FastQCFastQC Report
Wed 25 May 2016
SRR1780490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12210
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT313025.634725634725637No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT251320.58149058149058RNA PCR Primer, Index 27 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC8166.683046683046682No Hit
CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC4103.357903357903358No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG3332.727272727272727No Hit
TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT2221.8181818181818181No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT1281.0483210483210483No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1030.8435708435708437No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1020.8353808353808353No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT1010.8271908271908271No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG940.7698607698607698No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT770.6306306306306306No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC770.6306306306306306No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT670.5487305487305487No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT640.5241605241605242No Hit
CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC580.475020475020475No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTCTT540.4422604422604423No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG530.43407043407043405No Hit
TTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC500.4095004095004095No Hit
CTTATACACATCTCCGAGCCCACGCGACTTGACCCTATCTCGTATGCCGT440.36036036036036034No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG420.343980343980344No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGT370.30303030303030304No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG360.29484029484029484No Hit
ACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCT360.29484029484029484RNA PCR Primer, Index 27 (95% over 23bp)
CTTATCACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC350.28665028665028663No Hit
ATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGTCTT350.28665028665028663TruSeq Adapter, Index 4 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGT320.2620802620802621No Hit
CTTATACACATCTCCGAGCCCACGAGCCTTGACCCTATCTCGTATGCCGT310.2538902538902539No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTCTCTCGTATGCCGTCTT310.2538902538902539No Hit
ATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTT240.19656019656019655Illumina PCR Primer Index 4 (96% over 26bp)
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTC230.18837018837018837No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCG230.18837018837018837No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCAGT220.18018018018018017No Hit
CTTATATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC220.18018018018018017No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTCTGCCGTCTT200.16380016380016382No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTAGTATGCCGT200.16380016380016382No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCG190.15561015561015562No Hit
CTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT190.15561015561015562No Hit
ATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGTCTT180.14742014742014742Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTC180.14742014742014742No Hit
TCTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT180.14742014742014742No Hit
CTTATACACATCTCCGCGCCCACGAGACTTGACCCTATCTCGTATGCCGT170.13923013923013922No Hit
ATACACATCTCCGAGCCCACGAGACTTGCCCCTATCTCGTATGCCGTCTT170.13923013923013922RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTCTCTCGTATGCCGT170.13923013923013922No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTCTGCCGT170.13923013923013922No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCCCCTATCTCGTATGCCGT160.13104013104013104No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCCT160.13104013104013104No Hit
CTTATACACATCTCCGAGCACACGAGACTTGACCCTATCTCGTATGCCGT160.13104013104013104No Hit
CTTATACACATATCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT150.12285012285012285No Hit
CTTATACACATCTCCGAGCCCAAGAGACTTGACCCTATCTCGTATGCCGT140.11466011466011465No Hit
CTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTC140.11466011466011465No Hit
ATACACATCTCCGAGCCCACGAGAATTGACCCTATCTCGTATGCCGTCTT140.11466011466011465No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGACGTCTT140.11466011466011465No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCG130.10647010647010648No Hit
TCTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT130.10647010647010648No Hit
ATACACATCTCCGAGCCCACGAGCCTTGACCCTATCTCGTATGCCGTCTT130.10647010647010648RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA1300.067.307691
TTTACAC1300.067.307692
TTACACA1350.064.814823
CTTATAC5200.063.9423071
TTATACA5500.061.090912
ACAACAA307.895893E-658.33333270
TAATACA252.29819E-456.0000043
TTAATAC252.29819E-456.0000042
CAAACAA252.29819E-456.00000470
CTATACA750.056.01
CGCGACT200.0067891352.523
GCGACTT200.0067891352.524
CACGCGA200.0067891352.521
CTCTTAT200.0067891352.51
CAACACA200.0067891352.569
CTTAATA200.0067891352.51
ACGCGAC200.0067891352.522
TATACAC6600.051.9696963
CCCTAAC404.3318545E-543.7533
ACCCTAA503.2055086E-642.00000432
Produced by FastQC (version 0.11.5)