 1106 40-41 -0.4784085658664061 1106 42-43 -2.3412960943671344 1106 44-45 -0.04452435120067122 1106 46-47 -0.5179308035625425 1106 48-49 -3.2995251340577063 1106 50-51 1.2297195790279645 1106 52-53 0.9606818166218432 1106 54-55 -1.5595468032798756 1106 56-57 -2.4409499227621936 1106 58-59 -3.4588169460222282 1106 60-61 -0.20825095644548597 1106 62-63 -0.9985750879406403 1106 64-65 -1.895168372366701 1106 66-67 -2.3347693336228197 1106 68-69 -0.690305880601148 1106 70-71 0.744886653806148 1106 72-73 -1.621788068754455 1106 74-75 0.07850282279168397 1106 76 -0.9583871258550083 1107 1 1.4847557783575667 1107 2 -0.4006215215998985 1107 3 0.6804730503350136 1107 4 1.045126939909597 1107 5 3.038425306531721 1107 6 1.2788498234717345 1107 7 -0.36594194774692923 1107 8 -0.0952923459575814 1107 9 0.7925744498622862 1107 10-11 -0.046311580698514376 1107 12-13 0.6909831520386192 1107 14-15 2.090198181138426 1107 16-17 0.45586945051189076 1107 18-19 0.1442109814350836 1107 20-21 0.4567997938499495 1107 22-23 1.4310491040097126 1107 24-25 3.242593761736323 1107 26-27 2.0488071644900785 1107 28-29 1.3844687441943293 1107 30-31 1.8172248107820117 1107 32-33 0.7521149472445394 1107 34-35 0.7655261823268198 1107 36-37 1.4171454006457758 1107 38-39 0.5350957690488016 1107 40-41 0.7526769604493815 1107 42-43 3.2878157477381293 1107 44-45 1.6191269645888013 1107 46-47 3.170391564858509 1107 48-49 2.550803813310715 1107 50-51 1.9846537895542795 1107 52-53 0.5626555008323706 1107 54-55 2.093413723035912 1107 56-57 1.8879974456588613 1107 58-59 0.8775317381882992 1107 60-61 1.2687227277650415 1107 62-63 2.509648596269887 1107 64-65 2.500390838159614 1107 66-67 0.022960929535074115 1107 68-69 -1.0620164069169356 1107 70-71 0.9504787590693056 1107 72-73 -0.0025446477018249425 1107 74-75 0.9493909806864238 1107 76 0.923191821513413 1108 1 0.7564485237345195 1108 2 -3.6787154049569395 1108 3 -0.30601343615147414 1108 4 -0.38517177986280515 1108 5 -1.1877482354312932 1108 6 -3.9594147568981093 1108 7 -1.0871368268365451 1108 8 -0.6969993160856021 1108 9 2.058577294812501 1108 10-11 -0.1782461468436054 1108 12-13 -1.4622885407067585 1108 14-15 -2.1068146211375307 1108 16-17 -0.5441305494881092 1108 18-19 -0.23452301571996514 1108 20-21 -0.7928445873733772 1108 22-23 -0.6436308390912835 1108 24-25 0.08683273755424636 1108 26-27 1.4300304930818264 1108 28-29 -1.5817474720218883 1108 30-31 -1.7876827283360512 1108 32-33 -1.5462492063827717 1108 34-35 1.4536485151859964 1108 36-37 1.317572143177781 1108 38-39 -1.6786311157307132 1108 40-41 -1.282884917217757 1108 42-43 -0.9717859592319975 1108 44-45 -0.34815610795742913 1108 46-47 -1.726123371130111 1108 48-49 0.6489545956720235 1108 50-51 0.5109695790279645 1108 52-53 1.0772358706758993 1108 54-55 -1.2732292357123107 1108 56-57 -0.015274247086516368 1108 58-59 -2.5741041081843896 1108 60-61 -1.6153455510400825 1108 62-63 0.08503977692422282 1108 64-65 -0.04761769669102378 1108 66-67 -0.08941460389308986 1108 68-69 -0.12104912384439004 1108 70-71 -2.8539309137614204 1108 72-73 -1.7607238795652655 1108 74-75 0.3711548498187085 1108 76 -0.07325199071987143 1109 1 0.6821241994101968 1109 2 2.055518829277297 1109 3 1.3749174947794565 1109 4 2.771735127044103 1109 5 0.6568463591632998 1109 6 1.4835281860448326 1109 7 1.2071574674577512 1109 8 -2.082134451220739 1109 9 -2.4106419244067183 1109 10-11 0.21026736666990686 1109 12-13 0.62592467250645 1109 14-15 -1.5786614679843822 1109 16-17 0.8030916727341122 1109 18-19 0.9212577650608154 1109 20-21 0.19364189911310703 1109 22-23 -0.6807930012534449 1109 24-25 -1.4248038990823915 1109 26-27 1.1935440065953422 1109 28-29 0.5928020775276615 1109 30-31 1.6154704248171008 1109 32-33 -0.625078035211601 1109 34-35 1.12444431098179 1109 36-37 -1.6347551841495473 1109 38-39 0.49854606144646496 1109 40-41 1.0545775452447046 1109 42-43 1.1430789056328656 1109 44-45 -0.20251046231178194 1109 46-47 0.11922197421523606 1109 48-49 0.1553359770534044 1109 50-51 -0.836252643194257 1109 52-53 -0.03237373893371398 1109 54-55 -0.5352412477243185 1109 56-57 0.6562722994600279 1109 58-59 -0.9952752793555604 1109 60-61 0.10424904355451403 1109 62-63 -0.015936199051751032 1109 64-65 0.5822621831888561 1109 66-67 -0.8191443336228197 1109 68-69 -1.58613921393448 1109 70-71 1.3108588760283695 1109 72-73 0.03619804235665569 1109 74-75 1.1357944894583483 1109 76 -1.319498236966119 1110 1 -0.45676468947869253 1110 2 -3.194481170722705 1110 3 0.20825082811279216 1110 4 -2.6727093174003436 1110 5 -0.5653758630589216 1110 6 1.2890837416003862 1110 7 0.6516019119021941 1110 8 1.2789766598903718 1110 9 -0.16064192440671832 1110 10-11 3.4463784777810176 1110 12-13 1.1398135613953428 1110 14-15 0.5185607542378392 1110 16-17 0.9142027838452265 1110 18-19 0.39347998728304034 1110 20-21 1.51308634355755 1110 22-23 -0.611348556809002 1110 24-25 -0.6748038990823915 1110 26-27 1.4713217843731208 1110 28-29 0.6344687441943293 1110 30-31 0.7960259803726544 1110 32-33 -1.3473002574338224 1110 34-35 -1.458889022351542 1110 36-37 0.21246703807267409 1110 38-39 -1.2653428274424243 1110 40-41 -2.667644676977517 1110 42-43 0.5736344611884228 1110 44-45 0.29748953768821806 1110 46-47 0.3831108631041218 1110 48-49 -0.9696640229465956 1110 50-51 0.6915251345835216 1110 52-53 -2.476818183378157 1110 54-55 -0.3824634699465399 1110 56-57 -1.3576165894288614 1110 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2116 62-63 -0.07292853621650508 2116 64-65 1.6387755931505374 2116 66-67 -0.23293743707109726 2116 68-69 -0.9122886392218383 2116 70-71 0.8170849296682192 2116 72-73 0.8776731381420966 2116 74-75 0.48205885727444553 2116 76 2.96402666724844 2117 1 0.8804000614791612 2117 2 -2.2145961132514405 2117 3 -0.9574579841477444 2117 4 0.6213519852816489 2117 5 -0.2828088132504938 2117 6 3.922225504052495 2117 7 2.7665444406378263 2117 8 -0.17696203742763572 2117 9 1.7435726349802536 2117 10-11 -0.7681809091921643 2117 12-13 -0.9310676646583005 2117 14-15 0.8107063481075727 2117 16-17 -1.251027101212248 2117 18-19 -1.946079399690145 2117 20-21 -0.34084085950758336 2117 22-23 0.9700690677120711 2117 24-25 2.7298895875076497 2117 26-27 -3.0219732347839674 2117 28-29 -0.4172553937367063 2117 30-31 -3.577058310815083 2117 32-33 0.13641621766196366 2117 34-35 -1.3674139265661012 2117 36-37 0.3772179959270794 2117 38-39 -1.1432163906608146 2117 40-41 -1.1211887382801997 2117 42-43 0.16894097459838164 2117 44-45 -0.712089006373084 2117 46-47 0.06558212747194148 2117 48-49 -1.8393958237128807 2117 50-51 -0.6269614554547935 2117 52-53 -0.44233542475746646 2117 54-55 0.5212721622373664 2117 56-57 0.8780155944791872 2117 58-59 -2.9464247046429186 2117 60-61 -1.5035095771351408 2117 62-63 0.4615542224041853 2117 64-65 0.1215342138401958 2117 66-67 3.1118901491357995 2117 68-69 1.8808148090540264 2117 70-71 -0.7518805875731616 2117 72-73 0.20525934503864818 2117 74-75 1.7751623055503032 2117 76 -1.3463181603377663 2118 1 2.8837371026360046 2118 2 1.3404650658364368 2118 3 2.5998278890446898 2118 4 1.1274698940691898 2118 5 2.439104423679428 2118 6 -0.4860058641343805 2118 7 0.7698814817946698 2118 8 0.8130268391018411 2118 9 2.0795014447689084 2118 10-11 2.8030093021537787 2118 12-13 1.9426809449078846 2118 14-15 2.718381542768306 2118 16-17 3.0365146118022146 2118 18-19 0.39930436004289405 2118 20-21 0.2420289958872992 2118 22-23 -1.079986549640541 2118 24-25 -1.171667698365546 2118 26-27 -1.0725850256627218 2118 28-29 2.2473719700007777 2118 30-31 0.602029564602045 2118 32-33 1.9044918572615188 2118 34-35 2.046935350408315 2118 36-37 3.036839797929307 2118 38-39 1.3300872800844594 2118 40-41 0.84265998252069 2118 42-43 0.10275632498770548 2118 44-45 2.408600648799329 2118 46-47 3.8253151641793934 2118 48-49 3.1611603498132617 2118 50-51 0.36580828870538085 2118 52-53 -0.5413343124104131 2118 54-55 0.6914612612362525 2118 56-57 0.6800178191732904 2118 58-59 1.4912838604293874 2118 60-61 1.4308619467803219 2118 62-63 0.5427555572206479 2118 64-65 0.7283195308591068 2118 66-67 1.9711782470223405 2118 68-69 -2.361676848343084 2118 70-71 0.43944310541905196 2118 72-73 2.1307320925358653 2118 74-75 0.7634826615013637 2118 76 1.6948386805966038 2119 1 -0.28216151487551855 2119 2 2.0317093054677713 2119 3 2.1090444789064406 2119 4 1.680465285774261 2119 5 -1.0217250694081272 2119 6 0.23749644001308567 2119 7 -1.8245885642882804 2119 8 3.6321512630649764 2119 9 0.12904061527581945 2119 10-11 1.8174102238127645 2119 12-13 1.2370357836175643 2119 14-15 1.808243293920384 2119 16-17 -0.34770197805953984 2119 18-19 0.3716545904576414 2119 20-21 1.4079276133988223 2119 22-23 0.9084927130322704 2119 24-25 0.6128945136160233 2119 26-27 -0.44931313626180014 2119 28-29 -1.883388398662813 2119 30-31 0.5559466152932906 2119 32-33 0.6130172028836363 2119 34-35 -0.21285727631979512 2119 36-37 -0.34507264446700603 2119 38-39 1.5144190773194772 2119 40-41 1.4256092912764515 2119 42-43 -0.8569210943671344 2119 44-45 0.12288636308504408 2119 46-47 1.4485870535803151 2119 48-49 -0.5093465626291369 2119 50-51 0.8502552933136798 2119 52-53 2.4874675309075585 2119 54-55 1.7484889110058397 2119 56-57 1.3269072200949523 2119 58-59 0.6952009111206294 2119 60-61 -1.7528938135883436 2119 62-63 0.059460626345074985 2119 64-65 3.3481351990618684 2119 66-67 0.3951413806628956 2119 68-69 0.40790840511313675 2119 70-71 -0.12564906047956725 2119 72-73 2.9608012169598297 2119 74-75 0.29055639422025337 2119 76 1.1487557312878494 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 3.0 12 46.0 13 271.0 14 622.0 15 987.0 16 1181.0 17 1436.0 18 1582.0 19 1512.0 20 1315.0 21 978.0 22 661.0 23 503.0 24 301.0 25 204.0 26 138.0 27 105.0 28 82.0 29 65.0 30 61.0 31 41.0 32 28.0 33 27.0 34 21.0 35 10.0 36 18.0 37 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.570226109919128 51.65043736590197 7.27842878362766 35.50090774055125 2 15.74938574938575 29.50859950859951 29.115479115479115 25.626535626535627 3 18.656838656838655 31.31040131040131 22.915642915642916 27.117117117117118 4 22.08026208026208 27.493857493857494 19.844389844389845 30.581490581490584 5 8.222768222768224 34.38165438165438 31.769041769041767 25.626535626535627 6 7.2481572481572485 31.425061425061422 11.752661752661753 49.57411957411957 7 5.192465192465193 49.86895986895987 10.122850122850123 34.81572481572481 8 3.8984438984438983 51.36773136773137 11.76085176085176 32.972972972972975 9 8.902538902538902 35.22522522522522 10.204750204750205 45.66748566748567 10-11 7.366912366912366 34.909909909909906 22.325962325962326 35.3972153972154 12-13 7.047502047502048 30.4013104013104 22.387387387387385 40.16380016380016 14-15 11.404586404586405 33.80835380835381 16.715806715806718 38.07125307125307 16-17 15.192465192465193 32.23177723177723 17.624897624897624 34.95085995085995 18-19 11.498771498771498 30.06552006552007 17.235872235872236 41.1998361998362 20-21 10.417690417690418 30.94185094185094 17.444717444717444 41.1957411957412 22-23 16.846846846846848 30.372645372645373 14.393939393939394 38.386568386568385 24-25 13.124488124488124 35.827190827190826 13.95986895986896 37.08845208845209 26-27 11.036036036036036 36.2981162981163 18.787878787878785 33.877968877968875 28-29 13.841113841113842 32.23177723177723 21.613431613431615 32.31367731367732 30-31 13.607698607698607 31.04832104832105 23.435708435708435 31.908271908271907 32-33 10.192465192465193 36.03603603603604 21.605241605241606 32.16625716625717 34-35 10.405405405405405 34.19328419328419 20.601965601965603 34.7993447993448 36-37 10.827190827190828 30.384930384930385 23.8984438984439 34.88943488943489 38-39 11.392301392301391 25.864045864045863 27.444717444717444 35.2989352989353 40-41 8.480753480753481 27.98116298116298 25.380835380835382 38.157248157248155 42-43 4.815724815724816 26.24897624897625 29.004914004914006 39.93038493038493 44-45 6.597051597051597 28.169533169533167 29.84029484029484 35.39312039312039 46-47 7.313677313677314 31.490581490581494 25.64701064701065 35.54873054873055 48-49 7.612612612612613 34.2997542997543 20.25389025389025 37.83374283374283 50-51 7.461097461097461 32.31367731367732 19.897624897624897 40.32760032760033 52-53 6.294021294021294 31.53153153153153 20.704340704340705 41.47010647010647 54-55 6.936936936936937 31.203931203931205 19.045864045864047 42.813267813267814 56-57 10.012285012285012 31.490581490581494 17.694512694512696 40.8026208026208 58-59 8.890253890253891 30.782145782145783 18.783783783783782 41.54381654381654 60-61 6.363636363636363 28.65683865683866 19.803439803439804 45.176085176085174 62-63 8.177723177723177 30.880425880425882 17.846027846027845 43.09582309582309 64-65 8.935298935298935 32.203112203112205 14.357084357084357 44.5045045045045 66-67 6.867321867321867 39.18509418509418 12.289107289107289 41.65847665847666 68-69 8.64864864864865 38.57084357084357 11.646191646191646 41.13431613431614 70-71 6.5192465192465185 43.071253071253075 11.126126126126126 39.283374283374286 72-73 8.890253890253891 40.09418509418509 12.846027846027846 38.16953316953317 74-75 8.894348894348894 38.77149877149877 15.184275184275183 37.14987714987715 76 10.294840294840295 34.92219492219492 17.272727272727273 37.51023751023751 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.0 12 1.0 13 1.0 14 1.0 15 3.5 16 6.0 17 6.0 18 9.0 19 10.0 20 14.0 21 20.0 22 18.5 23 19.0 24 29.0 25 37.0 26 37.5 27 41.0 28 54.5 29 65.0 30 78.5 31 108.5 32 148.0 33 171.0 34 187.0 35 241.0 36 304.5 37 330.0 38 351.5 39 411.0 40 516.5 41 627.0 42 670.0 43 670.0 44 721.5 45 751.0 46 729.0 47 761.5 48 788.0 49 779.5 50 777.0 51 719.5 52 627.5 53 553.0 54 513.0 55 481.5 56 438.5 57 369.5 58 312.0 59 287.5 60 241.5 61 182.0 62 144.0 63 134.5 64 111.5 65 80.5 66 61.5 67 60.0 68 48.5 69 33.0 70 23.5 71 18.0 72 15.5 73 14.5 74 18.0 75 20.0 76 12.5 77 7.0 78 8.5 79 8.0 80 5.0 81 3.5 82 5.5 83 6.0 84 5.5 85 3.0 86 2.0 87 3.0 88 3.5 89 3.0 90 2.0 91 2.0 92 2.0 93 1.0 94 0.0 95 2.0 96 4.0 97 3.5 98 3.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7534807534807535 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 12210.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.36936936936937 #Duplication Level Percentage of deduplicated Percentage of total 1 99.80219236792219 99.17280917280917 2 0.1153877853787192 0.2293202293202293 3 0.03296793867963405 0.09828009828009827 4 0.024725954009725545 0.09828009828009827 5 0.008241984669908513 0.040950040950040956 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.016483969339817026 0.36036036036036034 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGGTC 26 0.21294021294021295 No Hit CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG 18 0.14742014742014742 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.00819000819000819 0.0 5 0.0 0.0 0.0 0.00819000819000819 0.0 6 0.0 0.0 0.0 0.00819000819000819 0.0 7 0.0 0.0 0.0 0.00819000819000819 0.0 8 0.0 0.0 0.0 0.00819000819000819 0.0 9 0.0 0.0 0.0 0.02457002457002457 0.0 10 0.0 0.0 0.0 0.02457002457002457 0.0 11 0.0 0.0 0.0 0.02457002457002457 0.0 12 0.0 0.0 0.0 0.02457002457002457 0.0 13 0.0 0.0 0.0 0.03276003276003276 0.0 14 0.0 0.0 0.0 0.04095004095004095 0.0 15 0.0 0.0 0.0 0.04095004095004095 0.0 16 0.0 0.0 0.0 0.04095004095004095 0.0 17 0.0 0.0 0.0 0.04095004095004095 0.0 18 0.0 0.0 0.0 0.04095004095004095 0.0 19 0.0 0.0 0.0 0.04914004914004914 0.0 20 0.0 0.0 0.0 0.0819000819000819 0.0 21 0.0 0.0 0.0 0.0819000819000819 0.0 22 0.0 0.0 0.0 0.0819000819000819 0.0 23 0.0 0.0 0.0 0.0819000819000819 0.0 24 0.0 0.0 0.0 0.0819000819000819 0.0 25 0.0 0.0 0.0 0.0819000819000819 0.0 26 0.0 0.0 0.0 0.0819000819000819 0.0 27 0.0 0.0 0.0 0.09009009009009009 0.0 28 0.0 0.0 0.0 0.10647010647010648 0.0 29 0.0 0.0 0.0 0.10647010647010648 0.0 30 0.0 0.0 0.0 0.10647010647010648 0.0 31 0.0 0.0 0.0 0.10647010647010648 0.0 32 0.0 0.0 0.0 0.11466011466011466 0.0 33 0.0 0.0 0.0 0.12285012285012285 0.0 34 0.0 0.0 0.0 0.12285012285012285 0.0 35 0.0 0.0 0.0 0.12285012285012285 0.0 36 0.0 0.0 0.0 0.14742014742014742 0.0 37 0.0 0.0 0.0 0.171990171990172 0.0 38 0.0 0.0 0.0 0.19656019656019655 0.0 39 0.0 0.0 0.0 0.22113022113022113 0.0 40 0.0 0.0 0.0 0.2375102375102375 0.0 41 0.0 0.0 0.0 0.2702702702702703 0.0 42 0.0 0.0 0.0 0.2702702702702703 0.0 43 0.0 0.0 0.0 0.28665028665028663 0.0 44 0.0 0.0 0.0 0.3194103194103194 0.0 45 0.0 0.0 0.0 0.343980343980344 0.0 46 0.0 0.0 0.0 0.3931203931203931 0.0 47 0.0 0.0 0.0 0.43407043407043405 0.0 48 0.0 0.0 0.0 0.45045045045045046 0.0 49 0.0 0.0 0.0 0.4668304668304668 0.0 50 0.0 0.0 0.0 0.5077805077805078 0.0 51 0.0 0.0 0.0 0.5077805077805078 0.0 52 0.0 0.0 0.0 0.5487305487305487 0.0 53 0.0 0.0 0.0 0.5896805896805897 0.0 54 0.0 0.0 0.0 0.6224406224406225 0.0 55 0.0 0.0 0.0 0.647010647010647 0.0 56 0.0 0.0 0.0 0.6715806715806716 0.0 57 0.0 0.0 0.0 0.7125307125307125 0.0 58 0.0 0.0 0.0 0.7371007371007371 0.0 59 0.0 0.0 0.0 0.7534807534807535 0.0 60 0.0 0.0 0.0 0.7698607698607699 0.0 61 0.0 0.0 0.0 0.8108108108108109 0.0 62 0.0 0.0 0.0 0.8353808353808354 0.0 63 0.0 0.0 0.0 0.85995085995086 0.0 64 0.0 0.0 0.0 0.9009009009009009 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCTG 15 0.0021733802 69.991806 11 TAACGCT 15 0.0021733802 69.991806 10 CTAACGC 15 0.0021733802 69.991806 9 CCGAAAA 20 0.0067922757 52.493855 27 GAAAAAC 40 0.002328472 34.995903 29 CATCTGA 80 2.217388E-6 30.621414 9 ACATCTG 80 2.217388E-6 30.621414 8 ATCTGAC 90 5.551512E-6 27.219034 10 TCTGACG 95 8.4515395E-6 25.786455 11 TGACGCT 85 1.2093336E-4 24.70299 13 CACATCT 105 1.8365987E-5 23.3306 7 CTGACGC 105 1.8365987E-5 23.3306 12 GACGCTG 80 0.002522044 21.872438 14 CGCTGCC 90 0.004992614 19.442167 13 >>END_MODULE