FastQCFastQC Report
Wed 25 May 2016
SRR1780489_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780489_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71117
Sequences flagged as poor quality0
Sequence length50
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1000.14061335545650125No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC800.112490684365201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATATC207.74827E-444.06699840
AGGCACA357.258541E-637.665462
CTTTACA950.037.004661
AAGGCAC301.2987155E-436.6191981
TATATCT250.00232054835.25359739
TTCTCGG250.002344766635.1791742
CTTATCA250.002352883835.154431
CGAAACT607.777999E-933.003725
CTTATAA401.8122058E-532.957281
CTTATAC5200.032.5347481
CGGTGGT950.032.40186743
TTTACAC1050.031.3878842
GAAACTC651.6965714E-830.4649526
TTATACA5900.029.791892
GAGTGTA300.00566678529.37799634
GGAGATC300.00566678529.37799638
AAACTCG602.8770955E-729.33662227
ATAACAC300.005745444429.2953594
TTACACA1300.028.7319853
TATACAC6450.027.9327833
Produced by FastQC (version 0.11.5)