##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780489_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71117 Sequences flagged as poor quality 0 Sequence length 50 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.115837282225066 16.0 10.0 26.0 7.0 30.0 2 18.114388964663863 16.0 10.0 27.0 7.0 30.0 3 18.170437448148824 16.0 10.0 27.0 7.0 31.0 4 19.669811718717042 17.0 10.0 30.0 7.0 35.0 5 19.693659743802467 17.0 10.0 30.0 7.0 35.0 6 20.337472053095603 17.0 10.0 31.0 7.0 35.0 7 20.398062347961808 17.0 10.0 31.0 7.0 35.0 8 20.325674592572803 17.0 10.0 31.0 7.0 35.0 9 20.518455502903667 17.0 10.0 31.0 7.0 37.0 10 20.478043224545466 17.0 10.0 31.0 7.0 35.0 11 20.53085760085493 17.0 10.0 31.0 7.0 35.0 12 20.519158569680947 17.0 10.0 31.0 7.0 37.0 13 20.39402674466021 17.0 10.0 31.0 7.0 35.0 14 20.629905648438488 17.0 10.0 31.0 7.0 37.0 15 20.590547970246213 17.0 10.0 31.0 7.0 37.0 16 20.305693434762432 17.0 8.0 31.0 7.0 37.0 17 20.44355076845199 17.0 9.0 31.0 7.0 37.0 18 20.112350071009743 17.0 8.0 30.0 7.0 36.0 19 20.095251486986236 18.0 8.0 30.0 7.0 37.0 20 20.19301995303514 18.0 8.0 30.0 7.0 37.0 21 20.049819311838238 18.0 8.0 30.0 7.0 37.0 22 20.052392536243094 18.0 8.0 30.0 7.0 37.0 23 19.873208937384874 17.0 8.0 30.0 7.0 37.0 24 19.402252625954414 17.0 8.0 30.0 7.0 36.0 25 19.312372569146618 16.0 8.0 30.0 7.0 36.0 26 18.95293670992871 16.0 8.0 30.0 7.0 36.0 27 18.794690439697963 16.0 8.0 29.0 7.0 36.0 28 18.66420124583433 16.0 8.0 28.0 7.0 35.0 29 18.456726239858263 15.0 8.0 28.0 7.0 35.0 30 18.15467469100215 15.0 8.0 27.0 7.0 35.0 31 18.09452029753786 15.0 8.0 27.0 7.0 35.0 32 17.774315564492316 15.0 8.0 27.0 7.0 35.0 33 17.82770645555915 15.0 8.0 27.0 7.0 35.0 34 17.48830799949379 15.0 8.0 26.0 7.0 34.0 35 17.336389330258587 14.0 8.0 26.0 7.0 34.0 36 17.235991394462648 14.0 8.0 26.0 7.0 34.0 37 17.01092565771897 13.0 8.0 25.0 6.0 33.0 38 17.03519552287076 13.0 8.0 25.0 7.0 33.0 39 16.69170521816162 13.0 8.0 25.0 6.0 33.0 40 16.21196057201513 13.0 8.0 24.0 6.0 33.0 41 16.36784453787421 13.0 8.0 24.0 7.0 33.0 42 16.386250826103463 13.0 8.0 24.0 7.0 32.0 43 16.380809089247297 13.0 8.0 24.0 7.0 33.0 44 16.388022554382214 13.0 8.0 24.0 7.0 33.0 45 16.441947776199783 13.0 8.0 24.0 7.0 33.0 46 16.275953710083385 13.0 8.0 24.0 7.0 32.0 47 16.17420588607506 13.0 8.0 24.0 7.0 32.0 48 16.16111478268206 13.0 8.0 24.0 7.0 32.0 49 15.85521042788644 12.0 7.0 23.0 7.0 32.0 50 15.824402041705921 12.0 7.0 23.0 7.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -1.3832597047414765 1101 2 -0.9574270665589601 1101 3 -0.1301518685953198 1101 4 -1.1214572482099854 1101 5 -0.10853076818089846 1101 6 -1.402988704115259 1101 7 -0.5933464446964685 1101 8 -1.8634834837416179 1101 9 -1.9454134706846276 1101 10 -1.2960605832208465 1101 11 -0.8677667564969092 1101 12 -2.517869841059717 1101 13 -1.935273547227272 1101 14 -2.14689405958676 1101 15 -2.6766856603271307 1101 16 -2.966230718217851 1101 17 -1.2245403040249947 1101 18 -1.3913165353603212 1101 19 -3.046388085282917 1101 20 -2.6233815587300775 1101 21 -1.987387978902838 1101 22 -2.4239524929684464 1101 23 -2.2484817280050606 1101 24 -2.6925019915500314 1101 25 -0.9754217985628699 1101 26 -1.4965023392904833 1101 27 -1.5890850188502128 1101 28 -0.9163182161457719 1101 29 -1.1820058360409718 1101 30 -1.2698874602331323 1101 31 -2.2793001864877915 1101 32 -2.9462611941224743 1101 33 -1.3230591870575203 1101 34 -1.6713198736478514 1101 35 -2.7511812482423466 1101 36 -0.7018323443260002 1101 37 -1.9902001742101554 1101 38 -0.6301654002318884 1101 39 -0.8355600539792878 1101 40 -1.570659657149644 1101 41 -1.0148552722493118 1101 42 -1.3886721271039981 1101 43 -0.9952350069576763 1101 44 -0.6432399059489509 1101 45 -1.201693096210965 1101 46 -1.8979777180446362 1101 47 -2.190514210815385 1101 48 -0.8509836500976391 1101 49 -1.8326192524642373 1101 50 -2.16651506042132 1102 1 0.8036561831089912 1102 2 1.6407037745625352 1102 3 0.5988200940215016 1102 4 2.0187296676778637 1102 5 1.5643664280807847 1102 6 0.09233839868847937 1102 7 2.173008695490445 1102 8 1.0617501611181943 1102 9 1.334960361091074 1102 10 0.8067431550969104 1102 11 0.646251935091879 1102 12 1.398018009407572 1102 13 1.5693993499689896 1102 14 2.535348931067446 1102 15 1.6784545265887587 1102 16 1.1833019920625212 1102 17 1.6820017520497714 1102 18 1.9825152403406143 1102 19 0.8788455595768987 1102 20 1.5635343291203903 1102 21 1.0593409930597808 1102 22 0.6695054509567875 1102 23 1.8730135990977423 1102 24 2.176656886954639 1102 25 1.7722417528389975 1102 26 2.251161212111384 1102 27 0.9716626447011905 1102 28 1.2238686997420771 1102 29 1.8273399583515513 1102 30 0.9637573995799507 1102 31 1.3935970097738917 1102 32 3.1471967498027595 1102 33 1.8545109064004244 1102 34 2.132418444109156 1102 35 3.0245196863370936 1102 36 2.5972330762347475 1102 37 2.72008019962162 1102 38 2.4352551605157764 1102 39 2.1177109740580953 1102 40 2.0835459503269895 1102 41 2.200097998778727 1102 42 3.1253465644847864 1102 43 1.883269665939519 1102 44 1.3287227108734783 1102 45 2.2749424178077255 1102 46 1.653424151114244 1102 47 1.0992054153528414 1102 48 2.4200443872855395 1102 49 3.017848037255389 1102 50 2.7400269956534444 1103 1 0.00549104549431334 1103 2 -0.5843669619546574 1103 3 -0.5341645582977961 1103 4 0.11390243626227559 1103 5 -0.5711047199943238 1103 6 -0.2669173674094374 1103 7 0.3247706778277504 1103 8 0.7317321554592731 1103 9 -0.5161928584922251 1103 10 -0.5786636870534778 1103 11 0.10934033430305945 1103 12 -0.9687829851907317 1103 13 -0.3487177725552293 1103 14 -0.9115343751144991 1103 15 0.5426404135818146 1103 16 0.5465875961095072 1103 17 -0.3498082453779894 1103 18 -0.7567456702312789 1103 19 -0.6801015380823685 1103 20 -0.2140529125841475 1103 21 -0.9276263395304625 1103 22 -0.7483973184850363 1103 23 -0.478782679732749 1103 24 -1.5963003281701127 1103 25 0.0250069076019237 1103 26 0.593440214083433 1103 27 -0.09049974919403425 1103 28 -1.1046917049565437 1103 29 0.09588149997891904 1103 30 0.9504675084713163 1103 31 0.017535962016026474 1103 32 -0.16318651350856683 1103 33 0.739531913002498 1103 34 0.6710448695571571 1103 35 0.9792483153347789 1103 36 0.2943093001908501 1103 37 -0.8634746318968567 1103 38 -1.5757197173029667 1103 39 -1.0455403334957083 1103 40 -0.7962448410645884 1103 41 -0.5917222875969923 1103 42 -2.205357371037806 1103 43 -1.6820222829587053 1103 44 -1.677193434200687 1103 45 -1.3361353494905686 1103 46 -1.1347092622442414 1103 47 -1.9074824008179565 1103 48 -1.4013566244610107 1103 49 -1.65204821585273 1103 50 0.8457459358918058 1104 1 0.03950523971276354 1104 2 0.7328392004133555 1104 3 -0.13940949511621525 1104 4 -0.9924676520188385 1104 5 0.27552848062883584 1104 6 -0.053138589497027766 1104 7 0.717621638534002 1104 8 -0.4053632227647199 1104 9 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0.22327393640689408 1104 45 0.25422296797524524 1104 46 -0.06399782031936851 1104 47 0.14037981140291933 1104 48 0.45557603955145254 1104 49 -0.16130907789185045 1104 50 -0.932253025506844 1105 1 -0.7677723883195817 1105 2 -0.4494608894339045 1105 3 -1.1297958383327469 1105 4 0.1294550793378555 1105 5 -2.018811142012673 1105 6 -0.6299579965051123 1105 7 -1.0131061242692319 1105 8 -0.8051835282454007 1105 9 -0.6481282014367409 1105 10 -0.793167837337446 1105 11 -0.272573165041635 1105 12 -1.3926361961999056 1105 13 -1.7632219228391968 1105 14 -1.105505541562728 1105 15 -0.7329384418135554 1105 16 -0.7366802064243494 1105 17 -0.8945002506204531 1105 18 -2.31672819535142 1105 19 -1.5599617390434837 1105 20 -0.10544653425299799 1105 21 -0.07799768072749202 1105 22 -0.5919097693369366 1105 23 -1.1427407400210825 1105 24 -2.268558956392045 1105 25 -1.0234858840101353 1105 26 -0.8755855613643604 1105 27 -0.5066640130647215 1105 28 -0.8160066896660219 1105 29 -2.4990507848616232 1105 30 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0.15857198374213866 2212 1 0.05590843536124268 2212 2 0.8473216073485901 2212 3 1.0583637498268423 2212 4 1.1515915014742788 2212 5 0.757765434563904 2212 6 1.2426288760817599 2212 7 0.15574844458533477 2212 8 0.7199129968511215 2212 9 -0.2015387548472951 2212 10 1.2750432308736208 2212 11 -0.1920068844753544 2212 12 -0.7007443043080137 2212 13 0.2977819381646647 2212 14 0.8648934288362398 2212 15 1.2585673496922354 2212 16 1.6445295747854303 2212 17 0.8807893246691201 2212 18 0.43153435291112885 2212 19 1.5288413497596025 2212 20 0.17151614270968096 2212 21 0.3101619074320965 2212 22 0.10101172358455557 2212 23 0.3082245109441679 2212 24 0.9018400140069254 2212 25 0.3587534982292482 2212 26 0.40086231508351844 2212 27 0.6874157810150727 2212 28 0.4975068238474911 2212 29 0.29168280587196804 2212 30 0.631350226051282 2212 31 0.13250414120438592 2212 32 0.07815574618231835 2212 33 0.08815576621350729 2212 34 0.7308018742682876 2212 35 -0.7310583215773647 2212 36 -0.02472533077038719 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2213 24 0.7890207727473175 2213 25 1.2543647057670633 2213 26 0.2723392966522411 2213 27 1.3498672232830309 2213 28 -0.2239577600024134 2213 29 1.7472576467679097 2213 30 1.124779435076821 2213 31 0.18267357669492412 2213 32 0.3138777066749192 2213 33 0.20742182125938058 2213 34 0.24902652093330246 2213 35 1.1352116180870695 2213 36 0.5970615937688315 2213 37 -0.23962142088768346 2213 38 0.07565642948602402 2213 39 1.4040009509079638 2213 40 1.029443232329907 2213 41 0.4174520243296129 2213 42 0.01482611520072652 2213 43 0.666601570252304 2213 44 0.9558340887350916 2213 45 0.645516026656221 2213 46 1.6267586276640138 2213 47 0.6704992505581906 2213 48 0.9564415406766038 2213 49 0.4947407749729589 2213 50 0.3595074037817163 2214 1 1.1664880415160717 2214 2 0.7504548290658839 2214 3 1.674165392177155 2214 4 1.5419287413690377 2214 5 1.6148171765713961 2214 6 1.5659964914847748 2214 7 1.5148331930506131 2214 8 1.6819635429724649 2214 9 1.4028621061907351 2214 10 0.762247249051093 2214 11 0.8889944708622863 2214 12 0.8763903524726615 2214 13 1.178033871513943 2214 14 0.7642795406117351 2214 15 0.9137535093031737 2214 16 1.048739094039199 2214 17 1.5368524674579263 2214 18 0.5069549062077883 2214 19 1.5533968787399104 2214 20 2.2425517801169974 2214 21 1.5395328713163359 2214 22 1.8495567287667285 2214 23 1.8195854293847304 2214 24 2.4354444148679626 2214 25 1.3001550767989691 2214 26 1.1531544302074899 2214 27 0.8006263367035054 2214 28 1.7686540772956292 2214 29 1.1442699062466772 2214 30 0.7837061217700096 2214 31 2.101809729979003 2214 32 1.86847704092839 2214 33 1.8492177131161611 2214 34 -0.22040597074486712 2214 35 0.06165474547198713 2214 36 -0.36786493054715663 2214 37 0.3057224128380618 2214 38 0.0318145848810083 2214 39 1.5744970132608067 2214 40 1.4137088814327718 2214 41 1.3335030107711177 2214 42 2.053309512131797 2214 43 1.9022920793048321 2214 44 0.9310715653933705 2214 45 1.2499651619976184 2214 46 2.0629849814839396 2214 47 2.274542442893985 2214 48 0.8926273001959686 2214 49 0.743015517199149 2214 50 0.8424172032458692 2215 1 -0.6524841677682005 2215 2 -0.371265400712101 2215 3 -0.017264510011944623 2215 4 -0.11014391815587743 2215 5 0.3566274544785557 2215 6 0.3813201989836088 2215 7 -0.8805246706351468 2215 8 -0.20493290167898692 2215 9 0.3067590166083711 2215 10 -0.40845605788882366 2215 11 -0.6154303078987766 2215 12 0.07403047046676292 2215 13 -0.07324698549583886 2215 14 0.8468754108182246 2215 15 0.9257081747277986 2215 16 0.815700745956601 2215 17 -0.26216942355278405 2215 18 0.23665526329519793 2215 19 -1.3732449470635366 2215 20 -0.13953174728557727 2215 21 -0.2216067032838822 2215 22 0.8587167970791008 2215 23 0.18257254318693583 2215 24 0.36627813633978334 2215 25 1.0479426874184377 2215 26 1.5194019073073193 2215 27 0.2592507739026999 2215 28 0.2997827840181877 2215 29 1.09443292441156 2215 30 0.6166513166155312 2215 31 1.5473927139876977 2215 32 0.766396618633717 2215 33 1.3513136609503498 2215 34 1.6542252976917133 2215 35 2.2326685632021537 2215 36 0.7720978081580157 2215 37 1.8110787239698745 2215 38 1.863440108867966 2215 39 1.1327954141302392 2215 40 1.1963513282578013 2215 41 0.03712046147752801 2215 42 -0.41556178114564446 2215 43 -0.01286084725936476 2215 44 0.006292804331422985 2215 45 0.2606777842612438 2215 46 0.28787242132247215 2215 47 0.19963171474618235 2215 48 0.6712331067477457 2215 49 0.754116277950585 2215 50 0.8087267825037507 2216 1 -0.7653093341323896 2216 2 -0.7894444690726594 2216 3 -1.4333262160010563 2216 4 -0.4495268582647718 2216 5 -0.9395007971850866 2216 6 -0.7802043019238312 2216 7 -1.5818253360918924 2216 8 -1.9759552852240567 2216 9 -1.8977176924055428 2216 10 -1.1375849145131447 2216 11 -1.6326716872091218 2216 12 -1.834015506544734 2216 13 -2.019707965532138 2216 14 -1.828411945503614 2216 15 -1.878587046082849 2216 16 -1.7676325873767311 2216 17 -1.9481948319012439 2216 18 -1.4882388685862296 2216 19 -1.34239195250818 2216 20 -1.2029013782595648 2216 21 -1.7648613917291343 2216 22 -2.304471345691617 2216 23 -0.8860904937156633 2216 24 -0.8891665097582191 2216 25 -1.3486090367194947 2216 26 -0.9394607666188755 2216 27 -1.3730843743126684 2216 28 -0.4499147356430342 2216 29 -1.3043874022672064 2216 30 -1.5105416658406092 2216 31 0.027580251797740374 2216 32 0.1795847614044348 2216 33 -1.2997752682692507 2216 34 -0.24519998966460932 2216 35 0.6692989322281662 2216 36 0.0016803852905020733 2216 37 0.12323843358874953 2216 38 -0.387013611640203 2216 39 -0.5381059772793186 2216 40 -0.38229355982447544 2216 41 -0.5058317466289441 2216 42 -0.811811024944797 2216 43 -0.7354863765999582 2216 44 -0.5799140922203012 2216 45 -0.7012096930527321 2216 46 -0.7395020493369344 2216 47 0.07092633059615139 2216 48 -0.13079351152206797 2216 49 -0.3794287916198922 2216 50 0.1999851007130644 >>END_MODULE >>Per sequence quality scores fail #Quality Count 3 2.0 4 2.0 5 4.0 6 44.0 7 686.0 8 3096.0 9 5495.0 10 4943.0 11 4057.0 12 3694.0 13 3776.0 14 3678.0 15 3581.0 16 3467.0 17 3144.0 18 2847.0 19 2591.0 20 2320.0 21 2207.0 22 2006.0 23 1920.0 24 1815.0 25 1682.0 26 1576.0 27 1505.0 28 1467.0 29 1271.0 30 1275.0 31 1192.0 32 1106.0 33 1030.0 34 935.0 35 826.0 36 661.0 37 548.0 38 366.0 39 302.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.315465716980338 34.25627454568488 8.699201869343055 27.729057867991724 2 17.212284846439417 33.33755203059962 30.991956350545617 18.458206772415345 3 27.97542043984476 25.88306428933011 25.960402722312843 20.18111254851229 4 29.728893638562347 23.011699195680297 21.674447381742507 25.58495978401485 5 23.679622025985715 29.503909106248944 24.952190786883403 21.864278080881938 6 29.081654175513588 35.476749581675264 11.769337851709155 23.672258391101984 7 27.06142077732156 29.264863040665958 12.250407784464818 31.423308397547668 8 23.21948338653205 36.83085619472137 15.577147517471209 24.372512901275364 9 27.044927230542083 25.039724390072415 11.847008366729945 36.06834001265556 10 23.05669610214298 28.594127903706617 25.025310759885254 23.323865234265146 11 32.59273842000169 22.818291756897377 18.81907922490649 25.76989059819445 12 24.357730436616748 25.226745412360263 22.083948534064543 28.33157561695845 13 28.243169129951767 19.66362447441324 21.195033117239245 30.898173278395745 14 36.16330089441413 20.894976655228668 15.978511559880745 26.963210890476457 15 27.692069697779402 25.952437875314665 17.26693574472274 29.088556682183185 16 33.25821490443527 20.54817673488359 18.33687798419696 27.856730376484172 17 40.23344114751793 19.874841794403036 15.024609759527493 24.86710729855154 18 34.4562905792713 17.847133219723112 16.617607706716615 31.078968494288972 19 31.176918091910625 18.880843030627485 20.13609083993125 29.806148037530644 20 36.60139012251689 18.73845676662578 19.093741628952895 25.56641148190444 21 28.10499901316717 21.161925169876223 16.16291200270674 34.57016381424987 22 34.648715567935625 17.43901409639571 15.041782729805014 32.87048760586365 23 42.65984079210149 19.08807065905319 15.078338162077015 23.173750386768305 24 30.64382153768179 28.914950231595544 14.648946205071168 25.79228202565149 25 37.987985875666475 20.66316840876159 14.235471209712589 27.11337450585935 26 35.69318629381647 26.34473639790694 14.67113036628594 23.290946941990658 27 31.63128947405438 26.644722960712325 17.80956801845522 23.914419546778074 28 38.475820744366615 23.332489380257122 16.18280023630686 22.0088896390694 29 33.6043551041652 22.733474939864116 19.582495182095684 24.079674773875002 30 38.270145514931464 20.899828309268482 17.731993582707084 23.098032593092967 31 34.9418269298406 20.478046172676244 20.698920949339485 23.881205948143666 32 31.749827675946374 23.14206535653495 20.130262917270386 24.977844050248287 33 36.7855384398959 20.56974045157206 20.85531406063164 21.7894070479004 34 31.318318022593306 22.26833420086378 21.557897106199793 24.85545067034312 35 34.68285340498263 22.697539634532333 19.822189711198952 22.797417249286088 36 37.50052758198624 19.284176761068668 21.488766020906315 21.726529636038773 37 32.91507828215336 20.253485068013337 24.04872765125406 22.782708998579245 38 34.97685934137043 17.85417868245952 23.611912163968096 23.55704981220195 39 32.5858503439641 19.278871178762856 26.350885584457608 21.78439289281544 40 27.42205880281194 31.345533437583644 21.83621430483355 19.39619345477086 41 31.62929409447711 16.2662127563796 26.33148580592522 25.773007343218072 42 25.226150448080357 19.635345591648964 32.18581618153937 22.952687778731306 43 25.742017161344776 17.091011394007598 29.157406104937404 28.009565339710225 44 26.015894226035584 16.25993389127224 32.871509951473385 24.85266193121879 45 25.875601316566797 18.353166230624243 32.97324668748418 22.797985765324782 46 25.77287687417368 19.378885482010748 28.12456046583589 26.72367717797969 47 30.46358547357169 20.72772792483614 27.45506202706124 21.35362457453093 48 30.079608427803876 21.727756055022645 23.59981996680638 24.5928155503671 49 28.22582913724718 23.682804016990634 24.293229064108697 23.79813778165349 50 29.05889137375698 23.34275707835774 22.722477741676865 24.87587380620842 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 1.5 8 3.0 9 2.5 10 2.0 11 3.5 12 5.0 13 7.0 14 9.0 15 12.0 16 15.0 17 16.5 18 18.0 19 24.5 20 31.0 21 50.5 22 70.0 23 85.0 24 100.0 25 123.5 26 147.0 27 188.0 28 229.0 29 291.0 30 353.0 31 446.0 32 539.0 33 645.5 34 752.0 35 931.0 36 1110.0 37 1279.5 38 1449.0 39 1615.0 40 1781.0 41 1962.5 42 2144.0 43 2474.0 44 2804.0 45 3135.0 46 3466.0 47 3710.5 48 3955.0 49 4267.5 50 4580.0 51 4819.0 52 5058.0 53 5174.5 54 5291.0 55 5172.5 56 5054.0 57 4996.5 58 4939.0 59 4585.5 60 4232.0 61 4006.5 62 3781.0 63 3626.5 64 3472.0 65 3149.0 66 2826.0 67 2665.5 68 2505.0 69 2315.5 70 2126.0 71 2019.0 72 1912.0 73 1748.0 74 1584.0 75 1470.5 76 1357.0 77 1189.5 78 1022.0 79 898.0 80 774.0 81 676.0 82 578.0 83 507.0 84 436.0 85 350.0 86 264.0 87 211.5 88 159.0 89 123.0 90 87.0 91 61.0 92 35.0 93 30.0 94 25.0 95 21.0 96 17.0 97 13.0 98 9.0 99 10.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10686615014694097 2 0.007030667772825063 3 0.0014061335545650126 4 0.0014061335545650126 5 0.0014061335545650126 6 0.0 7 0.0014061335545650126 8 0.0 9 0.002812267109130025 10 0.0014061335545650126 11 0.004218400663695038 12 0.002812267109130025 13 0.008436801327390076 14 0.012655201991085115 15 0.014061335545650125 16 0.1659237594386715 17 0.009842934881955088 18 0.16029922522041143 19 0.1898280298662767 20 0.2643531082582224 21 0.2573224404853973 22 0.049214674409775436 23 0.02109200331847519 24 0.1237397528017211 25 0.04780854085521043 26 0.03515333886412531 27 0.03655947241869033 28 0.03234107175499529 29 0.03937173952782035 30 0.08296187971933575 31 0.05062080796434046 32 0.04218400663695038 33 0.0449962737460804 34 0.04780854085521043 35 0.04218400663695038 36 0.05343307507347048 37 0.03937173952782035 38 0.04218400663695038 39 0.04780854085521043 40 0.1884218963117117 41 0.04359014019151539 42 0.05062080796434046 43 0.03796560597325534 44 0.030934938200430275 45 0.03234107175499529 46 0.02671653753673524 47 0.02671653753673524 48 0.02671653753673524 49 0.02671653753673524 50 0.02812267109130025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 71117.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.13289368224194 #Duplication Level Percentage of deduplicated Percentage of total 1 98.9721763803237 96.13453885850079 2 0.657228061032456 1.2767692675450315 3 0.14765916789715972 0.4302768676968939 4 0.07672486175048496 0.29810031356778266 5 0.040533889226671296 0.19685869763910174 6 0.018819305712383102 0.10967841725607098 7 0.015924027910478013 0.10827228370150596 8 0.015924027910478013 0.1237397528017211 9 0.00868583340571528 0.07593121194651067 >10 0.040533889226671296 0.8099329274294473 >50 0.005790555603810185 0.4359014019151539 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 100 0.14061335545650125 No Hit ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC 80 0.112490684365201 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004218400663695038 0.0 2 0.0 0.0 0.0 0.004218400663695038 0.0 3 0.0 0.0 0.0 0.00562453421826005 0.0 4 0.0 0.0 0.0 0.019685869763910176 0.0 5 0.0 0.0 0.0 0.026716537536735238 0.0 6 0.0 0.0 0.0 0.026716537536735238 0.0 7 0.0 0.0 0.0 0.026716537536735238 0.0 8 0.0 0.0 0.0 0.030934938200430275 0.0 9 0.0 0.0 0.0 0.04218400663695038 0.0 10 0.0 0.0 0.0 0.0449962737460804 0.0 11 0.0 0.0 0.0 0.04640240730064542 0.0 12 0.0 0.0 0.0 0.04640240730064542 0.0 13 0.0 0.0 0.0 0.0562453421826005 0.0 14 0.0 0.0 0.0 0.06468214350999058 0.0 15 0.0 0.0 0.0 0.0660882770645556 0.0 16 0.0 0.0 0.0 0.0660882770645556 0.0014061335545650126 17 0.0 0.0 0.0 0.07171281128281565 0.0014061335545650126 18 0.0 0.0 0.0 0.07593121194651067 0.0014061335545650126 19 0.0 0.0 0.0 0.08436801327390075 0.0014061335545650126 20 0.0 0.0 0.0 0.08718028038303079 0.0014061335545650126 21 0.0 0.0 0.0 0.0885864139375958 0.0014061335545650126 22 0.0 0.0 0.0 0.09280481460129084 0.0014061335545650126 23 0.0 0.0 0.0 0.09702321526498586 0.0014061335545650126 24 0.0 0.0 0.0 0.09702321526498586 0.0014061335545650126 25 0.0 0.0 0.0 0.1012416159286809 0.0014061335545650126 26 0.0 0.0 0.0 0.11108455081063599 0.0014061335545650126 27 0.0 0.0 0.0 0.12092748569259108 0.0014061335545650126 28 0.0 0.0 0.0 0.14061335545650125 0.0014061335545650126 29 0.0 0.0 0.0 0.1546746910021514 0.0014061335545650126 30 0.0 0.0 0.0 0.17436056076606157 0.0014061335545650126 31 0.0 0.0 0.0 0.19264029697540672 0.0014061335545650126 32 0.0 0.0 0.0 0.2109200331847519 0.0014061335545650126 33 0.0 0.0 0.0 0.24044883783061716 0.0014061335545650126 34 0.0 0.0 0.0 0.2995064471223477 0.0014061335545650126 35 0.0 0.0 0.0 0.3670008577414683 0.0014061335545650126 36 0.0 0.0 0.0 0.4654302065610192 0.0014061335545650126 37 0.0 0.0 0.0 0.5976067606901303 0.0014061335545650126 38 0.0 0.0014061335545650126 0.0 0.7480630510285867 0.0014061335545650126 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATATC 20 7.74827E-4 44.066998 40 AGGCACA 35 7.258541E-6 37.66546 2 CTTTACA 95 0.0 37.00466 1 AAGGCAC 30 1.2987155E-4 36.619198 1 TATATCT 25 0.002320548 35.253597 39 TTCTCGG 25 0.0023447666 35.17917 42 CTTATCA 25 0.0023528838 35.15443 1 CGAAACT 60 7.777999E-9 33.0037 25 CTTATAA 40 1.8122058E-5 32.95728 1 CTTATAC 520 0.0 32.534748 1 CGGTGGT 95 0.0 32.401867 43 TTTACAC 105 0.0 31.387884 2 GAAACTC 65 1.6965714E-8 30.46495 26 TTATACA 590 0.0 29.79189 2 GAGTGTA 30 0.005666785 29.377996 34 GGAGATC 30 0.005666785 29.377996 38 AAACTCG 60 2.8770955E-7 29.336622 27 ATAACAC 30 0.0057454444 29.295359 4 TTACACA 130 0.0 28.731985 3 TATACAC 645 0.0 27.932783 3 >>END_MODULE